##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269387
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.901283283900113	33.0	31.0	34.0	30.0	34.0
2	32.00936199593893	33.0	31.0	34.0	30.0	34.0
3	31.433027577425786	33.0	31.0	34.0	28.0	34.0
4	35.431657800858986	37.0	35.0	37.0	33.0	37.0
5	35.69018920734854	37.0	35.0	37.0	35.0	37.0
6	35.71102168998504	37.0	35.0	37.0	35.0	37.0
7	26.99333301161526	35.0	16.0	37.0	0.0	37.0
8	31.263735815017057	36.0	23.0	37.0	17.0	37.0
9	36.038903139349706	39.0	32.0	39.0	32.0	39.0
10	36.71199055633716	38.0	35.0	39.0	33.0	39.0
11	37.083890462420236	39.0	37.0	39.0	34.0	39.0
12	37.15650718111861	39.0	37.0	39.0	34.0	39.0
13	37.15492210091801	39.0	37.0	39.0	34.0	39.0
14	38.320757868791	40.0	38.0	41.0	34.0	41.0
15	38.39952930171092	40.0	38.0	41.0	34.0	41.0
16	38.345436119783066	40.0	38.0	41.0	34.0	41.0
17	38.261683006232666	40.0	37.0	41.0	33.0	41.0
18	38.07874916013022	40.0	37.0	41.0	33.0	41.0
19	37.859016953305094	40.0	37.0	41.0	33.0	41.0
20	37.77825581783828	40.0	35.0	41.0	33.0	41.0
21	37.734382134252954	40.0	35.0	41.0	33.0	41.0
22	37.76001440307068	39.0	35.0	41.0	33.0	41.0
23	37.68848162680456	39.0	35.0	41.0	33.0	41.0
24	37.609866103412564	39.0	35.0	41.0	33.0	41.0
25	37.50683589037333	39.0	35.0	41.0	33.0	41.0
26	37.40869455467413	39.0	35.0	41.0	33.0	41.0
27	37.302457059917515	39.0	35.0	41.0	33.0	41.0
28	37.2602983811394	39.0	35.0	41.0	33.0	41.0
29	37.22031129935743	39.0	35.0	41.0	33.0	41.0
30	37.0985905036249	39.0	35.0	41.0	32.0	41.0
31	36.86847917679769	39.0	35.0	41.0	31.0	41.0
32	36.62560554147008	39.0	35.0	41.0	31.0	41.0
33	36.35237780590749	39.0	35.0	41.0	30.0	41.0
34	35.97968721578992	39.0	35.0	41.0	28.0	41.0
35	35.67348832720213	39.0	35.0	41.0	26.0	41.0
36	35.384610244740834	39.0	35.0	41.0	24.0	41.0
37	35.19903707305846	39.0	35.0	40.0	23.0	41.0
38	34.974828035502824	38.0	34.0	40.0	23.0	41.0
39	34.899185929536316	38.0	34.0	40.0	22.0	41.0
40	34.68856700583176	38.0	34.0	40.0	21.0	41.0
41	34.53846696388467	38.0	34.0	40.0	20.0	41.0
42	34.39096912620134	38.0	34.0	40.0	20.0	41.0
43	34.34790839944021	38.0	34.0	40.0	20.0	41.0
44	34.11760775390052	38.0	33.0	40.0	18.0	41.0
45	34.03382494329719	38.0	33.0	40.0	18.0	41.0
46	33.89104893703111	38.0	33.0	40.0	18.0	41.0
47	33.691128376647725	38.0	33.0	40.0	18.0	41.0
48	33.56334938211569	37.0	33.0	40.0	15.0	41.0
49	33.40992327023947	37.0	32.0	40.0	15.0	41.0
50	33.20975770916934	37.0	32.0	40.0	15.0	41.0
51	31.215841892890154	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	11.0
10	26.0
11	9.0
12	10.0
13	9.0
14	14.0
15	17.0
16	33.0
17	62.0
18	124.0
19	203.0
20	372.0
21	620.0
22	1147.0
23	1735.0
24	2779.0
25	4271.0
26	5579.0
27	6184.0
28	5691.0
29	5861.0
30	6232.0
31	7252.0
32	8849.0
33	11790.0
34	17628.0
35	20775.0
36	23776.0
37	38076.0
38	47723.0
39	52507.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.592062720175804	17.84607275035544	22.388979423654444	21.172885105814313
2	35.765645706734176	23.622149546934338	24.98041850571854	15.631786240612946
3	29.701878709811535	23.143655781459387	32.376098326942284	14.778367181786797
4	26.057307887908475	24.039764353884934	32.89542554020795	17.007502217998642
5	24.110666067776098	27.90335094121097	30.870086529787997	17.11589646122493
6	23.737225627071833	33.27554781782343	29.837742727006127	13.14948382809861
7	57.54472190565989	27.083341066940868	11.881419667615734	3.4905173597835084
8	79.28927528054435	4.53251270477046	13.12052920148337	3.057682813201825
9	74.06036668436116	5.923448421787243	14.809549087372442	5.206635806479155
10	38.12433413639114	23.556444817307444	23.30513350681362	15.0140875394878
11	28.913421954288808	25.17122207084974	28.841035387750708	17.074320587110737
