##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936551.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70812
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06258826187652	33.0	31.0	34.0	30.0	34.0
2	32.20171722306954	33.0	31.0	34.0	30.0	34.0
3	32.09422131842061	33.0	31.0	34.0	30.0	34.0
4	35.800132745862285	37.0	35.0	37.0	35.0	37.0
5	35.879003558718864	37.0	35.0	37.0	35.0	37.0
6	35.85670507823533	37.0	35.0	37.0	35.0	37.0
7	26.727433203411852	36.0	10.0	37.0	0.0	37.0
8	31.110630966502853	36.0	19.0	37.0	17.0	37.0
9	36.040049709088855	39.0	32.0	39.0	32.0	39.0
10	36.77985369711348	38.0	35.0	39.0	34.0	39.0
11	37.04732248771395	39.0	37.0	39.0	34.0	39.0
12	36.86965486075806	39.0	35.0	39.0	33.0	39.0
13	36.62856577981133	39.0	35.0	39.0	33.0	39.0
14	37.81635880924137	40.0	36.0	41.0	33.0	41.0
15	37.96509066259956	40.0	36.0	41.0	33.0	41.0
16	37.9901570355307	40.0	36.0	41.0	33.0	41.0
17	37.882195108173754	40.0	36.0	41.0	33.0	41.0
18	37.81703666045303	40.0	36.0	41.0	33.0	41.0
19	37.67930576738406	39.0	36.0	41.0	33.0	41.0
20	37.739337965316615	39.0	35.0	41.0	33.0	41.0
21	37.670394848330794	39.0	35.0	41.0	33.0	41.0
22	37.65035587188612	39.0	35.0	41.0	33.0	41.0
23	37.65007343388126	39.0	35.0	41.0	33.0	41.0
24	37.562178726769474	39.0	35.0	41.0	33.0	41.0
25	37.46723719143648	39.0	35.0	41.0	33.0	41.0
26	37.31385923289838	39.0	35.0	41.0	33.0	41.0
27	37.18862622154437	39.0	35.0	41.0	33.0	41.0
28	37.16604530305598	39.0	35.0	41.0	33.0	41.0
29	37.176467265435235	39.0	35.0	41.0	33.0	41.0
30	37.13153137886234	39.0	35.0	41.0	32.0	41.0
31	37.00103089871773	39.0	35.0	41.0	32.0	41.0
32	36.82671016211941	39.0	35.0	41.0	32.0	41.0
33	36.70125120036152	39.0	35.0	41.0	31.0	41.0
34	36.5710755239225	39.0	35.0	41.0	31.0	41.0
35	36.50043777890753	39.0	35.0	41.0	31.0	41.0
36	36.34134045077106	38.0	35.0	40.0	30.0	41.0
37	36.279274699203526	38.0	35.0	40.0	30.0	41.0
38	36.1480116364458	38.0	35.0	40.0	30.0	41.0
39	36.1543664915551	38.0	35.0	40.0	30.0	41.0
40	35.913842286618085	38.0	35.0	40.0	29.0	41.0
41	35.883847370502174	38.0	35.0	40.0	29.0	41.0
42	35.805018923346324	38.0	35.0	40.0	29.0	41.0
43	35.782889905665705	38.0	35.0	40.0	29.0	41.0
44	35.559453200022595	38.0	34.0	40.0	29.0	41.0
45	35.41275490029938	38.0	34.0	40.0	28.0	41.0
46	35.266790939388805	37.0	34.0	40.0	27.0	41.0
47	35.17461729650342	37.0	34.0	40.0	27.0	41.0
48	35.13631870304468	37.0	34.0	40.0	27.0	41.0
49	34.96873411286223	37.0	34.0	40.0	27.0	41.0
50	34.7706744619556	37.0	34.0	40.0	26.0	41.0
51	32.66802236908998	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	4.0
11	3.0
12	3.0
13	4.0
14	2.0
15	1.0
16	4.0
17	17.0
18	30.0
19	45.0
20	65.0
21	135.0
22	194.0
23	289.0
24	376.0
25	543.0
26	630.0
27	825.0
28	953.0
29	1188.0
30	1518.0
31	1808.0
32	2376.0
33	3470.0
34	6225.0
35	8040.0
36	6402.0
37	9949.0
38	12605.0
39	13099.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.851833022651526	18.642320510647913	23.853301700276788	16.652544766423773
2	28.02491103202847	26.24131503135062	27.765068067559174	17.96870586906174
3	30.88459583121505	25.950403886346944	27.801785008190706	15.363215274247302
4	27.726938936903352	25.628424560808906	27.97266000112975	18.671976501157996
5	25.874145625035304	29.47381799694967	25.788002033553635	18.86403434446139
6	26.86973959215952	34.96017624131503	24.214822346494945	13.955261820030504
7	61.906174094786195	28.338417217420776	6.066768344348415	3.6886403434446136
8	84.74269897757442	5.23781280009038	6.688131955035869	3.3313562672993275
9	80.27029317064904	5.9156640117494215	8.22177032141445	5.592272496187086
10	49.37298762921539	23.00598768570299	14.530023159916396	13.091001525165227
