##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60066
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837145806279757	31.0	31.0	34.0	30.0	34.0
2	31.958329171244962	33.0	31.0	34.0	30.0	34.0
3	31.850997236373324	33.0	31.0	34.0	30.0	34.0
4	35.63137215729364	37.0	35.0	37.0	33.0	37.0
5	35.71349848499983	37.0	35.0	37.0	35.0	37.0
6	35.680001997802414	37.0	35.0	37.0	35.0	37.0
7	26.850930642959412	35.0	11.0	37.0	0.0	37.0
8	31.156178203975628	35.0	19.0	37.0	17.0	37.0
9	35.8721239969367	39.0	32.0	39.0	32.0	39.0
10	36.62075050777478	37.0	35.0	39.0	32.0	39.0
11	36.88530949289115	39.0	37.0	39.0	33.0	39.0
12	36.70264375853228	39.0	35.0	39.0	33.0	39.0
13	36.36131921552958	39.0	35.0	39.0	32.0	39.0
14	37.38411081144075	39.0	35.0	41.0	33.0	41.0
15	37.5414710485133	39.0	35.0	41.0	33.0	41.0
16	37.58454033896048	39.0	35.0	41.0	33.0	41.0
17	37.51042186927713	39.0	35.0	41.0	33.0	41.0
18	37.43948323510805	39.0	35.0	41.0	33.0	41.0
19	37.34024240002664	39.0	35.0	41.0	32.0	41.0
20	37.411730429860484	39.0	35.0	41.0	33.0	41.0
21	37.34253987280658	39.0	35.0	41.0	33.0	41.0
22	37.381280591349515	39.0	35.0	41.0	33.0	41.0
23	37.3653647654247	39.0	35.0	41.0	33.0	41.0
24	37.314737122498585	39.0	35.0	41.0	33.0	41.0
25	37.22651749741951	39.0	35.0	41.0	33.0	41.0
26	37.07626610728199	39.0	35.0	41.0	32.0	41.0
27	36.967702194252986	39.0	35.0	40.0	32.0	41.0
28	36.98090433856092	39.0	35.0	40.0	32.0	41.0
29	37.00361269270469	39.0	35.0	41.0	32.0	41.0
30	36.91361169380348	39.0	35.0	40.0	32.0	41.0
31	36.76282755635468	38.0	35.0	40.0	31.0	41.0
32	36.616854793060966	38.0	35.0	40.0	31.0	41.0
33	36.478823294376184	38.0	35.0	40.0	31.0	41.0
34	36.359138281223984	38.0	35.0	40.0	30.0	41.0
35	36.26029700662605	38.0	35.0	40.0	30.0	41.0
36	36.019478573569074	38.0	35.0	40.0	30.0	41.0
37	35.83969300436187	38.0	35.0	40.0	30.0	41.0
38	35.72758299204209	38.0	35.0	40.0	29.0	41.0
39	35.711384144108145	38.0	35.0	40.0	29.0	41.0
40	35.4489894449439	38.0	34.0	40.0	27.0	41.0
41	35.46860120534079	38.0	34.0	40.0	28.0	41.0
42	35.3486831152399	38.0	34.0	40.0	27.0	41.0
43	35.22555189291779	38.0	34.0	40.0	27.0	41.0
44	34.99723637332268	37.0	34.0	40.0	26.0	41.0
45	34.91359504545	37.0	34.0	40.0	26.0	41.0
46	34.79191222988047	37.0	34.0	40.0	26.0	41.0
47	34.640861718776016	37.0	33.0	40.0	24.0	41.0
48	34.55500615989079	37.0	34.0	40.0	24.0	41.0
49	34.38712416342024	36.0	33.0	40.0	24.0	41.0
50	34.12239869476909	36.0	33.0	40.0	23.0	41.0
51	31.854693170845405	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	3.0
12	1.0
13	1.0
14	0.0
15	2.0
16	6.0
17	10.0
18	22.0
19	34.0
20	67.0
21	126.0
22	171.0
23	234.0
24	373.0
25	531.0
26	702.0
27	846.0
28	1053.0
29	1221.0
30	1460.0
31	1785.0
32	2390.0
33	3395.0
34	5452.0
35	6874.0
36	5469.0
37	7932.0
38	9933.0
39	9959.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.654147104851326	19.781573602370724	24.631238970465823	16.933040322312124
2	25.65344787400526	27.940931641860622	28.33383278393767	18.071787700196452
3	30.08523956980655	25.740019312090034	28.67012952419006	15.504611593913362
4	27.985882196250788	24.949222521892587	29.27945926147904	17.785436020377585
5	25.585189624746114	29.054706489528186	26.04634901608231	19.313754869643393
6	24.949222521892587	35.89884460426864	24.99250824093497	14.159424632903805
7	59.76425931475377	27.752805247561017	8.297539373355974	4.185396064329238
8	80.05693736889422	5.765324809376353	9.003429560816436	5.174308260912996
9	75.03745879532514	6.679319415309825	10.803116571771051	7.48010521759398
10	46.059334731795026	24.56964006259781	16.478540272367063	12.892484933240103
11	37.41217993540439	21.870941963839776	24.591282922119003	16.125595178636836
12	33.69626743915027	20.682249525521925	27.57466786534812	18.046815169979688
13	24.00193120900343	29.875470315985748	30.218426397629273	15.904172077381547
