##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255458
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04654385456709	33.0	31.0	34.0	30.0	34.0
2	32.17099484063917	33.0	31.0	34.0	30.0	34.0
3	32.044899748686674	33.0	31.0	34.0	30.0	34.0
4	35.79460811561979	37.0	35.0	37.0	35.0	37.0
5	35.87039356763147	37.0	35.0	37.0	35.0	37.0
6	35.82597139255768	37.0	35.0	37.0	35.0	37.0
7	26.751736097518965	35.0	10.0	37.0	0.0	37.0
8	31.09788301795207	35.0	19.0	37.0	17.0	37.0
9	35.94851991325384	39.0	32.0	39.0	32.0	39.0
10	36.70883667765347	38.0	35.0	39.0	33.0	39.0
11	37.04042543196925	39.0	37.0	39.0	34.0	39.0
12	37.06783894025632	39.0	37.0	39.0	34.0	39.0
13	37.01570512569581	39.0	37.0	39.0	33.0	39.0
14	38.213549781177335	40.0	37.0	41.0	33.0	41.0
15	38.27025577590054	40.0	37.0	41.0	33.0	41.0
16	38.21203876958248	40.0	37.0	41.0	33.0	41.0
17	38.09948406391657	40.0	37.0	41.0	33.0	41.0
18	37.989285910012605	40.0	37.0	41.0	33.0	41.0
19	37.8578279012597	40.0	37.0	41.0	33.0	41.0
20	37.9559457914804	40.0	36.0	41.0	33.0	41.0
21	37.8652694376375	40.0	36.0	41.0	33.0	41.0
22	37.88477557954732	40.0	36.0	41.0	33.0	41.0
23	37.83837264834141	40.0	36.0	41.0	33.0	41.0
24	37.7593028991067	40.0	36.0	41.0	33.0	41.0
25	37.639850777818666	40.0	36.0	41.0	33.0	41.0
26	37.52381604803921	40.0	36.0	41.0	33.0	41.0
27	37.41053715287836	39.0	35.0	41.0	33.0	41.0
28	37.3869638061834	39.0	36.0	41.0	33.0	41.0
29	37.36273673167409	40.0	35.0	41.0	33.0	41.0
30	37.23351392401099	39.0	35.0	41.0	32.0	41.0
31	37.11068355659247	39.0	35.0	41.0	32.0	41.0
32	36.957965692990626	39.0	35.0	41.0	31.0	41.0
33	36.818572916095796	39.0	35.0	41.0	31.0	41.0
34	36.60855013348574	39.0	35.0	41.0	30.0	41.0
35	36.45648208316044	39.0	35.0	41.0	30.0	41.0
36	36.35141197378825	39.0	35.0	41.0	30.0	41.0
37	36.28012824025867	39.0	35.0	41.0	30.0	41.0
38	36.10448684323842	39.0	35.0	40.0	29.0	41.0
39	36.0545843152299	39.0	35.0	40.0	29.0	41.0
40	35.82280061693116	38.0	35.0	40.0	28.0	41.0
41	35.73800389887966	38.0	35.0	40.0	28.0	41.0
42	35.65215025561932	38.0	35.0	40.0	27.0	41.0
43	35.624768063634725	38.0	35.0	40.0	27.0	41.0
44	35.44130542006905	38.0	34.0	40.0	27.0	41.0
45	35.31559003828418	38.0	34.0	40.0	26.0	41.0
46	35.14621581629857	38.0	34.0	40.0	26.0	41.0
47	34.997651277313686	38.0	34.0	40.0	25.0	41.0
48	34.911793719515536	37.0	34.0	40.0	25.0	41.0
49	34.76057512389512	37.0	34.0	40.0	24.0	41.0
50	34.56048743824817	37.0	34.0	40.0	24.0	41.0
51	32.429362165209156	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	21.0
10	31.0
11	23.0
12	11.0
13	10.0
14	17.0
15	20.0
16	40.0
17	71.0
18	120.0
19	260.0
20	367.0
21	549.0
22	785.0
23	1099.0
24	1563.0
25	2251.0
26	2673.0
27	3242.0
28	3778.0
29	4262.0
30	5230.0
31	6617.0
32	8532.0
33	11953.0
34	18447.0
35	21973.0
36	24674.0
37	38673.0
38	48011.0
39	50134.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.527538773497014	18.56782719664289	23.022962678796517	15.88167135106358
2	29.59116567106922	25.983136171112275	26.53547745617675	17.890220701641756
3	31.277548559841538	24.98414612186739	26.991129657321363	16.74717566096971
4	27.971329925075743	25.354852852523702	27.383366345935535	19.290450876465016
5	26.154984380994133	29.298358242842266	24.954787088288484	19.59187028787511
6	27.370056917379763	34.254163110961485	24.05640066077398	14.319379310884765
7	61.105543768447255	28.11890800053238	6.782328210508186	3.993220020512178
8	82.95062202005809	5.589568539642524	7.699895873294241	3.759913567005144
9	77.14223081680746	6.973357655661597	9.473964409022226	6.410447118508718
10	43.95360489786971	24.6361437105121	16.151383006208455	15.258868385409736
11	32.219777810833875	24.64162406344683	23.784340282942793	19.354257842776505
