##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936550.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254377
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.130279073972886	33.0	31.0	34.0	30.0	34.0
2	32.25469283779587	33.0	31.0	34.0	30.0	34.0
3	32.099667029644976	33.0	31.0	34.0	30.0	34.0
4	35.851610011911454	37.0	35.0	37.0	35.0	37.0
5	35.93907468049391	37.0	35.0	37.0	35.0	37.0
6	35.9126493354352	37.0	35.0	37.0	35.0	37.0
7	26.72777806169583	36.0	10.0	37.0	0.0	37.0
8	31.156256265307004	37.0	19.0	37.0	17.0	37.0
9	36.04821583712364	39.0	32.0	39.0	32.0	39.0
10	36.763952715850884	38.0	35.0	39.0	33.0	39.0
11	37.119130267280454	39.0	37.0	39.0	34.0	39.0
12	37.24109884148331	39.0	37.0	39.0	34.0	39.0
13	37.25600191841244	39.0	37.0	39.0	34.0	39.0
14	38.50800976503379	40.0	38.0	41.0	34.0	41.0
15	38.59173588807164	40.0	38.0	41.0	35.0	41.0
16	38.520656348647876	40.0	38.0	41.0	34.0	41.0
17	38.443027474968254	40.0	38.0	41.0	34.0	41.0
18	38.32369278669062	40.0	37.0	41.0	34.0	41.0
19	38.21784595305393	40.0	37.0	41.0	34.0	41.0
20	38.271781646925625	40.0	37.0	41.0	34.0	41.0
21	38.219905887717836	40.0	37.0	41.0	34.0	41.0
22	38.19722301937675	40.0	37.0	41.0	34.0	41.0
23	38.14403031720635	40.0	37.0	41.0	34.0	41.0
24	38.114007948831855	40.0	37.0	41.0	34.0	41.0
25	38.0000864858065	40.0	37.0	41.0	34.0	41.0
26	37.87728450292283	40.0	37.0	41.0	33.0	41.0
27	37.774024381135085	40.0	36.0	41.0	33.0	41.0
28	37.75654245470306	40.0	36.0	41.0	33.0	41.0
29	37.733368189734136	40.0	36.0	41.0	33.0	41.0
30	37.661305857054685	40.0	36.0	41.0	33.0	41.0
31	37.55749930221679	40.0	36.0	41.0	33.0	41.0
32	37.4252113988293	40.0	36.0	41.0	33.0	41.0
33	37.307492422664	40.0	36.0	41.0	32.0	41.0
34	37.17162715182583	40.0	36.0	41.0	32.0	41.0
35	37.08140279978143	40.0	36.0	41.0	31.0	41.0
36	36.90381992082617	40.0	35.0	41.0	31.0	41.0
37	36.819394048990276	39.0	35.0	41.0	31.0	41.0
38	36.68585210140854	39.0	35.0	41.0	30.0	41.0
39	36.62873608856147	39.0	35.0	41.0	30.0	41.0
40	36.47003856480735	39.0	35.0	41.0	30.0	41.0
41	36.347633630398974	39.0	35.0	41.0	30.0	41.0
42	36.28513977285682	39.0	35.0	41.0	30.0	41.0
43	36.251941016679964	39.0	35.0	41.0	30.0	41.0
44	36.08445732122008	39.0	35.0	40.0	29.0	41.0
45	35.99166198201881	39.0	35.0	40.0	29.0	41.0
46	35.83478459137422	39.0	35.0	40.0	28.0	41.0
47	35.703774319219114	38.0	35.0	40.0	27.0	41.0
48	35.614568140987586	38.0	35.0	40.0	27.0	41.0
49	35.43814888924706	38.0	35.0	40.0	27.0	41.0
50	35.25716554562716	38.0	34.0	40.0	26.0	41.0
51	33.1983394725152	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	18.0
10	24.0
11	15.0
12	9.0
13	6.0
14	12.0
15	9.0
16	22.0
17	38.0
18	63.0
19	151.0
20	237.0
21	380.0
22	595.0
23	967.0
24	1355.0
25	1900.0
26	2350.0
27	2787.0
28	3179.0
29	3597.0
30	4343.0
31	5405.0
32	7118.0
33	10198.0
34	16323.0
35	20387.0
36	23151.0
37	38980.0
38	51555.0
39	59183.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.54790330886833	20.02264355661086	24.182217731949034	17.24723540257177
2	30.564084017029842	26.033800225649333	27.217476422789794	16.18463933453103
3	32.14166375104667	24.4157294094985	27.54494313558223	15.897663703872599
4	29.248320406326044	25.739748483550006	27.033497525326585	17.97843358479737
5	25.737389779736375	30.946587152140324	24.664965779138836	18.65105728898446
6	27.028387000397046	34.75825251496794	24.4003978347099	13.812962649925112
7	61.280697547341155	28.58670398660256	6.305994645742342	3.826603820313944
8	84.60670579494214	5.083792952979239	7.116602523026846	3.1928987290517616
9	78.06759258895262	8.051435467829245	8.941059922870386	4.939912020347752
