##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936519.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182065
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01617554170214	33.0	31.0	34.0	30.0	34.0
2	32.13389174196029	33.0	31.0	34.0	30.0	34.0
3	32.0765495839398	33.0	31.0	34.0	30.0	34.0
4	35.79523247191937	37.0	35.0	37.0	35.0	37.0
5	35.8557108724906	37.0	35.0	37.0	35.0	37.0
6	35.81601625792986	37.0	35.0	37.0	35.0	37.0
7	26.724801581852635	35.0	10.0	37.0	0.0	37.0
8	31.11167989454316	35.0	19.0	37.0	17.0	37.0
9	35.93847252354928	39.0	32.0	39.0	32.0	39.0
10	36.681421470353996	38.0	35.0	39.0	33.0	39.0
11	37.05627660450938	39.0	37.0	39.0	34.0	39.0
12	37.078411556312304	39.0	37.0	39.0	34.0	39.0
13	37.03280147200176	39.0	37.0	39.0	33.0	39.0
14	38.20082388158075	40.0	37.0	41.0	33.0	41.0
15	38.3019306291709	40.0	37.0	41.0	34.0	41.0
16	38.24429736632521	40.0	37.0	41.0	34.0	41.0
17	38.1698459341444	40.0	37.0	41.0	34.0	41.0
18	38.06912366462527	40.0	37.0	41.0	33.0	41.0
19	37.97234504160602	40.0	37.0	41.0	33.0	41.0
20	38.02730343558619	40.0	37.0	41.0	34.0	41.0
21	37.953598989371926	40.0	36.0	41.0	33.0	41.0
22	37.936742372229695	40.0	37.0	41.0	33.0	41.0
23	37.88266827781287	40.0	36.0	41.0	33.0	41.0
24	37.839985719385936	40.0	36.0	41.0	33.0	41.0
25	37.724257820009335	40.0	36.0	41.0	33.0	41.0
26	37.58903138988823	40.0	36.0	41.0	33.0	41.0
27	37.47316617691484	40.0	36.0	41.0	33.0	41.0
28	37.442028945706205	40.0	36.0	41.0	33.0	41.0
29	37.436305714991896	40.0	36.0	41.0	33.0	41.0
30	37.34567324856507	40.0	36.0	41.0	32.0	41.0
31	37.240793123335074	39.0	35.0	41.0	32.0	41.0
32	37.121462115178645	39.0	35.0	41.0	32.0	41.0
33	36.993705544723035	39.0	35.0	41.0	31.0	41.0
34	36.85997308653503	39.0	35.0	41.0	31.0	41.0
35	36.78633455084722	39.0	35.0	41.0	31.0	41.0
36	36.6496745667756	39.0	35.0	41.0	30.0	41.0
37	36.59386482849532	39.0	35.0	41.0	30.0	41.0
38	36.48092164886167	39.0	35.0	41.0	30.0	41.0
39	36.399796775876744	39.0	35.0	41.0	30.0	41.0
40	36.16768736440282	39.0	35.0	40.0	30.0	41.0
41	36.097190563809626	39.0	35.0	40.0	29.0	41.0
42	36.03877186719029	38.0	35.0	40.0	29.0	41.0
43	36.02459561145745	38.0	35.0	40.0	29.0	41.0
44	35.83670667069453	38.0	35.0	40.0	29.0	41.0
45	35.721874055969025	38.0	35.0	40.0	28.0	41.0
46	35.571037816164555	38.0	34.0	40.0	28.0	41.0
47	35.46232389531211	38.0	34.0	40.0	27.0	41.0
48	35.39345838024881	38.0	34.0	40.0	27.0	41.0
49	35.20976574300387	38.0	34.0	40.0	26.0	41.0
50	34.95672424683492	38.0	34.0	40.0	26.0	41.0
51	32.76829703677258	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	7.0
10	17.0
11	8.0
12	12.0
13	9.0
14	11.0
15	20.0
16	21.0
17	51.0
18	69.0
19	139.0
20	235.0
21	349.0
22	453.0
23	707.0
24	994.0
25	1341.0
26	1672.0
27	2008.0
28	2430.0
29	2829.0
30	3463.0
31	4549.0
32	5833.0
33	8345.0
34	12994.0
35	16098.0
36	17352.0
37	27202.0
38	34958.0
39	37874.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72136324938896	19.636393595693846	23.690989481778484	14.951253673138714
2	28.466756378216573	26.40375690000824	27.118886112102818	18.01060060967237
3	32.10776371076264	24.616483124158954	27.04583527860929	16.229917886469117
4	29.324142476588033	26.107708785323926	25.65786944223217	18.910279295855876
5	25.739708345920416	30.815368137753	24.017795842144288	19.427127674182298
6	26.954659050339163	35.3950512179716	23.297174086178014	14.353115645511219
7	60.90187570373219	28.727652212122045	5.957213083239502	4.41325900090627
8	82.73418833932936	6.044544530799439	6.9771784802131105	4.244088649658089
9	76.09974459670997	8.88199269491665	9.245599099222805	5.772663609150578
10	43.779968692499935	26.597094444291873	15.802597973251311	13.820338889956885
11	33.811001565375	25.805069617993574	22.694092769065993	17.68983604756543
12	31.82434844698322	22.776480927141403	25.981929530662125	19.417241095213246
