##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207912
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.982290584478047	33.0	31.0	34.0	30.0	34.0
2	32.07670071953519	33.0	31.0	34.0	30.0	34.0
3	31.94757878333141	33.0	31.0	34.0	30.0	34.0
4	35.69853110931548	37.0	35.0	37.0	35.0	37.0
5	35.78313421062757	37.0	35.0	37.0	35.0	37.0
6	35.72573492631498	37.0	35.0	37.0	35.0	37.0
7	26.650126014852436	35.0	10.0	37.0	0.0	37.0
8	30.92160625649313	35.0	19.0	37.0	17.0	37.0
9	35.741313632690755	38.0	32.0	39.0	32.0	39.0
10	36.64870714533072	37.0	35.0	39.0	33.0	39.0
11	36.97834660818038	39.0	37.0	39.0	33.0	39.0
12	36.93322174766247	39.0	37.0	39.0	33.0	39.0
13	36.84002847358498	39.0	35.0	39.0	33.0	39.0
14	38.11724671976606	40.0	37.0	41.0	33.0	41.0
15	38.084992689214666	40.0	37.0	41.0	33.0	41.0
16	38.09609834930163	40.0	37.0	41.0	33.0	41.0
17	37.915925968679055	40.0	37.0	41.0	33.0	41.0
18	37.76125476162992	40.0	36.0	41.0	33.0	41.0
19	37.62739524414175	40.0	36.0	41.0	32.0	41.0
20	37.75980703374505	40.0	36.0	41.0	33.0	41.0
21	37.79113759667552	40.0	36.0	41.0	33.0	41.0
22	37.86258609411674	40.0	36.0	41.0	33.0	41.0
23	37.85566489668706	40.0	36.0	41.0	33.0	41.0
24	37.781850013467235	40.0	36.0	41.0	33.0	41.0
25	37.69585209126938	40.0	36.0	41.0	33.0	41.0
26	37.49656585478472	39.0	36.0	41.0	33.0	41.0
27	37.45383142868137	39.0	36.0	41.0	33.0	41.0
28	37.412227288468195	39.0	36.0	41.0	33.0	41.0
29	37.333886451960446	39.0	36.0	41.0	32.0	41.0
30	37.27709319327408	39.0	35.0	41.0	32.0	41.0
31	37.136221093539575	39.0	35.0	41.0	32.0	41.0
32	37.01899842240948	39.0	35.0	41.0	31.0	41.0
33	36.896537958367	39.0	35.0	41.0	31.0	41.0
34	36.66240043864712	39.0	35.0	41.0	30.0	41.0
35	36.63951094693909	39.0	35.0	41.0	30.0	41.0
36	36.21832313671169	39.0	35.0	41.0	30.0	41.0
37	36.34683423756204	39.0	35.0	41.0	30.0	41.0
38	36.16887914117511	39.0	35.0	40.0	30.0	41.0
39	36.1440513294086	39.0	35.0	40.0	30.0	41.0
40	35.991679171957365	39.0	35.0	40.0	29.0	41.0
41	35.655921736119126	38.0	35.0	40.0	27.0	41.0
42	35.69924294893993	38.0	35.0	40.0	28.0	41.0
43	35.53553907422371	38.0	35.0	40.0	27.0	41.0
44	35.48577282696525	38.0	35.0	40.0	27.0	41.0
45	35.4034495363423	38.0	34.0	40.0	27.0	41.0
46	35.175295317249606	38.0	34.0	40.0	26.0	41.0
47	34.92794547693255	38.0	34.0	40.0	24.0	41.0
48	34.754819346646656	38.0	34.0	40.0	24.0	41.0
49	34.55491746508138	37.0	33.0	40.0	23.0	41.0
50	34.41290065027512	37.0	33.0	40.0	23.0	41.0
51	32.06522471045442	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	18.0
10	8.0
11	6.0
12	4.0
13	6.0
14	15.0
15	21.0
16	23.0
17	65.0
18	120.0
19	195.0
20	269.0
21	412.0
22	593.0
23	834.0
24	1257.0
25	1806.0
26	2249.0
27	2665.0
28	3145.0
29	3707.0
30	4557.0
31	5884.0
32	7663.0
33	10131.0
34	15192.0
35	17973.0
36	19997.0
37	30518.0
38	38000.0
39	40567.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.87223440686444	16.66714763938589	20.61112393705029	15.849494016699373
2	28.130170456731697	30.093501096617796	24.513255608141908	17.2630728385086
3	30.35659317403517	29.148389703336026	25.199603678479356	15.295413444149448
