##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936515.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200641
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.007660448263316	33.0	31.0	34.0	30.0	34.0
2	32.124236820988735	33.0	31.0	34.0	30.0	34.0
3	32.037918471299484	33.0	31.0	34.0	30.0	34.0
4	35.784570451702294	37.0	35.0	37.0	35.0	37.0
5	35.8487796611859	37.0	35.0	37.0	35.0	37.0
6	35.798122018929334	37.0	35.0	37.0	35.0	37.0
7	26.809729815939914	35.0	10.0	37.0	0.0	37.0
8	31.11997049456492	35.0	19.0	37.0	17.0	37.0
9	35.89954196799258	39.0	32.0	39.0	32.0	39.0
10	36.66095164996187	38.0	35.0	39.0	33.0	39.0
11	37.00435105486915	39.0	37.0	39.0	34.0	39.0
12	37.01553022562687	39.0	37.0	39.0	33.0	39.0
13	36.95319002596678	39.0	37.0	39.0	33.0	39.0
14	38.027860706435874	40.0	37.0	41.0	33.0	41.0
15	38.10940934305551	40.0	37.0	41.0	33.0	41.0
16	38.09528959684212	40.0	37.0	41.0	33.0	41.0
17	37.981618911389	40.0	37.0	41.0	33.0	41.0
18	37.89806669623856	40.0	36.0	41.0	33.0	41.0
19	37.782073454578075	40.0	36.0	41.0	33.0	41.0
20	37.85824432693218	40.0	36.0	41.0	33.0	41.0
21	37.78598591514197	40.0	36.0	41.0	33.0	41.0
22	37.808942339800936	40.0	36.0	41.0	33.0	41.0
23	37.74026744284568	40.0	36.0	41.0	33.0	41.0
24	37.67797209942135	40.0	36.0	41.0	33.0	41.0
25	37.567660647624365	39.0	36.0	41.0	33.0	41.0
26	37.437816797165084	39.0	35.0	41.0	33.0	41.0
27	37.31085371384712	39.0	35.0	41.0	32.0	41.0
28	37.266007446135134	39.0	35.0	41.0	32.0	41.0
29	37.239213321305215	39.0	35.0	41.0	32.0	41.0
30	37.13149356313017	39.0	35.0	41.0	32.0	41.0
31	36.96177750310255	39.0	35.0	41.0	31.0	41.0
32	36.83268624059888	39.0	35.0	41.0	31.0	41.0
33	36.71407638518548	39.0	35.0	41.0	31.0	41.0
34	36.564047228632234	39.0	35.0	41.0	30.0	41.0
35	36.47814255311726	39.0	35.0	41.0	30.0	41.0
36	36.28588872663115	39.0	35.0	41.0	30.0	41.0
37	36.239896132894074	39.0	35.0	40.0	30.0	41.0
38	35.98242632363276	39.0	35.0	40.0	29.0	41.0
39	35.903105546722756	38.0	35.0	40.0	29.0	41.0
40	35.67987599742824	38.0	35.0	40.0	27.0	41.0
41	35.595336945090985	38.0	35.0	40.0	27.0	41.0
42	35.5292138695481	38.0	35.0	40.0	27.0	41.0
43	35.49833284323742	38.0	34.0	40.0	27.0	41.0
44	35.307270199012166	38.0	34.0	40.0	26.0	41.0
45	35.1808154863662	38.0	34.0	40.0	26.0	41.0
46	35.049615980781596	38.0	34.0	40.0	26.0	41.0
47	34.87083896112958	37.0	34.0	40.0	25.0	41.0
48	34.79823665153184	37.0	34.0	40.0	24.0	41.0
49	34.6149540721986	37.0	34.0	40.0	24.0	41.0
50	34.40368618577459	37.0	33.0	40.0	24.0	41.0
51	32.17938008682174	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	15.0
11	13.0
12	7.0
13	12.0
14	13.0
15	24.0
16	40.0
17	64.0
18	111.0
19	185.0
20	289.0
21	475.0
22	637.0
23	920.0
24	1278.0
25	1707.0
26	2184.0
27	2694.0
28	3082.0
29	3594.0
30	4442.0
31	5257.0
32	6928.0
33	9484.0
34	14823.0
35	17684.0
36	19831.0
37	30176.0
38	36948.0
39	37695.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2855647649284	17.83583614515478	22.03288460484148	15.845714485075332
2	30.165320148922703	25.713587950618265	25.602444166446535	18.51864773401249
3	31.97302645022702	25.51671891587462	26.092373941517437	16.41788069238092
4	28.749358306627258	25.085102247297414	26.60572863970973	19.5598108063656
5	26.25684680598681	29.067837580554322	24.061881669250056	20.61343394420881
6	26.180591205187376	34.467531561345886	24.998878594105893	14.352998639360848
7	60.861439087723845	28.046610612985383	6.9148379443882355	4.177112354902538
8	81.43101360140749	5.999770734794982	8.05119591708574	4.518019746711788
9	75.37193295487961	7.065355535508695	10.382224968974436	7.180486540637257
10	42.42104056498921	25.49130038227481	17.011478212329482	15.076180840406497
11	32.9145089986593	25.537651825897996	23.624782571857196	17.923056603585508
