##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936526.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105562
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.025700536177794	33.0	31.0	34.0	30.0	34.0
2	32.13404444781266	33.0	31.0	34.0	30.0	34.0
3	32.07206191621985	33.0	31.0	34.0	30.0	34.0
4	35.7885886966901	37.0	35.0	37.0	35.0	37.0
5	35.853204751709896	37.0	35.0	37.0	35.0	37.0
6	35.84573047119228	37.0	35.0	37.0	35.0	37.0
7	26.75109414372596	36.0	10.0	37.0	0.0	37.0
8	31.144284875239197	36.0	19.0	37.0	17.0	37.0
9	35.979774919004946	39.0	32.0	39.0	32.0	39.0
10	36.726104090487105	38.0	35.0	39.0	33.0	39.0
11	37.0625224986264	39.0	37.0	39.0	34.0	39.0
12	36.99692124059794	39.0	35.0	39.0	34.0	39.0
13	36.82657585115856	39.0	35.0	39.0	33.0	39.0
14	37.958763570224136	40.0	37.0	41.0	33.0	41.0
15	38.118328565203385	40.0	37.0	41.0	33.0	41.0
16	38.08900930258995	40.0	36.0	41.0	33.0	41.0
17	37.99483715731039	40.0	36.0	41.0	33.0	41.0
18	37.925939258445275	40.0	36.0	41.0	33.0	41.0
19	37.80932532540118	40.0	36.0	41.0	33.0	41.0
20	37.85799814327125	40.0	36.0	41.0	33.0	41.0
21	37.80327201076145	40.0	36.0	41.0	33.0	41.0
22	37.78807714897406	40.0	36.0	41.0	33.0	41.0
23	37.74710596616207	40.0	36.0	41.0	33.0	41.0
24	37.68631704590667	39.0	35.0	41.0	33.0	41.0
25	37.58300335347947	39.0	35.0	41.0	33.0	41.0
26	37.453231276406285	39.0	35.0	41.0	33.0	41.0
27	37.34966181012106	39.0	35.0	41.0	33.0	41.0
28	37.33579318315303	39.0	35.0	41.0	33.0	41.0
29	37.34753983441011	39.0	35.0	41.0	33.0	41.0
30	37.2819101570641	39.0	35.0	41.0	33.0	41.0
31	37.15387165836191	39.0	35.0	41.0	32.0	41.0
32	37.01768628862659	39.0	35.0	41.0	32.0	41.0
33	36.93587654648453	39.0	35.0	41.0	31.0	41.0
34	36.81085049544344	39.0	35.0	41.0	31.0	41.0
35	36.71681097364582	39.0	35.0	41.0	31.0	41.0
36	36.5828991493151	39.0	35.0	41.0	31.0	41.0
37	36.47017866277638	39.0	35.0	41.0	30.0	41.0
38	36.37415926185559	39.0	35.0	40.0	30.0	41.0
39	36.350343873742446	39.0	35.0	40.0	30.0	41.0
40	36.14571531422292	39.0	35.0	40.0	30.0	41.0
41	36.101968511396144	38.0	35.0	40.0	30.0	41.0
42	36.045622477785564	38.0	35.0	40.0	30.0	41.0
43	36.03568518974631	38.0	35.0	40.0	30.0	41.0
44	35.82105303044656	38.0	35.0	40.0	29.0	41.0
45	35.71413955779542	38.0	35.0	40.0	29.0	41.0
46	35.59232488963832	38.0	35.0	40.0	28.0	41.0
47	35.46714726890358	38.0	34.0	40.0	28.0	41.0
48	35.367082851783785	38.0	34.0	40.0	27.0	41.0
49	35.278471419639644	38.0	34.0	40.0	27.0	41.0
50	35.00308823250791	37.0	34.0	40.0	26.0	41.0
51	32.82130880430458	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	9.0
10	5.0
11	5.0
12	8.0
13	4.0
14	9.0
15	9.0
16	13.0
17	22.0
18	39.0
19	79.0
20	107.0
21	183.0
22	267.0
23	388.0
24	521.0
25	746.0
26	965.0
27	1129.0
28	1360.0
29	1596.0
30	2060.0
31	2600.0
32	3471.0
33	5190.0
34	8575.0
35	10464.0
36	9650.0
37	15546.0
38	19661.0
39	20865.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42034065288645	18.464977927663366	22.894602224285254	14.220079195164928
2	29.128853185805497	25.68537920842727	28.06312877740096	17.12263882836627
3	32.70400333453326	24.12042212159679	27.656732536329358	15.518842007540593
4	28.49036585134802	25.63801367916485	27.206760008336335	18.66486046115079
5	25.47791819025786	30.61707811523086	24.975843580076162	18.929160114435117
6	27.609367007066936	34.43473977378223	23.558666944544438	14.397226274606393
7	60.83628578465736	28.370057407021466	6.446448532615904	4.347208275705273
8	82.53348742918854	5.693336617343362	7.590799719596067	4.182376233872037
9	76.58153502207233	7.760368314355545	9.644569068414771	6.013527595157348
10	44.85420890093026	24.788276084196966	15.945131770902407	14.412383243970368
11	36.18536973532142	24.078740455845853	22.642617608609157	17.093272200223566
