##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936528.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281352
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01258921209019	33.0	31.0	34.0	30.0	34.0
2	32.124754755608635	33.0	31.0	34.0	30.0	34.0
3	32.04301728795246	33.0	31.0	34.0	30.0	34.0
4	35.777794364354975	37.0	35.0	37.0	35.0	37.0
5	35.843267508316984	37.0	35.0	37.0	35.0	37.0
6	35.79706915180983	37.0	35.0	37.0	35.0	37.0
7	26.730607921749268	35.0	10.0	37.0	0.0	37.0
8	31.042459268105432	35.0	19.0	37.0	17.0	37.0
9	35.88948363615684	39.0	32.0	39.0	32.0	39.0
10	36.660293866757655	38.0	35.0	39.0	33.0	39.0
11	37.004119394921666	39.0	37.0	39.0	34.0	39.0
12	37.01886960106912	39.0	37.0	39.0	33.0	39.0
13	36.95969461741875	39.0	37.0	39.0	33.0	39.0
14	38.09850649719924	40.0	37.0	41.0	33.0	41.0
15	38.15131934374023	40.0	37.0	41.0	33.0	41.0
16	38.13211919588274	40.0	37.0	41.0	33.0	41.0
17	38.006546958969544	40.0	37.0	41.0	33.0	41.0
18	37.887909096078936	40.0	36.0	41.0	33.0	41.0
19	37.76368748045154	40.0	36.0	41.0	33.0	41.0
20	37.87014842617077	40.0	36.0	41.0	33.0	41.0
21	37.819333077426144	40.0	36.0	41.0	33.0	41.0
22	37.87064246922005	40.0	36.0	41.0	33.0	41.0
23	37.8661392135119	40.0	36.0	41.0	33.0	41.0
24	37.77943643549717	40.0	36.0	41.0	33.0	41.0
25	37.69544556285365	40.0	36.0	41.0	33.0	41.0
26	37.55429497568882	39.0	36.0	41.0	33.0	41.0
27	37.469159629218915	39.0	36.0	41.0	33.0	41.0
28	37.404432881230626	39.0	36.0	41.0	32.0	41.0
29	37.34970783929028	39.0	36.0	41.0	32.0	41.0
30	37.22784270237994	39.0	35.0	41.0	32.0	41.0
31	37.123301060593136	39.0	35.0	41.0	31.0	41.0
32	36.91133526685433	39.0	35.0	41.0	31.0	41.0
33	36.829224601211294	39.0	35.0	41.0	31.0	41.0
34	36.62086994227871	39.0	35.0	41.0	30.0	41.0
35	36.53406409053428	39.0	35.0	41.0	30.0	41.0
36	36.35252281839119	39.0	35.0	41.0	30.0	41.0
37	36.28835409024994	39.0	35.0	41.0	30.0	41.0
38	36.07642739344309	39.0	35.0	40.0	29.0	41.0
39	35.92650843072024	39.0	35.0	40.0	29.0	41.0
40	35.740286189541926	38.0	35.0	40.0	27.0	41.0
41	35.5663617105974	38.0	35.0	40.0	27.0	41.0
42	35.5522583809605	38.0	35.0	40.0	27.0	41.0
43	35.434469987773326	38.0	34.0	40.0	26.0	41.0
44	35.25832053797378	38.0	34.0	40.0	26.0	41.0
45	35.172460121129404	38.0	34.0	40.0	26.0	41.0
46	34.91026543262532	38.0	34.0	40.0	24.0	41.0
47	34.77719724757599	38.0	34.0	40.0	24.0	41.0
48	34.65911385026586	37.0	34.0	40.0	23.0	41.0
49	34.47850379595667	37.0	33.0	40.0	24.0	41.0
50	34.30295857146919	37.0	33.0	40.0	23.0	41.0
51	32.01313656913759	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	21.0
9	20.0
10	29.0
11	17.0
12	6.0
13	6.0
14	13.0
15	18.0
16	31.0
17	60.0
18	126.0
19	188.0
20	373.0
21	561.0
22	819.0
23	1179.0
24	1715.0
25	2416.0
26	3320.0
27	3886.0
28	4580.0
29	5220.0
30	6324.0
31	7686.0
32	9862.0
33	13367.0
34	20080.0
35	24252.0
36	27053.0
37	41834.0
38	52052.0
39	54219.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04198299638887	18.035770138474224	22.858198982058063	15.06404788307885
2	28.12313401006568	27.285748812874978	26.1483835195769	18.442733657482442
3	31.541272143080555	26.647047115357275	25.936549233700134	15.875131507862037
4	27.79116551508431	25.055091131394125	28.216611220108618	18.93713213341295
5	26.707114219909577	28.915024595524468	24.409991754101622	19.96786943046433
6	26.166154852284684	34.87979470556456	25.071085330831128	13.882965111319628
7	60.21745002701242	28.126688276607236	7.6089738121641215	4.046887884216213
8	80.78741220961643	6.462367425858	8.416503170405756	4.333717194119822
9	75.17984588700276	6.896343370582046	9.728738377548408	8.195072364866785
10	42.34553157610396	24.944908868605875	17.417683186897552	15.291876368392618
11	31.57183883533794	24.68651369103472	23.960732463248885	19.780915010378457
12	28.18782166112201	23.129389519178822	28.388282294065796	20.29450652563337