12	27.993281532687735	24.253268275178055	27.799402913661712	19.954047278472494
13	25.00236741244312	25.495786005851244	30.517690800982848	18.984155780722784
14	21.383465991497253	27.688259129489985	29.726725843671037	21.20154903534173
15	20.61094193111079	24.589191640791263	34.18045165245388	20.61941477564406
16	24.830916911299287	25.189766797414286	29.212872742859137	20.76644354842729
17	25.491798784894414	24.390328995569202	27.371275063421734	22.746597156114653
18	26.88184369097044	23.08451413220628	28.819134673371842	21.214507503451436
19	25.6697285200931	25.800310006429395	26.137728579901413	22.39223289357609
20	27.757038690995362	26.453715840730457	26.188067244481438	19.601178223792743
21	25.678699767245977	26.37197781111538	27.67231024566265	20.277012175975997
22	25.73452086064164	23.355146754651344	27.613000333929755	23.297332050777257
23	23.481242617411198	27.028374061134063	27.477933224017026	22.01245009743771
24	23.735926356028926	24.638533500132077	28.665128263914152	22.96041187992484
25	23.35115953369451	26.764220672743853	27.385728739390252	22.49889105417138
26	23.331721831529947	25.757447381143432	27.028374061134063	23.88245672619255
27	22.739619519440193	26.275287702912166	26.984514630608896	24.00057814703874
28	21.08193240663673	26.393409123758353	28.957192199002197	23.56746627060272
29	22.619006085495986	26.03555604288256	26.819044960900314	24.52639291072114
30	23.275402335514674	25.102546338983554	28.485204918237052	23.136846407264716
31	25.018316296270456	24.542840197168076	27.30548591763398	23.133357588927485
32	25.403581521224478	25.25256552748441	25.955811623745895	23.388041327545217
33	23.539555723904883	26.17760078946975	25.97225891019283	24.310584576432532
34	22.261153004620194	25.164348263814475	29.494968625555096	23.07953010601024
35	22.49639904107336	26.402380370911228	27.17041880772125	23.930801780294157
36	23.93478900125099	28.410444525296423	25.93637392158133	21.71839255187125
37	22.744603545636235	26.852936339033395	27.251160032097125	23.151300083233238
38	23.24848859405605	28.52806754352301	25.147900977367538	23.075542885053405
39	22.88714669484303	25.911453790601126	26.970060954640378	24.23133855991547
40	25.2630319824961	24.881255575879308	27.228233511595334	22.62747893002926
41	21.533485179998106	26.371479408495773	27.119083337902023	24.9759520736041
42	23.947249066741094	24.52639291072114	27.715671273568216	23.81068674896955
43	23.43887839474484	24.700833827582596	27.50235495237763	24.357932825294927
44	23.11591349724134	25.33928758329554	27.78046361411676	23.764335305346364
45	22.880667460788175	24.250776262080034	26.96557533106394	25.902980946067856
46	23.45532568119178	26.16364551612083	26.215977791179267	24.165051011508115
47	21.437293474414503	25.887032062240518	29.326508540128888	23.34916592321609
48	22.42612427170917	26.071441031494064	27.410648870370462	24.091785826426303
49	23.361127586086592	25.03326837485858	28.708489291819717	22.89711474723511
50	22.144526791632817	25.450431367467267	28.121371005926004	24.283670834973908
51	22.289561953937632	25.24359428033154	26.10333879914873	26.363504966582102
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	209.5
2	366.0
3	577.5
4	789.0
5	593.5
6	398.0
7	384.0
8	370.0
9	347.0
10	324.0
11	345.0
12	366.0
13	412.5
14	459.0
15	444.5
16	430.0
17	423.0
18	416.0
19	516.0
20	616.0
21	660.0
22	704.0
23	810.0
24	916.0
25	1041.0
26	1339.5
27	1513.0
28	1854.5
29	2196.0
30	2566.0
31	2936.0
32	3462.5
33	3989.0
34	4357.0
35	4725.0
36	5318.0
37	5911.0
38	6757.0
39	7603.0
40	8510.5
41	9418.0
42	10133.0
43	10848.0
44	11989.0
45	13130.0
46	15253.0
47	17376.0
48	17038.5
49	16701.0
50	16666.5
51	16632.0