4	27.673727346184922	23.459925353033974	30.548020316287662	18.31832698449344
5	28.690984647350803	27.48278117665166	24.12751548732156	19.698718688675978
6	25.803705413828926	36.20522143983993	24.38291200123129	13.608161145099851
7	57.18428950709916	27.958463195967525	10.680480203162876	4.176767093770441
8	76.18848358921082	9.315479625995614	9.843106698988032	4.652930085805533
9	70.54859748355074	7.508946092577629	10.843048982261726	11.099407441609912
10	41.260725691638775	24.893705029050754	18.361614529223903	15.483954750086577
11	33.52139366655123	23.918292354457655	24.606564315672014	17.953749663319098
12	30.03289853399515	21.998249259302014	28.15806687444688	19.810785332255954
13	24.126553541883105	23.72590326676671	32.57772519142714	19.569817999923046
14	21.126726692062025	27.478452422178613	28.93579976143753	22.45902112432183
15	19.64004001693024	24.330966947554735	36.9516911000808	19.07730193543422
16	23.346896764015547	25.261649159259687	29.141656085266842	22.249797991457925
17	23.513313324868214	24.364154065181424	29.663511485628536	22.45902112432183
18	23.646542768094196	25.25347262303282	29.21332102043172	21.886663588441262
19	24.428604409557888	27.05038670206626	26.06631651852707	22.454692369848782
20	26.82144368771403	25.959059602139366	29.187348493593447	18.032148216553157
21	25.373715802839662	28.19654469198507	27.13984762784255	19.289891877332717
22	23.787948747547038	24.08230405171419	27.68815652776175	24.44159067297703
23	23.3021663011274	28.145080611027744	28.438473969756433	20.114279118088422
24	25.136115279541343	23.056870214321442	27.079245065219904	24.72776944091731
25	22.826003309092307	29.535572742314052	25.594001308245794	22.04442264034784
26	22.30703374504598	26.342394859363576	27.036438493208664	24.314132902381775
27	23.932721536034478	27.355804378775634	25.839778367770982	22.87169571741891
28	20.534168301973914	27.261533725807073	29.588960714148293	22.615337258070724
29	24.876390011158566	26.926295740505594	26.335180268575165	21.862133979760667
30	24.96536996421563	26.256300742621878	27.56166070260495	21.216668590557543
31	25.132267497787524	27.375524260263955	25.206818269267767	22.28538997268075
32	27.982511831928893	26.903690022701916	26.489091538727923	18.624706606641272
33	26.666089499403594	25.54446111816538	24.83935511177806	22.95009427065297
34	26.138462426411174	27.432760013852015	27.28173458001462	19.14704297972219
35	27.157162645734733	25.5079071915041	26.083631536419254	21.251298626341914
36	24.268921466774405	30.17334256800954	26.781041979298937	18.77669398591712
37	24.85186040247797	26.7137057986071	28.836719381276694	19.597714417638233
38	24.807129939589824	27.763188271961216	25.071183962445648	22.35849782600331
39	26.60356304590404	24.178017622840432	28.484166378083035	20.734252953172497
40	28.32111662626496	24.20928084959021	27.907480087729425	19.562122436415407
41	22.746161837700566	26.977759821462925	28.257628227326947	22.018450113509562
42	23.887510100427104	24.964408018777178	29.881392127438534	21.26668975335719
43	24.555581207433914	24.80087729423987	27.247585516949478	23.395955981376737
