##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936541.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272752
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995413415850297	33.0	31.0	34.0	30.0	34.0
2	32.115991816741946	33.0	31.0	34.0	30.0	34.0
3	31.698488003754328	33.0	31.0	34.0	30.0	34.0
4	35.59761248313486	37.0	35.0	37.0	33.0	37.0
5	35.78622704874758	37.0	35.0	37.0	35.0	37.0
6	35.79331773919165	37.0	35.0	37.0	35.0	37.0
7	26.868484924033556	35.0	10.0	37.0	0.0	37.0
8	31.205069073737313	36.0	19.0	37.0	17.0	37.0
9	36.03771924678829	39.0	32.0	39.0	32.0	39.0
10	36.77420880506834	38.0	35.0	39.0	33.0	39.0
11	37.10531911773332	39.0	37.0	39.0	34.0	39.0
12	37.13991464773861	39.0	37.0	39.0	34.0	39.0
13	37.11927685223206	39.0	37.0	39.0	34.0	39.0
14	38.294916260925675	40.0	38.0	41.0	34.0	41.0
15	38.390831231301696	40.0	38.0	41.0	34.0	41.0
16	38.345379685575175	40.0	38.0	41.0	34.0	41.0
17	38.27031515809233	40.0	37.0	41.0	34.0	41.0
18	38.098767378424355	40.0	37.0	41.0	33.0	41.0
19	37.916158268317005	40.0	37.0	41.0	33.0	41.0
20	37.86536853991905	40.0	36.0	41.0	33.0	41.0
21	37.79120593066229	40.0	36.0	41.0	33.0	41.0
22	37.792287499266735	40.0	36.0	41.0	33.0	41.0
23	37.720548336950785	39.0	35.0	41.0	33.0	41.0
24	37.635089751862495	39.0	35.0	41.0	33.0	41.0
25	37.5017085117616	39.0	35.0	41.0	33.0	41.0
26	37.3809027981463	39.0	35.0	41.0	33.0	41.0
27	37.252397782601044	39.0	35.0	41.0	33.0	41.0
28	37.19932392796387	39.0	35.0	41.0	33.0	41.0
29	37.15293746700299	39.0	35.0	41.0	32.0	41.0
30	37.04411333372441	39.0	35.0	41.0	32.0	41.0
31	36.87388176805303	39.0	35.0	41.0	31.0	41.0
32	36.64536282043761	39.0	35.0	41.0	31.0	41.0
33	36.43319572358773	39.0	35.0	41.0	30.0	41.0
34	36.12754443597114	39.0	35.0	41.0	29.0	41.0
35	35.90570188302927	39.0	35.0	41.0	28.0	41.0
36	35.65455065407403	39.0	35.0	41.0	26.0	41.0
37	35.50097891124538	39.0	35.0	40.0	25.0	41.0
38	35.34737783774271	38.0	35.0	40.0	25.0	41.0
39	35.239525283041004	38.0	35.0	40.0	24.0	41.0
40	35.041114272305975	38.0	34.0	40.0	23.0	41.0
41	34.91884569132399	38.0	34.0	40.0	23.0	41.0
42	34.82189314835455	38.0	34.0	40.0	23.0	41.0
43	34.80693817093917	38.0	34.0	40.0	23.0	41.0
44	34.624387722179854	38.0	34.0	40.0	23.0	41.0
45	34.53139482020297	38.0	34.0	40.0	23.0	41.0
46	34.44228089986508	38.0	34.0	40.0	23.0	41.0
47	34.31311961048865	37.0	33.0	40.0	22.0	41.0
48	34.220339355898396	37.0	33.0	40.0	22.0	41.0
49	34.10212574059952	37.0	33.0	40.0	21.0	41.0
50	33.88437848301754	37.0	33.0	40.0	20.0	41.0
51	31.922387370211766	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	19.0
10	28.0
11	25.0
12	13.0
13	11.0
14	15.0
15	35.0
16	46.0
17	74.0
18	139.0
19	227.0
20	459.0
21	667.0
22	1031.0
23	1564.0
24	2334.0
25	3570.0
26	4593.0
27	5011.0
28	5129.0
29	5310.0
30	5945.0
31	6870.0
32	8796.0
33	12257.0
34	18684.0
35	22642.0
36	25531.0
37	39657.0
38	49499.0
39	52550.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01547193054497	18.36686807062826	22.1805889599343	19.437071038892473
2	33.70021118085293	24.075350501554528	25.569015075966444	16.655423241626092
3	31.014621340998417	22.87939226843433	30.506100780195926	15.599885610371325
4	27.023816507303337	24.91054144424239	29.92755323517334	18.138088813280927
5	23.987358479497857	28.985305332316535	28.742960638235466	18.284375549950138
6	25.887986155921862	33.13412917228838	27.563134275825657	13.414750395964099
7	58.813867542676135	27.732152284859506	9.783979585850883	3.6700005866134804
8	80.85733560157212	4.740936821728163	11.274344459435678	3.127383117264035
9	74.92740658180324	6.8839091922332365	13.258564556813516	4.930119669149997
10	42.11004868891887	24.383322578752857	19.929093095559335	13.577535636768934