12	32.55717019381974	23.1143782800629	26.150508705784283	18.177942820333072
13	24.44819158409276	28.18154260055702	28.5993065686516	18.770959246698624
14	20.022356529811862	30.92400674485137	28.539626001780942	20.514010723555824
15	19.15367272313901	26.103143176521854	36.72533676891306	18.017847331426083
16	22.28737613914098	23.783179553248328	33.82088251454122	20.108561793069477
17	21.737935999696862	23.474356302457323	28.82760841969648	25.960099278149336
18	24.03611147950967	23.752865614520378	30.62086735757185	21.590155548398098
19	24.873534036869327	26.634584414846252	26.818362668384456	21.673518879899962
20	27.274966370474225	25.13972831132415	27.29675451393494	20.288550804266688
21	24.59218279305053	26.57869309031659	27.783672154752658	21.04545196188022
22	22.736401356548757	25.238248612189995	27.46348117693867	24.561868854322576
23	21.916977700308824	27.11487088156723	28.131335139538848	22.836816278585097
24	23.770864515640096	23.68087001004149	30.717493037267197	21.830772437051213
25	20.9961918114473	25.91747030181315	28.882552433640896	24.203785453098654
26	20.05645971088081	28.67035486254523	28.992440461529718	22.28074496504424
27	21.61857486595555	28.00155358935981	28.399424035164166	21.98044750952047
28	19.298611242682025	26.574903847975595	31.755745438699535	22.370739470642846
29	20.60874178208067	24.407457228927075	31.961311835698453	23.022489153293797
30	22.612303669881207	26.27365908186658	29.884807032833784	21.229230215418426
31	24.673651503381897	25.45991928913814	27.34696197495311	22.519467232526857
32	24.769329872491998	25.73937591178644	28.39847672457892	21.092817491142647
33	25.43623652450692	25.983782042780547	27.135711714442696	21.444269718269833
34	21.526685739186448	25.66169644379606	29.864913510543566	22.94670430647392
35	21.91981963206457	24.988158617684395	30.471192285102592	22.620829465148443
36	24.715333169132833	24.606392451829258	28.214698471040716	22.463575907997196
37	21.99560447888445	27.532634849661807	29.639453591254426	20.832307080199314
38	22.00507758473693	27.341278111441618	27.42748337469923	23.226160929122223
39	22.601883253443475	25.287508762622913	29.287054053541993	22.82355393039162
40	23.892120270551903	23.964115875030785	29.391258217919326	22.752505636497983
41	19.77131922472102	24.87258672628408	29.312631439343704	26.0434626096512
42	21.560788920255394	24.74943635020178	28.491313161933274	25.19846156760956
43	23.682764631211988	23.57761315624941	27.99397510467782	24.745647107860783
44	21.805195051249502	25.951573482882097	28.574676493435135	23.668554972433263
45	21.44048047592884	26.308709573520773	27.860404312157783	24.390405638392604
46	22.9599666546674	26.214925825581176	28.67982796839772	22.14527955135371
47	21.224493662492186	25.762111365832403	30.168052897823078	22.845342073852333
48	22.490100604384153	23.178795399859798	30.127318542657395	24.203785453098654
49	21.685833917508194	22.835868967999847	31.26503855554082	24.213258558951136
50	21.379852598472933	25.28182489911142	29.690608362857844	23.647714139557795
51	21.19702165551998	24.92847805081374	27.170762206096892	26.70373808756939
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	136.0
2	243.0
3	326.0
4	409.0
5	317.0
6	225.0
7	199.5
8	174.0
9	187.5
10	201.0
11	215.0
12	229.0
13	222.0
14	215.0
15	244.0
16	273.0
17	265.0
18	257.0
19	263.0
20	269.0
21	342.0
22	415.0
23	428.0
24	441.0
25	565.0
26	811.5
27	934.0
28	1120.5
29	1307.0
30	1585.5
31	1864.0
32	2122.5
33	2381.0
34	2630.0
35	2879.0
36	3024.5
37	3170.0
38	3604.5
39	4039.0
40	4785.0
41	5531.0
42	6383.5
43	7236.0
44	7691.0
45	8146.0
46	8737.0
47	9328.0
48	9388.5
49	9449.0
50	9298.5
51	9148.0
52	8030.5
53	6913.0
54	6203.5
55	5494.0