12	28.46730186566872	22.490976990346752	28.394100008611982	20.647621135372546
13	24.806817559050803	23.727970938471294	30.625386560608792	20.839824941869114
14	21.556185361194405	26.12758261632049	30.43670583814169	21.879526184343415
15	20.62961426144415	24.151523929569635	33.80203399384635	21.416827815139865
16	24.57194529041956	24.884325407699112	29.499956940084083	21.04377236179724
17	25.373251180233147	24.386004744419825	27.595142841484705	22.645601233862315
18	26.476759389018937	23.52089188829475	28.651285142763193	21.351063579923117
19	25.7893665494915	26.223097338897194	26.132671515474172	21.854864596137134
20	28.51114468914655	25.479726608679314	26.418824229423233	19.5903044727509
21	26.670529010639715	26.106444112143677	27.2350053629168	19.988021514299806
22	27.46713745508068	22.488236813879386	27.237354085603112	22.807271645436824
23	25.140336180507166	24.845180029593905	27.800656076537045	22.213827713361884
24	25.028771852907326	23.0484071745649	29.01886024317109	22.903960729356683
25	24.043874139780318	25.870006028388225	27.315253388032474	22.770866443798983
26	23.809784778711176	25.71499033109161	26.707717119839657	23.767507770357554
27	23.353741123785515	25.71538178487266	28.05314376531563	22.877733326026195
28	21.075871571843514	26.935151766630916	28.62466628565165	23.36431037587392
29	23.699394812454493	24.887065584166475	27.81279114374966	23.600748459629372
30	24.135082870765448	24.477996382967063	28.6994339578326	22.687486788434892
31	25.14072763428822	24.578208550916393	27.379451808125015	22.901612006670373
32	25.467200087685647	24.65297622309734	27.83197237902121	22.047851310195803
33	25.87587783510401	24.891763029539103	26.579711733435634	22.652647401921257
34	24.08458533300973	25.062045424296752	28.682601445247357	22.170767797446157
35	23.31107266165084	26.496723531852595	28.06449592496614	22.12770788153043
36	24.943434928637977	26.886220043999405	26.365977968981202	21.804367058381416
37	24.13860595479492	26.89287475827729	27.30468413594407	21.663835150983722
38	24.18323168583485	26.873302069224685	26.521385120058877	22.422081124881586
39	24.730092617964598	25.311792936607976	27.17824456466425	22.77986988076318
40	25.83086065028302	24.587994895442694	27.16532658988953	22.415817864384753
41	22.280374856140735	25.825771751129345	26.834939598681583	25.058913794048337
42	23.027660124169138	25.47385480196353	27.640551480086746	23.857933593780583
43	23.832880551793252	23.82583438373431	27.39158687533763	24.949698189134807
44	23.287585434787715	23.88416099711107	28.116950731627117	24.711302836474097
45	23.63245621589459	24.518707576196476	27.23813699316522	24.610699214743715
46	23.504450829490562	25.91815484345763	26.50181243100627	24.075581896045534
47	22.424429847567897	25.561148995138144	28.619185932716924	23.395235224577036
48	23.13570136773951	24.744967861644575	28.68455871415262	23.434772056463295
49	23.668469963751377	24.308888349552568	28.726835722506245	23.29580596418981
50	21.929240814537028	25.621432877420165	28.58630381510855	23.86302249293426
51	22.569267746557166	24.606393223152143	27.090167463927532	25.734171566363162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	235.0
2	403.0
3	1021.5
4	1640.0
5	1141.5
6	643.0
7	572.5
8	502.0
9	499.5
10	497.0
11	541.5
12	586.0
13	537.0
14	488.0
15	504.5
16	521.0
17	533.0
18	545.0
19	585.0
20	625.0
21	719.5
22	814.0
23	931.5
24	1049.0
25	1297.0
26	1866.0
27	2187.0
28	2598.0
29	3009.0
30	3512.0
31	4015.0
32	4481.0
33	4947.0
34	5941.5
35	6936.0
36	7472.0
37	8008.0
38	8872.0
39	9736.0
40	10516.5
41	11297.0
42	12419.5
43	13542.0
44	14575.0
45	15608.0
46	17594.5
47	19581.0
48	19143.0