11	30.811139789992374	24.03428755792808	27.210066287323283	17.944506364756265
12	27.608596820554936	21.98150701003109	31.339091922332372	19.0708042470816
13	24.493679239748932	22.341541033612952	33.16199331260633	20.002786414031796
14	21.250439960110285	24.212104769167595	33.95648794509298	20.580967325629143
15	20.512773508535226	24.070584267026458	35.55794274652431	19.858699477914
16	24.17470815979351	23.658488297061066	32.456590602452046	19.710212940693378
17	25.48285622103596	23.790109696720833	30.035710095617997	20.691323986625214
18	24.95307092156978	23.964993840558456	30.883733208189124	20.19820202968264
19	25.166818208482432	25.093858156860442	29.423798908898924	20.3155247257582
20	26.240320877573765	24.92813984865372	29.93561917052854	18.895920103243974
21	25.85352261394967	24.794685281867775	30.636622279579985	18.71516982460257
22	25.71786824661231	22.4181674194873	31.08941455974658	20.77454977415381
23	24.446750161318707	23.64675602745351	31.36805596292603	20.538437848301754
24	23.345749985334663	23.853903912711914	31.806549539508417	20.993796562445006
25	23.7948759312489	24.778920044582627	29.986214583211122	21.43998944095735
26	22.749970669325982	25.715668446060892	29.564952777614828	21.969408106998298
27	22.674810817152576	24.76352144072271	31.267231770986097	21.29443597113862
28	21.097920455212062	25.755998122836864	31.409118906552475	21.736962515398602
29	22.476462134099844	25.147020003519682	30.573194697014138	21.80332316536634
30	23.049510177743883	23.995424414853055	30.850736199917872	22.104329207485186
31	23.89496685633836	24.114580278054788	30.44963923270957	21.540813632897287
32	24.574338593300872	24.515677245263095	28.843051563324924	22.066932598111105
33	24.035020824778552	25.009899102481377	29.309409280225257	21.645670792514814
34	22.554188420249897	25.558016073209362	30.227459377016487	21.660336129524257
35	22.958951721710566	26.639584677655893	29.265413269196927	21.136050331436618
36	23.73328151580923	26.99815216753681	27.94406640464598	21.324499912007976
37	23.407711034199565	27.782014430691614	27.673857570246962	21.136416964861855
38	23.273523200563147	27.037748577462313	27.416847539156453	22.27188068281809
39	23.386079662110635	26.58715609784713	27.732518918284743	22.294245321757494
40	24.734557400129056	25.478823253358364	28.176511996245672	21.610107350266908
41	21.953642869713146	26.15012905496568	28.724628966973658	23.17159910834751
42	22.320642928374493	25.912917228837916	28.909045579867428	22.85739426292016
43	22.614682935413853	25.730700416495573	28.87458203789523	22.780034610195344
44	22.756203437554994	25.720068047163725	28.4925500087992	23.031178506482078
45	22.203686865724173	25.608978119317182	28.249838681292893	23.93749633366575
46	22.157491054144423	25.87992022056667	28.419589957177216	23.54299876811169
47	21.908913591834338	25.971945210300934	30.051108699477915	22.068032498386813
48	22.315143426995952	25.592112981756323	29.3677039948378	22.72503959640993
49	22.789200445826243	25.01503197043468	29.716372382237346	22.47939520150173
50	21.589942511878924	26.22345574001291	29.310875813926202	22.87572593418197
51	21.603141315187425	25.380565495395086	28.619405173930897	24.396888015486596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	269.5
2	463.0
3	3398.0
4	6333.0
5	4306.0
6	2279.0
7	2016.5
8	1754.0
9	1677.5
10	1601.0
11	1526.5
12	1452.0
13	1389.5
14	1327.0
15	1303.5
16	1280.0
17	1235.5
18	1191.0
19	1208.0
20	1225.0
21	1241.0
22	1257.0
23	1389.0
24	1521.0
25	1686.0
26	2213.0
27	2575.0
28	2988.5
29	3402.0
30	4081.5
31	4761.0
32	5277.5
33	5794.0
34	6468.0
35	7142.0
36	7766.5
37	8391.0
38	9122.0
39	9853.0
40	10795.5
41	11738.0
42	12599.5
43	13461.0
