##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936530.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135349
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997369762613687	33.0	31.0	34.0	30.0	34.0
2	32.11740020243962	33.0	31.0	34.0	30.0	34.0
3	32.034163532793	33.0	31.0	34.0	30.0	34.0
4	35.77948858137112	37.0	35.0	37.0	35.0	37.0
5	35.83473095479095	37.0	35.0	37.0	35.0	37.0
6	35.79983597957872	37.0	35.0	37.0	35.0	37.0
7	26.759946508655403	35.0	10.0	37.0	0.0	37.0
8	31.10073956955722	35.0	19.0	37.0	17.0	37.0
9	35.908562309289316	39.0	32.0	39.0	32.0	39.0
10	36.65323718682813	38.0	35.0	39.0	33.0	39.0
11	37.013890017658056	39.0	37.0	39.0	34.0	39.0
12	37.03031422470798	39.0	37.0	39.0	33.0	39.0
13	36.96772787386682	39.0	37.0	39.0	33.0	39.0
14	38.14243178745318	40.0	37.0	41.0	33.0	41.0
15	38.22681364472586	40.0	37.0	41.0	33.0	41.0
16	38.18436043118161	40.0	37.0	41.0	33.0	41.0
17	38.0850098633902	40.0	37.0	41.0	33.0	41.0
18	37.98422596398939	40.0	37.0	41.0	33.0	41.0
19	37.891598755809056	40.0	36.0	41.0	33.0	41.0
20	37.95078648530835	40.0	36.0	41.0	33.0	41.0
21	37.90436575076284	40.0	36.0	41.0	33.0	41.0
22	37.89018020081419	40.0	36.0	41.0	33.0	41.0
23	37.85093351262292	40.0	36.0	41.0	33.0	41.0
24	37.82060451130042	40.0	36.0	41.0	33.0	41.0
25	37.737094474284994	40.0	36.0	41.0	33.0	41.0
26	37.57912507665369	40.0	36.0	41.0	33.0	41.0
27	37.4622863855662	39.0	36.0	41.0	33.0	41.0
28	37.42824106568944	39.0	36.0	41.0	33.0	41.0
29	37.40128113247974	39.0	36.0	41.0	33.0	41.0
30	37.325403216868985	39.0	36.0	41.0	32.0	41.0
31	37.159565272000535	39.0	35.0	41.0	32.0	41.0
32	37.07130455341377	39.0	35.0	41.0	31.0	41.0
33	36.998980413597444	39.0	35.0	41.0	31.0	41.0
34	36.7905710422685	39.0	35.0	41.0	31.0	41.0
35	36.742074193381555	39.0	35.0	41.0	31.0	41.0
36	36.53936120695388	39.0	35.0	41.0	30.0	41.0
37	36.43228246976335	39.0	35.0	41.0	30.0	41.0
38	36.323016793622415	39.0	35.0	40.0	30.0	41.0
39	36.21726056343231	39.0	35.0	40.0	30.0	41.0
40	36.014990875440525	38.0	35.0	40.0	29.0	41.0
41	35.91468721601194	38.0	35.0	40.0	29.0	41.0
42	35.80762325543595	38.0	35.0	40.0	28.0	41.0
43	35.770216255753645	38.0	35.0	40.0	28.0	41.0
44	35.53202461783981	38.0	34.0	40.0	27.0	41.0
45	35.39859178863531	38.0	34.0	40.0	27.0	41.0
46	35.1952729610119	38.0	34.0	40.0	26.0	41.0
47	35.05650577396213	38.0	34.0	40.0	25.0	41.0
48	34.99073506268979	38.0	34.0	40.0	25.0	41.0
49	34.77934820353309	37.0	34.0	40.0	24.0	41.0
50	34.55797974126148	37.0	34.0	40.0	24.0	41.0
51	32.23791088223777	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	11.0
11	7.0
12	4.0
13	5.0
14	10.0
15	11.0
16	14.0
17	46.0
18	60.0
19	97.0
20	140.0
21	219.0
22	342.0
23	504.0
24	700.0
25	988.0
26	1340.0
27	1662.0
28	1949.0
29	2338.0
30	2841.0
31	3676.0
32	4739.0
33	6421.0
34	9865.0
35	12526.0
36	12920.0
37	19823.0
38	25582.0
39	26491.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97223474129842	18.88229687696252	23.48299581082978	15.662472570909278
2	28.066701637987723	27.048592896881395	26.999091238206415	17.88561422692447
3	32.19159358399397	25.013114245395236	26.457528315687597	16.3377638549232
4	29.08407154836755	25.27613798402648	26.83654847837812	18.80324198922785
5	25.19634426556532	30.225565020798083	24.445692247449184	20.13239846618741
6	26.68656584090019	35.422500350944595	23.927771908178116	13.963161899977097
7	60.55382751257859	28.56689003982298	6.584459434498961	4.294823013099469
8	82.11512460380203	5.977879408048822	7.505042519708309	4.401953468440845
9	75.823242136994	7.5840974074429806	9.584851014784004	7.007809440779022
10	43.451373855735916	25.270966168941033	16.967247633894598	14.310412341428455
11	32.856541237837	26.37330161286748	22.881587599465085	17.88856954983044
