##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58252
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919384742154776	31.0	31.0	34.0	30.0	34.0
2	32.03646226738996	33.0	31.0	34.0	30.0	34.0
3	31.964722241296435	33.0	31.0	34.0	30.0	34.0
4	35.70620751218842	37.0	35.0	37.0	35.0	37.0
5	35.777707203186154	37.0	35.0	37.0	35.0	37.0
6	35.73832658106159	37.0	35.0	37.0	35.0	37.0
7	26.989459589370323	35.0	17.0	37.0	0.0	37.0
8	31.17190826066058	35.0	23.0	37.0	17.0	37.0
9	35.897531415230375	39.0	32.0	39.0	32.0	39.0
10	36.687993545286	38.0	35.0	39.0	33.0	39.0
11	36.96558058092426	39.0	37.0	39.0	33.0	39.0
12	36.82575705555174	39.0	35.0	39.0	33.0	39.0
13	36.50312435624528	39.0	35.0	39.0	32.0	39.0
14	37.66686122364897	39.0	36.0	41.0	33.0	41.0
15	37.778496875643754	40.0	36.0	41.0	33.0	41.0
16	37.822340863833	40.0	36.0	41.0	33.0	41.0
17	37.67932431504498	39.0	36.0	41.0	33.0	41.0
18	37.59937856210946	39.0	36.0	41.0	33.0	41.0
19	37.455126004257366	39.0	36.0	41.0	32.0	41.0
20	37.57045251665179	39.0	35.0	41.0	33.0	41.0
21	37.56564581473597	39.0	35.0	41.0	33.0	41.0
22	37.54492549612031	39.0	35.0	41.0	33.0	41.0
23	37.549079859918976	39.0	35.0	41.0	33.0	41.0
24	37.48865275012017	39.0	35.0	41.0	33.0	41.0
25	37.447572615532515	39.0	35.0	41.0	33.0	41.0
26	37.26023140836366	39.0	35.0	41.0	33.0	41.0
27	37.22172629265948	39.0	35.0	41.0	32.0	41.0
28	37.22167479228181	39.0	35.0	41.0	32.0	41.0
29	37.21089404655634	39.0	35.0	41.0	32.0	41.0
30	37.13290530797226	39.0	35.0	41.0	32.0	41.0
31	36.996652475451484	39.0	35.0	41.0	32.0	41.0
32	36.7820675684955	39.0	35.0	40.0	31.0	41.0
33	36.631566298152855	39.0	35.0	40.0	31.0	41.0
34	36.54025612854494	39.0	35.0	40.0	31.0	41.0
35	36.44403625626588	39.0	35.0	40.0	30.0	41.0
36	35.96523724507313	38.0	35.0	40.0	30.0	41.0
37	35.992120442216574	38.0	35.0	40.0	30.0	41.0
38	35.77520085147291	38.0	35.0	40.0	29.0	41.0
39	35.8198173453272	38.0	35.0	40.0	29.0	41.0
40	35.65436379866786	38.0	35.0	40.0	29.0	41.0
41	35.31741399436929	38.0	34.0	40.0	26.0	41.0
42	35.36192748746824	38.0	34.0	40.0	27.0	41.0
43	35.07299320195015	38.0	34.0	40.0	25.0	41.0
44	34.99495296298839	38.0	34.0	40.0	25.0	41.0
45	34.95802719219941	37.0	34.0	40.0	26.0	41.0
46	34.76390510197075	37.0	34.0	40.0	24.0	41.0
47	34.50932156835817	37.0	33.0	40.0	23.0	41.0
48	34.325293552152715	37.0	33.0	40.0	23.0	41.0
49	34.12876811096615	37.0	33.0	40.0	23.0	41.0
50	33.909119000206005	36.0	33.0	40.0	22.0	41.0
51	31.410320675684954	35.0	29.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	2.0
11	4.0
12	2.0
13	1.0
14	0.0
15	4.0
16	5.0
17	8.0
18	28.0
19	38.0
20	54.0
21	102.0
22	163.0
23	244.0
24	347.0
25	508.0
26	641.0
27	764.0
28	966.0
29	1152.0
30	1380.0
31	1741.0
32	2309.0
33	3149.0
34	5007.0
35	6437.0
36	5275.0
37	8110.0
38	10001.0
39	9799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61992721279956	17.796813843301518	22.22927968138433	15.353979262514592
2	26.625695255098535	29.834168783904413	26.557028084872623	16.983107876124425
3	29.90970267115292	27.75527020531484	26.618828538075945	15.716198585456292
4	27.81878733777381	22.991485270891985	31.578314907642657	17.611412483691545
5	28.934628853944925	26.725262651926112	24.438645883403147	19.90146261072581
6	23.884158483828884	36.6287852777587	24.301311542951314	15.1857446954611
7	58.19027672869601	27.075465220078282	10.209091533337912	4.525166517887798
8	76.95873103069422	7.807457254686534	9.798805191238069	5.435006523381172
9	72.10052873721075	5.907093318684337	10.909496669642243	11.082881274462679
10	45.289432122502234	21.3245897136579	17.894664560873448	15.491313602966422
11	35.9043466318753	22.306530247888485	23.939092220009613	17.850030900226603
12	32.9997253313191	20.536633935315525	28.510609077799902	17.953031655565475
13	22.09881205795509	28.656526814529975	32.268419968413106	16.976241159101836
14	18.8611549818032	32.48815491313603	27.830804092563344	20.819886012497424
15	15.599464396072237	24.483279544049992	42.18567602829088	17.731580031586898