11	38.59374117381235	21.32830593684686	23.371744901994013	16.706207987346776
12	34.56476303451392	21.418686098401402	25.858611534768116	18.157939332316555
13	23.308196350900978	30.048579336835562	29.31565271422923	17.32757159803423
14	18.5759475795063	31.77568773654183	28.28192961644919	21.366435067502685
15	17.5916511325764	23.94085748178275	40.10902106987516	18.35847031576569
16	21.342427837089758	21.907303846805625	35.66203468338699	21.088233632717618
17	22.144551770886288	21.85787719595549	27.783426537874938	28.214144495283282
18	25.440603287578377	22.970682935095745	29.418742586002374	22.169971191323505
19	25.741399762752078	26.817488561260806	25.273964864712195	22.167146811274925
20	28.716884143930404	24.9590464892956	27.088629045924417	19.235440320849573
21	24.99858780997571	26.806191041066484	26.208834660791958	21.98638648816585
22	23.834943229961024	23.374569282042593	27.147941026944583	25.6425464610518
23	22.154437101056317	26.268146641812123	27.256679658814893	24.32073659831667
24	23.457888493475682	22.282946393266677	32.449302378128	21.80986273512964
25	20.27763655877535	25.895328475399648	29.73930972151613	24.087725244308874
26	19.992374173868836	29.65881489013162	27.30045754956787	23.04835338643168
27	20.41179461108287	29.874879963847935	27.769304637632043	21.944020787437157
28	19.279218211602554	26.91634186296108	32.973224877139465	20.8312150482969
29	20.541716093317515	24.285431847709425	31.332260068914874	23.840591990058183
30	21.314184036603965	27.119697226458793	30.05422809693272	21.51189064000452
31	27.006722024515618	25.29797209512512	25.683499971756202	22.011805908603062
32	27.38236457097667	26.879624922329548	25.474495848161325	20.263514658532454
33	25.377054736485345	26.971417273908376	24.809354346720895	22.842173642885385
34	22.14878834095916	26.817488561260806	27.575834604304355	23.457888493475682
35	21.411625148279953	26.947410043495452	28.67028187312885	22.970682935095745
36	25.16240185279331	27.503812913065584	26.70168897926905	20.632096254872057
37	23.010224255775857	27.184657967576115	28.543184770942776	21.261933005705245
38	22.09653731006044	28.235327345647633	26.087386318703043	23.580749025588883
39	22.676947410043496	27.588544314522963	27.280686889227812	22.45382138620573
40	23.247472179856523	25.141219002428965	27.803197198214992	23.80811161949952
41	19.567304976557644	25.627012370784612	28.90470541716093	25.900977235496807
42	22.861944303225442	23.949330621928485	27.73682426707338	25.45190080777269
43	24.00864260294865	25.51403716884144	26.09585945884878	24.381460769361123
44	22.462294526351464	27.256679658814893	26.87397616223239	23.407049652601252
45	21.308535276506806	29.283172343670564	25.29514771507654	24.113144664746088
46	23.363271761848274	28.345478167542225	26.100096028921655	22.19115404168785
47	20.754956786985257	26.443258204824044	29.948313845110995	22.853471163079703
48	23.133084787889057	23.789753149183753	30.171439868948767	22.905722193978423
49	22.91843190419703	23.26018189007513	30.14602044851155	23.67536575721629
50	20.40473366096142	25.964525786589842	29.28458453369485	24.346156018753884
51	20.722476416426595	26.931875953228268	26.889510252499576	25.45613737784556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	91.5
2	142.0
3	257.5
4	373.0
5	269.0
6	165.0
7	142.0
8	119.0
9	141.5
10	164.0
11	142.5
12	121.0
13	127.5
14	134.0
15	131.5
16	129.0
17	143.0
18	157.0
19	154.5
20	152.0
21	174.5
22	197.0
23	233.0
24	269.0
25	307.5
26	484.0
27	622.0
28	782.5
29	943.0
30	1029.5
31	1116.0
32	1317.5
33	1519.0
34	1734.5
35	1950.0
36	2060.5
37	2171.0
38	2471.5
39	2772.0
40	3331.5
41	3891.0
42	4583.5
43	5276.0
44	5483.0
45	5690.0
46	5980.0
47	6270.0
