##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108795
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01417344547084	33.0	31.0	34.0	30.0	34.0
2	32.12260673744198	33.0	31.0	34.0	30.0	34.0
3	32.02251941725263	33.0	31.0	34.0	30.0	34.0
4	35.76751688956294	37.0	35.0	37.0	35.0	37.0
5	35.85071005101337	37.0	35.0	37.0	35.0	37.0
6	35.832593409623605	37.0	35.0	37.0	35.0	37.0
7	26.823420193942738	35.0	10.0	37.0	0.0	37.0
8	31.193795670756927	36.0	19.0	37.0	17.0	37.0
9	35.99902569051886	39.0	32.0	39.0	32.0	39.0
10	36.745852291006024	38.0	35.0	39.0	33.0	39.0
11	37.05712578703066	39.0	37.0	39.0	34.0	39.0
12	37.00406268670435	39.0	36.0	39.0	34.0	39.0
13	36.828696171699065	39.0	35.0	39.0	33.0	39.0
14	37.97763683992831	40.0	37.0	41.0	33.0	41.0
15	38.16227767820212	40.0	37.0	41.0	33.0	41.0
16	38.13320465094903	40.0	37.0	41.0	34.0	41.0
17	38.05533342524932	40.0	36.0	41.0	34.0	41.0
18	37.940870444413804	40.0	36.0	41.0	33.0	41.0
19	37.81541431131945	40.0	36.0	41.0	33.0	41.0
20	37.887936026471806	40.0	36.0	41.0	34.0	41.0
21	37.79060618594605	40.0	36.0	41.0	33.0	41.0
22	37.79008226480996	40.0	36.0	41.0	33.0	41.0
23	37.7238016452962	40.0	36.0	41.0	33.0	41.0
24	37.69884645434073	39.0	35.0	41.0	33.0	41.0
25	37.56573371938049	39.0	35.0	41.0	33.0	41.0
26	37.41744565467163	39.0	35.0	41.0	33.0	41.0
27	37.308258651592446	39.0	35.0	41.0	33.0	41.0
28	37.30493129279838	39.0	35.0	41.0	33.0	41.0
29	37.30172342478974	39.0	35.0	41.0	33.0	41.0
30	37.22892596167103	39.0	35.0	41.0	32.0	41.0
31	37.100896180890665	39.0	35.0	41.0	32.0	41.0
32	36.93202812629257	39.0	35.0	41.0	31.0	41.0
33	36.84819155292063	39.0	35.0	41.0	31.0	41.0
34	36.69639229744014	39.0	35.0	41.0	31.0	41.0
35	36.63179374052116	39.0	35.0	41.0	31.0	41.0
36	36.45382600303323	39.0	35.0	41.0	30.0	41.0
37	36.37237924536973	39.0	35.0	41.0	30.0	41.0
38	36.29510547359713	39.0	35.0	40.0	30.0	41.0
39	36.24419320740843	39.0	35.0	40.0	30.0	41.0
40	36.03694103589319	38.0	35.0	40.0	29.0	41.0
41	35.98629532607197	38.0	35.0	40.0	29.0	41.0
42	35.93416976883129	38.0	35.0	40.0	29.0	41.0
43	35.920474286502134	38.0	35.0	40.0	29.0	41.0
44	35.726071970219216	38.0	35.0	40.0	29.0	41.0
45	35.6055884921182	38.0	35.0	40.0	28.0	41.0
46	35.50425111448136	38.0	34.0	40.0	28.0	41.0
47	35.387545383519466	38.0	34.0	40.0	27.0	41.0
48	35.32850774392205	38.0	34.0	40.0	27.0	41.0
49	35.182885242888	38.0	34.0	40.0	27.0	41.0
50	34.943545199687485	37.0	34.0	40.0	26.0	41.0
51	32.78076198354704	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	16.0
10	9.0
11	9.0
12	7.0
13	4.0
14	3.0
15	6.0
16	10.0
17	17.0
18	46.0
19	69.0
20	129.0
21	194.0
22	286.0
23	446.0
24	593.0
25	848.0
26	1104.0
27	1261.0
28	1476.0
29	1749.0
30	2178.0
31	2735.0
32	3544.0
33	5018.0
34	8486.0
35	10824.0
36	9823.0
37	15842.0
38	20319.0
39	21740.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53315869295464	19.395192793786478	24.004779631416884	15.066868881841996
2	30.04825589411278	24.933131118158002	26.91116319683809	18.107449790891124
3	32.7239303276805	23.802564456087136	27.842272163242797	15.631233052989568
4	29.585918470517946	25.27230111677926	26.607840433843467	18.533939978859323
5	26.050829541798798	30.625488303690428	24.447814697366606	18.87586745714417
6	26.922193115492437	35.93639413575991	23.263017601911855	13.878395146835793
7	61.154464819155294	28.744887173123768	6.149179649800083	3.9514683579208607
8	83.33103543361368	5.316420791396664	7.271473872880188	4.081069902109472
9	76.93827841353004	7.845948802794246	9.648421342892597	5.567351440783124
10	44.549841444919345	25.73188106071051	15.958453973068615	13.75982352130153
