FastQCFastQC Report
Sat 14 Jan 2017
SRR2936541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272752
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32751.2007244676482665No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.22071332199213936No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.11732269607555582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT13550.042.6752741
CGTTTTA2200.041.931821
TTCGCAA356.230781E-638.57142614
ACAACGA301.13764065E-437.49999613
TTTACGG301.13764065E-437.4999962
CGGCATT301.13764065E-437.49999625
TAGGATC301.13764065E-437.4999966
GCGGGAT1150.037.1739125
CTACGAA552.732122E-936.8181811
AGCTACG552.732122E-936.818189
CGGGTAT250.002104297736.0000046
GACACGA250.002104297736.00000425
GGGTACG250.002104297736.0000047
CACGCCT250.002104297736.00000428
CGACCAA250.002104297736.00000429
TATGCGG250.002104297736.0000042
GCGATTA250.002104297736.0000049
TTAACGG250.002104297736.0000042
CGTGTAG250.002104297736.00000443
TTAGTAG250.002104297736.0000041
Produced by FastQC (version 0.11.5)