##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197756
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27263395295212	33.0	31.0	34.0	31.0	34.0
2	32.400695806954026	34.0	31.0	34.0	31.0	34.0
3	32.325284694269705	34.0	31.0	34.0	30.0	34.0
4	36.0136835291976	37.0	35.0	37.0	35.0	37.0
5	36.058430591233645	37.0	35.0	37.0	35.0	37.0
6	36.027908129209735	37.0	35.0	37.0	35.0	37.0
7	26.616466757013693	37.0	8.0	37.0	0.0	37.0
8	31.122929266368658	37.0	19.0	37.0	17.0	37.0
9	36.087567507433405	39.0	32.0	39.0	32.0	39.0
10	36.915466534517286	38.0	37.0	39.0	34.0	39.0
11	37.29275976455835	39.0	37.0	39.0	34.0	39.0
12	37.33679888347256	39.0	37.0	39.0	35.0	39.0
13	37.291075871275716	39.0	37.0	39.0	35.0	39.0
14	38.56002346325775	40.0	38.0	41.0	35.0	41.0
15	38.62323267056372	40.0	38.0	41.0	35.0	41.0
16	38.57258439693359	40.0	38.0	41.0	35.0	41.0
17	38.49501911446429	40.0	38.0	41.0	34.0	41.0
18	38.40487772810939	40.0	38.0	41.0	34.0	41.0
19	38.301118550132486	40.0	37.0	41.0	34.0	41.0
20	38.34537005198325	40.0	37.0	41.0	34.0	41.0
21	38.29305811201683	40.0	37.0	41.0	34.0	41.0
22	38.27126357733773	40.0	37.0	41.0	34.0	41.0
23	38.2117205040555	40.0	37.0	41.0	34.0	41.0
24	38.15610145836283	40.0	37.0	41.0	34.0	41.0
25	38.0438166225045	40.0	37.0	41.0	34.0	41.0
26	37.93861121786444	40.0	37.0	41.0	34.0	41.0
27	37.79435769331904	40.0	36.0	41.0	33.0	41.0
28	37.75116810615101	40.0	36.0	41.0	33.0	41.0
29	37.74104451950889	40.0	36.0	41.0	33.0	41.0
30	37.680697425109734	40.0	36.0	41.0	33.0	41.0
31	37.58283440199033	40.0	36.0	41.0	33.0	41.0
32	37.4579431218269	40.0	36.0	41.0	33.0	41.0
33	37.37566496086086	40.0	36.0	41.0	33.0	41.0
34	37.25219968041425	40.0	35.0	41.0	32.0	41.0
35	37.17900341835393	40.0	35.0	41.0	32.0	41.0
36	37.03477012075487	40.0	35.0	41.0	32.0	41.0
37	36.986847428143776	40.0	35.0	41.0	31.0	41.0
38	36.88000869758692	39.0	35.0	41.0	31.0	41.0
39	36.84051052812557	39.0	35.0	41.0	31.0	41.0
40	36.62359675559781	39.0	35.0	41.0	31.0	41.0
41	36.55978579663828	39.0	35.0	41.0	31.0	41.0
42	36.475970387750564	39.0	35.0	41.0	30.0	41.0
43	36.42889722688566	39.0	35.0	41.0	30.0	41.0
44	36.25972410445195	39.0	35.0	40.0	30.0	41.0
45	36.18375675074334	39.0	35.0	40.0	30.0	41.0
46	36.059057626570116	38.0	35.0	40.0	30.0	41.0
47	35.928942737514916	38.0	35.0	40.0	29.0	41.0
48	35.830953295980905	38.0	35.0	40.0	29.0	41.0
49	35.71410222698679	38.0	35.0	40.0	28.0	41.0
50	35.52254798843018	38.0	34.0	40.0	28.0	41.0
51	33.509719047715365	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	22.0
10	27.0
11	13.0
12	6.0
13	6.0
14	14.0
15	20.0
16	28.0
17	47.0
18	72.0
19	109.0
20	178.0
21	296.0
22	466.0
23	641.0
24	905.0
25	1249.0
26	1512.0
27	1765.0
28	2089.0
29	2473.0
30	3017.0
31	3889.0
32	5328.0
33	7875.0
34	13047.0
35	16999.0
36	18155.0
37	30231.0
38	40118.0
39	47141.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.406581848338355	18.661886365015473	24.368413600598718	15.563118186047454
2	29.685572119177166	25.835372883755742	26.511458565100426	17.967596431966665
3	33.00228564493619	24.353243390845282	26.27176925099618	16.372701713222355
4	30.333340075648778	25.858128198385888	25.137543235097798	18.670988490867536
5	26.243957199781548	30.637755618034348	23.873864762636785	19.244422419547323
6	27.285139262525536	36.06666801512976	23.25643722567204	13.391755496672667
7	61.318493497036755	29.362446651429035	5.4607698375776215	3.858290013956593
8	84.6512874451344	5.388458504419588	6.474645522765428	3.485608527680576
9	77.99712777362001	8.565100426788568	8.316308986832258	5.121462812759157
10	43.65430126013876	27.16226056352273	15.10952891441979	14.073909261918729
