##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936544.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114152
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945134557432194	33.0	31.0	34.0	30.0	34.0
2	32.06246933912678	33.0	31.0	34.0	30.0	34.0
3	31.985256500105123	33.0	31.0	34.0	30.0	34.0
4	35.73531782185157	37.0	35.0	37.0	35.0	37.0
5	35.79311794799916	37.0	35.0	37.0	35.0	37.0
6	35.763955077440606	37.0	35.0	37.0	35.0	37.0
7	26.758155792276963	35.0	10.0	37.0	0.0	37.0
8	31.103922839722475	35.0	19.0	37.0	17.0	37.0
9	35.90258602565001	39.0	32.0	39.0	32.0	39.0
10	36.650448524773985	38.0	35.0	39.0	33.0	39.0
11	36.934236807064266	39.0	37.0	39.0	33.0	39.0
12	36.852363515312916	39.0	35.0	39.0	33.0	39.0
13	36.65994463522321	39.0	35.0	39.0	33.0	39.0
14	37.74007463732567	40.0	36.0	41.0	33.0	41.0
15	37.879791856472075	40.0	36.0	41.0	33.0	41.0
16	37.88957705515453	40.0	36.0	41.0	33.0	41.0
17	37.78691569135889	40.0	36.0	41.0	33.0	41.0
18	37.67271707898241	39.0	36.0	41.0	33.0	41.0
19	37.55972738103581	39.0	36.0	41.0	33.0	41.0
20	37.645201135328335	39.0	36.0	41.0	33.0	41.0
21	37.55305207092298	39.0	35.0	41.0	33.0	41.0
22	37.57615284883313	39.0	35.0	41.0	33.0	41.0
23	37.5532447964118	39.0	35.0	41.0	33.0	41.0
24	37.47938713294555	39.0	35.0	41.0	33.0	41.0
25	37.40651937767188	39.0	35.0	41.0	33.0	41.0
26	37.261248160347606	39.0	35.0	41.0	32.0	41.0
27	37.12440430303455	39.0	35.0	41.0	32.0	41.0
28	37.104194407456724	39.0	35.0	41.0	32.0	41.0
29	37.08026140584484	39.0	35.0	41.0	32.0	41.0
30	36.98080629336324	39.0	35.0	41.0	32.0	41.0
31	36.846327703412996	39.0	35.0	41.0	31.0	41.0
32	36.67983040156984	39.0	35.0	41.0	31.0	41.0
33	36.566087322166936	39.0	35.0	40.0	31.0	41.0
34	36.4109345434158	39.0	35.0	40.0	30.0	41.0
35	36.27800651762562	38.0	35.0	40.0	30.0	41.0
36	36.1034673067489	38.0	35.0	40.0	30.0	41.0
37	35.92216518326442	38.0	35.0	40.0	29.0	41.0
38	35.76448069241012	38.0	35.0	40.0	29.0	41.0
39	35.601443689116266	38.0	35.0	40.0	28.0	41.0
40	35.32567979536057	38.0	34.0	40.0	26.0	41.0
41	35.18700504590371	38.0	34.0	40.0	25.0	41.0
42	35.067690447823956	38.0	34.0	40.0	25.0	41.0
43	34.980744971616794	38.0	34.0	40.0	25.0	41.0
44	34.71144614198612	37.0	34.0	40.0	24.0	41.0
45	34.55212348447684	37.0	33.0	40.0	24.0	41.0
46	34.300914570046956	37.0	33.0	40.0	23.0	41.0
47	34.095355315719395	37.0	33.0	40.0	22.0	41.0
48	33.96480131754152	36.0	33.0	40.0	22.0	41.0
49	33.78806328404233	36.0	33.0	40.0	21.0	41.0
50	33.58626217674679	36.0	33.0	40.0	20.0	41.0
51	31.26673207652954	35.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	10.0
11	3.0
12	6.0
13	3.0
14	4.0
15	9.0
16	12.0
17	39.0
18	46.0
19	90.0
20	154.0
21	268.0
22	329.0
23	531.0
24	775.0
25	1013.0
26	1461.0
27	1699.0
28	2011.0
29	2400.0
30	2800.0
31	3436.0
32	4377.0
33	6156.0
34	9600.0
35	11617.0
36	10730.0
37	16177.0
38	19372.0
39	19006.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3216413203448	18.781098885696267	23.673698226925502	15.223561567033428
2	29.046359240311165	26.042469689536755	27.37490363725559	17.536267432896487
3	31.12078631999439	25.685051510267016	26.250087602494922	16.944074567243675
4	28.480447123134066	24.722300091106593	28.306118158245148	18.491134627514192
5	26.001296516924803	28.728362183754992	25.612341439484197	19.65799985983601
6	26.6416707547831	34.85790875324129	24.392038685261756	14.108381806713854
7	61.051930758988014	27.83481673558063	7.142231410750578	3.9710210946807765
8	82.46986474174784	5.209720372836219	7.82553087111921	4.494884014296727
9	77.66924802018362	6.170719742098255	8.955603055575024	7.204429182143107
10	49.56811970004906	19.162169738594155	15.998843647067067	15.27086691428972