13	25.40136764342405	25.896795100650866	27.274325103671764	21.427512152253318
14	19.758877324032625	29.03468541454975	27.9740751929256	23.232362068492023
15	20.185647982863266	26.386180759618817	33.39192046796474	20.036250789553183
16	22.841842199214565	24.296817070826354	32.52245077307555	20.33888995688353
17	22.712218163842586	25.239337599209072	27.258396726443852	24.79004751050449
18	23.14997391041661	26.55315409331832	28.08941861423118	22.20745338203389
19	26.5624914179002	27.405047647818087	24.748304177079614	21.284156757202098
20	28.289347211160848	25.980281767500617	25.692472468623844	20.03789855271469
21	26.10221624145223	27.004641199571584	25.323373520445998	21.569769038530197
22	24.27319913217807	26.539422733639086	24.62142641364348	24.565951720539367
23	24.729080273528684	27.51544777963914	25.676544091395932	22.078927855436245
24	24.906489440584405	24.236399088237718	27.76975256089858	23.087358910279296
25	23.995825666657513	25.99456238156702	26.28731496992832	23.722296981847144
26	22.74517342707275	28.345371158652128	26.057726636091505	22.851728778183617
27	22.674319611127896	28.036690193062917	27.238623568505755	22.050366627303436
28	20.89638315986049	28.204762035536756	28.66503721198473	22.23381759261802
29	22.711119655068245	26.520198830088155	27.33803861258342	23.430642902260182
30	23.249388954494275	26.58501084777415	26.37464641748826	23.79095378024332
31	23.965616675363194	27.549501551643647	24.63790404525856	23.8469777277346
32	24.52475764150166	27.596188174553042	25.500782687501715	22.378271496443578
33	25.133880756872546	27.62529865707302	25.27998242385961	21.960838162194822
34	22.681459918161096	27.62914343778321	26.736055804245733	22.953340839809957
35	22.680910663773926	25.624914179002005	26.89533957652487	24.7988355806992
36	22.46340592645484	28.40084585175624	25.78035317057095	23.35539505121797
37	22.972015488973717	29.27031554664543	26.297750803284544	21.45991816109631
38	22.663334523384506	27.74119133276577	25.58426935435147	24.011204789498255
39	24.108422816027243	26.446598742207456	25.842968170708264	23.60201027105704
40	23.81237470134293	25.629857468486527	28.11688133358965	22.44088649658089
41	19.96924175431851	27.50665970944443	27.984511026281822	24.539587509955236
42	21.908658995413727	26.87007387471507	26.57567352319227	24.645593606678933
43	23.03847526982122	25.83637711806223	26.933238129239555	24.191909482876994
44	22.91269601515942	26.378491198198446	26.68222887430313	24.026583912339
45	21.91415153928542	26.698706505918217	25.89459808310219	25.492543871694174
46	21.670831845769367	28.399198088594733	25.56889023151073	24.361079834125174
47	21.509351055941558	26.53502869854173	28.482135501057314	23.473484744459398
48	22.183286188998437	24.27429764095241	27.96144234202071	25.58097382802845
49	20.648120176859912	26.140664048554086	28.12731716694587	25.083898607640126
50	19.722077280092275	26.667948260236727	28.05426633345234	25.555708126218658
51	20.465218465932498	25.78639496882981	26.668497514623894	27.079889050613794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	203.5
2	343.0
3	482.5
4	622.0
5	472.0
6	322.0
7	292.5
8	263.0
9	263.0
10	263.0
11	255.0
12	247.0
13	275.0
14	303.0
15	328.5
16	354.0
17	348.5
18	343.0
19	420.5
20	498.0
21	528.5
22	559.0
23	690.5
24	822.0
25	963.5
26	1253.5
27	1402.0
28	1766.0
29	2130.0
30	2686.5
31	3243.0
32	3752.0
33	4261.0
34	4808.5
35	5356.0
36	5887.5
37	6419.0
38	6853.0
39	7287.0
40	8074.5
41	8862.0
42	9749.5
43	10637.0
44	11214.5
45	11792.0
46	12969.5
47	14147.0
48	14661.0
49	15175.0
50	14669.0
51	14163.0
52	13056.0
53	11949.0
54	11006.5
55	10064.0
56	9748.0
57	9432.0
58	9435.0
59	9438.0
60	9336.5
61	9235.0
62	8361.5
63	7488.0
64	6143.0
65	4798.0
66	3906.5
67	3015.0
68	2548.0
69	2081.0
70	1718.0
71	1355.0
72	1136.5
73	918.0
74	729.0
75	420.0
76	300.0
77	257.0
78	214.0
79	171.5
