##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288526
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869561148735297	31.0	31.0	34.0	30.0	34.0
2	31.96928179782758	33.0	31.0	34.0	30.0	34.0
3	31.82271961625642	33.0	31.0	34.0	30.0	34.0
4	35.60566465413862	37.0	35.0	37.0	33.0	37.0
5	35.70116384658575	37.0	35.0	37.0	35.0	37.0
6	35.65042318543216	37.0	35.0	37.0	33.0	37.0
7	26.791429541878376	35.0	11.0	37.0	0.0	37.0
8	31.014882540914858	35.0	19.0	37.0	17.0	37.0
9	35.772020545808694	38.0	32.0	39.0	32.0	39.0
10	36.57050664411526	37.0	35.0	39.0	32.0	39.0
11	36.87262153150843	39.0	37.0	39.0	33.0	39.0
12	36.8484088089115	39.0	35.0	39.0	33.0	39.0
13	36.74247034929261	39.0	35.0	39.0	33.0	39.0
14	37.774935361111304	40.0	37.0	41.0	33.0	41.0
15	37.835709780054486	40.0	36.0	41.0	33.0	41.0
16	37.83451058136875	40.0	36.0	41.0	33.0	41.0
17	37.71561315098119	40.0	36.0	41.0	33.0	41.0
18	37.57475929378982	39.0	36.0	41.0	32.0	41.0
19	37.453120342707415	39.0	36.0	41.0	32.0	41.0
20	37.52662498353701	39.0	36.0	41.0	32.0	41.0
21	37.4793363509701	39.0	35.0	41.0	32.0	41.0
22	37.54439461261723	39.0	35.0	41.0	33.0	41.0
23	37.57084976743864	39.0	35.0	41.0	33.0	41.0
24	37.48372763633087	39.0	35.0	41.0	33.0	41.0
25	37.39614454156644	39.0	35.0	41.0	33.0	41.0
26	37.25117667038673	39.0	35.0	41.0	32.0	41.0
27	37.17476761193099	39.0	35.0	41.0	32.0	41.0
28	37.108555208196144	39.0	35.0	41.0	32.0	41.0
29	37.05494132244581	39.0	35.0	41.0	32.0	41.0
30	36.94513146128945	39.0	35.0	41.0	31.0	41.0
31	36.67954014542884	39.0	35.0	41.0	31.0	41.0
32	36.53789606482605	39.0	35.0	40.0	30.0	41.0
33	36.43560372375453	39.0	35.0	40.0	30.0	41.0
34	36.27077629052494	39.0	35.0	40.0	30.0	41.0
35	36.09847639380853	39.0	35.0	40.0	29.0	41.0
36	35.94541219855403	38.0	35.0	40.0	29.0	41.0
37	35.912437700588505	38.0	35.0	40.0	29.0	41.0
38	35.56082987321766	38.0	34.0	40.0	27.0	41.0
39	35.451120522933806	38.0	34.0	40.0	26.0	41.0
40	35.216580134892524	38.0	34.0	40.0	25.0	41.0
41	35.14029584855437	38.0	34.0	40.0	25.0	41.0
42	35.146912236678844	38.0	34.0	40.0	25.0	41.0
43	35.089322972626384	38.0	34.0	40.0	25.0	41.0
44	34.90967885043289	38.0	34.0	40.0	24.0	41.0
45	34.82606073629413	38.0	34.0	40.0	24.0	41.0
46	34.643668161621484	37.0	33.0	40.0	23.0	41.0
47	34.479582429313126	37.0	33.0	40.0	23.0	41.0
48	34.34058975620915	37.0	33.0	40.0	23.0	41.0
49	34.16890332240422	37.0	33.0	40.0	23.0	41.0
50	33.93893098022362	37.0	33.0	40.0	22.0	41.0
51	31.519412461961835	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	12.0
10	14.0
11	14.0
12	9.0
13	7.0
14	19.0
15	20.0
16	36.0
17	81.0
18	141.0
19	286.0
20	409.0
21	677.0
22	1016.0
23	1567.0
24	2188.0
25	2888.0
26	3695.0
27	4663.0
28	5057.0
29	5997.0
30	7242.0
31	8995.0
32	11487.0
33	15344.0
34	22444.0
35	25392.0
36	27451.0
37	40688.0
38	50344.0
39	50326.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60750850876524	17.928020351718736	22.00113681262694	16.463334326889083
2	28.5925705135759	28.206123538259988	25.85763501382891	17.343670934335208
3	30.829457310606323	27.53062115719207	26.334541774398147	15.305379757803456