10	40.75918813414735	28.59299386343891	17.031414003624544	13.616403998789197
11	29.93037892576766	27.81501472224297	24.911057210360998	17.34354914162837
12	28.32292227677817	24.445606324471157	27.461602267500602	19.769869131250072
13	24.45032373209842	26.0161099470471	29.173235001592126	20.360331319262354
14	20.21566415202632	28.11221140276047	29.013629376869766	22.65849506834344
15	20.30568801424657	27.67742366644783	32.158960912346636	19.85792740695896
16	23.125518423442372	26.647063217193377	31.2135138003829	19.013904558981352
17	23.470282297534762	26.609323956175285	28.364985828121252	21.555407918168704
18	23.54890575798913	26.7402320178318	28.87289338265645	20.83796884152262
19	24.473124535630188	28.069361616812845	26.75792229643403	20.69959155112294
20	26.186329738930798	26.681657539793296	28.17511017112396	18.95690255015194
21	24.911057210360998	27.15418453712403	28.016683898308415	19.918074354206553
22	24.151161465069563	25.968149636169933	27.667202616588764	22.213486282171736
23	22.678544050759307	27.270547258596494	29.077314379837798	20.9735943108064
24	22.717069546381946	25.545941653529997	30.411947621050643	21.325041179037413
25	22.178891959571818	28.390538452768922	27.873589200281472	21.556980387377788
26	22.006706581176758	29.82895466178153	27.458850446384698	20.705488310657017
27	22.225279801239893	29.22591272009655	28.182579399867123	20.366228078796432
28	21.41703062776902	28.37599311258486	29.246354819814684	20.96062143983143
29	21.489757328689308	27.656588449427428	29.21333296642385	21.640321255459416
30	22.22213486282172	27.55595042004584	28.824539954477018	21.39737476265543
31	22.729256182752373	27.28037519115329	27.863761267724673	22.12660735836966
32	23.282765344351102	28.249016223951063	27.6192423057116	20.848976125986233
33	22.96787838523137	27.95142642613129	28.10552840862185	20.97516678001549
34	22.208375757242205	27.753688423088562	28.64606469924561	21.391871120423623
35	22.883358165242925	27.13138373359227	28.480955432291445	21.504302668873365
36	22.760312449631844	28.07761708016055	27.535901437629974	21.62616903257763
37	22.826749273715784	28.286755484969163	28.333143326637234	20.55335191467782
38	22.57829913867999	27.79811067824528	27.758405830715827	21.8651843523589
39	23.13023583106963	26.903375698274605	28.43928499825063	21.52710347240513
40	24.382707556107665	26.58495068343443	28.564296300373073	20.468045460084834
41	21.530248410823305	27.070450551740134	28.915350051301807	22.48395098613475
42	22.266164000676163	27.701403821886412	28.18375875177394	21.848673425663485
43	23.068123297310684	27.091678886062816	27.77177181899307	22.068425997633433
44	21.93044182453602	27.904645467160943	27.700224469979595	22.464688238323433
45	22.206017053428575	27.342094607609962	27.707693698722764	22.744194640238703
46	22.336138880480547	28.16213730014899	27.287451302594178	22.214272516776283
47	22.44503237320984	27.127059443267278	28.70739099840001	21.72051718512287
48	22.33495952857373	26.066822079040165	29.13353015406267	22.464688238323433
49	21.784202188090905	26.491781882796005	28.883114432515516	22.84090149659757
50	20.97398742810867	27.333839144262257	29.04586499565605	22.64630843197302
51	21.40405775679405	26.433993639362047	27.86494061963149	24.29700798421241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	266.0
2	449.0
3	1186.5
4	1924.0
5	1395.0
6	866.0
7	733.0
8	600.0
9	571.5
10	543.0
11	570.5
12	598.0
13	625.5
14	653.0
15	627.0
16	601.0
17	621.5
18	642.0
19	688.5
20	735.0
21	874.0
22	1013.0
23	1114.0
24	1215.0
25	1652.0
26	2511.0
27	2933.0
28	3586.5