11	35.126614274553056	25.874350843329196	22.569051886575668	16.429982995542076
12	32.79654395882164	23.521301530401214	26.038880463256586	17.643274047520567
13	24.36049450801967	29.577646031527184	27.338572544694152	18.723286915758997
14	19.177351900363067	32.541936669883725	28.299094627510456	19.981616802242748
15	18.81244542488166	26.03520382370513	36.76823383427547	18.384116917137735
16	20.94121972517119	23.92573188106071	35.332506089434254	19.80054230433384
17	21.034054873845307	23.46431361735374	29.1116319683809	26.389999540420057
18	23.514867411186177	25.417528379061537	29.084057171745027	21.983547038007263
19	26.05542534123811	27.252171515235073	26.533388482926608	20.15901466060021
20	29.397490693506136	25.6243393538306	25.869755043889885	19.10841490877338
21	24.93588859782159	26.832115446481914	26.74111861758353	21.490877338112966
22	22.936715841720666	25.944206994806745	27.230111677926377	23.88896548554621
23	23.060802426582104	27.892825957075235	26.13539225148215	22.910979364860516
24	23.04058090904913	25.19325336642309	29.592352589732982	22.1738131347948
25	21.29785376166184	27.26503975366515	28.333103543361364	23.10400294131164
26	20.67098671813962	29.811112643044257	27.759547773335168	21.75835286548095
27	22.231720207730135	28.08401121375063	28.77797692908681	20.90629164943242
28	19.459533985936854	28.237510915023666	31.63380670067558	20.669148398363895
29	21.02578243485454	26.56096327956248	30.347902017555956	22.065352268027024
30	21.8576221333701	26.186865205202448	29.943471666896453	22.012040994531
31	24.377039386001194	26.681373224872466	26.709867181396202	22.231720207730135
32	25.611471115400526	27.58858403419275	25.563674801231674	21.236270049175054
33	24.481823613217518	27.821131485821958	26.35874810423273	21.33829679672779
34	21.41826370697183	27.040764741026702	28.73109977480583	22.809871777195642
35	21.6517303184889	26.157452088790844	28.12629256859231	24.064525024127946
36	23.371478468679626	26.769612574107267	28.426857851923344	21.432051105289766
37	22.353968472815847	27.958086309113472	29.235718553242336	20.452226664828345
38	22.859506411140217	28.288064708856105	25.730961900822646	23.12146697918103
39	23.35769107036169	26.36058642400846	27.7834459304196	22.49827657521026
40	23.310813916080704	25.27505859644285	28.709959097384992	22.704168390091457
41	19.558803253826003	25.907440599292247	28.59598327129004	25.93777287559171
42	22.098442023990074	26.003033227629945	27.39464129785376	24.50388345052622
43	23.407325704306263	26.08667677742543	27.42773105381681	23.07826646445149
44	22.65821039569833	27.536191920584585	27.458982490004136	22.346615193712946
45	22.119582701410913	27.167608805551723	26.574750677880417	24.138057815156948
46	22.83101245461648	27.661197665333887	27.322946826600486	22.184843053449146
47	21.660921917367524	26.333930787260446	29.055563215221287	22.949584080150743
48	23.084700583666528	24.646353233144904	28.409393814053956	23.85955236913461
49	21.672870995909737	24.54800312514362	29.059239854772738	24.719886024173903
50	20.314352681648973	26.492945447860656	29.179649800082725	24.013052070407646
51	20.760145227262285	26.50857116595432	26.650121788685144	26.08116181809826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	143.5
2	226.0
3	360.0
4	494.0
5	361.0
6	228.0
7	219.5
8	211.0
9	208.5
10	206.0
11	226.5
12	247.0
13	240.5
14	234.0
15	249.5
16	265.0
17	255.5
18	246.0
19	274.0
20	302.0
21	324.5
22	347.0
23	420.5
24	494.0
25	618.5
26	897.5
27	1052.0
28	1266.5
29	1481.0
30	1728.0
31	1975.0
