##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936532.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324370
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0161728889848	33.0	31.0	34.0	30.0	34.0
2	32.131192773684376	33.0	31.0	34.0	30.0	34.0
3	31.631978912969757	33.0	31.0	34.0	30.0	34.0
4	35.56887196719795	37.0	35.0	37.0	33.0	37.0
5	35.79680919937109	37.0	35.0	37.0	35.0	37.0
6	35.809489163609456	37.0	35.0	37.0	35.0	37.0
7	26.923365292721275	35.0	11.0	37.0	0.0	37.0
8	31.241135123470112	36.0	19.0	37.0	17.0	37.0
9	36.07946172580695	39.0	32.0	39.0	32.0	39.0
10	36.80464901193082	38.0	35.0	39.0	34.0	39.0
11	37.15895119770632	39.0	37.0	39.0	34.0	39.0
12	37.25888029102568	39.0	37.0	39.0	34.0	39.0
13	37.29567469248081	39.0	37.0	39.0	34.0	39.0
14	38.553204673675125	40.0	38.0	41.0	35.0	41.0
15	38.60833615932423	40.0	38.0	41.0	34.0	41.0
16	38.53992662700003	40.0	38.0	41.0	34.0	41.0
17	38.45397539846471	40.0	38.0	41.0	34.0	41.0
18	38.26869315904677	40.0	38.0	41.0	34.0	41.0
19	38.048919443844994	40.0	37.0	41.0	34.0	41.0
20	38.01656441717791	40.0	36.0	41.0	34.0	41.0
21	37.96073619631902	40.0	36.0	41.0	33.0	41.0
22	37.97257144618799	40.0	36.0	41.0	34.0	41.0
23	37.89510127323735	40.0	36.0	41.0	34.0	41.0
24	37.83248759133089	40.0	36.0	41.0	33.0	41.0
25	37.72236951629312	40.0	35.0	41.0	33.0	41.0
26	37.61974288621019	40.0	35.0	41.0	33.0	41.0
27	37.52693837284583	39.0	35.0	41.0	33.0	41.0
28	37.49179640533958	40.0	35.0	41.0	33.0	41.0
29	37.44636680334186	40.0	35.0	41.0	33.0	41.0
30	37.33479668280051	39.0	35.0	41.0	33.0	41.0
31	37.17462157412831	39.0	35.0	41.0	32.0	41.0
32	36.94298178006597	39.0	35.0	41.0	31.0	41.0
33	36.707414372475874	39.0	35.0	41.0	31.0	41.0
34	36.412100379196595	39.0	35.0	41.0	30.0	41.0
35	36.17540154761538	39.0	35.0	41.0	29.0	41.0
36	35.94289545888954	39.0	35.0	41.0	27.0	41.0
37	35.82883435582822	39.0	35.0	41.0	27.0	41.0
38	35.65304436291889	39.0	35.0	41.0	25.0	41.0
39	35.56971359866819	39.0	35.0	41.0	25.0	41.0
40	35.41003483676049	39.0	35.0	40.0	24.0	41.0
41	35.302108703024324	39.0	35.0	40.0	24.0	41.0
42	35.205453648611154	39.0	35.0	40.0	23.0	41.0
43	35.16811973980331	39.0	35.0	40.0	23.0	41.0
44	34.97851835866449	38.0	34.0	40.0	23.0	41.0
45	34.87671486265684	38.0	34.0	40.0	23.0	41.0
46	34.75164780960015	38.0	34.0	40.0	23.0	41.0
47	34.63006134969325	38.0	34.0	40.0	22.0	41.0
48	34.558359281067915	38.0	34.0	40.0	22.0	41.0
49	34.4259703425101	38.0	34.0	40.0	21.0	41.0
50	34.23239202145698	38.0	33.0	40.0	20.0	41.0
51	32.33302093288528	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	21.0
10	22.0
11	12.0
12	12.0
13	10.0
14	12.0
15	18.0
16	35.0
17	73.0
18	124.0
19	234.0
20	383.0
21	658.0
22	1010.0
23	1745.0
24	2747.0
25	4117.0
26	5496.0
27	6061.0
28	5903.0
29	5831.0
30	6420.0
31	7613.0
32	9438.0
33	13065.0
34	19554.0
35	24998.0
36	29073.0
37	47671.0
38	62130.0
39	69859.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.47374911366649	17.596263526220056	22.070475074760303	20.859512285353148
2	34.363227178838976	23.716434935413265	25.512531985078766	16.40780590066899
3	30.52810062582853	23.31010882634029	30.753768844221106	15.408021703610075
4	26.240096186453744	24.361994019175633	31.64256867157875	17.755341122791872
5	24.38357431328421	28.38702716034158	29.871134815180195	17.35826371119401
6	25.377192712026392	32.48358356198169	28.716280790455347	13.422942935536577
7	58.37747017295064	27.305546135585907	10.895582205506058	3.4214014859573942
8	80.28393501248574	4.435058729228967	12.340845330949225	2.940160927336067
9	74.62558189721614	6.35385516539754	14.080833615932423	4.939729321453895
10	37.70601473625798	26.87979776181521	21.451428923759906	13.962758578166909