48	5929.5
49	5589.0
50	5309.5
51	5030.0
52	4806.5
53	4583.0
54	4125.0
55	3667.0
56	3414.5
57	3162.0
58	3172.0
59	3182.0
60	3017.5
61	2853.0
62	2579.5
63	2306.0
64	2075.0
65	1844.0
66	1520.5
67	1197.0
68	1069.5
69	942.0
70	794.5
71	647.0
72	555.0
73	463.0
74	357.5
75	218.0
76	184.0
77	141.0
78	98.0
79	71.5
80	45.0
81	27.5
82	10.0
83	12.5
84	15.0
85	10.0
86	5.0
87	7.0
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	70812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.85821612156132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.42845899575003	29.798621702536295
2	10.876751141193138	9.758233067841608
3	6.28994175979852	8.464667005592272
4	4.092554698567606	7.343388126306276
5	3.0725641429246027	6.891487318533581
6	2.30757122619235	6.210811726825962
7	1.7786872343774593	5.585211546065639
8	1.3536911695262082	4.857933683556459
9	1.0514717456319849	4.245043213014743
>10	2.723122934046907	15.391459074733097
>50	0.012592475995592633	0.36010845619386544
>100	0.012592475995592633	1.0930350788002035
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	230	0.32480370558662375	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	211	0.2979720951251201	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	192	0.27114048466361634	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.19911879342484323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0847314014573801	0.0
2	0.0	0.0	0.0	0.4208326272383212	0.0
3	0.0	0.0	0.0	0.532395639157205	0.0
4	0.0	0.0	0.0	0.7060950121448342	0.0
5	0.0	0.0	0.0	1.4376094447268823	0.0
6	0.0	0.0	0.0	1.7200474495848161	0.0
7	0.0	0.0	0.0	2.073094955657233	0.0
8	0.0	0.0	0.0	2.748121787267695	0.0
9	0.0	0.0	0.0	2.9712478111054623	0.0
10	0.0	0.0	0.0	3.9230638874766988	0.0
11	0.0	0.0	0.0	4.574083488674236	0.0
12	0.0	0.0	0.0	5.53719708523979	0.0
13	0.0	0.0	0.0	5.79280347963622	0.0
14	0.0	0.0	0.0	5.902954301530814	0.0
15	0.0	0.0	0.0	6.1105462351013955	0.0
16	0.0	0.0	0.0	6.503135061853923	0.0
17	0.0	0.0	0.0	6.950799299553748	0.0
18	0.0	0.0	0.0	7.49731683895385	0.0
19	0.0	0.0	0.0	7.75151104332599	0.0
20	0.0	0.0	0.0	8.0508953284754	0.0
21	0.0	0.0	0.0	8.375699034062023	0.0
22	0.0	0.0	0.0	8.748517200474495	0.0
23	0.0	0.0	0.0	9.050725865672485	0.0
24	0.0	0.0	0.0	9.265378749364514	0.0
25	0.0	0.0	0.0	9.501214483420888	0.0
26	0.0	0.0	0.0	9.758233067841608	0.0
27	0.0	0.0	0.0	9.981359091679376	0.0
28	0.0	0.0	0.0	10.210133875614302	0.0
29	0.0	0.0	0.0	10.43043551940349	0.0
30	0.0	0.0	0.0	10.752414844941535	0.0
31	0.0014121900242896684	0.0	0.0	11.044738179969496	0.0
32	0.0014121900242896684	0.0	0.0	11.318703044681692	0.0
33	0.0014121900242896684	0.0	0.0	11.57995819917528	0.0
34	0.0014121900242896684	0.0	0.0	11.869457154154663	0.0
35	0.0014121900242896684	0.0	0.0	12.219680280178501	0.0
36	0.0014121900242896684	0.0	0.0	12.513415805230752	0.0
37	0.0014121900242896684	0.0	0.0	12.808563520307292	0.0
38	0.0014121900242896684	0.0	0.0	13.093825905213805	0.0
39	0.0014121900242896684	0.0	0.0	13.407332090606111	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATG	25	3.853712E-5	45.0	45
TTAGGAT	20	6.988478E-4	45.0	5
TTAATCA	20	6.988478E-4	45.0	12
AGTCAAG	25	3.853712E-5	45.0	12
CCCTACA	25	3.853712E-5	45.0	27
TGGGTTT	20	6.988478E-4	45.0	31
GTGGGTT	20	6.988478E-4	45.0	30
CTGAGTG	20	6.988478E-4	45.0	26
GACGTAG	20	6.988478E-4	45.0	9
TCTTACG	25	3.853712E-5	45.0	1
ACCGATA	20	6.988478E-4	45.0	10
TCGGGGC	20	6.988478E-4	45.0	5
TCGGGCG	20	6.988478E-4	45.0	19
TGAGTGG	20	6.988478E-4	45.0	27
TATGACC	25	3.853712E-5	45.0	28
ATCAACG	20	6.988478E-4	45.0	15
TCGGATC	20	6.988478E-4	45.0	38
GTTAATC	40	6.661139E-9	45.0	40
TATCTAA	20	6.988478E-4	45.0	45
CGGATCA	20	6.988478E-4	45.0	39
>>END_MODULE