44	24.40840355535034	24.72969333179422	27.392358305436915	23.469544807418526
45	22.76732463734657	24.345877101850782	27.22642271730347	25.660375543499175
46	24.73161722267113	27.451998922621108	25.48578244641964	22.33060140828812
47	21.20272038169995	25.160163915502714	32.52385624687368	21.11325945592366
48	23.998614798568628	25.180364769710263	27.061930047327714	23.759090384393396
49	21.346050252029706	24.94180230097349	30.802454884758934	22.909692562237872
50	21.61539497479703	25.306379622147833	28.842010081188196	24.236215321866943
51	22.24306437338874	24.77538574012082	26.742083189041516	26.239466697448922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	198.5
2	345.0
3	911.5
4	1478.0
5	1056.0
6	634.0
7	554.5
8	475.0
9	465.5
10	456.0
11	436.5
12	417.0
13	422.5
14	428.0
15	469.0
16	510.0
17	540.0
18	570.0
19	603.0
20	636.0
21	629.0
22	622.0
23	775.0
24	928.0
25	1085.5
26	1538.5
27	1834.0
28	2277.0
29	2720.0
30	3182.0
31	3644.0
32	4050.5
33	4457.0
34	5166.5
35	5876.0
36	6277.5
37	6679.0
38	7112.0
39	7545.0
40	8412.5
41	9280.0
42	10190.0
43	11100.0
44	12237.0
45	13374.0
46	19119.5
47	24865.0
48	19948.0
49	15031.0
50	14885.5
51	14740.0
52	13777.5
53	12815.0
54	12171.5
55	11528.0
56	11017.5
57	10507.0
58	10262.0
59	10017.0
60	9730.0
61	9443.0
62	8493.0
63	7543.0
64	6575.0
65	5607.0
66	4673.5
67	3740.0
68	3098.5
69	2457.0
70	2080.0
71	1703.0
72	1382.5
73	1062.0
74	865.5
75	569.0
76	469.0
77	340.0
78	211.0
79	153.5
80	96.0
81	68.0
82	40.0
83	36.0
84	32.0
85	24.5
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	207912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.39847627842549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.88746527345856	31.034764708145758
2	12.147033213086205	11.272076647812536
3	6.8095119625160665	9.478529377813691
4	4.344445826595348	8.063026665127554
5	2.992702243230916	6.9428412020470205
6	2.0493842517726084	5.705298395475008
7	1.4564415142845295	4.730366693601138
8	1.0303934983621512	3.8246950632960095
9	0.7038603474727371	2.9392242871984298
>10	1.54144379483352	8.98745623148255
>50	0.016585810838827385	0.530512909307784
>100	0.012439358129120536	1.4198314671591827
>500	0.005183065887133557	1.4554234483820077
>1k	0.003109839532280134	3.615952903151333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	3000	1.4429181576820964	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	2273	1.0932509908038017	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	2245	1.0797837546654354	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	717	0.344857439686021	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.31792296740928855	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	556	0.26742083189041516	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	549	0.26405402285582363	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	543	0.2611681865404594	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	490	0.23567663242140907	No Hit
GAATCTATCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	458	0.22028550540613337	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTT	412	0.19816076032167454	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	383	0.18421255146408097	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	226	0.1086998345453846	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	213	0.10244718919542883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.809727192273654E-4	0.0	0.0	0.08561314402247104	0.0
2	4.809727192273654E-4	0.0	0.0	0.6858670976182231	0.0
3	4.809727192273654E-4	0.0	0.0	0.8239062680364769	0.0
4	4.809727192273654E-4	0.0	0.0	1.2928546692831582	0.0
5	4.809727192273654E-4	0.0	0.0	3.098426257262688	0.0
6	4.809727192273654E-4	0.0	0.0	3.513024741236677	0.0
7	4.809727192273654E-4	0.0	0.0	4.0565239139636	0.0
8	4.809727192273654E-4	0.0	0.0	4.669764130978491	0.0
9	4.809727192273654E-4	0.0	0.0	4.874658509369349	0.0
10	4.809727192273654E-4	0.0	0.0	7.229981915425757	0.0
11	4.809727192273654E-4	0.0	0.0	7.730674516141445	0.0
12	4.809727192273654E-4	0.0	0.0	9.565585439993843	0.0
13	4.809727192273654E-4	0.0	0.0	9.764227173034746	0.0
14	4.809727192273654E-4	0.0	0.0	9.91332871599523	0.0
15	4.809727192273654E-4	0.0	0.0	10.204798183847013	0.0
16	4.809727192273654E-4	0.0	0.0	10.495786678979568	0.0
17	4.809727192273654E-4	0.0	0.0	10.783408365077532	0.0
18	4.809727192273654E-4	0.0	0.0	11.103736196082957	0.0
19	4.809727192273654E-4	0.0	0.0	11.305263765439225	0.0
20	4.809727192273654E-4	0.0	0.0	11.522182461810766	0.0
21	4.809727192273654E-4	0.0	0.0	11.781426757474316	0.0
22	4.809727192273654E-4	0.0	0.0	12.044037862172457	0.0
23	4.809727192273654E-4	0.0	0.0	12.301358266959099	0.0
24	4.809727192273654E-4	0.0	0.0	12.526453499557505	0.0
25	4.809727192273654E-4	0.0	0.0	12.727500096194543	0.0
26	4.809727192273654E-4	0.0	0.0	12.943937819846859	0.0
27	4.809727192273654E-4	0.0	0.0	13.159894570779946	0.0
28	4.809727192273654E-4	0.0	0.0	13.360941167416984	0.0
29	4.809727192273654E-4	0.0	0.0	13.571126245719343	0.0
30	4.809727192273654E-4	0.0	0.0	13.838547077609759	0.0
31	4.809727192273654E-4	0.0	0.0	14.045365346877524	0.0
32	4.809727192273654E-4	0.0	0.0	14.302204778944938	0.0
33	4.809727192273654E-4	0.0	0.0	14.540767247681712	0.0
34	4.809727192273654E-4	0.0	0.0	14.765862480280118	0.0
35	4.809727192273654E-4	0.0	0.0	15.003943976297665	0.0
36	4.809727192273654E-4	0.0	0.0	15.237696717842164	0.0
37	4.809727192273654E-4	0.0	0.0	15.487802531840394	0.0
38	4.809727192273654E-4	0.0	0.0	15.763880872676902	0.0
39	4.809727192273654E-4	0.0	0.0	16.10777636692447	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGGG	20	7.019945E-4	45.000004	3
GCTACGA	20	7.019945E-4	45.000004	10
CGGGTAT	25	3.8797523E-5	45.0	6
GCGATTG	25	3.8797523E-5	45.0	9
TTGGGTA	25	3.8797523E-5	45.0	5
TTAATCG	30	2.1568994E-6	44.999996	20
AATCGTT	30	2.1568994E-6	44.999996	22
TAATCGT	30	2.1568994E-6	44.999996	21
TGATACC	335	0.0	43.656715	4
TACGGCT	300	0.0	42.75	7
ACGGCTG	360	0.0	42.5	8
GACACGA	65	0.0	41.53846	25
AAACACG	65	0.0	41.53846	40
TCAAGCG	65	0.0	41.53846	17
TGGTCAA	65	0.0	41.53846	14
ACGACCA	65	0.0	41.53846	28
AACACGT	65	0.0	41.53846	41
AAACGGC	60	3.6379788E-12	41.249996	6
AACGGCT	60	3.6379788E-12	41.249996	7
ATGAAAC	155	0.0	40.64516	3
>>END_MODULE