##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936514.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69857
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85623487982593	31.0	31.0	34.0	30.0	34.0
2	31.97240076155575	33.0	31.0	34.0	30.0	34.0
3	31.882975220808223	33.0	31.0	34.0	30.0	34.0
4	35.66435718682451	37.0	35.0	37.0	33.0	37.0
5	35.73039208669138	37.0	35.0	37.0	35.0	37.0
6	35.70240634438925	37.0	35.0	37.0	35.0	37.0
7	26.851883132685344	35.0	11.0	37.0	0.0	37.0
8	31.088022674892997	35.0	19.0	37.0	17.0	37.0
9	35.82477060280287	39.0	32.0	39.0	32.0	39.0
10	36.613968535722975	37.0	35.0	39.0	32.0	39.0
11	36.942410925175714	39.0	37.0	39.0	33.0	39.0
12	36.82512847674535	39.0	35.0	39.0	33.0	39.0
13	36.561432640966544	39.0	35.0	39.0	33.0	39.0
14	37.72071517528666	40.0	36.0	41.0	33.0	41.0
15	37.77041670841863	40.0	36.0	41.0	33.0	41.0
16	37.77664371501782	40.0	36.0	41.0	33.0	41.0
17	37.68726111914339	39.0	36.0	41.0	33.0	41.0
18	37.58143063687247	39.0	36.0	41.0	33.0	41.0
19	37.45962466180912	39.0	36.0	41.0	32.0	41.0
20	37.534434630745665	39.0	35.0	41.0	33.0	41.0
21	37.49114619866298	39.0	35.0	41.0	33.0	41.0
22	37.46646721158939	39.0	35.0	41.0	33.0	41.0
23	37.488870120388796	39.0	35.0	41.0	33.0	41.0
24	37.44025652404197	39.0	35.0	41.0	33.0	41.0
25	37.37586784431052	39.0	35.0	41.0	33.0	41.0
26	37.203358289076256	39.0	35.0	41.0	32.0	41.0
27	37.13237041384543	39.0	35.0	41.0	32.0	41.0
28	37.12435403753382	39.0	35.0	41.0	32.0	41.0
29	37.11771189716134	39.0	35.0	41.0	32.0	41.0
30	37.063143278411616	39.0	35.0	41.0	32.0	41.0
31	36.924417023347694	39.0	35.0	41.0	32.0	41.0
32	36.8015374264569	39.0	35.0	40.0	31.0	41.0
33	36.712627224186555	39.0	35.0	40.0	31.0	41.0
34	36.56425268763331	39.0	35.0	40.0	31.0	41.0
35	36.54073321213336	39.0	35.0	40.0	31.0	41.0
36	36.38080650471678	39.0	35.0	40.0	30.0	41.0
37	36.30754255121176	39.0	35.0	40.0	30.0	41.0
38	36.15909643987002	38.0	35.0	40.0	30.0	41.0
39	36.13723749946319	38.0	35.0	40.0	30.0	41.0
40	35.853114219047484	38.0	35.0	40.0	29.0	41.0
41	35.88860099918405	38.0	35.0	40.0	29.0	41.0
42	35.831770617117826	38.0	35.0	40.0	29.0	41.0
43	35.79934723792891	38.0	35.0	40.0	29.0	41.0
44	35.51676997294473	38.0	35.0	40.0	28.0	41.0
45	35.4089497115536	38.0	34.0	40.0	28.0	41.0
46	35.21206178335743	38.0	34.0	40.0	27.0	41.0
47	35.0490001002047	37.0	34.0	40.0	26.0	41.0
48	34.96416966087865	37.0	34.0	40.0	26.0	41.0
49	34.796541506219846	37.0	34.0	40.0	25.0	41.0
50	34.49817484289334	37.0	34.0	40.0	24.0	41.0
51	32.02704095509398	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	7.0
11	4.0
12	1.0
13	1.0
14	2.0
15	4.0
16	4.0
17	10.0
18	27.0
19	53.0
20	75.0
21	103.0
22	183.0
23	301.0
24	375.0
25	501.0
26	735.0
27	861.0
28	992.0
29	1288.0
30	1558.0
31	1919.0
32	2607.0
33	3639.0
34	6044.0
35	7747.0
36	6482.0
37	9934.0
38	12253.0
39	12137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60192965629787	17.952388450692126	22.236855290092617	16.20882660291739
2	27.172652704811256	29.05793263380907	26.172037161630186	17.597377499749488
3	28.873269679488097	28.612737449360836	26.726026024593097	15.78796684655797
4	27.08103697553574	24.29964069456175	30.1902457878237	18.429076542078818
5	28.16324777760282	27.620710880799347	24.150765134489028	20.06527620710881
6	24.45281074194426	35.58125885738008	24.8507665659848	15.115163834690867
7	58.57394391399573	27.56201955423222	9.5767067008317	4.287329830940349
8	76.84985040869205	8.38999670755973	9.390612250740798	5.369540633007429
9	71.71364358618321	7.604105529868159	10.306769543496	10.37548134045264
10	42.94201010636014	25.32888615314142	17.59594600397956	14.13315773651889
11	35.89905091830454	23.333381049858996	23.708432941580657	17.059135090255808
12	33.6258356356557	21.40229325622343	27.03236611935812	17.93950498876276
13	22.26405370972129	29.742187611835607	30.63973545958172	17.35402321886139
14	18.09696952345506	34.33728903331091	26.339522166711998	21.226219276522038
