##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936540.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73941
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07986097023302	33.0	31.0	34.0	30.0	34.0
2	32.1930728553847	33.0	31.0	34.0	30.0	34.0
3	32.09657700058155	33.0	31.0	34.0	30.0	34.0
4	35.8442542026751	37.0	35.0	37.0	35.0	37.0
5	35.90198942400021	37.0	35.0	37.0	35.0	37.0
6	35.872844565261495	37.0	35.0	37.0	35.0	37.0
7	26.762080577757942	36.0	10.0	37.0	0.0	37.0
8	31.177181807116483	36.0	19.0	37.0	17.0	37.0
9	36.032336592688765	39.0	32.0	39.0	32.0	39.0
10	36.84487632030944	38.0	37.0	39.0	34.0	39.0
11	37.1458595366576	39.0	37.0	39.0	34.0	39.0
12	37.04492771263575	39.0	35.0	39.0	34.0	39.0
13	36.86015877523972	39.0	35.0	39.0	33.0	39.0
14	37.98408190313899	40.0	37.0	41.0	33.0	41.0
15	38.167944712676324	40.0	37.0	41.0	33.0	41.0
16	38.14729311207584	40.0	36.0	41.0	34.0	41.0
17	38.078846648003136	40.0	36.0	41.0	34.0	41.0
18	37.985217944036464	40.0	36.0	41.0	34.0	41.0
19	37.83148726687494	40.0	36.0	41.0	33.0	41.0
20	37.8736965959346	40.0	36.0	41.0	34.0	41.0
21	37.82844430018528	40.0	36.0	41.0	33.0	41.0
22	37.81893671981715	40.0	35.0	41.0	33.0	41.0
23	37.76818003543366	40.0	35.0	41.0	33.0	41.0
24	37.70245195493705	40.0	35.0	41.0	33.0	41.0
25	37.62072463180103	39.0	35.0	41.0	33.0	41.0
26	37.45632328478111	39.0	35.0	41.0	33.0	41.0
27	37.34986002353227	39.0	35.0	41.0	33.0	41.0
28	37.34754736884813	39.0	35.0	41.0	33.0	41.0
29	37.34394990600614	39.0	35.0	41.0	33.0	41.0
30	37.30485116511814	39.0	35.0	41.0	33.0	41.0
31	37.177087137041696	39.0	35.0	41.0	33.0	41.0
32	37.02836044954761	39.0	35.0	41.0	32.0	41.0
33	36.94026318280791	39.0	35.0	41.0	32.0	41.0
34	36.79297007073207	39.0	35.0	41.0	31.0	41.0
35	36.70875427705874	39.0	35.0	41.0	31.0	41.0
36	36.53675227546287	39.0	35.0	41.0	31.0	41.0
37	36.44785707523566	39.0	35.0	41.0	30.0	41.0
38	36.38272406378058	39.0	35.0	41.0	30.0	41.0
39	36.36576459609689	39.0	35.0	41.0	30.0	41.0
40	36.196589172448306	39.0	35.0	40.0	30.0	41.0
41	36.1378937260789	38.0	35.0	40.0	30.0	41.0
42	36.10918164482493	38.0	35.0	40.0	30.0	41.0
43	36.014186986922006	38.0	35.0	40.0	30.0	41.0
44	35.86057802842807	38.0	35.0	40.0	29.0	41.0
45	35.74183470604942	38.0	35.0	40.0	29.0	41.0
46	35.648178953489946	38.0	35.0	40.0	29.0	41.0
47	35.54377138529368	38.0	35.0	40.0	28.0	41.0
48	35.49206800016229	38.0	35.0	40.0	28.0	41.0
49	35.371241936138276	38.0	34.0	40.0	28.0	41.0
50	35.11373933271121	37.0	34.0	40.0	27.0	41.0
51	32.99981065985042	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	4.0
11	5.0
12	1.0
13	2.0
14	2.0
15	3.0
16	6.0
17	7.0
18	24.0
19	33.0
20	65.0
21	105.0
22	177.0
23	301.0
24	435.0
25	539.0
26	754.0
27	833.0
28	899.0
29	1094.0
30	1324.0
31	1750.0
32	2308.0
33	3524.0
34	5900.0
35	7673.0
36	6617.0
37	10512.0
38	13847.0
39	15185.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23852801557999	18.518819058438485	23.437605658565612	14.805047267415913
2	30.05098659742227	26.941750855411744	26.366968258476355	16.64029428868963
3	31.123463301821726	24.08000973749341	28.161642390554633	16.63488457013024
4	29.209775361436822	26.333157517480153	26.24254473161034	18.214522389472688
5	27.032363641281563	29.54112062319958	24.437051162413276	18.989464573105586
6	27.24604752437754	35.52426934988707	23.272609242504156	13.957073883231224
7	59.604279087380476	28.737777417129873	7.059682720006491	4.598260775483156
8	80.69271446153013	7.0569778607267954	8.31068013686588	3.939627540877186
9	75.1369335010346	9.003124112468049	9.898432534047416	5.961509852449926
10	46.61013510772102	25.932838344085145	15.527244695094739	11.929781853099094
11	38.01950203540661	24.576351415317617	22.51930593310883	14.884840616166944