4	27.33445166120211	23.725418159888537	31.214171339844587	17.725958839064766
5	27.34831522982331	28.01965854030486	24.85564559173177	19.776380638140065
6	25.87739059911412	34.19518518261786	25.76024344426499	14.167180774003036
7	59.18669374683737	27.462689670948198	9.272647872288806	4.077968709925622
8	78.74541635762462	6.916187795900543	9.79911689067883	4.539278955796011
9	73.27277264440639	6.523502214705087	11.026042713654922	9.177682427233593
10	44.06396650561821	22.65965632213388	18.25104150059267	15.02533567165524
11	32.391881494215426	24.05779721758178	24.50177800267567	19.048543285527128
12	29.18281194762344	21.121146794396346	29.662144832701387	20.03389642527883
13	24.972446157365365	22.497799158481385	32.78699319991959	19.742761484233657
14	20.703160200467202	25.72315839820328	31.708407561190327	21.865273840139192
15	19.815198630279422	24.05710403915072	36.3062600944109	19.82143723615896
16	24.997053991667993	25.054587801445972	28.4515087028552	21.49684950403083
17	24.710771299640243	24.437312408587093	28.00371543639048	22.848200855382185
18	25.25491636802229	23.992291855846616	29.401856331838378	21.350935444292716
19	24.448403263484053	26.50471707922336	26.411484580245805	22.635395077046784
20	25.232388069012845	27.501161073872026	28.062982192246107	19.203468664869025
21	24.564164061471065	27.380548026867597	29.158897291751867	18.89639061990947
22	23.58019727858148	22.715803775049736	29.86559270221748	23.838406244151304
23	22.742144555430013	25.840998731483474	29.899905034554948	21.51695167853157
24	24.907980563276794	23.35248816397829	28.70105293803678	23.038478334708138
25	23.289062337536308	27.34173003472824	26.247894470515654	23.1213131572198
26	21.792836694093427	24.652890900646735	27.41797966214483	26.136292743115003
27	22.88944497203025	24.2511939998475	28.078232117729424	24.781128910392823
28	19.889715311618364	24.976951817167254	29.39076547694142	25.742567394272957
29	23.60827100503941	24.07478008914275	26.568142905665347	25.748806000152495
30	23.164636809161046	24.26020531945128	28.93673360459716	23.638424266790516
31	24.531584675211246	22.86449054851209	29.14919279371703	23.454731982559633
32	27.539285887580323	23.936837581361818	26.126241655864636	22.397634875193223
33	24.480982649743872	22.707485633877017	29.562327138628753	23.24920457775036
34	22.31202733895732	22.729667343670933	32.28027976681477	22.678025550556967
35	21.071931125791092	24.511482500710507	28.511122047926357	25.905464325572048
36	22.351191920312207	24.81752077802347	30.505396394085803	22.32589090757852
37	22.54042963199157	25.29927978761013	29.74116717384222	22.419123406556082
38	22.805223792656466	25.200848450399615	28.765865121340884	23.22806263560303
39	24.419982947810595	23.376749409065386	29.160976827045047	23.042290816078967
40	23.732696533414664	22.630889417244894	30.1075119746574	23.528902074683046
41	20.475104496648484	24.905207849552554	29.458350373969765	25.161337279829198
42	21.386287544276772	22.852013336753014	31.15733070849768	24.604368410472542
43	22.721002613282685	22.64475298586609	29.82261563949176	24.81162876135946