14	17.867012952419007	33.29337728498651	29.078014184397162	19.761595578197316
15	16.12060067259348	24.30160157160457	43.212466287084204	16.365331468717745
16	19.595112043418904	22.771617886990978	37.20907002297473	20.42420004661539
17	19.851496686977658	22.45196950021643	29.897113175506938	27.799420637298972
18	23.054639896114274	23.37761795358439	33.12356407951253	20.444178070788798
19	24.531348849598775	26.3093930010322	27.421503013351977	21.737755136017046
20	24.834348882895483	27.23504145440016	27.76778876569107	20.162820897013287
21	22.33043651982819	28.064129457596643	29.342723004694836	20.26271101788033
22	20.545733027003628	24.664535677421505	28.022508573902037	26.76722272167283
23	17.285985416042355	29.395997735823926	28.87157460127193	24.446442246861785
24	21.794359537841707	23.274398162021775	32.59414643891719	22.337095861219325
25	18.567908633836115	27.268338161355842	30.118536276762224	24.045216928045814
26	18.076782206239802	29.707321945859555	28.67012952419006	23.545766323710584
27	19.854826357673225	30.33829454266973	28.889887790097557	20.916991309559485
28	16.11227649585456	28.57023940332301	34.21736090300669	21.100123197815737
29	20.01798022175607	25.898178670129525	31.983151866280423	22.100689241833983
30	21.028535277861018	28.770019645057104	28.898211966836477	21.303233110245394
31	24.206705956780876	27.83937668564579	25.29883794492725	22.65507941264609
32	25.00083241767389	29.95704724802717	24.03023340991576	21.011886924383177
33	22.590150834082507	29.650717544034894	23.638997103186497	24.1201345186961
34	19.701661505677087	28.000865714380847	27.354909599440614	24.94256318050145
35	18.54959544501049	28.710085572536876	26.981986481536975	25.75833250091566
36	24.42313455199281	28.015849232510902	26.620717211067824	20.94029900442846
37	18.829287783438218	31.282256184863318	28.735058102753637	21.153397928944827
38	17.765457996204177	31.663503479505877	24.441447740818433	26.129590783471514
39	19.956381313888055	29.16292078713415	26.697299637065896	24.183398261911897
40	21.899244164752105	24.45310158825292	26.88709086671328	26.76056338028169
41	17.076216162221556	26.269436952685382	25.6384643558752	31.01588252921786
42	21.136749575466986	25.062431325541905	27.016948023840442	26.783871075150667
43	21.817667232710686	27.04025571870942	27.036926048013854	24.105151000566043
44	19.08900209769254	28.38044817367562	28.41873938667466	24.11181034195718
45	18.55958445709719	28.854926247794094	25.37375553557753	27.21173375953118
46	22.6117936936037	28.10075583524789	26.26444244664203	23.023008024506375
47	17.968567908633837	27.121832650750843	29.60576698964472	25.3038324509706
48	21.005227582992042	24.89095328472014	28.472014117803752	25.631805014484065
49	20.50577697865681	22.325442013784837	31.79502547198082	25.37375553557753
50	20.116205507275332	24.9059368028502	29.718975793294046	25.258881896580426
51	19.030732860520093	26.184530349948393	25.370425864881962	29.414310924649552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	76.0
2	129.0
3	257.5
4	386.0
5	278.5
6	171.0
7	146.5
8	122.0
9	132.5
10	143.0
11	149.5
12	156.0
13	141.0
14	126.0
15	116.0
16	106.0
17	124.5
18	143.0
19	136.5
20	130.0
21	132.5
22	135.0
23	165.0
24	195.0
25	278.5
26	439.0
27	516.0
28	566.0
29	616.0
30	725.5
31	835.0
32	981.5
33	1128.0
34	1316.5
35	1505.0
36	1664.0
37	1823.0
38	2027.5
39	2232.0
40	2759.5
41	3287.0
42	3891.5
43	4496.0
44	5250.5
45	6005.0
46	6761.5
47	7518.0
48	6845.0
49	6172.0
50	5881.5
51	5591.0
52	4777.5
53	3964.0
54	3511.5
55	3059.0
56	2732.0
57	2405.0
58	2182.0
59	1959.0
60	1764.5
61	1570.0
62	1323.5
63	1077.0
64	875.5
65	674.0
66	602.0
67	530.0
68	424.0
69	318.0
70	248.5
71	179.0
72	138.5
73	98.0
74	80.5
75	55.0
76	47.0
77	38.0
78	29.0
79	21.0
80	13.0
81	15.5
82	18.0
83	12.0
84	6.0
