##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936529.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95125
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.152	33.0	31.0	34.0	30.0	34.0
2	32.280252299605785	33.0	31.0	34.0	30.0	34.0
3	32.202123521682	33.0	31.0	34.0	30.0	34.0
4	35.91754007884363	37.0	35.0	37.0	35.0	37.0
5	35.96582391590013	37.0	35.0	37.0	35.0	37.0
6	35.94164520367937	37.0	35.0	37.0	35.0	37.0
7	26.817818659658343	36.0	10.0	37.0	0.0	37.0
8	31.204951379763468	37.0	19.0	37.0	17.0	37.0
9	36.05446517739816	39.0	32.0	39.0	32.0	39.0
10	36.82963469119579	38.0	37.0	39.0	34.0	39.0
11	37.19067542706964	39.0	37.0	39.0	34.0	39.0
12	37.13510643889619	39.0	37.0	39.0	34.0	39.0
13	37.02402102496715	39.0	37.0	39.0	33.0	39.0
14	38.218890932982916	40.0	37.0	41.0	33.0	41.0
15	38.32725361366623	40.0	37.0	41.0	34.0	41.0
16	38.297177398160315	40.0	37.0	41.0	34.0	41.0
17	38.227752956636	40.0	37.0	41.0	34.0	41.0
18	38.13613666228647	40.0	37.0	41.0	34.0	41.0
19	38.02407358738502	40.0	37.0	41.0	34.0	41.0
20	38.061119579500655	40.0	36.0	41.0	34.0	41.0
21	38.01915374507227	40.0	36.0	41.0	34.0	41.0
22	38.02240210249671	40.0	36.0	41.0	34.0	41.0
23	37.928777923784494	40.0	36.0	41.0	34.0	41.0
24	37.861371879106436	40.0	36.0	41.0	34.0	41.0
25	37.76608672798949	40.0	36.0	41.0	33.0	41.0
26	37.64347963206308	40.0	35.0	41.0	33.0	41.0
27	37.55080157687254	40.0	35.0	41.0	33.0	41.0
28	37.530270696452035	40.0	35.0	41.0	33.0	41.0
29	37.54504073587385	40.0	36.0	41.0	33.0	41.0
30	37.47020236530881	40.0	36.0	41.0	33.0	41.0
31	37.316846254927725	39.0	35.0	41.0	33.0	41.0
32	37.15973718791064	39.0	35.0	41.0	32.0	41.0
33	37.06255978975033	39.0	35.0	41.0	32.0	41.0
34	36.931574244415245	39.0	35.0	41.0	31.0	41.0
35	36.74620762155059	39.0	35.0	41.0	31.0	41.0
36	36.58659658344284	39.0	35.0	41.0	31.0	41.0
37	36.5433061760841	39.0	35.0	41.0	30.0	41.0
38	36.373266754270695	39.0	35.0	41.0	30.0	41.0
39	36.36599211563732	39.0	35.0	41.0	30.0	41.0
40	36.13299342969776	39.0	35.0	40.0	30.0	41.0
41	36.00946123521682	39.0	35.0	40.0	29.0	41.0
42	35.92091458607096	38.0	35.0	40.0	29.0	41.0
43	35.871148488830485	38.0	35.0	40.0	29.0	41.0
44	35.67447043363995	38.0	35.0	40.0	28.0	41.0
45	35.56743232588699	38.0	35.0	40.0	28.0	41.0
46	35.40541392904073	38.0	34.0	40.0	27.0	41.0
47	35.28411038107753	38.0	34.0	40.0	26.0	41.0
48	35.15503810775296	38.0	34.0	40.0	26.0	41.0
49	35.02198160315375	38.0	34.0	40.0	26.0	41.0
50	34.80721156373193	37.0	34.0	40.0	25.0	41.0
51	32.74087779237845	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	13.0
10	14.0
11	3.0
12	2.0
13	2.0
14	2.0
15	9.0
16	9.0
17	30.0
18	52.0
19	84.0
20	110.0
21	158.0
22	245.0
23	341.0
24	494.0
25	674.0
26	889.0
27	1044.0
28	1235.0
29	1428.0
30	1748.0
31	2228.0
32	2896.0
33	4214.0
34	7255.0
35	9148.0
36	8713.0
37	14081.0
38	18077.0
39	19918.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57161629434954	19.024441524310117	23.085413929040737	16.318528252299604
2	28.536136662286467	26.57030223390276	26.815243101182656	18.078318002628123
3	31.91064388961892	25.10275952693824	26.47568988173456	16.51090670170828
4	30.013140604467804	26.08988173455979	25.58318002628121	18.313797634691195
5	26.371616294349543	30.355847568988175	24.116688567674114	19.155847568988175
6	26.93718791064389	34.960315374507225	23.77082785808147	14.33166885676741
7	60.57398160315375	29.014454664914584	6.395795006570302	4.015768725361367
8	82.86044678055191	6.323258869908016	7.269382391590014	3.5469119579500656
9	76.257555847569	8.846254927726676	9.277266754270697	5.61892247043364
10	44.74323258869908	26.211826544021026	15.890670170827859	13.154270696452036
11	36.173455978975035	25.097503285151117	21.904862023653088	16.82417871222076
12	33.50328515111695	22.529303547963206	25.4580814717477	18.50932982917214
13	25.184756898817344	27.57634691195795	27.221024967148487	20.017871222076216
