##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192791
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06384115441074	33.0	31.0	34.0	30.0	34.0
2	32.178084039192704	33.0	31.0	34.0	30.0	34.0
3	32.10647281252756	33.0	31.0	34.0	30.0	34.0
4	35.819789305517375	37.0	35.0	37.0	35.0	37.0
5	35.88065314252221	37.0	35.0	37.0	35.0	37.0
6	35.85006042813202	37.0	35.0	37.0	35.0	37.0
7	26.697060547432194	35.0	10.0	37.0	0.0	37.0
8	31.088873443262393	35.0	19.0	37.0	17.0	37.0
9	35.971025618415794	39.0	32.0	39.0	32.0	39.0
10	36.72266858930137	38.0	35.0	39.0	33.0	39.0
11	37.048176522762994	39.0	37.0	39.0	34.0	39.0
12	37.059940557391165	39.0	37.0	39.0	34.0	39.0
13	36.9926863805883	39.0	37.0	39.0	33.0	39.0
14	38.1720308520626	40.0	37.0	41.0	33.0	41.0
15	38.25502746497502	40.0	37.0	41.0	33.0	41.0
16	38.22916007489976	40.0	37.0	41.0	33.0	41.0
17	38.126344072078055	40.0	37.0	41.0	33.0	41.0
18	38.04073841621238	40.0	37.0	41.0	33.0	41.0
19	37.91886032024316	40.0	37.0	41.0	33.0	41.0
20	38.022366189293066	40.0	37.0	41.0	34.0	41.0
21	37.94165702755834	40.0	36.0	41.0	33.0	41.0
22	37.93863302747535	40.0	36.0	41.0	33.0	41.0
23	37.89928471764761	40.0	36.0	41.0	33.0	41.0
24	37.84085875378	40.0	36.0	41.0	33.0	41.0
25	37.74841667920183	40.0	36.0	41.0	33.0	41.0
26	37.59292705572356	40.0	36.0	41.0	33.0	41.0
27	37.51571909477102	40.0	36.0	41.0	33.0	41.0
28	37.466935697205784	40.0	36.0	41.0	33.0	41.0
29	37.47739780383939	40.0	36.0	41.0	33.0	41.0
30	37.37791183198386	40.0	36.0	41.0	32.0	41.0
31	37.270775088048715	39.0	35.0	41.0	32.0	41.0
32	37.08849998184562	39.0	35.0	41.0	31.0	41.0
33	36.98378036319123	39.0	35.0	41.0	31.0	41.0
34	36.85329190677988	39.0	35.0	41.0	31.0	41.0
35	36.767307602533315	39.0	35.0	41.0	31.0	41.0
36	36.62468165007703	39.0	35.0	41.0	30.0	41.0
37	36.49831164317836	39.0	35.0	41.0	30.0	41.0
38	36.29282487253035	39.0	35.0	41.0	30.0	41.0
39	36.19909643084999	39.0	35.0	40.0	30.0	41.0
40	36.009979718970285	39.0	35.0	40.0	29.0	41.0
41	35.96922055490142	39.0	35.0	40.0	29.0	41.0
42	35.890057108474984	38.0	35.0	40.0	28.0	41.0
43	35.810359404744	38.0	35.0	40.0	28.0	41.0
44	35.6189085590095	38.0	35.0	40.0	27.0	41.0
45	35.44307047528152	38.0	34.0	40.0	27.0	41.0
46	35.301160323874036	38.0	34.0	40.0	26.0	41.0
47	35.173955215751775	38.0	34.0	40.0	26.0	41.0
48	35.09927849329066	38.0	34.0	40.0	26.0	41.0
49	34.950023600686755	38.0	34.0	40.0	26.0	41.0
50	34.74509183519977	37.0	34.0	40.0	24.0	41.0
51	32.699368746466384	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	13.0
11	8.0
12	4.0
13	4.0
14	6.0
15	13.0
16	32.0
17	39.0
18	70.0
19	134.0
20	227.0
21	375.0
22	545.0
23	762.0
24	1149.0
25	1538.0
26	2008.0
27	2300.0
28	2743.0
29	3138.0
30	3826.0
31	4630.0
32	6302.0
33	8571.0
34	13611.0
35	16960.0
36	18512.0
37	29503.0
38	36762.0
39	38981.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.249990922812785	17.75342209957934	23.523919685047538	14.472667292560338
2	29.215575415864848	26.021442909679394	26.246038456152	18.51694321830376
3	32.21830894595702	25.2864500936247	26.205061439590022	16.290179520828254
4	28.194262180288497	26.085242568377154	26.18690706516383	19.533588186170515
5	26.12829437058784	30.8323521326203	23.994377330892004	19.04497616589986
6	26.62053726574373	35.547302519308474	24.187332396221812	13.644827818725977
7	62.78456981913057	28.00597538266828	5.698917480587787	3.510537317613374
8	85.1512778086114	5.383550061984222	6.195828643453273	3.2693434859511075
9	79.61834317992023	7.03248595629464	7.780964879065931	5.568205984719204
10	42.51702620972971	28.769496501392698	14.466442935614216	14.24703435326338
