##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55509
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870741681529122	31.0	31.0	34.0	30.0	34.0
2	31.977571204669513	33.0	31.0	34.0	30.0	34.0
3	31.81267902502297	33.0	31.0	34.0	30.0	34.0
4	35.61054964059882	37.0	35.0	37.0	33.0	37.0
5	35.73251184492605	37.0	35.0	37.0	35.0	37.0
6	35.71599200129709	37.0	35.0	37.0	35.0	37.0
7	26.909510169522058	35.0	15.0	37.0	0.0	37.0
8	31.176349781116574	35.0	19.0	37.0	17.0	37.0
9	35.89135095209786	39.0	32.0	39.0	32.0	39.0
10	36.67976364193194	38.0	35.0	39.0	33.0	39.0
11	36.97036516600912	39.0	37.0	39.0	33.0	39.0
12	36.75292294943162	39.0	35.0	39.0	33.0	39.0
13	36.487704696535694	39.0	35.0	39.0	33.0	39.0
14	37.5276621809076	39.0	36.0	41.0	33.0	41.0
15	37.76319155452269	40.0	36.0	41.0	33.0	41.0
16	37.77131636311229	40.0	36.0	41.0	33.0	41.0
17	37.688771190257434	39.0	35.0	41.0	33.0	41.0
18	37.56752958979625	39.0	36.0	41.0	33.0	41.0
19	37.50970112954656	39.0	36.0	41.0	33.0	41.0
20	37.529896052892326	39.0	35.0	41.0	33.0	41.0
21	37.46657298816408	39.0	35.0	41.0	33.0	41.0
22	37.47084256607037	39.0	35.0	41.0	33.0	41.0
23	37.43654182204688	39.0	35.0	41.0	33.0	41.0
24	37.39487290349313	39.0	35.0	41.0	33.0	41.0
25	37.34434055738709	39.0	35.0	41.0	33.0	41.0
26	37.17035075393179	39.0	35.0	41.0	33.0	41.0
27	37.07719468914951	39.0	35.0	41.0	32.0	41.0
28	37.09596641985984	39.0	35.0	41.0	32.0	41.0
29	37.10230773388099	39.0	35.0	41.0	33.0	41.0
30	37.02940063773442	39.0	35.0	41.0	32.0	41.0
31	36.78371074960817	39.0	35.0	40.0	32.0	41.0
32	36.672971950494514	38.0	35.0	40.0	31.0	41.0
33	36.565980291484266	38.0	35.0	40.0	31.0	41.0
34	36.39181033706246	38.0	35.0	40.0	30.0	41.0
35	36.34486299518997	38.0	35.0	40.0	30.0	41.0
36	36.05287430867067	38.0	35.0	40.0	30.0	41.0
37	35.97312147579672	38.0	35.0	40.0	30.0	41.0
38	35.881730890486224	38.0	35.0	40.0	30.0	41.0
39	35.93793799203733	38.0	35.0	40.0	30.0	41.0
40	35.79855518924859	38.0	35.0	40.0	29.0	41.0
41	35.76097569763462	38.0	35.0	40.0	29.0	41.0
42	35.6455529733917	38.0	35.0	40.0	29.0	41.0
43	35.60323551135852	38.0	35.0	40.0	29.0	41.0
44	35.37246212325929	38.0	34.0	40.0	28.0	41.0
45	35.27872957538417	38.0	34.0	40.0	27.0	41.0
46	35.16267632275847	38.0	34.0	40.0	27.0	41.0
47	34.988488353240015	37.0	34.0	40.0	26.0	41.0
48	34.86757102451855	37.0	34.0	40.0	26.0	41.0
49	34.715757805040624	37.0	34.0	40.0	24.0	41.0
50	34.447476985714026	37.0	34.0	40.0	24.0	41.0
51	32.11281053522852	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	3.0
12	3.0
13	0.0
14	1.0
15	2.0
16	1.0
17	11.0
18	20.0
19	32.0
20	61.0
21	84.0
22	120.0
23	238.0
24	341.0
25	462.0
26	628.0
27	696.0
28	810.0
29	1013.0
30	1177.0
31	1574.0
32	2096.0
33	2996.0
34	5012.0
35	6438.0
36	5059.0
37	7648.0
38	9503.0
39	9464.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.901475436415716	18.791547316651354	24.075375164387758	16.231602082545173
2	29.771748725431912	26.660541533805326	26.557855482894666	17.009854257868092
3	29.597002287917274	24.98153452593273	29.43666792772343	15.984795258426562
4	27.301878974580703	25.04098434488101	29.917671008304957	17.739465672233333
5	26.002990506044064	27.71082166855825	27.415374083481957	18.870813741915725
6	24.73833072114432	34.72770181412023	26.864112125961555	13.669855338773893
7	58.260822569313085	27.797294132483017	9.394872903493127	4.547010394710767
8	78.03058963411338	6.105316255021708	10.587472301788898	5.276621809076006
9	72.50175647192347	7.377182078581851	11.808895854726261	8.312165594768416
10	45.976328163000595	22.66839611594516	18.328559332720822	13.026716388333423
11	37.055252301428595	20.479561872849448	25.0698084995226	17.395377326199355
12	32.771262317822334	21.011007224053756	27.88736961573799	18.33036084238592
13	22.2774685186186	31.074240213298744	30.825631879515036	15.82265938856762