12	25.54948828265655	22.947284018902174	32.14334767453515	19.35988002390613
13	23.17186798175116	22.3347823020413	34.97273439327065	19.52061532293689
14	20.083374476125428	25.359427143848812	35.02247695694298	19.534721423082775
15	20.482057411827594	24.769569429853703	36.6933073979071	18.0550657604116
16	23.873089644266425	24.562061272444478	33.478230204130114	18.08661887915898
17	24.506379298184395	24.16077984461017	32.503424441416996	18.82941641578844
18	24.17117381313872	24.468515555687542	32.88874370329675	18.471566927876996
19	23.450277853051556	25.884322554540496	31.85825596632354	18.807143626084407
20	23.793278814493647	26.648279241388785	32.14520374034382	17.413238203773755
21	23.462527887388777	26.149739965180206	32.83380415536013	17.553927992070886
22	23.295481964608538	23.232004513952045	33.680912590436805	19.79160093100261
23	21.8184247940695	25.070252090858137	33.741420335799425	19.36990277927294
24	21.96765248508651	25.016426182406725	33.07472149732541	19.941199835181354
25	21.782417117381314	26.99499233444821	31.697891880454517	19.524698667715963
26	21.327680994257335	26.573665395880276	31.562027863259917	20.53662574660247
27	21.52739367527015	25.985663747693838	32.91101649300078	19.575926084035235
28	20.463496753740902	26.250709945171817	32.98822883064142	20.297564470445863
29	21.81285659664349	25.324161893484092	32.99268338858223	19.87029812129019
30	23.317383541150836	24.811145303967898	32.63186419537691	19.239606959504357
31	23.04491308043818	25.43329856303385	31.697891880454517	19.823896476073454
32	23.91652158418929	25.677556823454733	31.474792770252463	18.931128822103517
33	23.316641114827366	26.13080809393178	31.318512029162505	19.23403876207835
34	22.54785865687654	26.16421727848783	32.21313574894111	19.074788315694523
35	22.438350774165052	26.898476912397406	31.535300515615084	19.12787179782246
36	24.308522682980247	27.1735458652422	29.741969731278793	18.775961720498763
37	22.696715134731818	27.786790008426536	30.614691874515103	18.90180298232654
38	22.34517627056985	27.92822222304714	29.821038134728106	19.905563371654907
39	23.012246322205602	26.562900214189995	29.856674598254557	20.56817886534985
40	23.329633575488053	25.903625638950658	30.726798249358726	20.039942536202563
41	22.340721712629044	26.606332154112856	30.090910103308623	20.962036029949477
42	22.37710060247896	26.5662411326456	30.795472684279495	20.261185580595946
43	22.702654545319557	25.973784926518356	30.249418123368983	21.074142404793104
44	22.43389621622424	26.611529138377133	29.770553144732297	21.18402150066633
45	22.097948304855098	25.700200826320497	29.731575762750246	22.470275106074162
46	22.427585592474767	26.570695690586405	29.13021044074139	21.871508276197442
47	21.30132485977423	26.594453332937373	30.92205637243074	21.18216543485766
48	21.995493472216552	26.428149836480603	30.22825897315015	21.348097718152694
49	22.15771362389425	25.704284171099573	30.602813053339617	21.53518915166656
50	20.94978599561226	26.412930096849514	30.847071313760498	21.790212593777724
51	21.124256181627178	26.142686915107262	30.04525088441536	22.687806018850203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	186.0
2	309.0
3	3948.0
4	7587.0
5	5270.5
6	2954.0
7	2561.5
8	2169.0
9	2102.5
10	2036.0
11	1938.5
12	1841.0
13	1794.5
14	1748.0
15	1658.0
16	1568.0
17	1514.0
18	1460.0
19	1411.0
20	1362.0
21	1388.5
22	1415.0
23	1504.0
24	1593.0
25	1738.0
26	2145.5
27	2408.0
28	2929.0
29	3450.0
30	4143.0
31	4836.0
32	5420.5
33	6005.0
34	6869.0
35	7733.0
36	8620.5
37	9508.0
38	10329.0
39	11150.0
40	11988.5
41	12827.0
42	13747.0
43	14667.0
44	15880.5
45	17094.0
46	19591.5
47	22089.0
48	21616.5
49	21144.0
50	20918.5
51	20693.0
52	19215.0
53	17737.0
54	16371.5
55	15006.0
56	13986.0
57	12966.0
58	11819.0
