##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936539.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51871
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.042663530681885	33.0	31.0	34.0	30.0	34.0
2	32.16864914885003	33.0	31.0	34.0	30.0	34.0
3	32.04865917371942	33.0	31.0	34.0	30.0	34.0
4	35.793256347477396	37.0	35.0	37.0	35.0	37.0
5	35.87422644637659	37.0	35.0	37.0	35.0	37.0
6	35.844922982013074	37.0	35.0	37.0	35.0	37.0
7	26.926028031077095	36.0	11.0	37.0	0.0	37.0
8	31.26538913843959	36.0	19.0	37.0	17.0	37.0
9	36.092652927454644	39.0	32.0	39.0	32.0	39.0
10	36.77658036282316	38.0	35.0	39.0	33.0	39.0
11	37.07776985213318	39.0	37.0	39.0	34.0	39.0
12	36.848759422413295	39.0	35.0	39.0	33.0	39.0
13	36.52991074010526	39.0	35.0	39.0	33.0	39.0
14	37.640531318077535	40.0	36.0	41.0	33.0	41.0
15	37.84347708738987	40.0	36.0	41.0	33.0	41.0
16	37.90563127759249	40.0	35.0	41.0	33.0	41.0
17	37.836131942704014	40.0	35.0	41.0	33.0	41.0
18	37.78544851651212	39.0	36.0	41.0	33.0	41.0
19	37.65689884521216	39.0	36.0	41.0	33.0	41.0
20	37.702222822000735	39.0	35.0	41.0	34.0	41.0
21	37.61760906865108	39.0	35.0	41.0	33.0	41.0
22	37.60455745985232	39.0	35.0	41.0	33.0	41.0
23	37.578492799444774	39.0	35.0	41.0	33.0	41.0
24	37.50575466060034	39.0	35.0	41.0	33.0	41.0
25	37.42902585259586	39.0	35.0	41.0	33.0	41.0
26	37.28746312968711	39.0	35.0	41.0	33.0	41.0
27	37.16448497233522	39.0	35.0	41.0	33.0	41.0
28	37.16028223863045	39.0	35.0	41.0	33.0	41.0
29	37.244124848181066	39.0	35.0	41.0	33.0	41.0
30	37.184573268300205	39.0	35.0	41.0	33.0	41.0
31	37.005783578492796	39.0	35.0	41.0	32.0	41.0
32	36.83393418287675	39.0	35.0	41.0	32.0	41.0
33	36.700873320352414	39.0	35.0	41.0	31.0	41.0
34	36.57945672919357	39.0	35.0	41.0	31.0	41.0
35	36.515721694202924	39.0	35.0	41.0	31.0	41.0
36	36.326020319639106	38.0	35.0	40.0	30.0	41.0
37	36.24823118891095	38.0	35.0	40.0	30.0	41.0
38	36.11416783944786	38.0	35.0	40.0	30.0	41.0
39	36.12436621619016	38.0	35.0	40.0	30.0	41.0
40	35.98376742303021	38.0	35.0	40.0	30.0	41.0
41	35.93763374525265	38.0	35.0	40.0	30.0	41.0
42	35.88317171444545	38.0	35.0	40.0	30.0	41.0
43	35.788745156253015	38.0	35.0	40.0	29.0	41.0
44	35.56858360162711	38.0	35.0	40.0	29.0	41.0
45	35.49615392030229	38.0	34.0	40.0	28.0	41.0
46	35.41433556322415	38.0	34.0	40.0	28.0	41.0
47	35.27747681748954	38.0	34.0	40.0	27.0	41.0
48	35.21844575967304	37.0	34.0	40.0	27.0	41.0
49	35.130766709722195	37.0	34.0	40.0	27.0	41.0
50	34.87413005340171	37.0	34.0	40.0	26.0	41.0
51	32.72402691291859	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	5.0
12	0.0
13	2.0
14	2.0
15	2.0
16	5.0
17	10.0
18	17.0
19	25.0
20	46.0
21	92.0
22	139.0
23	192.0
24	273.0
25	390.0
26	505.0
27	648.0
28	740.0
29	843.0
30	1061.0
31	1333.0
32	1773.0
33	2557.0
34	4504.0
35	6144.0
36	4406.0
37	6930.0
38	9233.0
39	9987.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34817142526653	20.169266063889264	24.406701239613657	15.075861271230554
2	28.243141639837287	27.597308708141348	28.127470069981296	16.03207958204006
3	30.895876308534636	25.33592951745677	27.693701683021345	16.074492490987257
4	28.518825547994066	25.048678452314395	27.67827880704054	18.754217192650998
5	26.88400069402942	29.25912359507239	25.748491449943128	18.108384260955063
6	26.0453818125735	35.26633378959341	24.936862601453605	13.751421796379478
7	60.60033544755258	28.23735806134449	6.77449827456575	4.387808216537178
8	83.3567889572208	5.5194617416282705	7.4974455861656795	3.6263037149852515
9	79.198396020898	6.440978581480981	8.659944863218369	5.700680534402653
10	48.653390140926525	22.259065759287463	15.606022633070502	13.481521466715506
11	40.67783539935609	25.459312525303158	20.539415087428427	13.323436987912322