44	22.92964932103173	22.627423525089593	28.762399229185586	25.6805279246931
45	21.912409973451265	22.395901929115574	28.041493660883248	27.650194436549913
46	24.138552504800263	24.246688340045612	27.253003195552566	24.36175595960156
47	20.24878173890741	23.472061443336127	32.3568759834469	23.922280834309557
48	21.31558334430866	23.80582685789149	29.191823267227218	25.686766530572637
49	21.86839314307896	21.85834205582859	31.764555014106183	24.50870978698627
50	20.923590941544262	22.985450184732052	30.52376562250889	25.567193251214793
51	20.442525110388665	22.648912056452453	28.93049499871762	27.978067834441262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	259.5
2	444.0
3	1483.5
4	2523.0
5	1702.0
6	881.0
7	773.0
8	665.0
9	643.5
10	622.0
11	597.5
12	573.0
13	598.0
14	623.0
15	623.0
16	623.0
17	637.0
18	651.0
19	678.5
20	706.0
21	751.5
22	797.0
23	940.5
24	1084.0
25	1305.0
26	1858.5
27	2191.0
28	2656.5
29	3122.0
30	3701.5
31	4281.0
32	4953.5
33	5626.0
34	6556.0
35	7486.0
36	7995.0
37	8504.0
38	9092.5
39	9681.0
40	10825.0
41	11969.0
42	13133.0
43	14297.0
44	16326.0
45	18355.0
46	24812.5
47	31270.0
48	27390.5
49	23511.0
50	23481.0
51	23451.0
52	21617.0
53	19783.0
54	18471.5
55	17160.0
56	16527.5
57	15895.0
58	15124.0
59	14353.0
60	13345.5
61	12338.0
62	11316.5
63	10295.0
64	9144.5
65	7994.0
66	6635.5
67	5277.0
68	4442.0
69	3607.0
70	3049.5
71	2492.0
72	2065.0
73	1638.0
74	1327.0
75	781.0
76	546.0
77	409.0
78	272.0
79	212.5
80	153.0
81	122.0
82	91.0
83	63.5
84	36.0
85	28.5
86	21.0
87	14.5
88	8.0
89	8.5
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	288526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.26413470593321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84909176879331	33.71205974216131
2	10.992473849122424	10.610844772109857
3	5.82558920368164	8.435010662037627
4	3.8233250814167548	7.381179070162854
5	2.7261918705170096	6.578864583642649
6	1.8743666279845823	5.427881005281221
7	1.416393060659258	4.7852689833345226
8	1.0256986515658697	3.9603566309495344
9	0.7419921167854282	3.2230446727745194
>10	1.6947433776542242	10.245840616613897
>50	0.010044797273174534	0.34698075398832773
>100	0.0129147393512244	1.2372876586829291
>500	0.0043049131170748	1.2120086217157158
>1k	0.002869942078049867	2.843372226545069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	3192	1.1063127759716629	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	2161	0.7489792947602643	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	1561	0.5410257654422825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1297	0.4495262125423705	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	698	0.24191927243991876	No Hit
GAATCTATCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	628	0.2176580273528209	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	569	0.1972092636365527	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	560	0.19408996069678297	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	544	0.18854453324830345	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	501	0.17364119698051475	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC	425	0.14730041660023707	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTT	416	0.14418111366046735	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	386	0.13378343719456826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.465892155299696E-4	0.0	0.0	0.102590407796871	0.0