15	16.575289520019467	24.86937601099389	40.99517585925533	17.560158609731307
16	18.028257726498417	25.39044047124841	34.544855919950756	22.03644588230242
17	18.70535522567531	22.55035286370729	30.918877134718066	27.825414775899336
18	21.947693144566756	24.727657929770817	31.30538099259917	22.019267933063258
19	23.695549479651287	26.92500393661337	26.126229296992427	23.253217286742917
20	27.633594342728717	24.99677913451766	28.244843036488827	19.1247834862648
21	22.902500823110067	29.112329473066406	27.52193767267418	20.463232031149346
22	20.818242982091988	25.195757046537924	26.243611950126688	27.742388021243396
23	19.82335342199064	28.979200366462916	27.77388092818186	23.423565283364585
24	22.171006484675836	23.21599839672474	30.900267689708976	23.71272742889045
25	18.748300098773207	28.82889331062027	29.54034670827548	22.882459882331048
26	19.091859083556407	28.45527291466854	28.79310591637202	23.659762085403038
27	21.47529954048986	28.817441344460825	26.884922055055327	22.822337059993988
28	16.17160771289921	27.609258914639906	34.164078045149374	22.055055327311507
29	21.12458307685701	26.363857594800805	29.704968721817426	22.806590606524757
30	19.564252687633306	28.59699099589161	29.128075926535637	22.710680389939448
31	24.938087807950527	26.342385158251858	27.16406373019168	21.555463303605936
32	27.66795024120704	28.11314542565527	24.435632792705096	19.783271540432597
33	22.808022102294686	27.29003535794552	24.298209198791817	25.603733340967977
34	21.382252315444408	27.56488254577208	30.356299297135575	20.69656584164794
35	22.52744893138841	25.530727056701547	25.950155317291035	25.99166869461901
36	22.849535479622656	27.278583391786075	27.501896731895158	22.369984396696108
37	22.414360765563938	26.256495412056058	30.072863134689438	21.25628068769057
38	20.59779263352277	29.39862862705241	25.99453168615887	24.00904705326596
39	24.439927280014885	25.424796369726728	28.383698126172035	21.751578224086348
40	25.301687733512747	24.689007543982708	28.33932175730421	21.669982965200337
41	19.13480395665431	25.30025623774282	26.638704782627364	28.926235022975504
42	23.45792118184291	23.755672301988348	28.030118670999325	24.756287845169417
43	24.508639076971527	23.45792118184291	24.928067337561018	27.105372403624546
44	21.257712183460498	26.230728488197318	28.89903660334684	23.612522724995348
45	20.74094221051577	26.723163033053236	25.8098687318379	26.726026024593097
46	23.42070229182473	28.385129621941967	25.888600999184046	22.30556708704926
47	19.884907740097628	24.98389567258829	32.391886281976035	22.739310305338048
48	22.67775598723106	23.709864437350586	28.00435174714059	25.60802782827777
49	20.614970582761927	22.31845054897863	32.301702048470446	24.764876819789
50	19.738895171564767	25.381851496628826	29.68349628526848	25.195757046537924
51	19.733169188485046	25.250153885795267	25.95588130037076	29.060795625348927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	78.5
2	117.0
3	234.0
4	351.0
5	267.0
6	183.0
7	162.5
8	142.0
9	146.5
10	151.0
11	139.5
12	128.0
13	135.5
14	143.0
15	149.0
16	155.0
17	145.0
18	135.0
19	162.0
20	189.0
21	207.0
22	225.0
23	219.0
24	213.0
25	341.5
26	507.0
27	544.0
28	619.0
29	694.0
30	809.0
31	924.0
32	1166.0
33	1408.0
34	1479.0
35	1550.0
36	1672.0
37	1794.0
38	2181.0
39	2568.0
40	3067.5
41	3567.0
42	4179.0
43	4791.0
44	5329.5
45	5868.0
46	7684.0
47	9500.0
48	8242.0
49	6984.0
50	6670.5
51	6357.0
52	5573.0
53	4789.0
54	4069.0
55	3349.0
56	3021.0
57	2693.0
58	2581.0
59	2469.0
60	2537.0
61	2605.0
62	2163.5
63	1722.0
64	1409.5
65	1097.0
66	900.5
67	704.0
68	582.5
69	461.0
70	401.0
71	341.0
72	270.5
73	200.0
74	162.5
75	94.5
76	64.0
77	42.5
78	21.0
79	17.5
80	14.0
81	8.5
82	3.0
83	2.0
84	1.0
85	2.5
86	4.0
87	2.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	69857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.57269851267589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.07045913947444	35.72727142591294
2	10.231013572047358	10.143579025723978