11	27.781237475722172	26.25890187131979	28.88029102568055	17.07956962727749
12	24.594136325800783	24.403921447729445	32.33961217128588	18.662330055183894
13	22.68212226778062	24.24176095199926	34.021950242007584	19.054166538212534
14	19.84801307149243	26.089650707525358	33.97108240589451	20.091253815087708
15	20.224435058729227	25.51931436322718	35.60501895982983	18.651231618213767
16	23.412461078398124	25.66606036316552	32.87079569627277	18.050682862163576
17	23.666491969047694	25.32015907759657	31.914788667262695	19.09856028609304
18	24.8494003761137	24.189351666306997	31.91941301600025	19.04183494157906
19	23.00921786848352	26.241329346117087	31.32133057927675	19.428122206122637
20	24.925547985325398	25.669451552239725	31.734439066498133	17.67056139593674
21	23.857015137034868	26.39670746369886	32.0436538520825	17.70262354718377
22	23.92144772944477	23.33847149859728	32.428707956962725	20.31137281499522
23	21.828467490828373	25.84055245552918	33.286062212905016	19.04491784073743
24	21.89074205382742	24.826895212257607	33.36745075068595	19.91491198322903
25	21.33119585658353	27.333908807842892	31.741221444646545	19.593673890927025
26	21.555014335481086	26.812898850078614	31.185991306224377	20.446095508215926
27	21.264913524678608	26.260751610814808	32.264389431821684	20.209945432684897
28	20.007090668064247	26.82399728704874	32.52828559977804	20.640626445108982
29	20.93473502481734	26.580448253537625	32.50917162499615	19.97564509664889
30	21.714708511884577	25.673459321145604	32.982396645805714	19.6294355211641
31	23.023399204612016	25.85874156056355	31.24333323057003	19.8745260042544
32	22.969756759256406	26.14144341338595	31.82970065049172	19.059099176865924
33	22.478650923328296	25.999938342016833	30.779665197151402	20.741745537503466
34	21.170885100348368	26.977833955051327	32.37475722169128	19.476523722909022
35	21.476708696858527	26.722878194654253	32.14970558313038	19.650707525356843
36	22.452446280482167	28.480439004840154	29.749976878256312	19.317137836421374
37	22.094521688195577	27.391867312020224	30.61133890310448	19.902272096679717
38	22.15895428060548	28.279433979714526	29.29154977340691	20.270061966273083
39	22.439189814101184	26.86931590467676	29.377562659925395	21.313931621296668
40	23.32367358263711	26.395474304035517	29.88562444122453	20.395227672102845
41	21.42522428091377	27.241730123007677	29.87298455467522	21.460061041403335
42	21.87409439837223	26.78576933748497	30.640009865277307	20.700126398865493
43	22.594876221598792	26.601103677898696	29.782347319419184	21.021672781083332
44	22.55695656195086	26.623300551838952	29.355982365816814	21.463760520393375
45	22.07787403274039	26.45682399728705	29.19197213059161	22.273329839380953
46	21.703918364830287	27.1967197952955	29.389585966643033	21.709775873231187
47	21.00101735672226	26.961494589511975	30.864444924006534	21.173043129759225
48	21.616672318648458	26.82985479544964	30.537041033387798	21.016431852514103
49	21.985078768073496	26.023060085704596	30.640934735024818	21.35092641119709
50	20.86598637358572	26.591546690507755	30.93072725591146	21.61173967999507
51	21.09288775164164	26.024601535283782	29.826432777383854	23.056077935690723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	239.5
2	416.0
3	4302.0
4	8188.0
5	5646.5
6	3105.0
7	2735.0
8	2365.0
9	2244.5
10	2124.0
11	2065.5
12	2007.0
13	1949.0
14	1891.0
15	1737.5
16	1584.0
17	1561.0
18	1538.0
19	1476.5
20	1415.0
21	1485.0
22	1555.0
23	1677.0
24	1799.0
25	2080.5
26	3022.0
27	3682.0
28	4221.5
29	4761.0
30	5629.0
31	6497.0
32	7361.0
33	8225.0
34	9357.0
35	10489.0
36	11334.0
37	12179.0
38	13201.0
39	14223.0
40	15221.0
41	16219.0
42	17599.0
43	18979.0
44	20211.0
45	21443.0
46	23730.5
47	26018.0
48	25120.5
49	24223.0
50	23897.5