56	5214.5
57	4935.0
58	4651.0
59	4367.0
60	4211.5
61	4056.0
62	3701.0
63	3346.0
64	2873.5
65	2401.0
66	2041.0
67	1681.0
68	1384.5
69	1088.0
70	927.5
71	767.0
72	604.0
73	441.0
74	356.0
75	201.5
76	132.0
77	108.5
78	85.0
79	62.5
80	40.0
81	33.0
82	26.0
83	18.0
84	10.0
85	11.5
86	13.0
87	8.5
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	3.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	105562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.78388056308141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.39995140323168	32.00015156969364
2	10.731786336208641	10.041492203633883
3	5.750617583930668	8.071086186317046
4	3.881666869153201	7.263977567685341
5	2.6444741424695257	6.185938121672572
6	2.1220588830842746	5.95668896004244
7	1.6441906613210222	5.3845133665523575
8	1.2250435346049489	4.584983232602641
9	0.9982586158020492	4.203217066747504
>10	2.5776535860365284	15.066974858377066
>50	0.008099461385817843	0.20177715465792617
>100	0.016198922771635687	1.039199712017582
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	190	0.1799890111972111	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	170	0.16104279949224154	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144	0.13641272427578105	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	140	0.13262348193478712	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	128	0.12125575491180539	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	123	0.116519201985563	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13546541369053258	0.0
2	0.0	0.0	0.0	0.6555389249919479	0.0
3	0.0	0.0	0.0	0.8279494515071711	0.0
4	0.0	0.0	0.0	1.0571986131373032	0.0
5	0.0	0.0	0.0	1.8084159072393475	0.0
6	0.0	0.0	0.0	2.247968018794642	0.0
7	0.0	0.0	0.0	2.6789943350827	0.0
8	0.0	0.0	0.0	3.4917868172258957	0.0
9	0.0	0.0	0.0	3.773138061044694	0.0
10	0.0	0.0	0.0	4.7138174721964345	0.0
11	0.0	0.0	0.0	5.627024876375969	0.0
12	0.0	0.0	0.0	6.530759174703018	0.0
13	0.0	0.0	0.0	6.8509501525170045	0.0
14	0.0	0.0	0.0	6.9693639756730645	0.0
15	0.0	0.0	0.0	7.228927076031147	0.0
16	0.0	0.0	0.0	7.730054375627593	0.0
17	0.0	0.0	0.0	8.328754665504633	0.0
18	0.0	0.0	0.0	9.029764498588507	0.0
19	0.0	0.0	0.0	9.422898391466626	0.0
20	0.0	0.0	0.0	9.795191451469279	0.0
21	0.0	0.0	0.0	10.258426327655785	0.0
22	0.0	0.0	0.0	10.798393361247419	0.0
23	0.0	0.0	0.0	11.28341638089464	0.0
24	0.0	0.0	0.0	11.701180348989219	0.0
25	0.0	0.0	0.0	12.052632576116405	0.0
26	0.0	0.0	0.0	12.41734715143707	0.0
27	0.0	0.0	0.0	12.747958545688789	0.0
28	0.0	0.0	0.0	13.12214622686194	0.0
29	0.0	0.0	0.0	13.47738769633012	0.0
30	0.0	0.0	0.0	13.896098975009947	0.0
31	0.0	0.0	0.0	14.271233966768344	0.0
32	0.0	0.0	0.0	14.619844262139786	0.0
33	0.0	0.0	0.0	14.991190011557189	0.0
34	0.0	0.0	0.0	15.355904586877854	0.0
35	0.0	0.0	0.0	15.755669653852712	0.0
36	0.0	0.0	0.0	16.127015403270118	0.0
37	0.0	0.0	0.0	16.49362459976128	0.0
38	0.0	0.0	0.0	16.8223413728425	0.0
39	0.0	0.0	0.0	17.181372084651674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCCCG	20	7.004176E-4	45.0	14
AGCCCGA	20	7.004176E-4	45.0	15
TATGCCA	20	7.004176E-4	45.0	10
TGTGCGG	25	3.8666964E-5	45.0	2
ACGGGCT	20	7.004176E-4	45.0	5
TCGGAGC	20	7.004176E-4	45.0	36
GATTGGG	35	1.1979682E-7	45.0	3
CGAGATA	25	3.8666964E-5	45.0	19
AAGCGGG	55	6.002665E-11	40.909092	3
GGCGATA	40	3.4185905E-7	39.375	8
TGGGAAC	40	3.4185905E-7	39.375	6
GGACAGC	40	3.4185905E-7	39.375	8
TGACGGG	35	6.194541E-6	38.571426	3
AAGGCGA	35	6.194541E-6	38.571426	6
TGATGGG	95	0.0	37.894737	3
ATTTAGC	60	1.5279511E-10	37.499996	20
ATGGGAT	60	1.5279511E-10	37.499996	5
ATAGGGT	30	1.1329129E-4	37.499996	5
ATAGGAG	30	1.1329129E-4	37.499996	5
CACGGGA	30	1.1329129E-4	37.499996	4
>>END_MODULE