13	24.379780488498394	24.658079558702266	30.85529870056015	20.10684125223919
14	20.655975432909663	27.143933577866868	30.832551394694192	21.367539594529273
15	19.15358696579374	24.71636952998379	35.35322300889988	20.776820495322585
16	23.710867525377463	25.508615542096734	29.141075947567458	21.639440984958345
17	24.342816116466206	24.779990901077653	27.973499388666156	22.90369359378999
18	26.14127498649379	23.8075435753078	28.721672495663796	21.32950894253462
19	24.719212943217038	26.727373539196453	25.91700076772157	22.636412749864938
20	27.01100400921266	26.93316557195257	27.26477864027979	18.791051778554976
21	25.520700048338025	27.483366032585515	27.561204469845602	19.434729449230854
22	24.911143336461087	23.67177059342034	27.93831214990475	23.478773920213825
23	23.260541954562257	26.41459808353948	28.819059398902443	21.50580056299582
24	23.922346384600075	23.9397622906537	28.57488128749751	23.563010037248713
25	23.058659615001847	27.78903295515937	26.550015638772784	22.602291791065994
26	22.850735022320794	25.29393784298672	27.081378486735474	24.77394864795701
27	23.067545281355738	26.092226108220306	27.237055361255653	23.603173249168304
28	19.929838778469673	26.183215331684153	28.778896186982855	25.108049702863315
29	22.72846825329125	25.69414825556598	28.154766982285533	23.42261650885723
30	22.651340669339476	24.510577497227672	28.91573544883278	23.922346384600075
31	23.565142597173647	24.72880946287924	26.68578862066024	25.02025931928687
32	24.912565043077713	26.55321447866018	26.402869003952347	22.131351474309763
33	23.711578378685775	24.681182291222385	26.776066990815778	24.831172339276065
34	21.735761608234526	25.230671898547012	29.406935084875883	23.626631408342575
35	20.772199948818564	26.876652733941825	27.710483664590974	24.64066365264864
36	22.358113679661066	26.77393443089084	28.094344451079074	22.773607438369016
37	21.580440160368504	27.387400835963486	26.585913730842503	24.4462452728255
38	21.088885097671245	27.778725582188855	26.37692285819898	24.755466461940916
39	22.961628138417357	25.753504506809975	26.79703716341096	24.487830191361713
40	23.673903153345275	24.566024055275953	28.302979897068443	23.457092894310332
41	20.637137820239417	25.374619693480057	27.598524268531943	26.389718217748587
42	22.0300548778754	24.345304103045294	28.869529983792546	24.75511103528676
43	22.56603827234212	23.96606386306122	28.21412323352953	25.253774631067134
44	21.976030026443745	24.75084591543689	28.073374278483893	25.19974977963547
45	21.804358952486567	24.12422872416048	26.958756291051778	27.112656032301174
46	22.895518780744407	25.626972617930566	26.268162302027353	25.209346299297675
47	20.056370667349086	25.255907190992065	30.508046859450083	24.179675282208763
48	21.9419090676448	25.726847507748303	28.069109158634024	24.262134265972872
49	22.26961244277631	23.4709545338224	30.10748102021667	24.151952003184622
50	20.293084819016748	25.701256788649097	29.188703119224318	24.81695527310984
51	20.596263755011517	24.262489692627028	27.35967755693935	27.781568995422106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	293.0
2	469.0
3	911.0
4	1353.0
5	949.5
6	546.0
7	508.5
8	471.0
9	470.5
10	470.0
11	472.0
12	474.0
13	492.5
14	511.0
15	513.5
16	516.0
17	569.5
18	623.0
19	675.5
20	728.0
21	858.5
22	989.0
23	1127.0
24	1265.0
25	1566.0
26	2098.5
27	2330.0
28	2789.0
29	3248.0
30	3989.0
31	4730.0
32	5445.5
33	6161.0
34	6953.5
35	7746.0
36	8148.0
37	8550.0
38	9454.5
39	10359.0
40	11796.5
41	13234.0
42	14205.0
43	15176.0
44	16868.0
45	18560.0
46	22651.5
47	26743.0
48	24506.5
49	22270.0
50	22251.5
51	22233.0
52	20851.5
53	19470.0
54	18202.5
55	16935.0
56	16009.5
57	15084.0
58	14620.0
59	14156.0
60	13151.5
61	12147.0
62	11091.5
63	10036.0
64	8833.5
65	7631.0
66	6256.0
67	4881.0
68	4161.5
69	3442.0
70	2924.0
71	2406.0