14	16.51984362896107	33.92963303248122	30.389666540560988	19.160856797996722
15	15.096650993532581	22.944027094705362	44.03069772469329	17.928624187068763
16	17.723252085247438	23.003476913653643	37.896557315029995	21.376713686068925
17	18.537534453872347	22.302689653929995	30.238339728692644	28.921436163505014
18	20.603866039741302	24.54556918697869	33.4270118359185	21.42355293736151
19	23.662829451080007	25.804824442883135	28.49267686321137	22.03966924282549
20	26.112882595615126	25.332828910627104	28.759300293646074	19.794988200111693
21	21.481201246644687	27.476625412095334	30.6346718550145	20.407501486245476
22	20.454340737538057	23.70786719270749	28.54852366282945	27.289268406925004
23	18.656434091768904	27.35051973553838	29.05294636905727	24.940099803635444
24	21.926174133924228	22.05588282981138	33.72786394999009	22.290079086274297
25	17.872777387450682	26.62811435983354	32.054261471112795	23.444846781602983
26	16.842313859013856	30.49415410113675	29.83660307337549	22.826928966473904
27	20.052604082220903	29.94649516294655	28.52510403718316	21.47579671764939
28	16.004611864742653	26.658740024140233	35.77257741987786	21.56407069123926
29	19.032949611774665	24.687888450521537	33.76029112396188	22.518870813741916
30	21.347889531427334	26.89834080959844	31.339062134068353	20.41470752490587
31	24.35460916247816	25.936334648435388	29.10519014934515	20.603866039741302
32	27.90718622205408	27.77207299717163	25.439117980867966	18.88162279990632
33	25.215730782395646	26.105676556954727	25.370660613594193	23.30793204705543
34	19.948116521645137	27.202795943000236	30.632870345349406	22.216217190005224
35	22.958439172026157	26.07685240231314	28.14858851717739	22.81611990848331
36	24.107802338359548	27.595525049991892	29.144823361977334	19.151849249671223
37	24.29335783386478	26.145309769586916	31.737195770055305	17.824136626493
38	20.8921075861572	28.065719072582823	26.864112125961555	24.17806121529842
39	21.508223891621178	27.195589904339833	30.191500477400062	21.104685726638923
40	23.72948530868868	24.138428002666235	28.701651984362897	23.430434704282188
41	20.076023707867193	26.1795384532238	28.121565872200904	25.6228719667081
42	23.56374641949954	24.001513268118682	29.49431623700661	22.940424075375166
43	24.949107351960944	24.338395575492264	26.91635590624944	23.796141166297357
44	20.53180565313733	25.981372390062873	28.9628708858023	24.523951070997498
45	20.793024554576736	28.422417986272496	26.70557927543281	24.078978183717954
46	25.599452341061813	27.152353672377455	26.70557927543281	20.542614711127925
47	20.659712839359383	25.529193464122933	31.025599452341062	22.785494244176622
48	22.81792141814841	22.481039110774827	30.600443171377616	24.10059629969915
49	21.066854023671837	21.380316705399125	33.75308508530148	23.799744185627556
50	20.020176908249113	24.58700390927597	31.54083121655948	23.851987965915438
51	19.97153614729143	25.750779152930154	26.961393647876918	27.31629105190149
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	80.5
2	143.0
3	317.5
4	492.0
5	358.5
6	225.0
7	208.0
8	191.0
9	174.5
10	158.0
11	166.5
12	175.0
13	164.5
14	154.0
15	147.0
16	140.0
17	155.0
18	170.0
19	181.0
20	192.0
21	186.0
22	180.0
23	226.5
24	273.0
25	300.5
26	368.5
27	409.0
28	485.0
29	561.0
30	677.0
31	793.0
32	903.5
33	1014.0
34	1190.5
35	1367.0
36	1519.0
37	1671.0
38	1854.5
39	2038.0
40	2552.5
41	3067.0
42	3638.5
43	4210.0
44	4761.0
45	5312.0
46	6023.0
47	6734.0
48	6307.0
49	5880.0
50	5368.5
51	4857.0
52	4157.5
53	3458.0
54	3037.5
55	2617.0
56	2296.5
57	1976.0
58	1811.0
59	1646.0
60	1545.0
61	1444.0
62	1350.0
63	1256.0
64	1041.0
65	826.0
66	708.5
67	591.0
68	456.5
69	322.0
70	290.0
71	258.0
72	191.0
73	124.0
74	114.0
75	84.5
76	65.0
77	48.5
78	32.0
79	25.0
80	18.0
81	15.5
82	13.0
83	7.5
84	2.0