12	30.4051008873357	23.51845968570141	27.0766684644881	18.999770962474788
13	24.716843124071843	25.904882932271388	29.0316145667866	20.346659376870164
14	19.769632579479715	29.241442493110405	28.44646063140474	22.542464296005143
15	19.273877162003412	26.204848207227244	34.66446002556354	19.856814605205802
16	21.81988784549572	25.033801505737017	31.63968703130426	21.506623617463003
17	21.779990986265137	24.674729772661784	28.70948437003598	24.8357948710371
18	23.313803574463055	26.33192709218391	28.393264819097297	21.961004514255738
19	25.182306481761962	26.77670318953225	26.113233197142204	21.927757131563588
20	26.7338510073957	25.801446630562474	27.75048208704904	19.714220274992797
21	25.547288860649136	26.662184426925943	26.887527798506085	20.902998913918832
22	23.386208985659295	25.540639384110708	26.278731279876467	24.79442035035353
23	23.05964580454972	27.190448396367906	27.007218376197827	22.74268742288454
24	23.140178353737376	24.504798705568568	28.89123672875308	23.46378621194098
25	22.11837545899859	26.96806034769374	27.742354949057624	23.17120924425005
26	21.453427805155563	28.19378052294439	27.650739938972578	22.70205173292747
27	22.818786987713242	27.842835927860566	26.91929751974525	22.419079564680935
28	20.252089043879156	27.645568123887138	29.66627016084345	22.43607267139026
29	21.680987668915176	26.069642184279157	28.68658061751472	23.562789529290946
30	21.757826064470372	26.56096461739651	27.146118552778375	24.53509076535475
31	23.61524651087189	28.51812721187449	24.678423926294247	23.188202350959372
32	24.025297564075096	27.727578334527774	25.245845924240296	23.00127817715683
33	22.21959526852803	27.143163229872403	24.372548005526454	26.264693496073114
34	20.96506069494418	28.194519353670884	27.075190803035117	23.765229148349825
35	20.772225875329703	26.845414447096026	26.488559206200268	25.893800471374
36	21.234733910113853	29.205239787512284	25.43941957458127	24.120606727792595
37	21.319699443660465	29.763056986013936	26.000930926715384	22.916312643610222
38	20.56461444118538	29.31975855011858	24.8069804727039	25.30864653599214
39	22.22033409925452	26.90821505884787	25.717958758468846	25.153492083428763
40	22.80031621955094	26.10288956697131	27.87608331055272	23.22071090292503
41	19.69574950683049	27.780035316108727	26.80403992641246	25.720175250648325
42	20.982792632379997	27.585722835041267	26.467871945858484	24.963612586720256
43	22.739732099978575	25.641120362913654	26.029006494322086	25.590141042785685
44	22.12502493553702	26.127270980945553	26.623026398421857	25.12467768509557
45	21.30196750622465	26.610466276071488	25.680278391417744	26.40728782628612
46	21.880471965068082	27.778557654655746	25.5317734153928	24.809196964883377
47	20.591212347339102	26.054865569749314	28.880893098582185	24.4730289843294
48	21.48002571130928	25.239196447701868	27.610843079741997	25.66993476124685
49	20.769270552423734	24.781860228003165	29.54879607533118	24.900073144241922
50	19.792536332000978	25.71130928193042	28.38809300401185	26.108061382056757
51	20.270559812041462	25.610089472400976	26.24917805081678	27.87017266474078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48.0
1	139.0
2	230.0
3	360.0
4	490.0
5	358.0
6	226.0
7	235.5
8	245.0
9	222.5
10	200.0
11	224.0
12	248.0
13	233.5
14	219.0
15	219.5
16	220.0
17	204.0
18	188.0
19	231.0
20	274.0
21	402.0
22	530.0
23	550.5
24	571.0
25	777.0
26	1097.0
27	1211.0
28	1505.5
29	1800.0
30	2154.0
31	2508.0
32	2766.5
33	3025.0
34	3268.0
35	3511.0
36	3871.0
37	4231.0
38	4543.5
39	4856.0
40	5521.5
41	6187.0
42	7112.0
43	8037.0
44	8879.0
45	9721.0
46	11045.5
47	12370.0
48	12212.5
49	12055.0
50	11744.5