3	5.339301183944556	7.940507035801709
4	3.632688420444701	7.20328671428776
5	2.4516315333525847	6.0766995433528495
6	1.6228703436326886	4.8270037362039595
7	1.2676869766098757	4.39898650099489
8	0.9846953508518625	3.9051204603690395
9	0.6814900375397054	3.0404970153313196
>10	1.663297718740976	10.896545800707159
>50	0.020213687554143805	0.7730077157621998
>100	0.02598902685532775	2.0885523283278697
>500	0.008663008951775918	2.97894269722433
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	786	1.1251556751649798	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	666	0.95337618277338	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	629	0.90041083928597	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	260	0.37218890018179995	Illumina Single End Adapter 1 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	199	0.28486765821607	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	185	0.26482671743704994	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	174	0.24908026396781996	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.21186137394964	No Hit
GAATCTATCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	133	0.19038893740069	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	133	0.19038893740069	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC	126	0.18036846701117998	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	101	0.14458107276292997	No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	100	0.143149576993	No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	86	0.12310863621397998	Illumina Single End Adapter 1 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTT	81	0.11595115736433	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	78	0.11165667005454	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19897791202027	0.0
2	0.0	0.0	0.0	1.2597162775383999	0.0
3	0.0	0.0	0.0	1.57607684269293	0.0
4	0.0	0.0	0.0	2.11718224372647	0.0
5	0.0	0.0	0.0	4.1799676481956	0.0
6	0.0	0.0	0.0	4.768312409636829	0.0
7	0.0	0.0	0.0	5.650113803913709	0.0
8	0.0	0.0	0.0	6.977110382638819	0.0
9	0.0	0.0	0.0	7.449503986715719	0.0
10	0.0	0.0	0.0	9.921697181384829	0.0
11	0.0	0.0	0.0	11.086934738107848	0.0
12	0.0	0.0	0.0	13.265671299941308	0.0
13	0.0	0.0	0.0	13.775283794036389	0.0
14	0.0	0.0	0.0	14.004323117225189	0.0
15	0.0	0.0	0.0	14.48816868746153	0.0
16	0.0	0.0	0.0	15.229683496285269	0.0
17	0.0	0.0	0.0	16.00984869089712	0.0
18	0.0	0.0	0.0	16.999012267918747	0.0
19	0.0	0.0	0.0	17.4499334354467	0.0
20	0.0	0.0	0.0	17.932347509913107	0.0
21	0.0	0.0	0.0	18.608013513320067	0.0
22	0.0	0.0	0.0	19.21783071131025	0.0
23	0.0	0.0	0.0	19.81046996006127	0.0
24	0.0	0.0	0.0	20.334397411855647	0.0
25	0.0	0.0	0.0	20.685113875488497	0.0
26	0.0	0.0	0.0	21.124583076857007	0.0
27	0.0	0.0	0.0	21.469573557410136	0.0
28	0.0	0.0	0.0	21.883275834919907	0.0
29	0.0	0.0	0.0	22.299841103969538	0.0
30	0.0	0.0	0.0	22.785118169975807	0.0
31	0.0	0.0	0.0	23.243196816353407	0.0
32	0.0	0.0	0.0	23.679803026182057	0.0
33	0.0	0.0	0.0	24.04769743905407	0.0
34	0.0	0.0	0.0	24.46855719541349	0.0
35	0.0	0.0	0.0	24.93379332064074	0.0
36	0.0	0.0	0.0	25.39902944586799	0.0
37	0.0	0.0	0.0	25.875717537254676	0.0
38	0.0	0.0	0.0	26.282262335914798	0.0
39	0.0	0.0	0.0	26.704553588044146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTAT	30	2.136254E-6	45.000004	2
TATACTG	30	2.136254E-6	45.000004	18
GGACTAA	30	2.136254E-6	45.000004	8
ATCAGGA	30	2.136254E-6	45.000004	16
GAATCTA	30	2.136254E-6	45.000004	1
TACGAAT	30	2.136254E-6	45.000004	12
CTACGAA	30	2.136254E-6	45.000004	11
ACGGCTG	115	0.0	45.000004	8
GACTATG	30	2.136254E-6	45.000004	24
ACTATGA	30	2.136254E-6	45.000004	25
GACTAAT	30	2.136254E-6	45.000004	9
ACACGAC	20	6.9878285E-4	45.0	26
CCTATGC	25	3.853177E-5	45.0	35
TCAAGCG	20	6.9878285E-4	45.0	17
ACATGGT	20	6.9878285E-4	45.0	11
AAACACG	20	6.9878285E-4	45.0	40
CACGTGA	20	6.9878285E-4	45.0	43
GTCAAGC	20	6.9878285E-4	45.0	16
AGCTACG	25	3.853177E-5	45.0	9
GATGCCA	25	3.853177E-5	45.0	9
>>END_MODULE