14	20.8	30.761629434954006	27.769776609724044	20.668593955321946
15	20.39106438896189	25.09119579500657	35.197897503285155	19.319842312746385
16	22.855190538764784	24.372141918528254	33.55900131406045	19.213666228646517
17	22.80893561103811	24.34796320630749	27.73929040735874	25.103810775295663
18	24.871484888304863	25.194218134034163	28.146123521682	21.788173455978978
19	27.452299605781867	26.61130091984231	25.351905387647832	20.58449408672799
20	29.236268068331146	25.12168199737188	26.06990801576873	19.572141918528253
21	26.402102496714846	27.374507227332458	25.593692509855455	20.62969776609724
22	24.724310118265443	25.122733245729307	26.574507227332454	23.5784494086728
23	24.44467805519054	27.769776609724044	25.91222076215506	21.873324572930354
24	23.85703022339028	25.351905387647832	28.711695137976346	22.079369250985543
25	22.514586070959265	26.26754270696452	28.194480946123523	23.023390275952693
26	21.463337713534823	29.595795006570302	26.783705650459922	22.157161629434956
27	22.540867279894876	28.798948751642577	27.338764783180025	21.321419185282522
28	20.679106438896188	28.37634691195795	29.653613666228644	21.290932982917212
29	21.745072273324574	27.717214191852825	28.39947437582129	22.138239159001312
30	23.693035479632062	27.191590013140605	27.527989487516425	21.587385019710904
31	25.582128777923785	27.382917214191853	24.986070959264126	22.048883048620237
32	26.816294349540076	28.17030223390276	24.439421813403417	20.573981603153747
33	25.52746386333771	28.514060446780555	24.508804204993428	21.449671484888306
34	23.709855453350855	27.940078843626807	26.548226018396846	21.80183968462549
35	23.303022339027596	26.56189224704336	27.533245729303545	22.601839684625492
36	24.65282522996058	29.369776609724045	25.799737187910644	20.17766097240473
37	24.632851511169516	29.003942181340342	26.720630749014457	19.642575558475688
38	23.22312746386334	28.65703022339028	25.243626806833113	22.876215505913272
39	24.86622864651774	26.836268068331144	26.43574244415243	21.861760840998688
40	24.75900131406045	25.987910643889617	27.435479632063075	21.81760840998686
41	20.604467805519054	27.331406044678054	27.743495400788436	24.320630749014455
42	22.263337713534824	26.701708278580817	27.58160315374507	23.45335085413929
43	24.37109067017083	26.253876478318	26.65019710906702	22.724835742444153
44	22.81629434954008	27.440735873850198	27.05072273324573	22.692247043363995
45	22.977135348226017	27.771879106438895	25.255190538764783	23.995795006570305
46	22.94664914586071	29.078580814717476	25.3203679369251	22.654402102496714
47	23.11695137976347	26.786859395532193	28.172404730617608	21.92378449408673
48	23.376609724047306	25.34980289093298	28.5676741130092	22.705913272010513
49	22.230749014454666	25.598948751642574	29.133245729303546	23.037056504599214
50	20.630749014454665	26.730091984231276	28.74638633377135	23.892772667542708
51	21.248883048620236	27.087516425755588	26.562943495400788	25.10065703022339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	128.0
2	236.0
3	355.5
4	475.0
5	352.5
6	230.0
7	209.0
8	188.0
9	191.5
10	195.0
11	192.0
12	189.0
13	189.0
14	189.0
15	236.0
16	283.0
17	257.5
18	232.0
19	238.5
20	245.0
21	294.0
22	343.0
23	409.5
24	476.0
25	588.5
26	805.0
27	909.0
28	1139.0
29	1369.0
30	1588.0
31	1807.0
32	2065.0
33	2323.0
34	2495.5
35	2668.0
36	2821.0
37	2974.0
38	3307.0
39	3640.0
40	4145.5
41	4651.0
42	5292.0
43	5933.0
44	6358.5
45	6784.0
46	7022.0
47	7260.0
48	7259.0
49	7258.0
50	7150.5
51	7043.0
52	6569.0
53	6095.0
54	5668.0
55	5241.0
56	5123.0
57	5005.0
58	4825.5
59	4646.0
60	4581.0
61	4516.0
62	4203.5
63	3891.0
64	3256.5
65	2622.0
66	2063.0
67	1504.0
68	1266.5
69	1029.0
70	924.5
71	820.0
72	645.5
73	471.0
74	382.5
75	230.5
76	167.0
77	130.5
78	94.0
79	70.5
80	47.0
81	38.5
82	30.0
83	23.5
84	17.0