85	2.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	55509.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31239979102487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.52747252747253	37.21558666162244
2	10.392163746796335	10.664937217388172
3	5.073201558824562	7.809544398205697
4	3.163290383737668	6.492640833018069
5	2.3838781027279428	6.116125313012304
6	1.6711722782010323	5.145111603523753
7	1.3271073973949374	4.766794573852889
8	1.035705508548959	4.251562809634474
9	0.7127058245269108	3.2913581581365183
>10	1.667661412070358	10.933362157487975
>50	0.02457606291472106	0.9566016321677566
>100	0.021065196784046624	2.356374641949954
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	356	0.6413374407753697	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	245	0.4413698679493415	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.4341638292889441	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	230	0.41434722297285126	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	135	0.2432038047884127	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	101	0.18195247617503468	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	100	0.1801509665099353	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	85	0.15312832153344502	Illumina Single End Adapter 1 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	81	0.14592228287304762	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	81	0.14592228287304762	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	66	0.11889963789655732	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	63	0.11349510890125926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20176908249112757	0.0
2	0.0	0.0	0.0	1.0394710767623268	0.0
3	0.0	0.0	0.0	1.4267956547586877	0.0
4	0.0	0.0	0.0	1.9041957160100165	0.0
5	0.0	0.0	0.0	3.6336449945053957	0.0
6	0.0	0.0	0.0	4.255165828964673	0.0
7	0.0	0.0	0.0	5.154119151849249	0.0
8	0.0	0.0	0.0	6.523266497324758	0.0
9	0.0	0.0	0.0	7.0186816552270805	0.0
10	0.0	0.0	0.0	8.9102668035814	0.0
11	0.0	0.0	0.0	10.347871516330684	0.0
12	0.0	0.0	0.0	12.098938910807256	0.0
13	0.0	0.0	0.0	12.574537462393486	0.0
14	0.0	0.0	0.0	12.805130699526202	0.0
15	0.0	0.0	0.0	13.28613378010773	0.0
16	0.0	0.0	0.0	14.086004071411843	0.0
17	0.0	0.0	0.0	14.907492478697149	0.0
18	0.0	0.0	0.0	15.916337891152786	0.0
19	0.0	0.0	0.0	16.509034570970474	0.0
20	0.0	0.0	0.0	17.072907096146572	0.0
21	0.0	0.0	0.0	17.789907942856114	0.0
22	0.0	0.0	0.0	18.49249671224486	0.0
23	0.0	0.0	0.0	19.294168513214075	0.0
24	0.0	0.0	0.0	19.753553477814407	0.0
25	0.0	0.0	0.0	20.202129384424147	0.0
26	0.0	0.0	0.0	20.708353600317064	0.0
27	0.0	0.0	0.0	21.129906861950314	0.0
28	0.0	0.0	0.0	21.574879749229854	0.0
29	0.0	0.0	0.0	21.987425462537608	0.0
30	0.0	0.0	0.0	22.545893458718407	0.0
31	0.0	0.0	0.0	23.075537300257615	0.0
32	0.0	0.0	0.0	23.56374641949954	0.0
33	0.0	0.0	0.0	23.97629213280729	0.0
34	0.0	0.0	0.0	24.500531445351204	0.0
35	0.0	0.0	0.0	25.022969248230016	0.0
36	0.0	0.0	0.0	25.507575348141742	0.0
37	0.0	0.0	0.0	25.912915022789097	0.0
38	0.0	0.0	0.0	26.341674323082742	0.0
39	0.0	0.0	0.0	26.806463816678377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGAT	20	6.975348E-4	45.000004	5
TTCGTGT	20	6.975348E-4	45.000004	25
GCGATGA	20	6.975348E-4	45.000004	9
AACTGGG	20	6.975348E-4	45.000004	3
TGCCGGC	20	6.975348E-4	45.000004	24
AGGATCA	20	6.975348E-4	45.000004	7
TATGCGG	20	6.975348E-4	45.000004	2
GTCCGGG	20	6.975348E-4	45.000004	3
GCGGCTA	20	6.975348E-4	45.000004	24
TGTCACT	20	6.975348E-4	45.000004	3
CGTAGCC	20	6.975348E-4	45.000004	16
GAGTTCT	20	6.975348E-4	45.000004	9
CCGTAGC	20	6.975348E-4	45.000004	15
CGGCGCA	20	6.975348E-4	45.000004	22
TGCTTGG	20	6.975348E-4	45.000004	2
TTCTTCT	20	6.975348E-4	45.000004	36
CTGGAAC	20	6.975348E-4	45.000004	35
TGGATAG	20	6.975348E-4	45.000004	1
CTTCGTG	20	6.975348E-4	45.000004	24
TCACTTA	20	6.975348E-4	45.000004	5
>>END_MODULE