11	34.37266125932968	27.136471206941888	21.43095531867554	17.059912215052893
12	31.872610691963832	24.111025708448796	25.245251724347174	18.771111875240194
13	25.231598535569084	26.733449301159002	26.5630372782621	21.47191488500981
14	20.92932704949534	29.83575719573616	26.810817370901514	22.42409838386699
15	20.221889601326886	27.370092437144766	32.47486801917515	19.933149942353204
16	23.652379700236654	24.63035255567467	31.870082323671596	19.84718542041708
17	23.069337972046362	24.969659580493133	28.120512146281275	23.840490301179234
18	23.718117275834867	26.42144865389672	27.11270454499484	22.74772952527357
19	26.347114626104894	27.25227047472643	25.56332045551083	20.837294443657843
20	29.58999979773054	25.345375108719836	25.781771475960273	19.282853617589353
21	26.875543599182834	26.217156495883813	25.23817229312891	21.669127611804445
22	24.77800926394142	25.943081373005118	25.9471267622727	23.33178260078076
23	25.363579360423955	26.849248568943544	26.732943627500553	21.05422844313194
24	24.729464592730434	24.73300430833957	28.60949857400028	21.928032524929712
25	23.96438034749894	26.607030886547058	26.671757114828377	22.75683165112563
26	23.300430833956998	29.088877202208785	26.294524565626325	21.31616739820789
27	24.17221222112098	28.56297659742309	26.489208924128725	20.77560225732721
28	21.94219138736625	28.96144744027994	28.050223507757032	21.046137664596777
29	23.40004854467121	27.050506685005764	27.921276724852852	21.628168045470176
30	23.41825279637533	26.950888974291555	27.586520762960415	22.044337466372703
31	24.98381844292967	26.815368433827548	25.933979247153054	22.266833876089727
32	25.577479317947372	28.17006816480916	25.427294241388378	20.825158275855095
33	25.387346022371005	27.838346244867413	25.501122595521753	21.27318513723983
34	23.753008758267764	27.861101559497563	27.059608810857828	21.32628087137685
35	24.267784542567608	26.240923157830863	26.790590424563604	22.700701875037925
36	23.780315135823944	28.759683650559275	25.77418637108356	21.685814842533222
37	23.935051275308965	29.609215396751555	25.80503246424887	20.65070086369061
38	24.02860090212181	28.38396812233257	24.99544893707397	22.591982038471652
39	25.38987439066324	26.311211796355106	25.993143065191447	22.305770747790206
40	24.768401464430916	26.359250793907645	27.652258338558628	21.22008940310281
41	21.817289993729645	27.642144865389675	27.794352636582452	22.746212504298228
42	23.0263557110783	28.081069600922348	26.573656425089503	22.318918262909847
43	24.29256255183155	26.901332955763667	26.76884645725035	22.037258035154434
44	23.250874815429114	27.875766095592546	26.51095289144198	22.36240619753636
45	22.76795647161148	26.91245777624952	26.048261493962254	24.271324258176744
46	22.331560104371043	28.455773781832157	26.198952244179697	23.013713869617103
47	23.54871660025486	26.878577641133518	27.99915046825381	21.573555290357817
48	23.371730819798135	25.861162240336576	27.639616497097435	23.127490442767858
49	22.48730759117296	27.10360241914278	27.578429984425252	22.830660005259006
50	20.987985193875282	27.173385384008576	27.519266166386863	24.319363255729282
51	21.319707113817028	26.732943627500553	26.83053864358098	25.116810615101436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	246.5
2	427.0
3	655.0
4	883.0
5	659.0
6	435.0
7	377.5
8	320.0
9	326.5
10	333.0
11	364.0
12	395.0
13	412.5
14	430.0
15	465.5
16	501.0
17	506.0
18	511.0
19	565.0
20	619.0
21	647.0
22	675.0
23	789.0
24	903.0
25	1231.0
26	1813.5
27	2068.0
28	2663.0
29	3258.0
30	3801.0
31	4344.0
32	4675.5
33	5007.0