12	35.425541986178175	22.738399534764202	25.513585155732272	16.322473323325354
13	25.851692565694268	29.593865379153648	26.08160560446843	18.47283645068365
14	17.915635439066282	33.300875021976985	27.393462355120974	21.390027183835763
15	18.56074437727377	27.2392853761783	36.622442217443634	17.577528029104286
16	20.78549113482371	24.069190300374625	35.25243099227763	19.89288757252404
17	20.600208274164537	23.87579286187636	29.888695040640513	25.63530382331859
18	23.295600546381575	26.062671589510554	28.74318713568926	21.898540728418602
19	27.594974371458324	25.908494610567885	25.862512002813055	20.63401901516074
20	29.03666436753628	25.370227613908387	26.583357000852033	19.009751017703305
21	24.84548491364737	27.623375393895135	26.57659485265279	20.954544839804708
22	23.192815893753128	26.580652141572337	25.393218917785802	24.833313046888737
23	22.3069744796527	27.958777944577434	27.006667478124452	22.72758009764542
24	23.631003097063875	24.318037354106654	30.90166484088665	21.14929470794282
25	21.05462463315346	26.672617357081997	29.386943644256906	22.885814365507635
26	19.73871059358137	30.424257178020316	27.72075032796419	22.116281900434128
27	20.915324380249118	31.063956397668413	28.979862322662665	19.040856899419808
28	19.00028401022437	28.344220391934112	33.20620494718762	19.4492906506539
29	20.18230751545151	26.151931945740525	32.55703872006059	21.10872181874738
30	21.41707577663272	28.038571293328463	29.005558485819776	21.53879444421904
31	24.28422661311045	27.32313601384888	27.225761079779826	21.16687629326084
32	27.36235647340447	27.80054367671522	26.450818896146927	18.386280953733383
33	25.287729405877656	28.8540863661568	24.685898216145304	21.172286011820233
34	21.31699598328397	28.956871018785247	28.240083309665813	21.48604968826497
35	23.618831230305243	26.809212750706646	27.501656726308816	22.070299292679298
36	24.63721074911078	27.17031146454606	28.234673591106425	19.957804195236744
37	22.857413343070824	29.85894158856385	29.017730352578408	18.26591471578691
38	21.46305838438755	29.00285362654008	26.75782042439242	22.776267564679948
39	23.176586738074953	27.231170798339217	28.010170270891656	21.582072192694174
40	23.87444043223651	25.70698259423054	28.62552575702249	21.793051216510463
41	19.558837451481587	27.271743687534656	28.514626526554952	24.6547923344288
42	23.378098754412303	25.900380032728798	27.286620413572983	23.43490079928592
43	24.08406702641295	26.556308408055067	27.6139083864162	21.74571617911578
44	22.089233307637173	28.234673591106425	27.18113090166484	22.494962199591566
45	20.55828295532925	30.494583519292405	25.651532978996766	23.295600546381575
46	21.738954030916542	29.574931364195777	26.724009683396222	21.96210492149146
47	22.315089057491782	27.02830635236202	28.958223448425098	21.6983811417211
48	22.86958520982946	24.726471105340746	29.54112062319958	22.86282306163022
49	21.691618993521864	25.46219282941805	29.013673063658864	23.832515113401225
50	20.325665057275398	27.60444137893726	29.058303241773846	23.011590322013497
51	20.63266658552089	28.601182023505228	25.793538091180807	24.97261329979308
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	106.5
2	172.0
3	292.5
4	413.0
5	335.0
6	257.0
7	227.0
8	197.0
9	180.5
10	164.0
11	187.5
12	211.0
13	193.5
14	176.0
15	192.5
16	209.0
17	219.5
18	230.0
19	239.0
20	248.0
21	313.0
22	378.0
23	361.0
24	344.0
25	389.0
26	623.5
27	813.0
28	979.0
29	1145.0
30	1328.5
31	1512.0
32	1690.0
33	1868.0
34	1958.0
35	2048.0
36	2152.0
37	2256.0
38	2598.0
39	2940.0
40	3495.5
41	4051.0
42	4749.5
43	5448.0
44	5769.0
45	6090.0
46	6347.5
47	6605.0
48	6358.5
49	6112.0
50	5706.0
51	5300.0
52	4841.5
53	4383.0
54	4104.5
55	3826.0
56	3532.0
57	3238.0
58	3214.5
59	3191.0