11	32.36406263777874	25.945713233501564	23.805053140447427	17.885170988272275
12	28.037615863811073	24.906764319911197	28.24509442868184	18.810525387595895
13	23.698201679538982	26.901152024731445	29.867576805971236	19.53306948975834
14	21.538868515646477	28.420413815997637	29.507601495920454	20.533116172435438
15	20.402404676566853	25.305641860875248	33.7826973250826	20.509256137475298
16	24.3652452655985	25.46384426658921	29.99932569466417	20.171584773148123
17	24.951372211358414	24.994942709981274	26.975325611672744	23.078359466987568
18	26.276122848058257	24.194075449580115	28.15069168166564	21.379110020695986
19	25.524531746813906	25.980984589529594	26.339403810343843	22.155079853312653
20	27.62473351971824	26.1516357091358	26.51627928689617	19.707351484249784
21	25.679621974054808	27.24349165676821	27.630439180252193	19.446447188924797
22	25.986171553651367	23.605355021759316	27.58686868162933	22.82160474295999
23	23.762001338236743	27.828581209703774	27.176579819597386	21.232837632462097
24	23.700276465187688	25.156257294168295	29.31153425211758	21.831931988526435
25	22.846502170744483	28.092078987089646	27.29069303027631	21.77072581188956
26	23.570083665731286	27.917278296186026	26.335772935458607	22.176865102624085
27	22.59493441083868	27.05001789502622	27.11070537525092	23.244342318884183
28	20.177809130094246	27.464456328355574	29.373777821578805	22.983956719971367
29	22.272305242464636	26.085242568377154	28.19374348387632	23.448708705281884
30	23.16342567858458	26.03233553433511	28.36698808554341	22.437250701536897
31	24.74700582496071	26.437956128657458	25.9021427348787	22.91289531150313
32	25.57225181673418	26.897002453434027	26.35859557759439	21.172150152237396
33	23.741253481749666	27.310403493939035	25.417680285905465	23.530662738405837
34	22.21317385147647	27.307810011878146	28.262730106695855	22.21628602994953
35	21.462101446644294	27.896530439698946	28.332754122339736	22.30861399131702
36	23.92954027936989	28.687023771856566	26.031298141510756	21.352137807262785
37	23.588756736569653	28.216566126012104	25.997064178307077	22.19761295911116
38	22.66910799777998	28.467096493093557	26.317099864620236	22.546695644506226
39	23.02234025447246	28.387217245618313	25.94519453708939	22.64524796281984
40	24.487657618872248	26.733094387186124	27.183841569367868	21.595406424573763
41	20.96830246225187	28.060438505946856	26.558293696282504	24.412965335518773
42	23.306067191933234	25.692589384359227	27.89497435046242	23.10636907324512
43	22.784258601283256	26.367413416601398	26.967026469077915	23.881301513037435
44	22.792557743878085	26.350815131411736	26.742949619017487	24.113677505692692
45	22.578336125649017	27.248159924477804	26.245001063327646	23.928502886545534
46	23.30969806681847	27.51580727316109	26.29116504401139	22.883329616009046
47	21.434091840386742	27.672972286050694	28.4728021536275	22.42013371993506
48	22.562256536871534	27.057279644796694	27.532405558350753	22.848058259981016
49	22.936236650051093	26.414614790109496	28.285034052419462	22.364114507419952
50	21.58347640709369	27.97018533022807	27.890306082752826	22.55603217992541
51	21.95382564538801	26.628317711926385	25.980984589529594	25.436872053156005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	171.5
2	310.0
3	490.0
4	670.0
5	505.0
6	340.0
7	327.0
8	314.0
9	301.0
10	288.0
11	295.5
12	303.0
13	315.0
14	327.0
15	313.5
16	300.0
17	345.0
18	390.0
19	445.5
20	501.0
21	569.0
22	637.0
23	797.5
24	958.0
25	1104.5
26	1530.5
27	1810.0
28	2201.5
29	2593.0
30	3134.5
31	3676.0
32	4063.0
33	4450.0
34	5156.0
35	5862.0
36	6485.5
37	7109.0
38	7647.5
39	8186.0
40	9026.5
41	9867.0
42	10640.5