12	36.573422528966084	23.463977945287347	24.233193884829674	15.729405640916891
13	24.92143972547281	32.2877908658017	25.893080912263116	16.89768849646238
14	17.81534961731989	36.209057083919724	27.373677006419772	18.601916292340615
15	16.90925565344798	27.0459408918278	40.296119218831336	15.748684235892888
16	19.41740085982534	22.127971313450676	38.788533091708274	19.66609473501571
17	18.84675444853579	22.08363054500588	30.516087987507472	28.55352701895086
18	23.379152127392956	24.429835553584855	30.660677449827457	21.530334869194732
19	26.309498563744675	26.27479709278788	26.95533149544061	20.460372848026836
20	28.61329066337645	25.70993425999113	27.040157313335005	18.636617763297412
21	23.86111700179291	27.379460584912575	28.275915251296485	20.483507161998034
22	21.709625802471518	24.720942337722427	28.514969828998865	25.054462030807194
23	21.081143606253978	28.1120471940005	26.939908619459814	23.86690058028571
24	21.80987449634671	24.879026816525613	31.53014208324497	21.78095660388271
25	19.561990322145324	25.42653891384396	32.15476856046731	22.856702203543406
26	19.452102330782132	31.001908580902626	28.52846484548206	21.017524242833183
27	20.51435291395963	31.913786123267336	28.106263615507704	19.46559734726533
28	16.28077345723044	29.409496635885173	34.3775905611999	19.932139345684487
29	19.17641842262536	27.362109849434173	32.16247999845771	21.298991729482754
30	21.792523760868306	28.181450135914094	30.257754814829095	19.768271288388505
31	25.079524204275994	28.923676042490023	25.10458637774479	20.892213375489195
32	26.793391297642227	31.77112452044495	23.733878274951323	17.7016059069615
33	26.39046866264387	29.68325268454435	24.26596749628887	19.660311156522916
34	20.550982244414026	30.670316747315457	26.814597752115827	21.96410325615469
35	20.714850301710012	29.51552890825317	27.491276435773358	22.27834435426346
36	26.0376703745831	28.042644252086905	26.722060496231038	19.197624877098956
37	23.194077615623375	29.216710686125197	28.52460912648686	19.06460257176457
38	21.443581191802743	30.670316747315457	25.156638584179987	22.729463476701817
39	22.409438800100247	28.23157448285169	27.880704054288525	21.478282662759536
40	24.26596749628887	27.221376106109386	27.884559773283723	20.628096624318022
41	19.839602089799698	26.074299705037497	27.400667039386168	26.68543116577664
42	23.134313971197777	26.95147577644541	27.078714503287	22.83549574906981
43	25.10265851824719	26.79917487613503	25.947060978195907	22.151105627421874
44	22.104836999479478	29.731449171984348	27.1095602552486	21.05415357328758
45	20.250236162788458	31.44338840585298	24.321875421719266	23.984500009639298
46	23.167087582656976	30.321374178249886	25.495941855757554	21.015596383335584
47	21.35489965491315	28.84656166258603	28.401226118640473	21.397312563860346
48	23.35987353241696	26.006824622621505	28.326039598234082	22.307262246727458
49	21.919762487709896	23.296254168996164	30.438973607603476	24.34500973569046
50	19.8049006188429	28.526536985984464	28.370380366678877	23.298182028493763
51	19.895510015230087	29.636984056601957	25.474735401283954	24.992770526884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	52.0
2	78.0
3	201.0
4	324.0
5	234.5
6	145.0
7	143.0
8	141.0
9	125.0
10	109.0
11	109.5
12	110.0
13	127.0
14	144.0
15	134.0
16	124.0
17	133.5
18	143.0
19	150.5
20	158.0
21	194.5
22	231.0
23	233.5
24	236.0
25	324.0
26	481.5
27	551.0
28	634.0
29	717.0
30	796.0
31	875.0
32	945.5
33	1016.0
34	1176.5
35	1337.0
36	1503.5
37	1670.0
38	1847.5
39	2025.0
40	2478.5
41	2932.0
42	3443.0
43	3954.0
44	4338.0
45	4722.0
46	5142.0
47	5562.0
48	5175.0
49	4788.0
50	4479.0
51	4170.0
52	3795.5
53	3421.0
54	3022.5
55	2624.0
56	2376.5
57	2129.0
58	2043.5
59	1958.0
60	1887.5
61	1817.0
