SRR2936512.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936512.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245646
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	2286	0.9306074595149116	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	1498	0.6098206362000602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	1197	0.4872865831318239	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.27559984693420614	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	658	0.267865139265447	RNA PCR Primer, Index 27 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	480	0.19540314110549328	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	376	0.15306579386596972	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	361	0.14695944570642308	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	357	0.14533108619721063	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	352	0.14329563681069507	RNA PCR Primer, Index 27 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTT	328	0.1335254797554204	RNA PCR Primer, Index 38 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	309	0.12579077208666128	RNA PCR Primer, Index 38 (95% over 21bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	273	0.1111355365037493	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCT	30	2.1585092E-6	45.000004	9
TTGTGCG	25	3.8818223E-5	45.0	1
TAAGTAG	20	7.022445E-4	45.0	1
AAACGAT	20	7.022445E-4	45.0	19
TGCGATG	20	7.022445E-4	45.0	1
CGAGGGT	25	3.8818223E-5	45.0	4
TTGACGG	20	7.022445E-4	45.0	2
GCGATTT	20	7.022445E-4	45.0	9
TGCTCGA	20	7.022445E-4	45.0	12
ACATCGC	20	7.022445E-4	45.0	10
CGTTTTA	25	3.8818223E-5	45.0	1
AGGATTG	90	0.0	42.5	7
TACGGCT	160	0.0	42.1875	7
AAACGGC	80	0.0	42.1875	6
GCACCGA	70	0.0	41.785717	9
ACGACCA	120	0.0	41.250004	28
CTACGGG	50	1.0750227E-9	40.5	3
GCGAGAC	125	0.0	39.600002	21
ACACGAC	125	0.0	39.600002	26
CACGACC	125	0.0	39.600002	27