2	3.465892155299696E-4	0.0	0.0	0.638070745790674	0.0
3	3.465892155299696E-4	0.0	0.0	0.8124051212022487	0.0
4	3.465892155299696E-4	0.0	0.0	1.2976300229442062	0.0
5	3.465892155299696E-4	0.0	0.0	3.18619465836701	0.0
6	3.465892155299696E-4	0.0	0.0	3.931708060971975	0.0
7	3.465892155299696E-4	0.0	0.0	4.616914940074724	0.0
8	3.465892155299696E-4	0.0	0.0	5.643512196474495	0.0
9	3.465892155299696E-4	0.0	0.0	6.088186159999445	0.0
10	3.465892155299696E-4	0.0	0.0	7.85544456998676	0.0
11	3.465892155299696E-4	0.0	0.0	8.662650852956059	0.0
12	3.465892155299696E-4	0.0	0.0	10.109660827793682	0.0
13	3.465892155299696E-4	0.0	0.0	10.430949030589964	0.0
14	3.465892155299696E-4	0.0	0.0	10.644101398140895	0.0
15	3.465892155299696E-4	0.0	0.0	10.92206594899593	0.0
16	3.465892155299696E-4	0.0	0.0	11.2651892723706	0.0
17	3.465892155299696E-4	0.0	0.0	11.658221442781587	0.0
18	3.465892155299696E-4	0.0	0.0	12.094230675918288	0.0
19	3.465892155299696E-4	0.0	0.0	12.387098563041112	0.0
20	3.465892155299696E-4	0.0	0.0	12.686898234474537	0.0
21	3.465892155299696E-4	0.0	0.0	13.049430553918885	0.0
22	3.465892155299696E-4	0.0	0.0	13.43865024295904	0.0
23	3.465892155299696E-4	0.0	0.0	13.817125666317766	0.0
24	3.465892155299696E-4	0.0	0.0	14.138067279898518	0.0
25	3.465892155299696E-4	0.0	0.0	14.403901208210005	0.0
26	3.465892155299696E-4	0.0	0.0	14.68706459729799	0.0
27	3.465892155299696E-4	0.0	0.0	14.997261945197312	0.0
28	3.465892155299696E-4	0.0	0.0	15.285970761733777	0.0
29	3.465892155299696E-4	0.0	0.0	15.5996340017884	0.0
30	3.465892155299696E-4	0.0	0.0	16.032177342769803	0.0
31	3.465892155299696E-4	0.0	0.0	16.39609601907627	0.0
32	3.465892155299696E-4	0.0	0.0	16.767293068908867	0.0
33	3.465892155299696E-4	0.0	0.0	17.07506429229948	0.0
34	3.465892155299696E-4	0.0	0.0	17.41888079410521	0.0
35	3.465892155299696E-4	0.0	0.0	17.75680527924693	0.0
36	3.465892155299696E-4	0.0	0.0	18.071854876163673	0.0
37	3.465892155299696E-4	0.0	0.0	18.419483859340232	0.0
38	3.465892155299696E-4	0.0	0.0	18.828112544450068	0.0
39	3.465892155299696E-4	0.0	0.0	19.480393448077468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGCG	20	7.024496E-4	45.0	1
AATCTAT	140	0.0	41.785713	2
CTATCTC	175	0.0	41.14286	5
CGCACGG	50	1.0750227E-9	40.5	2
CGTTTTT	535	0.0	40.373833	1
CGAAGGA	45	1.9197614E-8	40.000004	4
TATCTCT	185	0.0	38.918922	6
TACGGCT	250	0.0	38.699997	7
GGACCGA	105	0.0	38.57143	8
TAGACGG	35	6.232034E-6	38.571426	2
GCTACGA	35	6.232034E-6	38.571426	10
ATGCAGT	35	6.232034E-6	38.571426	3
ATCTATC	160	0.0	37.96875	3
GAATCTG	490	0.0	37.65306	1
TACGGGA	60	1.546141E-10	37.499996	4
CATAGGT	30	1.1378038E-4	37.499996	4
CGGATCA	30	1.1378038E-4	37.499996	39
TACCGGG	55	2.733941E-9	36.81818	3
ATAAGGT	25	0.002104499	36.0	4
GCGATGT	25	0.002104499	36.0	9
>>END_MODULE