43	11414.0
44	12378.0
45	13342.0
46	14531.5
47	15721.0
48	15205.0
49	14689.0
50	14950.5
51	15212.0
52	14240.0
53	13268.0
54	12559.0
55	11850.0
56	11082.0
57	10314.0
58	9680.0
59	9046.0
60	8399.0
61	7752.0
62	6919.0
63	6086.0
64	5270.0
65	4454.0
66	3869.0
67	3284.0
68	2742.0
69	2200.0
70	1823.0
71	1446.0
72	1229.0
73	1012.0
74	852.0
75	563.0
76	434.0
77	328.5
78	223.0
79	155.0
80	87.0
81	62.5
82	38.0
83	28.0
84	18.0
85	15.0
86	12.0
87	10.5
88	9.0
89	6.5
90	4.0
91	3.5
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.38324921806516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.21837898587978	28.29385189142647
2	10.77726897739093	9.351058918725458
3	6.463491911668001	8.41221841268524
4	4.464424490967132	7.747249612274432
5	3.186312605363527	6.911629692257419
6	2.27047190903765	5.9100269203437925
7	1.9452647688279392	5.907433438282907
8	1.435932997764201	4.983635128195818
9	1.1453986776503784	4.472200465789379
>10	3.0715336147012757	16.667271812480873
>50	0.015542988318846472	0.48394375256106353
>100	0.005978072430325566	0.8594799549771515
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG	476	0.24689949219621246	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	415	0.21525901105342052	RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC	362	0.18776810120804394	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.1431602097608291	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038902230913268776	0.0
2	0.0	0.0	0.0	0.24482470654750482	0.0
3	0.0	0.0	0.0	0.3070682760087349	0.0
4	0.0	0.0	0.0	0.44815370012085626	0.0
5	0.0	0.0	0.0	1.0332432530564186	0.0
6	0.0	0.0	0.0	1.2780679596039235	0.0
7	0.0	0.0	0.0	1.5405283441654434	0.0
8	0.0	0.0	0.0	2.00372424023943	0.0
9	0.0	0.0	0.0	2.200828876866659	0.0
10	0.0	0.0	0.0	3.0084391906261185	0.0
11	0.0	0.0	0.0	3.4752659615853436	0.0
12	0.0	0.0	0.0	4.1506086902396895	0.0
13	0.0	0.0	0.0	4.326965470379841	0.0
14	0.0	0.0	0.0	4.407882110679441	0.0
15	0.0	0.0	0.0	4.553635802501154	0.0
16	0.0	0.0	0.0	4.824914026069682	0.0
17	0.0	0.0	0.0	5.130426212841885	0.0
18	0.0	0.0	0.0	5.509074593731035	0.0
19	0.0	0.0	0.0	5.719146640662687	0.0
20	0.0	0.0	0.0	5.9307747768308685	0.0
21	0.0	0.0	0.0	6.193235161392389	0.0
22	0.0	0.0	0.0	6.488892116333231	0.0
23	0.0	0.0	0.0	6.758614250665228	0.0
24	0.0	0.0	0.0	6.974910654543002	0.0
25	0.0	0.0	0.0	7.189650969184246	0.0
26	0.0	0.0	0.0	7.419952176190797	0.0
27	0.0	0.0	0.0	7.639360758541633	0.0
28	0.0	0.0	0.0	7.8442458413515155	0.0
29	0.0	0.0	0.0	8.077659226831129	0.0
30	0.0	0.0	0.0	8.367091824825847	0.0
31	0.0	0.0	0.0	8.59220606771063	0.0
32	0.0	0.0	0.0	8.835474685021603	0.0
33	0.0	0.0	0.0	9.062663713555093	0.0
34	0.0	0.0	0.0	9.314231473460898	0.0
35	0.0	0.0	0.0	9.60988842840174	0.0
36	5.18696412176917E-4	0.0	0.0	9.865087063192783	0.0
37	5.18696412176917E-4	0.0	0.0	10.093313484550627	0.0
38	5.18696412176917E-4	0.0	0.0	10.358367351173031	0.0
39	5.18696412176917E-4	0.0	0.0	10.754132713664019	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	30	2.1560754E-6	45.000004	3
AAGGCGA	30	2.1560754E-6	45.000004	6
TGATCGT	20	7.0186664E-4	45.0	29
TAAGGAT	40	6.7648216E-9	45.0	5
TGCGTTG	20	7.0186664E-4	45.0	1
GCGAGAC	80	0.0	39.375	21
ACACGAC	80	0.0	39.375	26
CATGCGG	40	3.4382902E-7	39.375	2
CGCGCAA	40	3.4382902E-7	39.375	18
CACGACC	80	0.0	39.375	27
CGCAATC	40	3.4382902E-7	39.375	20
ACACGTG	75	0.0	39.0	42
AGGCGAT	75	0.0	39.0	7
TCATGAT	35	6.2212694E-6	38.57143	26
TTACGGG	35	6.2212694E-6	38.57143	3
CGTTTTA	35	6.2212694E-6	38.57143	1
ATGGGAA	170	0.0	38.38235	5
CATGGGA	130	0.0	38.07692	4
CGGTAGT	95	0.0	37.894737	12
GATCGGG	30	1.13640024E-4	37.500004	3
>>END_MODULE