59	10672.0
60	9997.0
61	9322.0
62	8351.5
63	7381.0
64	6349.0
65	5317.0
66	4431.0
67	3545.0
68	2872.0
69	2199.0
70	1801.5
71	1404.0
72	1145.5
73	887.0
74	726.0
75	470.0
76	375.0
77	291.0
78	207.0
79	149.0
80	91.0
81	75.0
82	59.0
83	45.5
84	32.0
85	22.5
86	13.0
87	12.5
88	12.0
89	7.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269387.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.49644617436685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26447188800512	40.82685846339728
2	11.885016273003664	13.429223642984514
3	5.982536787223391	10.139762027566077
4	3.5817530839302956	8.094252812645616
5	2.2463843267881547	6.345636560266415
6	1.4548156971161033	4.931515003544635
7	0.9494227091865345	3.754730629039502
8	0.5938438356789989	2.684005303873449
9	0.40411313670737964	2.0547860468708725
>10	0.6153864462767545	4.395352930827412
>50	0.013091656519758656	0.52550858424426
>100	0.00720041108586726	0.9001322266365438
>500	0.0013091656519758654	0.48150047620410025
>1k	6.545828259879327E-4	1.4367352918993315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3885	1.4421631333360556	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.2531673763024942	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	620	0.23015216027499472	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	444	0.16481864380983494	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	440	0.16333379116289945	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	350	0.12992460660685184	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06013653220088572	0.0
2	0.0	0.0	0.0	0.24685675255301853	0.0
3	0.0	0.0	0.0	0.3890313934970878	0.0
4	0.0	0.0	0.0	0.6091607984052683	0.0
5	0.0	0.0	0.0	1.3267158400368244	0.0
6	0.0	0.0	0.0	1.8972704696217708	0.0
7	0.0	0.0	0.0	2.349779313775349	0.0
8	0.0	0.0	0.0	3.05434189474622	0.0
9	0.0	0.0	0.0	3.523926544339556	0.0
10	0.0	0.0	0.0	4.371406192577964	0.0
11	0.0	0.0	0.0	4.980566990983232	0.0
12	0.0	0.0	0.0	5.680675014013297	0.0
13	0.0	0.0	0.0	5.97504705126825	0.0
14	0.0	0.0	0.0	6.171047600663729	0.0
15	0.0	0.0	0.0	6.348116278810782	0.0
16	0.0	0.0	0.0	6.6061094262158155	0.0
17	0.0	0.0	0.0	6.872640476340729	0.0
18	0.0	0.0	0.0	7.168126153080884	0.0
19	0.0	0.0	0.0	7.406445002914023	0.0
20	0.0	0.0	0.0	7.641422934291558	0.0
21	0.0	0.0	0.0	7.915378247651149	0.0
22	0.0	0.0	0.0	8.195644184760216	0.0
23	0.0	0.0	0.0	8.491129861500369	0.0
24	0.0	0.0	0.0	8.734645695597782	0.0
25	0.0	0.0	0.0	8.979275169180399	0.0
26	0.0	0.0	0.0	9.204972771514587	0.0
27	0.0	0.0	0.0	9.467791690022162	0.0
28	0.0	0.0	0.0	9.703140834561431	0.0
29	0.0	0.0	0.0	9.967073392554207	0.0
30	0.0	0.0	0.0	10.28260458002799	0.0
31	0.0	0.0	0.0	10.559900811843185	0.0
32	0.0	0.0	0.0	10.858727407038945	0.0
33	0.0	0.0	0.0	11.116349341282245	0.0
34	0.0	0.0	0.0	11.403668328464255	0.0
35	0.0	0.0	0.0	11.680222133955981	0.0
36	0.0	0.0	0.0	11.950465315698233	0.0
37	0.0	0.0	0.0	12.254117681996533	0.0
38	0.0	0.0	0.0	12.601944414541162	0.0
39	3.712131617338624E-4	0.0	0.0	13.135377727952722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGATC	40	6.7811925E-9	45.0	6
CGAAGGA	50	2.1827873E-11	45.0	4
CGTTTTT	1750	0.0	43.585716	1
CGTTTTA	300	0.0	42.000004	1
CCGGGAT	55	6.002665E-11	40.90909	5
AGGCACC	50	1.0750227E-9	40.5	7
CGGTTTT	125	0.0	39.600002	1
CACGGGC	40	3.4450932E-7	39.375	4
AATACGG	35	6.2304916E-6	38.57143	2
CGGGCTC	65	9.094947E-12	38.07692	6
CACGGGT	30	1.1376027E-4	37.500004	4
TAACGTC	30	1.1376027E-4	37.500004	32
TACCTGG	30	1.1376027E-4	37.500004	10
TGGGCGA	90	0.0	37.500004	6
CGTTTCA	30	1.1376027E-4	37.500004	1
TACACGG	30	1.1376027E-4	37.500004	2
CTCGTAA	55	2.732122E-9	36.81818	33
CGTAACC	55	2.732122E-9	36.81818	35
TAGCTCG	55	2.732122E-9	36.81818	30
GTAAGGC	80	0.0	36.5625	4
>>END_MODULE