FastQCFastQC Report
Sat 14 Jan 2017
SRR2936539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51871
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.30460180062077075No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC1520.29303464363517184No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG1450.27953962715197317No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1310.25254959418557577No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC930.1792909332767828No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC670.1291665863391876No Hit
GAACTTGGGAATTTACCAGAATACAGACACAAAAAGACCAAAATGAATATT600.11567156985598889No Hit
GGGCAAGGGAACACAAAACAAACATCAACAGATACAAGAACATTGAATTAA540.10410441287039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCTT206.971076E-445.00000431
GAAAATC206.971076E-445.00000429
GGATTGT206.971076E-445.0000048
AGGCGAT206.971076E-445.0000047
CAAAGCC206.971076E-445.00000417
GGGACCC206.971076E-445.0000048
AGTTGAT206.971076E-445.00000443
AAAATCT206.971076E-445.00000430
GGGAATA406.6011125E-945.0000047
CTTTGAG206.971076E-445.00000435
ACGGCTG206.971076E-445.0000048
GGAACCT206.971076E-445.0000049
TTCGGGG206.971076E-445.0000044
CGCAAGG206.971076E-445.0000043
AAGTTGA206.971076E-445.00000442
AACACAA206.971076E-445.00000410
ATGGGCT351.1821976E-745.05
GGGCACA253.8393355E-545.07
GCGATGC253.8393355E-545.09
GGAATAG253.8393355E-545.08