##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152404
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09348835988557	33.0	31.0	34.0	30.0	34.0
2	32.21575549198184	33.0	31.0	34.0	30.0	34.0
3	32.11636833678906	33.0	31.0	34.0	30.0	34.0
4	35.83808167764626	37.0	35.0	37.0	35.0	37.0
5	35.89667594026403	37.0	35.0	37.0	35.0	37.0
6	35.85964935303535	37.0	35.0	37.0	35.0	37.0
7	26.708052282092332	36.0	10.0	37.0	0.0	37.0
8	31.108468281672398	36.0	19.0	37.0	17.0	37.0
9	35.988983228786644	39.0	32.0	39.0	32.0	39.0
10	36.71519776383822	38.0	35.0	39.0	33.0	39.0
11	37.06253772866854	39.0	37.0	39.0	34.0	39.0
12	37.06650744074959	39.0	37.0	39.0	34.0	39.0
13	36.94360384241883	39.0	37.0	39.0	33.0	39.0
14	38.10253011731975	40.0	37.0	41.0	33.0	41.0
15	38.19908270124144	40.0	37.0	41.0	33.0	41.0
16	38.19004750531482	40.0	37.0	41.0	33.0	41.0
17	38.1225886459673	40.0	37.0	41.0	33.0	41.0
18	38.02904779402115	40.0	37.0	41.0	33.0	41.0
19	37.91294191753497	40.0	36.0	41.0	33.0	41.0
20	37.96889845410882	40.0	36.0	41.0	33.0	41.0
21	37.906347602425136	40.0	36.0	41.0	33.0	41.0
22	37.91420828849636	40.0	36.0	41.0	33.0	41.0
23	37.87874334006982	40.0	36.0	41.0	33.0	41.0
24	37.82321330148815	40.0	36.0	41.0	33.0	41.0
25	37.728675100391065	40.0	36.0	41.0	33.0	41.0
26	37.60723471824886	40.0	36.0	41.0	33.0	41.0
27	37.51763077084591	40.0	36.0	41.0	33.0	41.0
28	37.47069630718354	39.0	35.0	41.0	33.0	41.0
29	37.435014829007116	40.0	36.0	41.0	33.0	41.0
30	37.356624498044674	39.0	35.0	41.0	33.0	41.0
31	37.21815044224561	39.0	35.0	41.0	32.0	41.0
32	37.08049657489305	39.0	35.0	41.0	32.0	41.0
33	36.999986876984856	39.0	35.0	41.0	31.0	41.0
34	36.82571979738064	39.0	35.0	41.0	31.0	41.0
35	36.73390462192593	39.0	35.0	41.0	31.0	41.0
36	36.581782630377155	39.0	35.0	41.0	30.0	41.0
37	36.52543896485656	39.0	35.0	41.0	30.0	41.0
38	36.34804204614052	39.0	35.0	41.0	30.0	41.0
39	36.290589485840265	39.0	35.0	41.0	30.0	41.0
40	36.0824519041495	39.0	35.0	40.0	29.0	41.0
41	36.00580037269363	39.0	35.0	40.0	29.0	41.0
42	35.93086139471405	39.0	35.0	40.0	29.0	41.0
43	35.933886249704734	38.0	35.0	40.0	29.0	41.0
44	35.759074564972046	38.0	35.0	40.0	28.0	41.0
45	35.65529776121362	38.0	35.0	40.0	28.0	41.0
46	35.57922364242408	38.0	34.0	40.0	28.0	41.0
47	35.4361893388625	38.0	34.0	40.0	27.0	41.0
48	35.32778011075825	38.0	34.0	40.0	27.0	41.0
49	35.18426025563633	38.0	34.0	40.0	26.0	41.0
50	35.00556415842104	38.0	34.0	40.0	26.0	41.0
51	32.937239180074016	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	9.0
10	15.0
11	11.0
12	6.0
13	8.0
14	7.0
15	16.0
16	15.0
17	27.0
18	51.0
19	117.0
20	167.0
21	295.0
22	466.0
23	624.0
24	808.0
25	1189.0
26	1462.0
27	1804.0
28	1991.0
29	2337.0
30	2881.0
31	3717.0
32	4926.0
33	6917.0
34	11155.0
35	13427.0
36	14430.0
37	23022.0
38	28926.0
39	31565.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93588094800661	18.67601900212593	24.2552688905803	14.132831159287157
2	29.465762053489406	25.089236502978924	27.433663158447285	18.01133828508438
3	31.877116086191965	24.5151045904307	27.175795910868484	16.431983412508856
4	28.098343875488833	26.173853704627177	26.687619747513185	19.040182672370804
5	26.104301724364188	30.493950290018635	24.572189706306922	18.829558279310255
6	26.973045326894308	35.16508753051101	23.861578436261517	14.000288706333167
7	61.63945828193486	28.578646229757748	5.986063357917115	3.795832130390279
8	84.2970000787381	5.492637988504239	6.917141282381039	3.2932206503766306
9	78.62063987821843	7.330516259415763	8.627726306396157	5.421117555969659
10	44.48898979029422	25.686333692029077	15.838166977244692	13.98650954043201
11	33.9361171622792	25.64105928978242	23.45804572058476	16.964777827353615
12	29.441484475473086	23.470512584971523	27.501246686438673	19.586756253116715
13	24.979659326526864	26.656780661924884	29.55499855646833	18.808561455079918