49	18705.0
50	18555.0
51	18405.0
52	17724.0
53	17043.0
54	16245.5
55	15448.0
56	14973.0
57	14498.0
58	14147.0
59	13796.0
60	13334.5
61	12873.0
62	11622.5
63	10372.0
64	9412.0
65	8452.0
66	7394.0
67	6336.0
68	5259.5
69	4183.0
70	3397.5
71	2612.0
72	2116.0
73	1620.0
74	1370.0
75	838.5
76	557.0
77	424.5
78	292.0
79	226.0
80	160.0
81	121.0
82	82.0
83	67.0
84	52.0
85	38.0
86	24.0
87	19.5
88	15.0
89	12.5
90	10.0
91	8.0
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	255458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.33892925211225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.71186343825351	26.128142201282284
2	12.796385770523289	10.83570543641843
3	7.351951112878751	9.338212140994843
4	4.880923459540436	8.266122921538301
5	3.45506778527431	7.314193526099058
6	2.4360345428509507	6.188345649928081
7	1.9226271792782195	5.698138328515407
8	1.4580670378997196	4.938639772997848
9	1.1334949940724637	4.319186792549169
>10	2.8257932491706432	14.964587540538993
>50	0.015880817290101424	0.4580566948033404
>100	0.008932959725682052	0.6786803322086189
>500	0.002977653241894017	0.8719886621256249
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.2959390584753658	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG	676	0.2646227559912001	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	643	0.25170478121648177	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC	441	0.1726311174439634	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.053629168004133754	0.0
2	0.0	0.0	0.0	0.27793218454697055	0.0
3	0.0	0.0	0.0	0.3757956298099883	0.0
4	0.0	0.0	0.0	0.5699567052118156	0.0
5	0.0	0.0	0.0	1.2561751833960964	0.0
6	0.0	0.0	0.0	1.6123981241534813	0.0
7	0.0	0.0	0.0	1.9533543674498353	0.0
8	0.0	0.0	0.0	2.5123503667921927	0.0
9	0.0	0.0	0.0	2.773058584972872	0.0
10	0.0	0.0	0.0	3.5829764579696075	0.0
11	0.0	0.0	0.0	4.130228843880403	0.0
12	0.0	0.0	0.0	4.7964831792310285	0.0
13	0.0	0.0	0.0	5.001604960502314	0.0
14	0.0	0.0	0.0	5.104557304919008	0.0
15	0.0	0.0	0.0	5.2944123887292625	0.0
16	0.0	0.0	0.0	5.576259111086754	0.0
17	0.0	0.0	0.0	5.927784606471514	0.0
18	0.0	0.0	0.0	6.306320412748867	0.0
19	0.0	0.0	0.0	6.560373916651661	0.0
20	0.0	0.0	0.0	6.8054239835902575	0.0
21	0.0	0.0	0.0	7.119761369775071	0.0
22	0.0	0.0	0.0	7.45406289879354	0.0
23	0.0	0.0	0.0	7.761354116919415	0.0
24	0.0	0.0	0.0	8.016581982165366	0.0
25	0.0	0.0	0.0	8.235013191992422	0.0
26	0.0	0.0	0.0	8.460099116097362	0.0
27	0.0	0.0	0.0	8.687925216669667	0.0
28	0.0	0.0	0.0	8.928277838235639	0.0
29	0.0	0.0	0.0	9.16080138418057	0.0
30	0.0	0.0	0.0	9.457914803999092	0.0
31	0.0	0.0	0.0	9.70883667765347	0.0
32	0.0	0.0	0.0	9.984420139514128	0.0
33	0.0	0.0	0.0	10.246302719037963	0.0
34	0.0	0.0	0.0	10.522669088460725	0.0
35	0.0	0.0	0.0	10.806864533504529	0.0
36	0.0	0.0	0.0	11.0945830625778	0.0
37	0.0	0.0	0.0	11.399134104236312	0.0
38	0.0	0.0	0.0	11.701727876989564	0.0
39	0.0	0.0	0.0	12.106491086597405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAAGAG	20	7.022975E-4	45.000004	1
TACACGG	20	7.022975E-4	45.000004	2
ACGACAT	20	7.022975E-4	45.000004	42
CGAATAT	25	3.8822625E-5	45.0	14
TCAAGCG	25	3.8822625E-5	45.0	17
AACACGT	25	3.8822625E-5	45.0	41
CGTTTTT	335	0.0	41.641792	1
AGCGACT	55	6.002665E-11	40.909092	19
AGGCGAT	90	0.0	40.0	7
TCATGCA	45	1.9183062E-8	40.0	24
AGTACGG	45	1.9183062E-8	40.0	2
CAAGCTT	40	3.4441655E-7	39.375004	22
GCGATCA	35	6.2292274E-6	38.57143	9
CGCACTT	35	6.2292274E-6	38.57143	35
AAGGATC	35	6.2292274E-6	38.57143	6
CGACCAA	30	1.137438E-4	37.500004	29
TCGATCA	30	1.137438E-4	37.500004	17
CGAGACA	30	1.137438E-4	37.500004	22
CGCACGG	30	1.137438E-4	37.500004	2
CGCACGA	30	1.137438E-4	37.500004	40
>>END_MODULE