85	11.5
86	6.0
87	4.0
88	2.0
89	3.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	95125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.852036793692506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.53652845090649	31.17371879106439
2	11.3669000179501	10.651248357424443
3	6.607880093340514	9.28777923784494
4	4.314754981152396	8.086202365308806
5	3.006641536528451	7.043363994743758
6	2.234787291330102	6.282260183968462
7	1.5908274995512475	5.217345597897503
8	1.2609944354693952	4.726412614980289
9	0.8616047388260636	3.6331143232588703
>10	2.1966433315383234	12.997634691195795
>50	0.01795009872554299	0.5392904073587385
>100	0.004487524681385748	0.36162943495400784
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	178	0.1871222076215506	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	166	0.1745072273324573	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010512483574244415	0.0	0.0	0.05045992115637319	0.0
2	0.0010512483574244415	0.0	0.0	0.3069645203679369	0.0
3	0.0010512483574244415	0.0	0.0	0.4078843626806833	0.0
4	0.0010512483574244415	0.0	0.0	0.5613666228646518	0.0
5	0.0010512483574244415	0.0	0.0	1.0102496714848883	0.0
6	0.0010512483574244415	0.0	0.0	1.3151116951379764	0.0
7	0.0010512483574244415	0.0	0.0	1.6052562417871221	0.0
8	0.0010512483574244415	0.0	0.0	2.1624178712220763	0.0
9	0.0010512483574244415	0.0	0.0	2.4	0.0
10	0.0010512483574244415	0.0	0.0	2.949802890932983	0.0
11	0.0010512483574244415	0.0	0.0	3.6415243101182653	0.0
12	0.0010512483574244415	0.0	0.0	4.236530880420499	0.0
13	0.0010512483574244415	0.0	0.0	4.491984231274639	0.0
14	0.0010512483574244415	0.0	0.0	4.5739816031537455	0.0
15	0.0010512483574244415	0.0	0.0	4.756898817345598	0.0
16	0.0010512483574244415	0.0	0.0	5.104862023653088	0.0
17	0.0010512483574244415	0.0	0.0	5.503285151116952	0.0
18	0.0010512483574244415	0.0	0.0	5.991064388961893	0.0
19	0.0010512483574244415	0.0	0.0	6.265440210249672	0.0
20	0.0010512483574244415	0.0	0.0	6.514586070959264	0.0
21	0.0010512483574244415	0.0	0.0	6.841524310118265	0.0
22	0.0010512483574244415	0.0	0.0	7.174770039421813	0.0
23	0.0010512483574244415	0.0	0.0	7.496452036793692	0.0
24	0.0010512483574244415	0.0	0.0	7.7750328515111695	0.0
25	0.0010512483574244415	0.0	0.0	7.988436268068331	0.0
26	0.0010512483574244415	0.0	0.0	8.215505913272011	0.0
27	0.0010512483574244415	0.0	0.0	8.417345597897503	0.0
28	0.0010512483574244415	0.0	0.0	8.651773981603153	0.0
29	0.0010512483574244415	0.0	0.0	8.905124835742445	0.0
30	0.0010512483574244415	0.0	0.0	9.182654402102497	0.0
31	0.0010512483574244415	0.0	0.0	9.462286465177398	0.0
32	0.0010512483574244415	0.0	0.0	9.684099868593956	0.0
33	0.0010512483574244415	0.0	0.0	9.914323258869908	0.0
34	0.0010512483574244415	0.0	0.0	10.182391590013141	0.0
35	0.0010512483574244415	0.0	0.0	10.472536136662287	0.0
36	0.0010512483574244415	0.0	0.0	10.734296977660973	0.0
37	0.0010512483574244415	0.0	0.0	11.052825229960579	0.0
38	0.0010512483574244415	0.0	0.0	11.32089356110381	0.0
39	0.0010512483574244415	0.0	0.0	11.629960578186596	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGA	30	2.1444903E-6	45.000004	25
TCACATA	25	3.863786E-5	45.000004	33
TTAGCGG	25	3.863786E-5	45.000004	2
GGCAAAT	25	3.863786E-5	45.000004	8
TACGGTT	25	3.863786E-5	45.000004	33
TGTGCGG	25	3.863786E-5	45.000004	2
GACCCAC	25	3.863786E-5	45.000004	12
ACGGGAG	25	3.863786E-5	45.000004	5
ACCTATG	30	2.1444903E-6	45.000004	34
CTACGAA	30	2.1444903E-6	45.000004	11
TACGAAT	30	2.1444903E-6	45.000004	12
CTCATGC	25	3.863786E-5	45.000004	23
CGAATAT	30	2.1444903E-6	45.000004	14
GGCGATA	30	2.1444903E-6	45.000004	8
TATACTG	25	3.863786E-5	45.000004	18
CCTATGC	30	2.1444903E-6	45.000004	35
ACATAGT	25	3.863786E-5	45.000004	35
ATGCATA	25	3.863786E-5	45.000004	26
GACCTAT	30	2.1444903E-6	45.000004	33
GCATATC	25	3.863786E-5	45.000004	28
>>END_MODULE