72	1908.5
73	1411.0
74	1180.5
75	738.0
76	526.0
77	389.0
78	252.0
79	184.0
80	116.0
81	97.5
82	79.0
83	60.0
84	41.0
85	33.5
86	26.0
87	21.0
88	16.0
89	10.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	281352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.82739786007768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.05671808447836	30.95632976484133
2	10.171156114140151	9.118929236510443
3	5.456312378920587	7.337768575762202
4	3.712784289861657	6.657378341210977
5	2.774745358393312	6.219230707055042
6	2.0185795899660577	5.429260223498456
7	1.578645465654182	4.953659785822132
8	1.2404947011023892	4.448651960770795
9	0.9806746216501905	3.9564982292394815
>10	2.9767269655947004	17.41359755421253
>50	0.016581215119208997	0.5252601938543041
>100	0.013422888429835857	1.3163360248949845
>500	7.895816723432857E-4	0.1886547731296119
>1k	0.002368745017029857	1.4784446291977278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	1486	0.5281640080752936	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC	1428	0.507549262134266	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	1263	0.4489038641985839	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.18944240666496062	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTT	439	0.15603230117432967	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	343	0.12191134237538742	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCT	319	0.11338110267565184	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTC	313	0.11124854275071797	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCT	299	0.10627256959253888	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08743495692228952	0.0
2	0.0	0.0	0.0	0.48480195626830447	0.0
3	0.0	0.0	0.0	0.5978276322898006	0.0
4	0.0	0.0	0.0	0.9059825414427479	0.0
5	0.0	0.0	0.0	2.1833859364781483	0.0
6	0.0	0.0	0.0	2.5736444027410506	0.0
7	0.0	0.0	0.0	3.05311495919702	0.0
8	0.0	0.0	0.0	3.852114077738918	0.0
9	0.0	0.0	0.0	4.100201882339561	0.0
10	0.0	0.0	0.0	5.409949102903125	0.0
11	0.0	0.0	0.0	6.112272171514686	0.0
12	0.0	0.0	0.0	7.341692968239074	0.0
13	0.0	0.0	0.0	7.61643777190139	0.0
14	0.0	0.0	0.0	7.76180727345105	0.0
15	0.0	0.0	0.0	8.039040063692456	0.0
16	0.0	0.0	0.0	8.43889504961756	0.0
17	0.0	0.0	0.0	8.866473314566806	0.0
18	0.0	0.0	0.0	9.34310045778953	0.0
19	0.0	0.0	0.0	9.659785606642213	0.0
20	0.0	0.0	0.0	9.975049048878274	0.0
21	0.0	0.0	0.0	10.380946287924024	0.0
22	0.0	0.0	0.0	10.803193153060935	0.0
23	0.0	0.0	0.0	11.19558417924877	0.0
24	0.0	0.0	0.0	11.497696835281072	0.0
25	0.0	0.0	0.0	11.786303278455458	0.0
26	0.0	0.0	0.0	12.060337228809463	0.0
27	0.0	0.0	0.0	12.36849213796241	0.0
28	0.0	0.0	0.0	12.65354431459524	0.0
29	0.0	0.0	0.0	12.960277517131566	0.0
30	0.0	0.0	0.0	13.369373596064715	0.0
31	0.0	0.0	0.0	13.697787824504536	0.0
32	0.0	0.0	0.0	14.037575705877336	0.0
33	0.0	0.0	0.0	14.369188774204556	0.0
34	0.0	0.0	0.0	14.701512695840087	0.0
35	0.0	0.0	0.0	15.066535869657937	0.0
36	0.0	0.0	0.0	15.400992351218402	0.0
37	0.0	0.0	0.0	15.727629446387443	0.0
38	0.0	0.0	0.0	16.117887912650346	0.0
39	0.0	0.0	0.0	16.706829878586255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	20	7.0241943E-4	45.000004	1
CGGGAAC	20	7.0241943E-4	45.000004	6
ACATACG	20	7.0241943E-4	45.000004	17
TGTCGTG	20	7.0241943E-4	45.000004	27
CGGCTAG	35	1.2075543E-7	45.0	2
ACAACGA	135	0.0	41.666668	13
CATATCA	60	3.6379788E-12	41.250004	29
ACACGAC	110	0.0	40.909092	26
TCAAGCG	110	0.0	40.909092	17
TGGTCAA	110	0.0	40.909092	14
CACAACG	140	0.0	40.17857	12
GGTAAAT	40	3.4458208E-7	39.375004	8
TGTACGG	40	3.4458208E-7	39.375004	2
AACGAGC	145	0.0	38.793102	15
TGATACC	205	0.0	38.414635	4
TTACACG	345	0.0	37.826088	34
ACACGCG	340	0.0	37.72059	36
CACGACC	120	0.0	37.500004	27
AACGGGA	60	1.546141E-10	37.500004	4
AACGGCT	30	1.13773174E-4	37.500004	7
>>END_MODULE