##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99956
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938032734403137	33.0	31.0	34.0	30.0	34.0
2	32.054463964144226	33.0	31.0	34.0	30.0	34.0
3	31.95063828084357	33.0	31.0	34.0	30.0	34.0
4	35.70046820601065	37.0	35.0	37.0	35.0	37.0
5	35.78981751970867	37.0	35.0	37.0	35.0	37.0
6	35.756022649965985	37.0	35.0	37.0	35.0	37.0
7	26.86151906839009	35.0	11.0	37.0	0.0	37.0
8	31.188803073352275	35.0	19.0	37.0	17.0	37.0
9	35.96103285445596	39.0	32.0	39.0	32.0	39.0
10	36.53718636199928	37.0	35.0	39.0	32.0	39.0
11	36.90741926447637	39.0	37.0	39.0	33.0	39.0
12	36.940803953739646	39.0	35.0	39.0	33.0	39.0
13	36.85440593861299	39.0	35.0	39.0	33.0	39.0
14	38.03442514706471	40.0	37.0	41.0	33.0	41.0
15	38.130587458481735	40.0	37.0	41.0	33.0	41.0
16	38.11298971547481	40.0	37.0	41.0	33.0	41.0
17	38.036245948217214	40.0	36.0	41.0	33.0	41.0
18	37.93914322301813	40.0	36.0	41.0	33.0	41.0
19	37.79110808755853	40.0	36.0	41.0	33.0	41.0
20	37.86404017767818	40.0	36.0	41.0	33.0	41.0
21	37.81856016647325	40.0	36.0	41.0	33.0	41.0
22	37.81499859938373	40.0	36.0	41.0	33.0	41.0
23	37.77276001440634	39.0	36.0	41.0	33.0	41.0
24	37.749319700668295	39.0	36.0	41.0	33.0	41.0
25	37.65624874944976	39.0	36.0	41.0	33.0	41.0
26	37.524420745127856	39.0	35.0	41.0	33.0	41.0
27	37.44662651566689	39.0	35.0	41.0	33.0	41.0
28	37.45774140621874	39.0	36.0	41.0	33.0	41.0
29	37.465044619632636	39.0	36.0	41.0	33.0	41.0
30	37.41404217855857	39.0	36.0	41.0	33.0	41.0
31	37.29157829444956	39.0	35.0	41.0	32.0	41.0
32	37.14939573412301	39.0	35.0	41.0	32.0	41.0
33	37.095492016487256	39.0	35.0	41.0	32.0	41.0
34	36.99007563327864	39.0	35.0	41.0	31.0	41.0
35	36.90073632398255	39.0	35.0	41.0	31.0	41.0
36	36.75282124134619	39.0	35.0	41.0	31.0	41.0
37	36.67620753131378	39.0	35.0	41.0	31.0	41.0
38	36.512725599263675	39.0	35.0	41.0	30.0	41.0
39	36.52955300332146	39.0	35.0	41.0	30.0	41.0
40	36.330585457601345	39.0	35.0	40.0	30.0	41.0
41	36.2691384209052	39.0	35.0	40.0	30.0	41.0
42	36.19327504101805	39.0	35.0	40.0	30.0	41.0
43	36.14348313257833	39.0	35.0	40.0	30.0	41.0
44	35.91428228420505	38.0	35.0	40.0	29.0	41.0
45	35.792918884309096	38.0	35.0	40.0	28.0	41.0
46	35.62733602785225	38.0	35.0	40.0	27.0	41.0
47	35.53531553883709	38.0	35.0	40.0	27.0	41.0
48	35.45112849653848	38.0	34.0	40.0	27.0	41.0
49	35.28408499739886	38.0	34.0	40.0	26.0	41.0
50	35.049671855616474	38.0	34.0	40.0	26.0	41.0
51	32.83905918604186	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	4.0
12	3.0
13	1.0
14	3.0
15	2.0
16	5.0
17	19.0
18	26.0
19	61.0
20	83.0
21	132.0
22	225.0
23	359.0
24	492.0
25	750.0
26	948.0
27	1197.0
28	1360.0
29	1668.0
30	2027.0
31	2506.0
32	3380.0
33	4587.0
34	7236.0
35	9548.0
36	9017.0
37	14512.0
38	18715.0
39	21077.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07819440553843	19.60262515506823	25.006002641162112	16.31317779823122
2	27.753211413021727	27.18496138300852	28.13137780623474	16.930449397735003
3	30.43439113209812	25.178078354475968	28.238424906959064	16.149105606466847
4	27.541118091960463	25.91040057625355	28.823682420264916	17.72479891152107
5	25.083036536075877	30.806554884149023	25.94341510264516	18.166993477129935
6	25.309136019848733	36.122894073392295	24.658849893953338	13.909120012805634
7	61.78318460122454	27.799231661931252	6.46084277081916	3.956740966025051
8	84.84133018528152	4.8401296570491015	6.581896034255072	3.7366441234143024
9	79.02577133938932	6.933050542238585	8.702829244867742	5.3383488735043425
10	42.6007443275041	26.71475449197647	17.38564968586178	13.298851494657649
11	31.755972627956304	25.835367561727157	24.481771979671056	17.926887830645484