32	2209.0
33	2443.0
34	2644.5
35	2846.0
36	3110.0
37	3374.0
38	3796.5
39	4219.0
40	4960.0
41	5701.0
42	6523.5
43	7346.0
44	8063.0
45	8780.0
46	9255.5
47	9731.0
48	9658.5
49	9586.0
50	9248.0
51	8910.0
52	8191.0
53	7472.0
54	6686.0
55	5900.0
56	5426.5
57	4953.0
58	4776.0
59	4599.0
60	4546.0
61	4493.0
62	3955.0
63	3417.0
64	2806.5
65	2196.0
66	1852.0
67	1508.0
68	1219.5
69	931.0
70	769.0
71	607.0
72	511.0
73	415.0
74	339.0
75	207.0
76	151.0
77	108.0
78	65.0
79	51.0
80	37.0
81	27.0
82	17.0
83	13.0
84	9.0
85	6.0
86	3.0
87	4.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	108795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.08580357553196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.58244675659321	32.76345420285859
2	10.129423936595936	9.53904131623696
3	5.520526284966912	7.798152488625397
4	3.722646260760927	7.011351624615101
5	2.6392332168582975	6.213520841950458
6	2.0692213090752922	5.84585688680546
7	1.479688445546294	4.877062364998391
8	1.1732094403341988	4.41932074084287
9	0.9331016846584809	3.9542258375844477
>10	2.725125422140668	16.555907900179236
>50	0.019520955745993322	0.6333011627372581
>100	0.005856286723797997	0.38880463256583486
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.18199365779677376	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC	124	0.1139758260949492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12224826508571165	0.0
2	0.0	0.0	0.0	0.5248402959694839	0.0
3	0.0	0.0	0.0	0.6856932763454203	0.0
4	0.0	0.0	0.0	0.9191598878624937	0.0
5	0.0	0.0	0.0	1.6039340043200514	0.0
6	0.0	0.0	0.0	2.011121834643136	0.0
7	0.0	0.0	0.0	2.4330162231720207	0.0
8	0.0	0.0	0.0	3.3163288754078772	0.0
9	0.0	0.0	0.0	3.6821545107771496	0.0
10	0.0	0.0	0.0	4.607748517854681	0.0
11	0.0	0.0	0.0	5.618824394503424	0.0
12	0.0	0.0	0.0	6.481915529206305	0.0
13	0.0	0.0	0.0	6.848660324463441	0.0
14	0.0	0.0	0.0	6.991130107082127	0.0
15	0.0	0.0	0.0	7.230111677926375	0.0
16	0.0	0.0	0.0	7.75127533434441	0.0
17	0.0	0.0	0.0	8.405717174502504	0.0
18	0.0	0.0	0.0	9.117146927708076	0.0
19	0.0	0.0	0.0	9.51698147892826	0.0
20	0.0	0.0	0.0	9.891079553288295	0.0
21	0.0	0.0	0.0	10.354336136770991	0.0
22	0.0	0.0	0.0	10.91410450847925	0.0
23	0.0	0.0	0.0	11.418723286915759	0.0
24	0.0	0.0	0.0	11.839698515556782	0.0
25	0.0	0.0	0.0	12.15680867686934	0.0
26	0.0	0.0	0.0	12.51711935291144	0.0
27	0.0	0.0	0.0	12.859965991084149	0.0
28	0.0	0.0	0.0	13.205570108920448	0.0
29	0.0	0.0	0.0	13.569557424513993	0.0
30	0.0	0.0	0.0	13.996047612482192	0.0
31	0.0	0.0	0.0	14.405073762581	0.0
32	0.0	0.0	0.0	14.779171836941035	0.0
33	0.0	0.0	0.0	15.12753343444092	0.0
34	0.0	0.0	0.0	15.482329151155843	0.0
35	0.0	0.0	0.0	15.905142699572592	0.0
36	0.0	0.0	0.0	16.322441288662162	0.0
37	0.0	0.0	0.0	16.702973482237237	0.0
38	0.0	0.0	0.0	17.055930879176433	0.0
39	0.0	0.0	0.0	17.47506778804173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	30	2.1473552E-6	45.000004	7
GCGGGTA	20	7.005117E-4	45.000004	5
TCACTTA	20	7.005117E-4	45.000004	5
GTCGGCA	20	7.005117E-4	45.000004	20
AGTCGAG	20	7.005117E-4	45.000004	31
CGTGCAT	25	3.8674756E-5	45.0	17
GGCACCG	25	3.8674756E-5	45.0	8
ATAGCGG	35	1.1984048E-7	45.0	2
CACGCGC	25	3.8674756E-5	45.0	11
TATGCGC	25	3.8674756E-5	45.0	23
AATATGC	25	3.8674756E-5	45.0	40
GCGTGCA	25	3.8674756E-5	45.0	16
CAGACAC	25	3.8674756E-5	45.0	32
ATATGGG	50	1.0622898E-9	40.5	3
TAGCGGG	45	1.900844E-8	40.0	3
TATGCGG	40	3.419882E-7	39.375004	2
CGAAGGA	40	3.419882E-7	39.375004	4
CGTTTTT	115	0.0	39.130436	1
GCGAAGG	35	6.1962764E-6	38.571426	3
GCGGGAA	35	6.1962764E-6	38.571426	5
>>END_MODULE