51	23572.0
52	22406.0
53	21240.0
54	19683.5
55	18127.0
56	16783.0
57	15439.0
58	14114.5
59	12790.0
60	11553.5
61	10317.0
62	9169.0
63	8021.0
64	7067.5
65	6114.0
66	5098.5
67	4083.0
68	3483.0
69	2883.0
70	2331.5
71	1780.0
72	1438.5
73	1097.0
74	897.5
75	559.5
76	421.0
77	322.0
78	223.0
79	166.0
80	109.0
81	80.0
82	51.0
83	38.0
84	25.0
85	19.5
86	14.0
87	11.5
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	324370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.582529821115116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.17438168386627	33.80258312876384
2	11.864985329923813	11.765919778960818
3	6.55253104903823	9.746731984281624
4	4.076173219846748	8.084279209163288
5	2.7973932101965726	6.935091613297825
6	1.9996012367826548	5.948717277186879
7	1.3848251585193783	4.8064194303522285
8	0.9979687766519838	3.9585453303111007
9	0.6773749737656776	3.0227368353132404
>10	1.4530139383444853	8.924916929205583
>50	0.011807915377724682	0.4397160175889192
>100	0.008079099995285308	0.8091760772168898
>500	0.0012429384608131245	0.48193122039878733
>1k	6.214692304065622E-4	1.2732351679589446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4132	1.2738539322378764	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	785	0.2420075839319296	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	779	0.24015784443690846	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	461	0.1421216512007892	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.11745845793384099	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	369	0.11375897894379874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04069426889046459	0.0
2	0.0	0.0	0.0	0.18127447051206955	0.0
3	0.0	0.0	0.0	0.26050497888214075	0.0
4	0.0	0.0	0.0	0.4149582267164041	0.0
5	0.0	0.0	0.0	0.9741961340444554	0.0
6	0.0	0.0	0.0	1.3684989363997904	0.0
7	0.0	0.0	0.0	1.6706230539199063	0.0
8	0.0	0.0	0.0	2.1419983352344545	0.0
9	0.0	0.0	0.0	2.426241637636033	0.0
10	0.0	0.0	0.0	3.013225637389401	0.0
11	0.0	0.0	0.0	3.38070721706693	0.0
12	0.0	0.0	0.0	3.797515183278355	0.0
13	0.0	0.0	0.0	3.9581342294293553	0.0
14	0.0	0.0	0.0	4.059253321823843	0.0
15	0.0	0.0	0.0	4.179486389000216	0.0
16	0.0	0.0	0.0	4.353670191448038	0.0
17	0.0	0.0	0.0	4.549742577920276	0.0
18	0.0	0.0	0.0	4.774794216481179	0.0
19	0.0	0.0	0.0	4.951444338255696	0.0
20	0.0	0.0	0.0	5.115146283565065	0.0
21	0.0	0.0	0.0	5.305977741468077	0.0
22	0.0	0.0	0.0	5.521472392638037	0.0
23	0.0	0.0	0.0	5.720935968184481	0.0
24	0.0	0.0	0.0	5.904368468107408	0.0
25	0.0	0.0	0.0	6.072078182322656	0.0
26	0.0	0.0	0.0	6.247803434349662	0.0
27	0.0	0.0	0.0	6.418904337639116	0.0
28	0.0	0.0	0.0	6.604186577057065	0.0
29	0.0	0.0	0.0	6.795018034960076	0.0
30	0.0	0.0	0.0	7.041033387797885	0.0
31	0.0	0.0	0.0	7.243271572586861	0.0
32	0.0	0.0	0.0	7.464932022073558	0.0
33	0.0	0.0	0.0	7.694608009372013	0.0
34	0.0	0.0	0.0	7.921817677343774	0.0
35	0.0	0.0	0.0	8.156734593211455	0.0
36	0.0	0.0	0.0	8.363288836822148	0.0
37	0.0	0.0	0.0	8.585257576224683	0.0
38	0.0	0.0	0.0	8.849153744181027	0.0
39	0.0	0.0	0.0	9.171933286062213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGCG	45	3.8380676E-10	45.000004	5
ACACGAC	95	0.0	45.0	26
CACGACC	95	0.0	45.0	27
CGATGTA	25	3.884594E-5	45.0	10
CGTTTTT	1955	0.0	42.928387	1
TCACGGG	65	0.0	41.538464	3
GCGAGAC	105	0.0	40.714287	21
GACACGA	100	0.0	40.5	25
TAGGCGA	50	1.0768417E-9	40.5	6
ACGTGAG	80	0.0	39.375	44
CGTTTTA	350	0.0	39.214287	1
CACGGGA	75	0.0	39.0	4
CGGTTTT	145	0.0	38.793102	1
CGTCTCG	35	6.2344297E-6	38.57143	42
CGAGACA	105	0.0	38.57143	22
TCACGTC	35	6.2344297E-6	38.57143	39
ACAACGA	90	0.0	37.500004	13
CACAACG	90	0.0	37.500004	12
CGGGCAA	30	1.1381163E-4	37.499996	6
AGACACG	110	0.0	36.81818	24
>>END_MODULE