16	18.088649316761657	23.29533749914166	34.64430405822976	23.971709125866923
17	18.225983657213487	22.356313946302272	31.70363249330495	27.71406990317929
18	21.269655977477168	24.078143239717093	33.04950902973288	21.602691753072854
19	23.599189727391334	26.920964087069972	26.5776282359404	22.902217949598295
20	25.35020256815217	25.818856004944035	29.928586142965045	18.90235528393875
21	21.98036118931539	29.106296779509716	28.61017647462748	20.303165556547416
22	20.358099292728145	24.347661882853807	27.492618279200716	27.801620545217332
23	18.816521321156355	29.08912998695324	28.67712696559775	23.41722172629266
24	22.91423470438783	22.28593009682071	31.391196868777037	23.40863833001442
25	17.865481013527432	28.568976172491933	30.017853464258735	23.5476893497219
26	18.11783286410767	28.433358511295747	30.223854974936483	23.224953649660097
27	21.951177641969373	28.568976172491933	26.948430955160337	22.531415230378357
28	16.675822289363456	25.77593902355284	35.42539311954954	22.12284556753416
29	21.053354391265536	24.406028977545834	32.689006386046835	21.8516102451418
30	23.98200920140081	27.751836846803545	29.010162741193433	19.25599121060221
31	26.8179633317311	24.333928448808624	27.477168165899883	21.370940053560393
32	27.73467005424706	27.99560530110554	25.67293826821397	18.59678637643343
33	26.53299457529355	24.665247545148663	25.556204078829914	23.245553800727873
34	24.50559637437341	25.099567396827577	29.73975142484378	20.65508480395523
35	24.86266565954817	24.680697658449496	27.935521527157864	22.521115154844466
36	27.859987639909363	22.924534779921718	31.245279132046967	17.970198448121952
37	24.315044976996496	27.40850099567397	29.973219803611894	18.30323422371764
38	23.504772368330702	27.30378356107945	27.008514729108015	22.182929341481838
39	25.868639703357825	25.848039552290047	28.22049028359541	20.06283046075671
40	29.082263269930646	22.1709125866923	27.94582160269175	20.8010025406853
41	22.323697040444962	24.385428826478062	28.321774359678635	24.969099773398337
42	25.17853464258738	21.329739751424846	30.512257089885324	22.979468516102454
43	25.46178671976928	23.37773810341276	28.37155805809243	22.78891711872554
44	22.380347455881346	25.640321362356655	29.853052255716545	22.126278926045458
45	21.317722996635307	26.787063105129437	26.58277827370734	25.31243562452791
46	26.36647668749571	26.424843782187736	25.803405891643205	21.40527363867335
47	20.677401634278652	24.263544599327062	32.8349241227769	22.224129643617385
48	24.20861086314633	22.873034402252284	29.338048479022177	23.58030625557921
49	21.84989356588615	20.797569182174	34.27521801826547	23.07731923367438
50	21.42072375197418	24.237794410492345	29.952619652544122	24.388862184989357
51	21.96147771750326	24.2721279956053	26.56732816040651	27.19906612648493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	57.5
2	95.0
3	164.0
4	233.0
5	181.5
6	130.0
7	131.5
8	133.0
9	131.0
10	129.0
11	132.0
12	135.0
13	136.5
14	138.0
15	119.0
16	100.0
17	110.0
18	120.0
19	125.5
20	131.0
21	142.5
22	154.0
23	154.5
24	155.0
25	227.5
26	329.0
27	358.0
28	398.0
29	438.0
30	592.5
31	747.0
32	912.0
33	1077.0
34	1309.5
35	1542.0
36	1623.5
37	1705.0
38	1935.5
39	2166.0
40	2545.5
41	2925.0
42	3535.5
43	4146.0
44	4691.5
45	5237.0
46	6680.0
47	8123.0
48	7033.0
49	5943.0
50	5575.0
51	5207.0
52	4457.0
53	3707.0
54	3303.0
55	2899.0
56	2586.0
57	2273.0
58	2139.5
59	2006.0
60	1845.5
61	1685.0
62	1467.0
63	1249.0
64	1124.5
65	1000.0
66	821.0
67	642.0
68	534.0
69	426.0
70	352.0
71	278.0
72	242.0
73	206.0
74	157.5
75	87.5
76	66.0
77	65.0
78	64.0
79	40.5
80	17.0
81	15.5
82	14.0
83	14.0
84	14.0
85	8.5
86	3.0
87	2.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	58252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.80168921238756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.12854391241173	36.64251871180389
2	10.556550535599635	10.725811989287921
3	5.355996350488291	8.162809860605645
4	3.3453857331125603	6.798049852365584
5	2.5816916162606023	6.557714756574881