34	5414.0
35	5821.0
36	6170.0
37	6519.0
38	7416.5
39	8314.0
40	9087.5
41	9861.0
42	10437.5
43	11014.0
44	11791.5
45	12569.0
46	13321.0
47	14073.0
48	13943.0
49	13813.0
50	13514.5
51	13216.0
52	12584.0
53	11952.0
54	11375.0
55	10798.0
56	10685.5
57	10573.0
58	10493.5
59	10414.0
60	10540.0
61	10666.0
62	9595.0
63	8524.0
64	7226.5
65	5929.0
66	4898.5
67	3868.0
68	3214.0
69	2560.0
70	2096.0
71	1632.0
72	1406.0
73	1180.0
74	990.5
75	638.0
76	475.0
77	350.5
78	226.0
79	164.5
80	103.0
81	76.5
82	50.0
83	41.5
84	33.0
85	22.5
86	12.0
87	10.5
88	9.0
89	6.5
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.35016889500192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.03761194029851	26.696535124092314
2	11.771940298507463	9.970873197273407
3	6.906268656716417	8.774449321385951
4	4.765373134328358	8.072574283460426
5	3.4388059701492537	7.281700681648092
6	2.515820895522388	6.392726390096887
7	1.964179104477612	5.822832177026235
8	1.422089552238806	4.818058617690487
9	1.106865671641791	4.218835332429863
>10	3.0376119402985076	16.50771658002791
>50	0.020298507462686566	0.5683771920953093
>100	0.013134328358208954	0.8753211027731144
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	333	0.16838932826311212	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	221	0.1117538785169603	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02932907218997148	0.0
2	0.0	0.0	0.0	0.14563401363296183	0.0
3	0.0	0.0	0.0	0.2073261999635915	0.0
4	0.0	0.0	0.0	0.292785048241267	0.0
5	0.0	0.0	0.0	0.6037743481866542	0.0
6	0.0	0.0	0.0	0.8085721798580069	0.0
7	0.0	0.0	0.0	1.0017395173850603	0.0
8	0.0	0.0	0.0	1.40577277048484	0.0
9	0.0	0.0	0.0	1.5746677724063998	0.0
10	0.0	0.0	0.0	2.000444992819434	0.0
11	0.0	0.0	0.0	2.463642063957604	0.0
12	0.0	0.0	0.0	2.8964987155889075	0.0
13	0.0	0.0	0.0	3.056797265316855	0.0
14	0.0	0.0	0.0	3.1129270414045593	0.0
15	0.0	0.0	0.0	3.2332773721151318	0.0
16	0.0	0.0	0.0	3.466898602318008	0.0
17	0.0	0.0	0.0	3.7222637998341392	0.0
18	0.0	0.0	0.0	4.001395659297316	0.0
19	0.0	0.0	0.0	4.174841724144906	0.0
20	0.0	0.0	0.0	4.355872893869212	0.0
21	0.0	0.0	0.0	4.571289872367968	0.0
22	0.0	0.0	0.0	4.815024575739801	0.0
23	0.0	0.0	0.0	5.049657153259572	0.0
24	0.0	0.0	0.0	5.242824490786626	0.0
25	0.0	0.0	0.0	5.420821618560246	0.0
26	0.0	0.0	0.0	5.571006695119237	0.0
27	0.0	0.0	0.0	5.725237160945812	0.0
28	0.0	0.0	0.0	5.899694573110297	0.0
29	0.0	0.0	0.0	6.100447015514068	0.0
30	0.0	0.0	0.0	6.330528530107809	0.0
31	0.0	0.0	0.0	6.5398774247051925	0.0
32	0.0	0.0	0.0	6.757317097837739	0.0
33	0.0	0.0	0.0	6.961103582192196	0.0
34	0.0	0.0	0.0	7.171463824106475	0.0
35	0.0	0.0	0.0	7.377778676753171	0.0
36	0.0	0.0	0.0	7.616456643540524	0.0
37	5.056736584477841E-4	0.0	0.0	7.82985092740549	0.0
38	5.056736584477841E-4	0.0	0.0	8.044762232245798	0.0
39	5.056736584477841E-4	0.0	0.0	8.278889136107122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGAG	20	7.0191117E-4	45.0	2
GTTAACG	20	7.0191117E-4	45.0	1
GCGATGC	40	6.7666406E-9	45.0	9
TTAACGG	25	3.8790597E-5	45.0	2
TAGGCGA	25	3.8790597E-5	45.0	6
CGTTTGG	20	7.0191117E-4	45.0	2
ACACGAC	55	1.8189894E-12	44.999996	26
CACGACC	55	1.8189894E-12	44.999996	27
AGGCGAT	55	1.8189894E-12	44.999996	7
CGACCAA	60	3.6379788E-12	41.249996	29
GGCCGAT	40	3.4388904E-7	39.375	8
CCTGGGA	40	3.4388904E-7	39.375	4
ATACGAA	40	3.4388904E-7	39.375	19
GGCCTAT	40	3.4388904E-7	39.375	8
GCACGGG	70	0.0	38.571426	3
GCGATGA	70	0.0	38.571426	9
GCGAGAC	65	9.094947E-12	38.076927	21
CGAGACA	65	9.094947E-12	38.076927	22
CCGGGAT	30	1.1365073E-4	37.499996	5
CGAGATA	85	0.0	37.058823	19
>>END_MODULE