12	27.69218456120693	23.900516227139942	28.987754612029292	19.419544599623833
13	22.817039497378847	29.014766497258794	31.049661851214534	17.118532154147825
14	19.862739605426388	31.50886390011605	28.690623874504784	19.937772619952778
15	18.854295890191686	26.98087158349674	36.66213133778863	17.502701188522952
16	21.498459322101724	25.049021569490577	33.66981471847613	19.782704389931567
17	21.469446556484854	24.424746888630995	29.606026651726758	24.49977990315739
18	23.25423186201929	26.21353395494017	30.971627516107088	19.56060666693345
19	24.240665892992915	28.246428428508548	27.580135259514186	19.93277041898435
20	24.975989435351558	27.008883908919923	28.879707071111287	19.13541958461723
21	22.642962903677617	27.96030253311457	28.544559606226738	20.85217495698107
22	20.849173636400014	25.697306814998598	29.47196766577294	23.981551882828445
23	20.46500460202489	28.63660010404578	28.576573692424667	22.321821601504663
24	21.05126255552443	25.022009684261075	32.124134619232464	21.802593140982033
25	19.072391852415063	28.769658649805912	30.39937572531914	21.758573772459883
26	18.916323182200166	30.599463764056186	29.152827243987357	21.33138580975629
27	20.310936812197365	29.67905878586578	29.058785865780944	20.951218536155906
28	17.97190763936132	27.965304734082995	32.776421625515226	21.286366001040456
29	19.580615470807157	27.327023890511825	30.965624874944975	22.126735763736043
30	20.7351234543199	27.716195125855375	29.139821521469443	22.408859898355278
31	22.536916243146983	27.416063067749807	27.713193805274322	22.333826883828884
32	23.068149985993838	27.79422946096282	27.714194245468004	21.423426307575333
33	21.895634078994757	27.049901956861017	28.038336868222018	23.016127095922208
34	19.41154107807435	26.887830645484012	30.175277121933654	23.525351154507984
35	19.499579815118654	26.91284165032614	29.59102004882148	23.996558485733726
36	22.33882908479731	27.013886109888354	29.790107647364838	20.857177157949497
37	19.538596982672377	27.928288446916643	30.357357237184363	22.17575733322662
38	20.328944735683702	28.71563487934691	27.82424266677338	23.131177718196007
39	20.816159110008403	27.469086398015126	28.298451318580177	23.416303173396294
40	21.314378326463643	25.633278642602747	29.419944775701307	23.6323982552323
41	18.385089439353315	26.256552883268636	29.82512305414382	25.533234623234225
42	21.019248469326506	25.313137780623475	29.86814198247229	23.799471767577735
43	21.155308335667694	25.76033454720077	29.515987034295087	23.56837008283645
44	19.66365200688303	27.67317619752691	29.27888270839169	23.38428908719837
45	19.965784945375965	28.271439433350675	27.107927488094763	24.654848133178596
46	21.87362439473368	27.918284044979792	28.296450438192807	21.911641122093723
47	19.46456440833967	26.594701668734245	30.051222537916683	23.889511385009403
48	20.39797510904798	24.639841530273323	30.4493977350034	24.5127856256753
49	20.18087958701829	23.67841850414182	32.01108487734603	24.129617031493858
50	19.266477249989997	25.373164192244584	30.604465965024612	24.755892592740807
51	19.32450278122374	25.708311657129133	28.48353275441194	26.483652807235185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	113.0
2	177.0
3	339.0
4	501.0
5	381.0
6	261.0
7	226.5
8	192.0
9	195.0
10	198.0
11	189.0
12	180.0
13	185.0
14	190.0
15	192.0
16	194.0
17	220.5
18	247.0
19	263.0
20	279.0
21	295.0
22	311.0
23	385.0
24	459.0
25	585.5
26	830.0
27	948.0
28	1224.0
29	1500.0
30	1768.5
31	2037.0
32	2371.0
33	2705.0
34	2923.5
35	3142.0
36	3470.0
37	3798.0
38	4234.0
39	4670.0
40	5457.5
41	6245.0
42	6897.5
43	7550.0
44	8021.5
45	8493.0
46	9487.0