62	1620.0
63	1423.0
64	1103.0
65	783.0
66	584.5
67	386.0
68	329.0
69	272.0
70	218.5
71	165.0
72	126.5
73	88.0
74	74.5
75	43.0
76	25.0
77	14.5
78	4.0
79	6.0
80	8.0
81	5.5
82	3.0
83	3.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	51871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.68641437412042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6430078487636	31.817393148387346
2	9.713899907165162	8.875865126949547
3	5.105916111064225	6.998129976287328
4	3.5150645624103296	6.423627846002583
5	2.865220693729429	6.545082994351372
6	2.0128280867583763	5.51753388213067
7	1.721664275466284	5.505966725145071
8	1.3207865642670267	4.8273601819899365
9	1.0169634568318002	4.181527250293999
>10	3.0508903704954005	17.649553700526305
>50	0.016879061524179256	0.5282335023423493
>100	0.016879061524179256	1.1297256655934915
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.30460180062077075	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	152	0.29303464363517184	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	145	0.27953962715197317	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	131	0.25254959418557577	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	93	0.1792909332767828	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	67	0.1291665863391876	No Hit
GAACTTGGGAATTTACCAGAATACAGACACAAAAAGACCAAAATGAATATT	60	0.11567156985598889	No Hit
GGGCAAGGGAACACAAAACAAACATCAACAGATACAAGAACATTGAATTAA	54	0.10410441287039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1291665863391876	0.0
2	0.0	0.0	0.0	0.5879971467679436	0.0
3	0.0	0.0	0.0	0.7653602205471265	0.0
4	0.0	0.0	0.0	1.0024869387519038	0.0
5	0.0	0.0	0.0	1.7659192998014304	0.0
6	0.0	0.0	0.0	2.1379961828381946	0.0
7	0.0	0.0	0.0	2.5158566443677586	0.0
8	0.0	0.0	0.0	3.2445875344604884	0.0
9	0.0	0.0	0.0	3.518343583119662	0.0
10	0.0	0.0	0.0	4.58830560428756	0.0
11	0.0	0.0	0.0	5.502111006149872	0.0
12	0.0	0.0	0.0	6.543155134853772	0.0
13	0.0	0.0	0.0	6.919087736885736	0.0
14	0.0	0.0	0.0	7.092595091669719	0.0
15	0.0	0.0	0.0	7.3779182973144914	0.0
16	0.0	0.0	0.0	7.904223940159241	0.0
17	0.0	0.0	0.0	8.482581789439186	0.0
18	0.0	0.0	0.0	9.201673382043916	0.0
19	0.0	0.0	0.0	9.58146170307108	0.0
20	0.0	0.0	0.0	9.926548553141448	0.0
21	0.0	0.0	0.0	10.364172659096605	0.0
22	0.0	0.0	0.0	10.857704690482157	0.0
23	0.0	0.0	0.0	11.35509244086291	0.0
24	0.0	0.0	0.0	11.756087216363671	0.0
25	0.0	0.0	0.0	12.054905438491643	0.0
26	0.0	0.0	0.0	12.346012222629215	0.0
27	0.0	0.0	0.0	12.679531915713984	0.0
28	0.0	0.0	0.0	12.993773013822752	0.0
29	0.0	0.0	0.0	13.348499161381119	0.0
30	0.0	0.0	0.0	13.728287482408282	0.0
31	0.0	0.0	0.0	14.07723005147385	0.0
32	0.0	0.0	0.0	14.445451215515414	0.0
33	0.0	0.0	0.0	14.78282662759538	0.0
34	0.0	0.0	0.0	15.141408494148946	0.0
35	0.0	0.0	0.0	15.540475410152109	0.0
36	0.0	0.0	0.0	15.964604499624068	0.0
37	0.0	0.0	0.0	16.340537101656032	0.0
38	0.0	0.0	0.0	16.716469703687995	0.0
39	0.0	0.0	0.0	17.036494380289565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCTT	20	6.971076E-4	45.000004	31
GAAAATC	20	6.971076E-4	45.000004	29
GGATTGT	20	6.971076E-4	45.000004	8
AGGCGAT	20	6.971076E-4	45.000004	7
CAAAGCC	20	6.971076E-4	45.000004	17
GGGACCC	20	6.971076E-4	45.000004	8
AGTTGAT	20	6.971076E-4	45.000004	43
AAAATCT	20	6.971076E-4	45.000004	30
GGGAATA	40	6.6011125E-9	45.000004	7
CTTTGAG	20	6.971076E-4	45.000004	35
ACGGCTG	20	6.971076E-4	45.000004	8
GGAACCT	20	6.971076E-4	45.000004	9
TTCGGGG	20	6.971076E-4	45.000004	4
CGCAAGG	20	6.971076E-4	45.000004	3
AAGTTGA	20	6.971076E-4	45.000004	42
AACACAA	20	6.971076E-4	45.000004	10
ATGGGCT	35	1.1821976E-7	45.0	5
GGGCACA	25	3.8393355E-5	45.0	7
GCGATGC	25	3.8393355E-5	45.0	9
GGAATAG	25	3.8393355E-5	45.0	8
>>END_MODULE