60	3080.5
61	2970.0
62	2719.0
63	2468.0
64	1974.5
65	1481.0
66	1243.0
67	1005.0
68	823.5
69	642.0
70	558.5
71	475.0
72	380.5
73	286.0
74	222.0
75	118.0
76	78.0
77	61.5
78	45.0
79	28.5
80	12.0
81	8.5
82	5.0
83	7.0
84	9.0
85	6.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	73941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.662636426339915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63565891472868	30.207868435644635
2	10.286458333333332	9.188406973127224
3	5.832122093023256	7.814338459041668
4	4.015261627906977	7.173286809753722
5	2.870639534883721	6.410516492879458
6	2.4012839147286824	6.434860226396721
7	1.6321463178294575	5.1027170311464545
8	1.3202519379844961	4.717274583789778
9	0.9629360465116279	3.8706536292449383
>10	3.0099321705426356	17.638387362897447
>50	0.02422480620155039	0.815515072828336
>100	0.009084302325581396	0.6261749232496179
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.23126546841400575	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	154	0.20827416453659	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	138	0.1866352902990222	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	98	0.13253810470510272	RNA PCR Primer, Index 3 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	91	0.12307109722616683	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	83	0.11225166010738293	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	83	0.11225166010738293	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12036623794647083	0.0
2	0.0	0.0	0.0	0.517980552061779	0.0
3	0.0	0.0	0.0	0.6802721088435374	0.0
4	0.0	0.0	0.0	0.8614976805831677	0.0
5	0.0	0.0	0.0	1.3930025290434265	0.0
6	0.0	0.0	0.0	1.7067662054881594	0.0
7	0.0	0.0	0.0	2.0259396004922845	0.0
8	0.0	0.0	0.0	2.6426475162629663	0.0
9	0.0	0.0	0.0	2.884732421795756	0.0
10	0.0	0.0	0.0	3.6596746054286524	0.0
11	0.0	0.0	0.0	4.34670886247143	0.0
12	0.0	0.0	0.0	5.008046956357096	0.0
13	0.0	0.0	0.0	5.251484291529733	0.0
14	0.0	0.0	0.0	5.371850529476204	0.0
15	0.0	0.0	0.0	5.6017635682503615	0.0
16	0.0	0.0	0.0	6.062942075438525	0.0
17	0.0	0.0	0.0	6.518710864067297	0.0
18	0.0	0.0	0.0	7.069149727485428	0.0
19	0.0	0.0	0.0	7.36938910753168	0.0
20	0.0	0.0	0.0	7.703439228574133	0.0
21	0.0	0.0	0.0	8.084824387011265	0.0
22	0.0	0.0	0.0	8.470266834367942	0.0
23	0.0	0.0	0.0	8.824603400008115	0.0
24	0.0	0.0	0.0	9.155948661770871	0.0
25	0.0	0.0	0.0	9.461597760376517	0.0
26	0.0	0.0	0.0	9.755074992223529	0.0
27	0.0	0.0	0.0	10.067486239028414	0.0
28	0.0	0.0	0.0	10.354201322676188	0.0
29	0.0	0.0	0.0	10.635506687764568	0.0
30	0.0	0.0	0.0	10.976318957006262	0.0
31	0.0	0.0	0.0	11.30631178912917	0.0
32	0.0	0.0	0.0	11.593026872776944	0.0
33	0.0	0.0	0.0	11.900028401022437	0.0
34	0.0	0.0	0.0	12.259774685222002	0.0
35	0.0	0.0	0.0	12.593824806264454	0.0
36	0.0	0.0	0.0	12.967095386862498	0.0
37	0.0	0.0	0.0	13.288973641146319	0.0
38	0.0	0.0	0.0	13.57704115443394	0.0
39	0.0	0.0	0.0	13.902976697637305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	20	6.9904746E-4	45.000004	5
CTATGCC	20	6.9904746E-4	45.000004	9
ACTATGC	20	6.9904746E-4	45.000004	8
AGGCGAT	40	6.666596E-9	45.000004	7
ACGGGAC	20	6.9904746E-4	45.000004	5
AATATAC	20	6.9904746E-4	45.000004	16
AACGAGG	20	6.9904746E-4	45.000004	2
AGCGACT	20	6.9904746E-4	45.000004	19
ATTGGGC	20	6.9904746E-4	45.000004	4
CTCGTGT	25	3.855364E-5	45.0	16
TTACACG	25	3.855364E-5	45.0	34
TACACGC	25	3.855364E-5	45.0	35
CGAGGGA	50	1.0531949E-9	40.5	4
GGGGTAA	40	3.4000368E-7	39.375004	7
GTACACT	30	1.1296185E-4	37.499996	18
CTGAGGG	30	1.1296185E-4	37.499996	3
TCTTACA	30	1.1296185E-4	37.499996	32
CCTGTAC	30	1.1296185E-4	37.499996	15
TGTCTTA	30	1.1296185E-4	37.499996	30
TGGGCGA	30	1.1296185E-4	37.499996	6
>>END_MODULE