80	129.0
81	93.0
82	57.0
83	46.5
84	36.0
85	25.0
86	14.0
87	10.5
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	182065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.200368000439404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.37417384566298	33.43860709087414
2	10.565659328137107	10.185373355669679
3	5.8594284151511005	8.472798176475434
4	3.802588996763754	7.331447559937384
5	2.7257395505720408	6.569082470546234
6	1.9360499566981173	5.599099222805042
7	1.5189844569032318	5.125092686677835
8	1.1588951182825107	4.468733694010381
9	0.8364100460367383	3.6283744816411723
>10	2.1913031587583753	13.4644220470711
>50	0.013674278681799537	0.4619229396094801
>100	0.017092848352249418	1.255046274682119
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	323	0.17740916705572185	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.1340180704693379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.492543871694175E-4	0.0	0.0	0.08293741246258204	0.0
2	5.492543871694175E-4	0.0	0.0	0.42182736934611265	0.0
3	5.492543871694175E-4	0.0	0.0	0.569576799494686	0.0
4	5.492543871694175E-4	0.0	0.0	0.7574217999066267	0.0
5	5.492543871694175E-4	0.0	0.0	1.3610523714058167	0.0
6	5.492543871694175E-4	0.0	0.0	1.8476917584379204	0.0
7	5.492543871694175E-4	0.0	0.0	2.287644522560624	0.0
8	5.492543871694175E-4	0.0	0.0	3.1428336033834072	0.0
9	5.492543871694175E-4	0.0	0.0	3.5168758410457803	0.0
10	5.492543871694175E-4	0.0	0.0	4.284184219921457	0.0
11	5.492543871694175E-4	0.0	0.0	5.089940405898992	0.0
12	5.492543871694175E-4	0.0	0.0	5.804520363606405	0.0
13	5.492543871694175E-4	0.0	0.0	6.094526680031857	0.0
14	5.492543871694175E-4	0.0	0.0	6.225249224178178	0.0
15	5.492543871694175E-4	0.0	0.0	6.431768873753879	0.0
16	5.492543871694175E-4	0.0	0.0	6.863482822069042	0.0
17	5.492543871694175E-4	0.0	0.0	7.363304314393211	0.0
18	5.492543871694175E-4	0.0	0.0	7.91200944717546	0.0
19	5.492543871694175E-4	0.0	0.0	8.252547167220499	0.0
20	5.492543871694175E-4	0.0	0.0	8.605717738170434	0.0
21	5.492543871694175E-4	0.0	0.0	9.039628704034273	0.0
22	5.492543871694175E-4	0.0	0.0	9.49441133661055	0.0
23	5.492543871694175E-4	0.0	0.0	9.99478208332189	0.0
24	5.492543871694175E-4	0.0	0.0	10.38310493505067	0.0
25	5.492543871694175E-4	0.0	0.0	10.714305330513827	0.0
26	5.492543871694175E-4	0.0	0.0	11.048251997912834	0.0
27	5.492543871694175E-4	0.0	0.0	11.38055090215033	0.0
28	5.492543871694175E-4	0.0	0.0	11.719440859033861	0.0
29	5.492543871694175E-4	0.0	0.0	12.04954274572268	0.0
30	5.492543871694175E-4	0.0	0.0	12.402713316672617	0.0
31	5.492543871694175E-4	0.0	0.0	12.779501826270838	0.0
32	5.492543871694175E-4	0.0	0.0	13.166176914838108	0.0
33	5.492543871694175E-4	0.0	0.0	13.509460906818994	0.0
34	5.492543871694175E-4	0.0	0.0	13.826380688215748	0.0
35	5.492543871694175E-4	0.0	0.0	14.193282618844918	0.0
36	5.492543871694175E-4	0.0	0.0	14.579957707412188	0.0
37	5.492543871694175E-4	0.0	0.0	14.959492488946255	0.0
38	5.492543871694175E-4	0.0	0.0	15.335731744157307	0.001098508774338835
39	5.492543871694175E-4	0.0	0.0	15.684508280009887	0.001098508774338835
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTA	30	2.1554115E-6	45.000004	1
CCTACTC	20	7.017635E-4	45.0	32
CGACAAT	20	7.017635E-4	45.0	20
ATATACT	55	1.8189894E-12	45.0	17
ACCACTA	25	3.877838E-5	45.0	22
TCATCGA	20	7.017635E-4	45.0	16
CCGGTAT	20	7.017635E-4	45.0	27
TTCATCG	25	3.877838E-5	45.0	15
TATACTG	55	1.8189894E-12	45.0	18
TCGACAA	20	7.017635E-4	45.0	19
GAATATA	60	3.6379788E-12	41.250004	15
CGAATAT	60	3.6379788E-12	41.250004	14
TAGGGCG	50	1.0713848E-9	40.5	5
CGTTAGG	35	6.2193667E-6	38.571426	2
CGTTTTT	165	0.0	38.181816	1
TACGAAT	65	9.094947E-12	38.07692	12
AGCTACG	65	9.094947E-12	38.07692	9
GGGTCTC	30	1.1361517E-4	37.500004	7
CGTTGAT	30	1.1361517E-4	37.500004	25
ATCGTTG	30	1.1361517E-4	37.500004	23
>>END_MODULE