47	10481.0
48	10109.5
49	9738.0
50	9327.0
51	8916.0
52	7746.5
53	6577.0
54	5835.5
55	5094.0
56	4524.5
57	3955.0
58	3486.0
59	3017.0
60	2715.0
61	2413.0
62	2123.0
63	1833.0
64	1534.5
65	1236.0
66	979.5
67	723.0
68	560.5
69	398.0
70	296.5
71	195.0
72	148.5
73	102.0
74	94.0
75	74.5
76	63.0
77	50.0
78	37.0
79	34.0
80	31.0
81	21.5
82	12.0
83	9.5
84	7.0
85	4.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	99956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.922285805754534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20174294410103	38.21081275761335
2	10.675060020038186	11.298971547480892
3	5.41976218832114	8.60478610588659
4	3.338437399572771	7.067109528192404
5	2.3837879733076237	6.307775421185322
6	1.6200684322955066	5.144263475929408
7	1.2533318210174105	4.643042938893113
8	0.7958562543715383	3.3694825723318256
9	0.6767613754513317	3.2234183040537836
>10	1.6200684322955066	10.921805594461564
>50	0.00567118471048602	0.19808715834967386
>100	0.0094519745174767	1.0104445956220736
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG	250	0.25011004842130535	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218	0.2180959622233783	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	201	0.20108847893072954	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	191	0.1910840769938773	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	150	0.15006602905278324	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11304974188643004	0.0
2	0.0	0.0	0.0	0.6422826043459122	0.0
3	0.0	0.0	0.0	0.8143583176597703	0.0
4	0.0	0.0	0.0	1.0964824522790027	0.0
5	0.0	0.0	0.0	1.9618632198167194	0.0
6	0.0	0.0	0.0	2.394053383488735	0.0
7	0.0	0.0	0.0	2.8782664372323823	0.0
8	0.0	0.0	0.0	3.890711913241826	0.0
9	0.0	0.0	0.0	4.282884469166433	0.0
10	0.0	0.0	0.0	5.519428548561367	0.0
11	0.0	0.0	0.0	6.515866981471848	0.0
12	0.0	0.0	0.0	7.641362199367721	0.0
13	0.0	0.0	0.0	7.992516707351235	0.0
14	0.0	0.0	0.0	8.141582296210332	0.0
15	0.0	0.0	0.0	8.433710832766417	0.0
16	0.0	0.0	0.0	9.010964824522791	0.0
17	0.0	0.0	0.0	9.700268117971907	0.0
18	0.0	0.0	0.0	10.549641842410661	0.0
19	0.0	0.0	0.0	11.036856216735364	0.0
20	0.0	0.0	0.0	11.482052102925287	0.0
21	0.0	0.0	0.0	12.053303453519549	0.0
22	0.0	0.0	0.0	12.66857417263596	0.0
23	0.0	0.0	0.0	13.328864700468205	0.0
24	0.0	0.0	0.0	13.82208171595502	0.0
25	0.0	0.0	0.0	14.220256913041739	0.0
26	0.0	0.0	0.0	14.61342990916003	0.0
27	0.0	0.0	0.0	14.96958661811197	0.0
28	0.0	0.0	0.0	15.4007763415903	0.0
29	0.0	0.0	0.0	15.811957261194927	0.0
30	0.0	0.0	0.0	16.301172515907	0.0
31	0.0	0.0	0.0	16.800392172555924	0.0
32	0.0	0.0	0.0	17.21557485293529	0.0
33	0.0	0.0	0.0	17.641762375445197	0.0
34	0.0	0.0	0.0	18.083956941054065	0.0
35	0.0	0.0	0.0	18.58717835847773	0.0
36	0.0	0.0	0.0	19.000360158469725	0.0
37	0.0	0.0	0.0	19.415542838849095	0.0
38	0.0	0.0	0.0	19.88074752891272	0.0
39	0.0	0.0	0.0	20.344951778782665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	20	7.0023816E-4	45.000004	2
AGCGCGG	20	7.0023816E-4	45.000004	2
TGGGCAC	20	7.0023816E-4	45.000004	6
CGTTTTA	20	7.0023816E-4	45.000004	1
CTAGGCA	20	7.0023816E-4	45.000004	5
TATGGGC	45	3.765308E-10	45.0	4
GTAGGGC	25	3.865212E-5	45.0	4
TTAACGG	25	3.865212E-5	45.0	2
GGGTTTA	25	3.865212E-5	45.0	8
CGTTTTT	115	0.0	41.086956	1
GCGGGAG	50	1.0604708E-9	40.5	5
TAACGGG	45	1.8981154E-8	40.0	3
GGCACAC	40	3.4161712E-7	39.375004	8
GGCGATT	40	3.4161712E-7	39.375004	8
CGCGGGA	35	6.191236E-6	38.571426	4
GGATAAC	35	6.191236E-6	38.571426	8
AGCGGGA	35	6.191236E-6	38.571426	4
CTGGGCA	35	6.191236E-6	38.571426	5
TGGGAAC	35	6.191236E-6	38.571426	6
AAAGGAG	35	6.191236E-6	38.571426	5
>>END_MODULE