85	4.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	60066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.77694536010389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0134380618908	39.062031765058435
2	10.097473265827576	10.658275896513834
3	4.854736443645311	7.686544800719208
4	2.9809785180278223	6.293077614623914
5	1.97154663890729	5.2026104618253255
6	1.520456767925302	4.814703825791629
7	1.1860824579666256	4.381846635367762
8	0.9211065896974858	3.8890553724236674
9	0.6435128229393395	3.056637698531615
>10	1.7696602630831832	11.632204574967536
>50	0.012617898489006657	0.4694835680751174
>100	0.028390271600264975	2.8535277861019543
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	334	0.5560550061598908	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	320	0.5327473112909134	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	279	0.464489062031765	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.30133519794892283	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	162	0.26970332634102484	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	115	0.19145606499517198	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	111	0.18479672360403554	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	110	0.18313188825625146	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	102	0.16981320547397863	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT	85	0.14151100456164883	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	78	0.12985715712716012	No Hit
GAATCTATCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	64	0.10654946225818268	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2463956314720474	0.0
2	0.0	0.0	0.0	1.1470715546232477	0.0
3	0.0	0.0	0.0	1.4184397163120568	0.0
4	0.0	0.0	0.0	1.7863683281723437	0.0
5	0.0	0.0	0.0	3.2714014583957645	0.0
6	0.0	0.0	0.0	3.9373355975094064	0.0
7	0.0	0.0	0.0	4.7281323877068555	0.0
8	0.0	0.0	0.0	6.48619851496687	0.0
9	0.0	0.0	0.0	7.050577697865681	0.0
10	0.0	0.0	0.0	9.01341857290314	0.0
11	0.0	0.0	0.0	10.426863783171845	0.0
12	0.0	0.0	0.0	12.358072786601404	0.0
13	0.0	0.0	0.0	12.925781640195785	0.0
14	0.0	0.0	0.0	13.130556387973229	0.0
15	0.0	0.0	0.0	13.510138847268005	0.0
16	0.0	0.0	0.0	14.241001564945227	0.0
17	0.0	0.0	0.0	15.13834781740086	0.0
18	0.0	0.0	0.0	16.252122665068423	0.0
19	0.0	0.0	0.0	16.83481503679286	0.0
20	0.0	0.0	0.0	17.41084806712616	0.0
21	0.0	0.0	0.0	18.183331668497985	0.0
22	0.0	0.0	0.0	18.8942163620018	0.0
23	0.0	0.0	0.0	19.53517797089868	0.0
24	0.0	0.0	0.0	20.049612093363965	0.0
25	0.0	0.0	0.0	20.504112143309026	0.0
26	0.0	0.0	0.0	21.00023307694869	0.0
27	0.0	0.0	0.0	21.459727632937103	0.0
28	0.0	0.0	0.0	21.919222188925517	0.0
29	0.0	0.0	0.0	22.348749708653813	0.0
30	0.0	0.0	0.0	22.95142010455166	0.0
31	0.0	0.0	0.0	23.525788299537176	0.0
32	0.0	0.0	0.0	23.991942196916725	0.0
33	0.0	0.0	0.0	24.478074118469685	0.0
34	0.0	0.0	0.0	24.932574168414742	0.0
35	0.0	0.0	0.0	25.503612692704692	0.0
36	0.0	0.0	0.0	25.984750108214296	0.0
37	0.0	0.0	0.0	26.46755235907169	0.0
38	0.0	0.0	0.0	26.937035927146805	0.0
39	0.0	0.0	0.0	27.49142609795891	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTC	20	6.979949E-4	45.000004	5
TGTGAGG	20	6.979949E-4	45.000004	2
GTCACTT	20	6.979949E-4	45.000004	4
AGCTAGG	20	6.979949E-4	45.000004	3
GCTGTAC	20	6.979949E-4	45.000004	28
AAGGCGA	20	6.979949E-4	45.000004	6
GGGCGTC	20	6.979949E-4	45.000004	10
AACGGGA	20	6.979949E-4	45.000004	4
TTTGAAG	25	3.846665E-5	45.0	2
GGGTTCC	25	3.846665E-5	45.0	7
CTTTAGG	25	3.846665E-5	45.0	2
CGAGGGA	25	3.846665E-5	45.0	4
TATCTCT	30	2.1312026E-6	44.999996	6
TAAGGGA	60	3.6379788E-12	41.249996	4
ACGGCTG	50	1.0459189E-9	40.5	8
AAGGGAC	45	1.8764695E-8	40.0	5
AACTTAA	145	0.0	38.793102	45
AGGCGAT	35	6.1499686E-6	38.571426	7
TAGGGAG	35	6.1499686E-6	38.571426	5
CTATCTC	35	6.1499686E-6	38.571426	5
>>END_MODULE