11	35.17940990959422	22.998282991099586	21.937416777629824	19.88489032167636
12	31.35643703132665	20.465694862989697	27.944319854229448	20.2335482514542
13	24.774861588058027	24.533954727030626	31.08486929707758	19.606314387833766
14	19.51520779311795	27.824304436190346	31.27233863620436	21.38814913448735
15	18.606769920807345	22.774896629055995	38.23936505711683	20.378968393019832
16	22.01976312285374	22.436750998668444	33.06205760740066	22.48142827107716
17	23.578211507463735	22.14591071553718	27.79276753801948	26.483110238979606
18	24.494533604317052	23.97242273459948	30.10196930408578	21.431074356997687
19	25.896173523021936	24.814282710771604	26.286004625411728	23.00353914079473
20	26.847536617842877	25.12439554278506	26.736281449295674	21.29178639007639
21	24.975471301422665	25.621977713925293	28.78530380545238	20.617247179199662
22	24.5549793258112	22.689046184035323	27.868981708599062	24.88699278155442
23	24.232602144509073	24.443724157264	27.959212278365687	23.364461419861236
24	24.38327843576985	22.579543065386503	28.863270025930337	24.173908472913308
25	22.38594155161539	24.879108557011705	28.586446141986123	24.148503749386784
26	21.048251454201417	25.911941972107368	27.386291961595067	25.653514612096153
27	21.756955638096574	26.178253556661296	29.055995514752258	23.00879529048987
28	20.147697806433527	24.44109608241643	30.76950031536898	24.641705795781064
29	21.187539421122715	23.836638867474946	29.6455603055575	25.33026140584484
30	22.06444039526246	24.694267292732498	29.797112621767468	23.44417969023758
31	25.692059709860537	24.204569346134978	25.660522811689678	24.442848132314808
32	26.653935104071763	25.685051510267016	25.222510337094402	22.438503048566822
33	25.09198261966501	24.829175134907842	25.673663185927538	24.405179059499616
34	23.600988156142687	24.49278155441867	27.127864601583852	24.77836568785479
35	22.20986053682809	25.430128250052565	29.08140023827879	23.278610974840564
36	25.71045623379354	24.712663816665497	26.185261756254818	23.391618193286146
37	23.326792347046045	26.168617282220197	27.77174293923891	22.73284743149485
38	22.393825776158106	28.13704534305137	24.494533604317052	24.974595276473472
39	24.023232181652535	26.625902305697668	26.51815123694723	22.832714275702575
40	25.181337164482443	24.583012124185295	27.119980377041138	23.11567033429112
41	21.280398065736915	23.315404022706566	27.546604527296935	27.857593384259584
42	22.3018431564931	22.98163851706497	28.88429462471091	25.832223701731028
43	23.726259723876936	22.897540121942672	27.294309341930056	26.08189081225033
44	22.167811339266944	24.817786810568364	27.503679304786598	25.510722545378094
45	21.57649449856332	25.994288317331275	26.637290630037143	25.79192655406826
46	24.48051720513	25.280327983740975	26.131824234354195	24.107330576774828
47	21.699137991449994	24.22384189501717	29.486999789754016	24.590020323778823
48	22.95448174364006	23.16560375639498	28.889550774406054	24.990363725558904
49	22.627724437591983	22.73284743149485	29.666584904338077	24.972843226575094
50	22.053928095872173	24.136239400098113	28.653900063073795	25.15593244095592
51	21.332959562688345	25.419615950662276	25.98815614268694	27.259268343962432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	129.0
2	227.0
3	335.5
4	444.0
5	326.5
6	209.0
7	217.5
8	226.0
9	198.5
10	171.0
11	196.5
12	222.0
13	213.5
14	205.0
15	203.5
16	202.0
17	201.0
18	200.0
19	212.5
20	225.0
21	262.0
22	299.0
23	318.0
24	337.0
25	431.5
26	608.5
27	691.0
28	889.0
29	1087.0
30	1297.0
31	1507.0
32	1718.0
33	1929.0
34	2181.0
35	2433.0
36	2735.5
37	3038.0
38	3374.0
39	3710.0
40	4471.0
41	5232.0
42	5995.5