14	21.588016062570535	27.734836356001153	29.965092779717068	20.71205480171124
15	20.98698196897719	25.12598094538201	33.774704076008504	20.112333009632295
16	24.377312931419123	24.822839295556548	30.246581454555	20.553266318469333
17	25.432403348993464	24.404871263221438	27.396262565287	22.766462822498095
18	26.307708459095565	24.574158158578516	28.682974200152227	20.435159182173695
19	26.429752499934384	26.978294532951892	25.8096900343823	20.782262932731424
20	26.919240964804075	26.815569145166794	27.06949948820241	19.195690401826724
21	25.786068607123173	27.329335188052806	27.741397863573138	19.143198341250887
22	25.241463478648857	24.362877614760766	28.190861132253747	22.204797774336633
23	23.51972389176137	26.763077084590957	28.07275399595811	21.644445027689564
24	23.60502349019711	25.56100889740427	29.511692606493266	21.322275005905354
25	22.643762630902074	27.842445080181623	26.94942389963518	22.56436838928112
26	21.708091651137764	28.31356132384977	27.032755045799323	22.945591979213145
27	22.55124537413716	27.28996614262093	28.615390672160835	21.543397811081075
28	21.248129970341985	27.181045116926068	29.79843048738878	21.772394425343165
29	22.307813443216716	26.57804257106113	28.681005747880633	22.433138237841526
30	24.30972940342773	25.4527440224666	28.73349780845647	21.504028765649196
31	25.219154352904123	26.08068029710506	26.645626099052517	22.054539250938294
32	25.240807327891655	27.09574551849033	26.383821946930524	21.279625206687488
33	24.690296842602557	26.183695965985144	26.501929083226162	22.624078108186136
34	22.080785281226216	26.863468150442245	29.254481509671663	21.801265058659876
35	23.288102674470487	27.95267840739088	27.737460959029946	21.021757959108687
36	24.636492480512324	27.443505419805252	26.67121597858324	21.248786121099183
37	23.47116873572872	28.734810109970866	26.646938400566917	21.1470827537335
38	22.389176137109263	28.09506312170284	26.289992388651218	23.22576835253668
39	23.30385029264324	26.399569565103278	27.515025852339836	22.78155428991365
40	24.486890107871183	26.398913414346083	26.85100128605548	22.26319519172725
41	21.582110705755756	25.877929713130886	27.963832970263248	24.57612661085011
42	22.87603999895016	25.9724154221674	28.247290097372773	22.90425448150967
43	23.27301120705493	25.515734495157606	28.107529986089602	23.103724311697857
44	22.553213826408754	27.18498202146925	26.86543660271384	23.39636754940815
45	21.88853310936721	27.051783417758063	26.488806068082205	24.570877404792522
46	22.906879084538463	27.270281619904992	26.177790609170366	23.645048686386183
47	21.215978583239284	26.629878480879764	29.468386656518202	22.685756279362746
48	22.624734258943334	26.498648329440172	28.418545445001442	22.45807196661505
49	22.706096952835882	25.7768824965224	28.33849505262329	23.178525498018423
50	21.271751397601115	27.057688774572846	28.05700637778536	23.61355345004068
51	21.675940264035066	26.87527886407181	26.38250964541613	25.066271226476992
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	139.0
2	234.0
3	482.5
4	731.0
5	542.5
6	354.0
7	312.5
8	271.0
9	268.0
10	265.0
11	250.5
12	236.0
13	255.5
14	275.0
15	292.5
16	310.0
17	312.5
18	315.0
19	338.5
20	362.0
21	391.5
22	421.0
23	538.5
24	656.0
25	818.5
26	1262.5
27	1544.0
28	1823.5
29	2103.0
30	2493.0
31	2883.0
32	3261.0
33	3639.0
34	4144.0
35	4649.0
36	5128.5
37	5608.0
38	5962.0
39	6316.0
40	7116.0
41	7916.0
42	8599.0
43	9282.0
44	10030.0
45	10778.0
46	11588.5
47	12399.0
48	12000.5
49	11602.0
50	11639.0
51	11676.0
52	10906.0
53	10136.0
54	9581.0
55	9026.0
56	8525.5
57	8025.0
58	7555.5
59	7086.0
60	6528.0
61	5970.0
62	5555.0
63	5140.0
64	4400.5
65	3661.0
66	3098.5
67	2536.0
68	2188.0
69	1840.0
70	1560.5
71	1281.0
72	1017.0
73	753.0
74	624.0
75	402.5
76	310.0
77	217.0
78	124.0
79	104.5
80	85.0
81	62.0
82	39.0
83	33.0
84	27.0
85	17.5
86	8.0
87	5.5