44	14310.5
45	15160.0
46	16492.0
47	17824.0
48	18136.0
49	18448.0
50	18402.5
51	18357.0
52	17599.5
53	16842.0
54	16412.0
55	15982.0
56	15691.0
57	15400.0
58	14854.0
59	14308.0
60	13607.5
61	12907.0
62	11872.5
63	10838.0
64	9474.5
65	8111.0
66	7109.0
67	6107.0
68	5180.5
69	4254.0
70	3556.5
71	2859.0
72	2366.0
73	1873.0
74	1547.0
75	968.0
76	715.0
77	554.0
78	393.0
79	285.5
80	178.0
81	144.5
82	111.0
83	86.5
84	62.0
85	49.0
86	36.0
87	29.0
88	22.0
89	15.5
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	272752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03869376246456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.63634800559561	34.34473198825943
2	11.628138375394204	11.637137103878255
3	6.455148827233352	9.690216460711857
4	3.959597601368419	7.925323671898542
5	2.6908572534779247	6.732349103264418
6	1.9473787902158843	5.846657455387882
7	1.3953885428336132	4.887639398215199
8	1.0597428654501326	4.242251896897269
9	0.6961050656306926	3.1348969385035073
>10	1.5151760477700047	9.387696726130446
>50	0.010989971611465569	0.38751298792532674
>100	0.0036633238704885224	0.36221649202480866
>500	7.326647740977045E-4	0.22070276133495428
>1k	7.326647740977045E-4	1.2006670155680652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	1.2007244676482665	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.22071332199213936	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	320	0.11732269607555582	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02859740716841673	0.0
2	0.0	0.0	0.0	0.12245556402886139	0.0
3	0.0	0.0	0.0	0.1968821493517921	0.0
4	0.0	0.0	0.0	0.3035724760955007	0.0
5	0.0	0.0	0.0	0.6122778201443069	0.0
6	0.0	0.0	0.0	0.9576465067167244	0.0
7	0.0	0.0	0.0	1.2051240687511	0.0
8	0.0	0.0	0.0	1.6890801900627677	0.0
9	0.0	0.0	0.0	1.99815216753681	0.0
10	0.0	0.0	0.0	2.4773420543204083	0.0
11	0.0	0.0	0.0	2.867073385346395	0.0
12	0.0	0.0	0.0	3.217208306446882	0.0
13	0.0	0.0	0.0	3.393925617410688	0.0
14	0.0	0.0	0.0	3.5031823781310494	0.0
15	0.0	0.0	0.0	3.612439138851411	0.0
16	0.0	0.0	0.0	3.7818237813104947	0.0
17	0.0	0.0	0.0	3.976139496685634	0.0
18	0.0	0.0	0.0	4.188786883322579	0.0
19	0.0	0.0	0.0	4.344606089047926	0.0
20	0.0	0.0	0.0	4.513624098081774	0.0
21	0.0	0.0	0.0	4.71197278113451	0.0
22	0.0	0.0	0.0	4.91472106529008	0.0
23	0.0	0.0	0.0	5.14203378893647	0.0
24	0.0	0.0	0.0	5.320584267026456	0.0
25	0.0	0.0	0.0	5.479703173578929	0.0
26	0.0	0.0	0.0	5.657887018243679	0.0
27	0.0	0.0	0.0	5.846336598815041	0.0
28	0.0	0.0	0.0	6.028920044582624	0.0
29	0.0	0.0	0.0	6.228735261336306	0.0
30	0.0	0.0	0.0	6.492711327506306	0.0
31	0.0	0.0	0.0	6.726990086232181	0.0
32	0.0	0.0	0.0	6.955769343579515	0.0
33	0.0	0.0	0.0	7.156317827183669	0.0
34	0.0	0.0	0.0	7.357232944213058	0.0
35	0.0	0.0	0.0	7.589311902387517	0.0
36	0.0	0.0	0.0	7.808192057253476	0.0
37	0.0	0.0	0.0	8.04833695078313	0.0
38	0.0	0.0	0.0	8.327711620813046	0.0
39	0.0	0.0	0.0	8.787103302633895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	1355	0.0	42.675274	1
CGTTTTA	220	0.0	41.93182	1
TTCGCAA	35	6.230781E-6	38.571426	14
ACAACGA	30	1.13764065E-4	37.499996	13
TTTACGG	30	1.13764065E-4	37.499996	2
CGGCATT	30	1.13764065E-4	37.499996	25
TAGGATC	30	1.13764065E-4	37.499996	6
GCGGGAT	115	0.0	37.173912	5
CTACGAA	55	2.732122E-9	36.81818	11
AGCTACG	55	2.732122E-9	36.81818	9
CGGGTAT	25	0.0021042977	36.000004	6
GACACGA	25	0.0021042977	36.000004	25
GGGTACG	25	0.0021042977	36.000004	7
CACGCCT	25	0.0021042977	36.000004	28
CGACCAA	25	0.0021042977	36.000004	29
TATGCGG	25	0.0021042977	36.000004	2
GCGATTA	25	0.0021042977	36.000004	9
TTAACGG	25	0.0021042977	36.000004	2
CGTGTAG	25	0.0021042977	36.000004	43
TTAGTAG	25	0.0021042977	36.000004	1
>>END_MODULE