43	6759.0
44	7574.5
45	8390.0
46	9380.5
47	10371.0
48	10128.5
49	9886.0
50	9598.5
51	9311.0
52	8453.0
53	7595.0
54	7011.5
55	6428.0
56	6331.5
57	6235.0
58	5911.0
59	5587.0
60	5316.0
61	5045.0
62	4642.5
63	4240.0
64	3800.0
65	3360.0
66	3033.5
67	2707.0
68	2291.0
69	1875.0
70	1589.0
71	1303.0
72	1018.0
73	733.0
74	635.0
75	433.5
76	330.0
77	234.0
78	138.0
79	115.0
80	92.0
81	70.0
82	48.0
83	33.0
84	18.0
85	10.5
86	3.0
87	3.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	114152.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.253626743289644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49124928856004	34.71949681126919
2	9.929923164484917	9.781694582661714
3	5.595475241889584	8.26792347046044
4	3.731502561183836	7.351601373607121
5	2.6981360273192943	6.644649239610343
6	1.9173306772908367	5.666129371364496
7	1.543824701195219	5.3227275912818
8	1.2058907228229938	4.751559324409559
9	0.8750711439954468	3.8790384750157685
>10	1.9902532726237907	12.065491625201485
>50	0.01245019920318725	0.41523582591632213
>100	0.008892999430848036	1.1344523092017662
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	368	0.3223771813021235	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	338	0.2960964328264069	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	273	0.23915481112902096	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.18308921438082557	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1173873431915341	0.0
2	0.0	0.0	0.0	0.6491344873501997	0.0
3	0.0	0.0	0.0	0.8173312775947859	0.0
4	0.0	0.0	0.0	1.1528488331347677	0.0
5	0.0	0.0	0.0	2.3039456163711542	0.0
6	0.0	0.0	0.0	2.739330016118859	0.0
7	0.0	0.0	0.0	3.262316910785619	0.0
8	0.0	0.0	0.0	4.217184105403322	0.0
9	0.0	0.0	0.0	4.536933211857874	0.0
10	0.0	0.0	0.0	5.884259583712944	0.0
11	0.0	0.0	0.0	6.903076599621557	0.0
12	0.0	0.0	0.0	8.200469549372766	0.0
13	0.0	0.0	0.0	8.55438362884575	0.0
14	0.0	0.0	0.0	8.690167495970286	0.0
15	0.0	0.0	0.0	8.973123554558834	0.0
16	0.0	0.0	0.0	9.5048706987175	0.0
17	0.0	0.0	0.0	10.030485668231831	0.0
18	0.0	0.0	0.0	10.707652953956128	0.0
19	0.0	0.0	0.0	11.108872380685401	0.0
20	0.0	0.0	0.0	11.528488331347678	0.0
21	0.0	0.0	0.0	12.034830751979817	0.0
22	0.0	0.0	0.0	12.54818137220548	0.0
23	0.0	0.0	0.0	12.995830121241854	0.0
24	0.0	0.0	0.0	13.36989277454622	0.0
25	0.0	0.0	0.0	13.716798654425679	0.0
26	0.0	0.0	0.0	14.082977083187329	0.0
27	0.0	0.0	0.0	14.426378863270026	0.0
28	0.0	0.0	0.0	14.738243745181864	0.0
29	0.0	0.0	0.0	15.09828999929918	0.0
30	0.0	0.0	0.0	15.526666199453361	0.0
31	0.0	0.0	0.0	15.893720653164202	0.0
32	0.0	0.0	0.0	16.260775106875045	0.0
33	0.0	0.0	0.0	16.59979676221179	0.0
34	0.0	0.0	0.0	16.963347116125867	0.0
35	0.0	0.0	0.0	17.375078842245426	0.0
36	0.0	0.0	0.0	17.745637395753032	0.0
37	0.0	0.0	0.0	18.117071974209825	0.0
38	0.0	0.0	0.0	18.522671525685052	0.0
39	0.0	0.0	0.0	19.077195318522673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATA	20	7.006586E-4	45.000004	8
CGCGGGT	20	7.006586E-4	45.000004	4
GCACGGG	20	7.006586E-4	45.000004	3
AATGCGG	20	7.006586E-4	45.000004	2
ATCCCCC	20	7.006586E-4	45.000004	42
GGTATCA	20	7.006586E-4	45.000004	8
GCGATGT	20	7.006586E-4	45.000004	9
CCCATCC	20	7.006586E-4	45.000004	39
AATCTAT	55	6.002665E-11	40.909092	2
GGCGATT	45	1.9022991E-8	40.0	8
GGGATAT	45	1.9022991E-8	40.0	7
ATATGGG	40	3.4218647E-7	39.375004	3
AGGCGAT	40	3.4218647E-7	39.375004	7
CGTTTTA	40	3.4218647E-7	39.375004	1
ATCTCTT	70	0.0	38.571426	7
TATGGGC	35	6.1989867E-6	38.571426	4
TATCTCT	70	0.0	38.571426	6
TAGGGCA	35	6.1989867E-6	38.571426	5
ACGTAGG	35	6.1989867E-6	38.571426	2
ACCGGGG	30	1.1334929E-4	37.500004	4
>>END_MODULE