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.66604551061652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.95426660734269	33.07065431353508
2	11.440089525273363	11.134878349649616
3	6.184524531812483	9.029290569801319
4	4.121668082352465	8.023411459016824
5	2.827326780730494	6.879740689220755
6	2.0669012660275854	6.0352746647069635
7	1.5248958459733852	5.194745544736358
8	1.14737963300031	4.467074355003805
9	0.7968288638110261	3.490065877536023
>10	1.9212878695951139	11.617149156190127
>50	0.006741360945947768	0.1955329256449962
>100	0.00808963313513732	0.8621820949581376
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.20668748851736174	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG	281	0.18437836277263064	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	224	0.14697776961234615	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC	220	0.14435316658355424	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	156	0.10235951812288392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.561507571979738E-4	0.0	0.0	0.034775990131492615	0.0
2	6.561507571979738E-4	0.0	0.0	0.24080732789165638	0.0
3	6.561507571979738E-4	0.0	0.0	0.31954541875541326	0.0
4	6.561507571979738E-4	0.0	0.0	0.4534001732237999	0.0
5	6.561507571979738E-4	0.0	0.0	0.9389517335503005	0.0
6	6.561507571979738E-4	0.0	0.0	1.231594971260597	0.0
7	6.561507571979738E-4	0.0	0.0	1.4979921786829742	0.0
8	6.561507571979738E-4	0.0	0.0	2.0137266738405817	0.0
9	6.561507571979738E-4	0.0	0.0	2.207947297971182	0.0
10	6.561507571979738E-4	0.0	0.0	2.8896879346998765	0.0
11	6.561507571979738E-4	0.0	0.0	3.4546337366473323	0.0
12	6.561507571979738E-4	0.0	0.0	4.0746962021994175	0.0
13	6.561507571979738E-4	0.0	0.0	4.262355318758038	0.0
14	6.561507571979738E-4	0.0	0.0	4.364058686123724	0.0
15	6.561507571979738E-4	0.0	0.0	4.524815621637227	0.0
16	6.561507571979738E-4	0.0	0.0	4.80892889950395	0.0
17	6.561507571979738E-4	0.0	0.0	5.169155665205638	0.0
18	6.561507571979738E-4	0.0	0.0	5.595653657384321	0.0
19	6.561507571979738E-4	0.0	0.0	5.828587176189601	0.0
20	6.561507571979738E-4	0.0	0.0	6.062832996509278	0.0
21	6.561507571979738E-4	0.0	0.0	6.372536153906721	0.0
22	6.561507571979738E-4	0.0	0.0	6.695362326448125	0.0
23	6.561507571979738E-4	0.0	0.0	7.050339886092228	0.0
24	6.561507571979738E-4	0.0	0.0	7.325267053358179	0.0
25	6.561507571979738E-4	0.0	0.0	7.579197396393796	0.0
26	6.561507571979738E-4	0.0	0.0	7.823285478071441	0.0
27	6.561507571979738E-4	0.0	0.0	8.097556494580195	0.0
28	6.561507571979738E-4	0.0	0.0	8.346237631558227	0.0
29	6.561507571979738E-4	0.0	0.0	8.616571743523792	0.0
30	6.561507571979738E-4	0.0	0.0	8.91183958426288	0.0
31	6.561507571979738E-4	0.0	0.0	9.21629353560274	0.0
32	6.561507571979738E-4	0.0	0.0	9.548305818744915	0.0
33	6.561507571979738E-4	0.0	0.0	9.816015327681688	0.0
34	6.561507571979738E-4	0.0	0.0	10.10078475630561	0.0
35	6.561507571979738E-4	0.0	0.0	10.408519461431458	0.0
36	6.561507571979738E-4	0.0	0.0	10.693945040812578	0.0
37	6.561507571979738E-4	0.0	0.0	11.019395816382772	0.0
38	6.561507571979738E-4	0.0	0.0	11.336972782866592	0.0
39	6.561507571979738E-4	0.0	0.0	11.825805096979082	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	40	6.7484507E-9	45.0	12
ACGTGAG	25	3.8748454E-5	45.0	44
AAGGGCG	40	6.7484507E-9	45.0	5
TGGGCAT	25	3.8748454E-5	45.0	6
CACGTGA	25	3.8748454E-5	45.0	43
AATAGGC	20	7.014022E-4	45.0	4
TCAAGCG	25	3.8748454E-5	45.0	17
GACTAAT	25	3.8748454E-5	45.0	9
AACACGT	25	3.8748454E-5	45.0	41
CTACGAA	45	1.909575E-8	40.0	11
CGAATAT	45	1.909575E-8	40.0	14
TGACTAT	45	1.909575E-8	40.0	23
GGTATGC	40	3.4319783E-7	39.375	8
GAACATA	40	3.4319783E-7	39.375	9
CACTAGG	35	6.2126946E-6	38.571426	2
CGGTAGT	35	6.2126946E-6	38.571426	12
ACACGTG	30	1.1352816E-4	37.500004	42
CACGACC	30	1.1352816E-4	37.500004	27
TCTAAGG	30	1.1352816E-4	37.500004	2
AAACACG	30	1.1352816E-4	37.500004	40
>>END_MODULE