52	15670.5
53	14709.0
54	13720.5
55	12732.0
56	11932.5
57	11133.0
58	10424.5
59	9716.0
60	9049.5
61	8383.0
62	7472.0
63	6561.0
64	5830.5
65	5100.0
66	4490.0
67	3880.0
68	3255.5
69	2631.0
70	2272.5
71	1914.0
72	1638.5
73	1363.0
74	1154.0
75	773.5
76	602.0
77	443.0
78	284.0
79	211.0
80	138.0
81	114.5
82	91.0
83	72.0
84	53.0
85	39.5
86	26.0
87	19.0
88	12.0
89	8.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	3.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.51571214258302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52710413694722	32.56114154135994
2	10.912981455064195	10.370761708723542
3	6.020810606696316	8.582493109583785
4	4.037299655953679	7.67340673142578
5	2.6757992783418647	6.357125413051171
6	2.010782915163212	5.732626930687148
7	1.5209364773013343	5.058786588982311
8	1.1475203490811445	4.362019726775683
9	0.8548711924141982	3.655783214796577
>10	2.2677687337417134	13.694110376244137
>50	0.011538138793320466	0.3488818337229181
>100	0.010489217084836789	0.9419809510518786
>500	0.0020978434169673576	0.6608818735951276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTC	685	0.34140579442885555	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCG	641	0.31947607916627213	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGC	421	0.20982750285335497	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.1574952277949173	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCATGATCGTATGCCGTCTTCTGC	231	0.11513100512856296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07326518508181279	0.0
2	0.0	0.0	0.0	0.4301214607183975	0.0
3	0.0	0.0	0.0	0.5457508684665646	0.0
4	0.0	0.0	0.0	0.7909649573118156	0.0
5	0.0	0.0	0.0	1.6561919049446523	0.0
6	0.0	0.0	0.0	2.0997702363923625	0.0
7	0.0	0.0	0.0	2.532882112828385	0.0
8	0.0	0.0	0.0	3.359732058751701	0.0
9	0.0	0.0	0.0	3.659272033133806	0.0
10	0.0	0.0	0.0	4.654582064483331	0.0
11	0.0	0.0	0.0	5.335400042862625	0.0
12	0.0	0.0	0.0	6.190658938103379	0.0
13	0.0	0.0	0.0	6.453317118634776	0.0
14	0.0	0.0	0.0	6.583400202351464	0.0
15	0.0	0.0	0.0	6.799706939259673	0.0
16	0.0	0.0	0.0	7.185968969452904	0.0
17	0.0	0.0	0.0	7.586186272995051	0.0
18	0.0	0.0	0.0	8.073125632348324	0.0
19	0.0	0.0	0.0	8.349739086228638	0.0
20	0.0	0.0	0.0	8.621368513912909	0.0
21	0.0	0.0	0.0	8.99816089433366	0.0
22	0.0	0.0	0.0	9.368474040699558	0.0
23	0.0	0.0	0.0	9.733803160869414	0.0
24	0.0	0.0	0.0	10.04430799288281	0.0
25	0.0	0.0	0.0	10.328397486057186	0.0
26	0.0	0.0	0.0	10.595541290165022	0.0
27	0.0	0.0	0.0	10.892589251449106	0.0
28	0.0	0.0	0.0	11.157241042458919	0.0
29	0.0	0.0	0.0	11.445317756590129	0.0
30	0.0	0.0	0.0	11.831081384163754	0.0
31	0.0	0.0	0.0	12.112678864240111	0.0
32	0.0	0.0	0.0	12.41271724124182	0.0
33	0.0	0.0	0.0	12.702289163231843	0.0
34	0.0	0.0	0.0	13.004819553331572	0.0
35	0.0	0.0	0.0	13.322302022019429	0.0
36	4.984026196041686E-4	0.0	0.0	13.619349983303513	0.0
37	4.984026196041686E-4	0.0	0.0	13.892474618846597	0.0
38	9.968052392083373E-4	0.0	0.0	14.227899581840202	0.0
39	9.968052392083373E-4	0.0	0.0	14.736768656456059	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATGC	20	7.019354E-4	45.0	11
GCGCCAA	20	7.019354E-4	45.0	1
TCGATCA	20	7.019354E-4	45.0	17
CTAACGG	25	3.8792616E-5	45.0	2
GACGGGA	40	6.7666406E-9	45.0	4
GCGATAT	25	3.8792616E-5	45.0	9
TCGTAGG	20	7.019354E-4	45.0	2
TAGGCGA	20	7.019354E-4	45.0	6
CGATCAG	20	7.019354E-4	45.0	18
AATACGG	20	7.019354E-4	45.0	2
TAACGGG	45	1.9146682E-8	40.0	3
TGGGCGA	130	0.0	39.807693	6
CACGAGC	40	3.4392178E-7	39.375	41
ATGCGGG	40	3.4392178E-7	39.375	3
AGGCGAT	75	0.0	39.0	7
GGCGATA	75	0.0	39.0	8
ACACGGG	35	6.222537E-6	38.57143	3
CACGTGA	100	0.0	38.25	43
TGATACC	95	0.0	37.894737	4
ACACGAC	115	0.0	37.173916	26
>>END_MODULE