51	11434.0
52	10512.0
53	9590.0
54	8804.0
55	8018.0
56	7337.5
57	6657.0
58	6528.5
59	6400.0
60	6159.5
61	5919.0
62	5227.0
63	4535.0
64	3764.0
65	2993.0
66	2575.0
67	2157.0
68	1686.5
69	1216.0
70	1020.5
71	825.0
72	713.0
73	601.0
74	484.5
75	280.5
76	193.0
77	155.0
78	117.0
79	80.0
80	43.0
81	39.0
82	35.0
83	33.5
84	32.0
85	23.0
86	14.0
87	11.5
88	9.0
89	6.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	135349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31844343142543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69435206380743	35.766056638763494
2	11.233965360860363	11.530192317638106
3	6.177745144617688	9.510967942134778
4	3.9822053297628814	8.17442315791029
5	2.7123914827452165	6.959785443557026
6	1.8442534444780376	5.678652963819459
7	1.402266085028578	5.037347893224183
8	0.9660375185361149	3.9660433398104162
9	0.6579420953368175	3.038810778062638
>10	1.295728415324148	8.419714959105718
>50	0.021595473588735804	0.7543461717485908
>100	0.011517585913992427	1.163658394225299
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	327	0.24159764756296687	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	271	0.20022312687940066	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	261	0.19283481961447813	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179	0.13225070004211334	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	172	0.12707888495666758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15072146820441967	0.0
2	0.0	0.0	0.0	0.6693806382019816	0.0
3	0.0	0.0	0.0	0.8762532416198124	0.0
4	0.0	0.0	0.0	1.177696178028652	0.0
5	0.0	0.0	0.0	2.2401347627245123	0.0
6	0.0	0.0	0.0	2.761010424901551	0.0
7	0.0	0.0	0.0	3.289274394343512	0.0
8	0.0	0.0	0.0	4.316249104167744	0.0
9	0.0	0.0	0.0	4.704874066302669	0.0
10	0.0	0.0	0.0	5.861882983989538	0.0
11	0.0	0.0	0.0	6.833445389326851	0.0
12	0.0	0.0	0.0	7.950557447783138	0.0
13	0.0	0.0	0.0	8.30889035013188	0.0
14	0.0	0.0	0.0	8.449268188165409	0.0
15	0.0	0.0	0.0	8.723374387694035	0.0
16	0.0	0.0	0.0	9.238339404059136	0.0
17	0.0	0.0	0.0	9.811672047817124	0.0
18	0.0	0.0	0.0	10.469970225121722	0.0
19	0.0	0.0	0.0	10.854162202897694	0.0
20	0.0	0.0	0.0	11.25904144101545	0.0
21	0.0	0.0	0.0	11.757013350671228	0.0
22	0.0	0.0	0.0	12.30227042682251	0.0
23	0.0	0.0	0.0	12.785465721948444	0.0
24	0.0	0.0	0.0	13.18738963716023	0.0
25	0.0	0.0	0.0	13.581925245107094	0.0
26	0.0	0.0	0.0	13.93139217873793	0.0
27	0.0	0.0	0.0	14.2394845916852	0.0
28	0.0	0.0	0.0	14.56087595770933	0.0
29	0.0	0.0	0.0	14.930291320955456	0.0
30	0.0	0.0	0.0	15.360290803773948	0.0
31	0.0	0.0	0.0	15.710496568131276	0.0
32	0.0	0.0	0.0	16.070307131933003	0.0
33	0.0	0.0	0.0	16.425684711375776	0.0
34	0.0	0.0	0.0	16.775890475733103	0.0
35	0.0	0.0	0.0	17.150477654064677	0.0
36	0.0	0.0	0.0	17.536147293293634	0.0
37	0.0	0.0	0.0	17.89817434927484	0.0
38	0.0	0.0	0.0	18.209222085128076	0.0
39	0.0	0.0	0.0	18.577159786921218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCGC	25	3.8725226E-5	45.000004	17
AGCGACT	25	3.8725226E-5	45.000004	19
ACGAGCG	25	3.8725226E-5	45.000004	16
ACTATGC	20	7.0112164E-4	45.0	8
GCCCTCG	20	7.0112164E-4	45.0	13
TGCGTAG	20	7.0112164E-4	45.0	2
ACTTATA	50	1.0659278E-9	40.500004	7
ACAAGGA	35	6.2075214E-6	38.571426	4
ACAACGA	30	1.1346071E-4	37.499996	13
ACACAAC	30	1.1346071E-4	37.499996	11
AGGGTCC	30	1.1346071E-4	37.499996	7
TAACGGG	30	1.1346071E-4	37.499996	3
TGCATGG	30	1.1346071E-4	37.499996	2
CTTGCGG	30	1.1346071E-4	37.499996	2
CGCTAGG	30	1.1346071E-4	37.499996	2
GAGCGAC	30	1.1346071E-4	37.499996	18
CGGGCTC	30	1.1346071E-4	37.499996	6
AACGAGC	30	1.1346071E-4	37.499996	15
ATACGAA	30	1.1346071E-4	37.499996	19
CACAACG	30	1.1346071E-4	37.499996	12
>>END_MODULE