6	1.8686851620315617	5.695941770239648
7	1.1252661102287704	4.001579344915196
8	0.9191362822289054	3.7354940602897755
9	0.6927313891798736	3.1672732266703285
>10	1.371946068326969	8.806564581473598
>50	0.016895887540972526	0.6523381171461924
>100	0.030412597573750547	2.798187186706036
>500	0.0067583550163890106	2.2557165419213074
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	751	1.289226120991554	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	563	0.9664904209297535	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	469	0.8051225708988533	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCT	195	0.33475245485133553	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	178	0.3055689075053217	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	173	0.29698551122708233	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	149	0.2557852090915333	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTT	138	0.2369017372794067	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	116	0.19913479365515346	No Hit
GAATCTATCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	108	0.18540135960997048	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	104	0.17853464258737897	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99	0.1699512463091396	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	89	0.15278445375266084	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	84	0.14420105747442147	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20256815216644922	0.0
2	0.0	0.0	0.0	1.3630433289844126	0.0
3	0.0	0.0	0.0	1.6531621231889033	0.0
4	0.0	0.0	0.0	2.1887660509510405	0.0
5	0.0	0.0	0.0	4.403282290736799	0.0
6	0.0	0.0	0.0	5.064203804161231	0.0
7	0.0	0.0	0.0	6.073611206482181	0.0
8	0.0	0.0	0.0	7.337087138639017	0.0
9	0.0	0.0	0.0	7.73020668818238	0.0
10	0.0	0.0	0.0	10.511227082331937	0.0
11	0.0	0.0	0.0	11.747236146398407	0.0
12	0.0	0.0	0.0	14.174620613884501	0.0
13	0.0	0.0	0.0	14.644990729932019	0.0
14	0.0	0.0	0.0	14.880175787955778	0.0
15	0.0	0.0	0.0	15.36427933804848	0.0
16	0.0	0.0	0.0	16.0749845498867	0.0
17	0.0	0.0	0.0	16.904140630364623	0.0
18	0.0	0.0	0.0	17.783080409256335	0.0
19	0.0	0.0	0.0	18.28606743116116	0.0
20	0.0	0.0	0.0	18.766737622742568	0.0
21	0.0	0.0	0.0	19.35384192817414	0.0
22	0.0017166792556478746	0.0	0.0	19.889445855936277	0.0
23	0.0017166792556478746	0.0	0.0	20.447366614021835	0.0
24	0.0017166792556478746	0.0	0.0	20.88683650346769	0.0
25	0.0017166792556478746	0.0	0.0	21.28338941152235	0.0
26	0.0017166792556478746	0.0	0.0	21.666208885531827	0.0
27	0.0017166792556478746	0.0	0.0	22.06791183135343	0.0
28	0.0017166792556478746	0.0	0.0	22.469614777175032	0.0
29	0.0017166792556478746	0.0	0.0	22.871317722996636	0.0
30	0.0017166792556478746	0.0	0.0	23.30907093318684	0.0
31	0.0017166792556478746	0.0	0.0	23.786307766256954	0.0
32	0.0017166792556478746	0.0	0.0	24.174277278033372	0.0
33	0.0017166792556478746	0.0	0.0	24.608597129712283	0.0
34	0.0017166792556478746	0.0	0.0	24.99141660372176	0.0
35	0.0017166792556478746	0.0	0.0	25.430886493167616	0.0
36	0.0017166792556478746	0.0	0.0	25.878939778891713	0.0
37	0.0017166792556478746	0.0	0.0	26.270342649179426	0.0
38	0.0017166792556478746	0.0	0.0	26.661745519467143	0.0
39	0.0017166792556478746	0.0	0.0	27.05143171049921	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAAGC	30	2.1300912E-6	45.000004	13
AGAAGGG	30	2.1300912E-6	45.000004	3
TGTCACT	30	2.1300912E-6	45.000004	3
GAATGAC	30	2.1300912E-6	45.000004	1
ACTTATA	30	2.1300912E-6	45.000004	7
GTCTTAC	30	2.1300912E-6	45.000004	31
CTTACAC	30	2.1300912E-6	45.000004	33
CGAGATA	30	2.1300912E-6	45.000004	19
CAAGCGA	30	2.1300912E-6	45.000004	15
ACCGGCG	20	6.9782155E-4	45.0	20
CACACCG	20	6.9782155E-4	45.0	17
ATCTATC	25	3.8452323E-5	45.0	3
TACGGCT	85	0.0	45.0	7
ATGAAAC	35	1.1855809E-7	45.0	3
TACACGC	25	3.8452323E-5	45.0	35
CGGCGCA	20	6.9782155E-4	45.0	22
GCTGTCA	20	6.9782155E-4	45.0	1
ATACGGC	95	0.0	45.0	6
TGGGCGA	20	6.9782155E-4	45.0	6
TTGCGGG	20	6.9782155E-4	45.0	3
>>END_MODULE