29	4240.0
30	4767.5
31	5295.0
32	6180.0
33	7065.0
34	8044.0
35	9023.0
36	9390.0
37	9757.0
38	10590.5
39	11424.0
40	12773.0
41	14122.0
42	15096.0
43	16070.0
44	17023.0
45	17976.0
46	19137.5
47	20299.0
48	20368.0
49	20437.0
50	19954.5
51	19472.0
52	17995.5
53	16519.0
54	15223.0
55	13927.0
56	12843.5
57	11760.0
58	11250.0
59	10740.0
60	10159.5
61	9579.0
62	8593.5
63	7608.0
64	6295.0
65	4982.0
66	4089.5
67	3197.0
68	2618.0
69	2039.0
70	1741.5
71	1444.0
72	1211.0
73	978.0
74	808.0
75	521.0
76	404.0
77	303.5
78	203.0
79	154.0
80	105.0
81	78.0
82	51.0
83	44.0
84	37.0
85	29.0
86	21.0
87	17.5
88	14.0
89	7.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.04241181980595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.088071502414884	25.682898593822785
2	12.987933849601305	10.920881271866714
3	7.352147290620094	9.273060124563614
4	4.95688515402013	8.335976279551861
5	3.519588662316537	7.398599798871588
6	2.686513192522138	6.776849639961441
7	1.937733632426613	5.70268967600977
8	1.4386214096456857	4.838649100569094
9	1.0856748620828538	4.107995068868853
>10	2.9209810553278146	15.54876261236977
>50	0.012924694510959466	0.38204168535176786
>100	0.011930487240885662	0.6600041807116427
>500	9.94207270073805E-4	0.37159196748109585
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	889	0.34948128171965237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06918864519984118	0.0
2	0.0	0.0	0.0	0.2767545807993647	0.0
3	0.0	0.0	0.0	0.39547600608545586	0.0
4	0.0	0.0	0.0	0.525204715835158	0.0
5	0.0	0.0	0.0	0.9745377923318539	0.0
6	0.0	0.0	0.0	1.3452474083741848	0.0
7	0.0	0.0	0.0	1.6593481328893729	0.0
8	0.0	0.0	0.0	2.1625382797973085	0.0
9	0.0	0.0	0.0	2.3712835673036476	0.0
10	0.0	0.0	0.0	2.8811567083502045	0.0
11	0.0	0.0	0.0	3.4940265825919794	0.0
12	0.0	0.0	0.0	4.0306317001930205	0.0
13	0.0	0.0	0.0	4.3195729173628115	0.0
14	0.0	0.0	0.0	4.423355885162573	0.0
15	0.0	0.0	0.0	4.647825864759786	0.0
16	0.0	0.0	0.0	4.983548040899924	0.0
17	0.0	0.0	0.0	5.377844695078564	0.0
18	0.0	0.0	0.0	5.7792174606980975	0.0
19	0.0	0.0	0.0	6.030026299547522	0.0
20	0.0	0.0	0.0	6.288304367140111	0.0
21	0.0	0.0	0.0	6.616164197234813	0.0
22	0.0	0.0	0.0	6.951100138770408	0.0
23	0.0	0.0	0.0	7.308443766535497	0.0
24	0.0	0.0	0.0	7.557287018873562	0.0
25	0.0	0.0	0.0	7.803378450095724	0.0
26	0.0	0.0	0.0	8.04593182559744	0.0
27	0.0	0.0	0.0	8.281409089658263	0.0
28	0.0	0.0	0.0	8.514920767207727	0.0
29	0.0	0.0	0.0	8.777523125125306	0.0
30	0.0	0.0	0.0	9.071574867224632	0.0
31	0.0	0.0	0.0	9.36012296709215	0.0
32	0.0	0.0	0.0	9.648277949657398	0.0
33	0.0	0.0	0.0	9.892796911670473	0.0
34	0.0	0.0	0.0	10.154219917681237	0.0
35	0.0	0.0	0.0	10.430974498480602	0.0
36	0.0	0.0	0.0	10.692790621793637	0.0
37	0.0	0.0	0.0	10.991559771520224	0.0
38	0.0	0.0	0.0	11.274604229155937	0.0
39	0.0	0.0	0.0	11.578483903812058	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATA	20	7.022918E-4	45.000004	1
TTGACGG	20	7.022918E-4	45.000004	2
TTACGCG	20	7.022918E-4	45.000004	1
AGTACGG	35	1.2069358E-7	45.000004	2
CACAACG	55	1.8189894E-12	45.0	12
ACAACGA	60	3.6379788E-12	41.249996	13
ACGAGCG	60	3.6379788E-12	41.249996	16
GCGATGC	55	6.002665E-11	40.909092	9
AGGCGAT	90	0.0	40.0	7
CACGACC	40	3.4440745E-7	39.375004	27
CGTTTTT	410	0.0	38.963413	1
GCGATTG	35	6.22912E-6	38.57143	9
TACGAAT	65	9.094947E-12	38.07692	12
TGCGACG	30	1.13742426E-4	37.499996	1
CGAATAT	60	1.546141E-10	37.499996	14
GCGATTC	30	1.13742426E-4	37.499996	9
TGGGCCG	30	1.13742426E-4	37.499996	6
ATGGGCG	55	2.732122E-9	36.81818	5
CGATGCG	55	2.732122E-9	36.81818	10
CGATTGG	25	0.0021040316	36.0	10
>>END_MODULE