##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936510.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50787
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910390454250102	31.0	31.0	34.0	30.0	34.0
2	32.02734951857759	33.0	31.0	34.0	30.0	34.0
3	31.920964026227185	33.0	31.0	34.0	30.0	34.0
4	35.67816567231772	37.0	35.0	37.0	33.0	37.0
5	35.75149152342135	37.0	35.0	37.0	35.0	37.0
6	35.72829661133755	37.0	35.0	37.0	35.0	37.0
7	26.92622127709847	35.0	15.0	37.0	0.0	37.0
8	31.175950538523637	35.0	19.0	37.0	17.0	37.0
9	35.91954634059897	39.0	32.0	39.0	32.0	39.0
10	36.671352905271036	38.0	35.0	39.0	33.0	39.0
11	36.991257605292695	39.0	37.0	39.0	34.0	39.0
12	36.823517829365784	39.0	35.0	39.0	33.0	39.0
13	36.51131194990844	39.0	35.0	39.0	33.0	39.0
14	37.58046350444011	39.0	36.0	41.0	33.0	41.0
15	37.73786598932798	40.0	36.0	41.0	33.0	41.0
16	37.78165672317719	40.0	36.0	41.0	33.0	41.0
17	37.70348711284384	39.0	35.0	41.0	33.0	41.0
18	37.588930238053045	39.0	36.0	41.0	33.0	41.0
19	37.48563608797527	39.0	36.0	41.0	33.0	41.0
20	37.59477819126942	39.0	35.0	41.0	33.0	41.0
21	37.50908697107528	39.0	35.0	41.0	33.0	41.0
22	37.52952527221533	39.0	35.0	41.0	33.0	41.0
23	37.500974658869396	39.0	35.0	41.0	33.0	41.0
24	37.46478429519365	39.0	35.0	41.0	33.0	41.0
25	37.3560950636974	39.0	35.0	41.0	33.0	41.0
26	37.187370783862015	39.0	35.0	41.0	33.0	41.0
27	37.03685982633351	39.0	35.0	40.0	32.0	41.0
28	37.03305964124677	39.0	35.0	41.0	32.0	41.0
29	36.94687616909839	39.0	35.0	41.0	32.0	41.0
30	36.90511351330065	39.0	35.0	41.0	32.0	41.0
31	36.771004390887434	39.0	35.0	40.0	31.0	41.0
32	36.49199598322405	38.0	35.0	40.0	31.0	41.0
33	36.49000728532892	38.0	35.0	40.0	31.0	41.0
34	36.32449248823518	38.0	35.0	40.0	30.0	41.0
35	36.286352806820645	38.0	35.0	40.0	30.0	41.0
36	36.119499084411366	38.0	35.0	40.0	30.0	41.0
37	35.983263433555834	38.0	35.0	40.0	30.0	41.0
38	35.90745663260283	38.0	35.0	40.0	30.0	41.0
39	35.77248114675015	38.0	35.0	40.0	29.0	41.0
40	35.68403331561226	38.0	35.0	40.0	29.0	41.0
41	35.67277059089925	38.0	35.0	40.0	29.0	41.0
42	35.66014925079253	38.0	35.0	40.0	29.0	41.0
43	35.64069545356095	38.0	35.0	40.0	29.0	41.0
44	35.364089235434264	38.0	34.0	40.0	28.0	41.0
45	35.192234233169906	37.0	34.0	40.0	27.0	41.0
46	35.11065823931321	37.0	34.0	40.0	27.0	41.0
47	34.95394490716128	37.0	34.0	40.0	26.0	41.0
48	34.90196310079351	37.0	34.0	40.0	26.0	41.0
49	34.71630535373225	37.0	34.0	40.0	25.0	41.0
50	34.42142674306417	37.0	34.0	40.0	24.0	41.0
51	32.01163683619824	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	3.0
12	3.0
13	1.0
14	1.0
15	0.0
16	3.0
17	10.0
18	15.0
19	31.0
20	56.0
21	104.0
22	117.0
23	229.0
24	336.0
25	407.0
26	534.0
27	585.0
28	715.0
29	874.0
30	1122.0
31	1467.0
32	1864.0
33	2800.0
34	4611.0
35	5884.0
36	4831.0
37	7155.0
38	8741.0
39	8280.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17982948392305	19.424262114320594	23.19097406816705	16.204934333589303
2	27.085671530115974	27.729537086262233	28.643156713332154	16.54163467028964
3	30.79528225727056	26.3315415362199	27.47159706224034	15.401579144269203
4	28.1607497981767	24.730738181030578	29.09799751905015	18.010514501742573
5	27.006911217437533	27.861460609998623	25.799909425640422	19.331718746923425
6	25.868824699234054	35.046370134089436	24.42947998503554	14.655325181640972
7	59.676688916455	27.316045444700414	8.758146769842675	4.24911886900191
8	80.5206056668045	6.084234154409593	8.622285230472365	4.772874948313545
9	75.17278043593834	6.259475850119125	10.108886132277945	8.4588575816646
10	46.1496052139327	22.69478409829287	17.234725421859924	13.920885265914507
11	37.17092956859039	21.720125228897157	23.813180538326737	17.295764664185718
12	33.049796207690946	21.05656959458129	27.432216905901118	18.46141729182665
13	23.803335499241932	29.9899580601335	29.253549136590074	16.953157304034498
14	17.118553960659224	34.248921968220216	28.14499773564101	20.487526335479554
15	15.181050268769566	24.815405517159903	42.59554610431803	17.407998109752494
16	17.726977376100184	23.443007068738062	36.74562388012681	22.08439167503495
17	18.67210112824148	21.64924094748656	30.03721424774056	29.6414436765314
18	21.28300549353181	23.431193021836297	32.82532931655739	22.460472168074507
19	23.320928584086477	24.785870399905487	29.310650363281944	22.58255065272609
20	25.99090318388564	24.703172071593123	29.38547266032646	19.92045208419477
21	22.553015535471676	27.56020241400358	29.47604701990667	20.41073503061807
22	20.973871266268926	25.201331049284263	27.583830507807118	26.24096717663969
23	19.18995018410223	28.31827042353358	27.755134187882724	24.736645204481462
24	21.95246815129856	22.84639770019887	31.12016854706913	24.080965601433437
25	18.40628507295174	27.678342883021244	30.289247248311575	23.626124795715437
26	18.84537381613405	28.942445901510226	28.223758048319453	23.988422234036268
27	21.180617087049836	29.56859038730384	28.01110520408766	21.239687321558666
28	18.15425207238073	27.349518577588753	33.707444818555935	20.78878453147459
29	19.51680548171776	24.303463484750036	32.95331482465985	23.22641620887235
30	23.143717880559986	25.904266839939353	30.20654891999921	20.745466359501446
31	26.607202630594447	27.178214897513143	24.992616220686394	21.221966251206016
32	25.2604012837931	27.262882233642465	27.426309882450234	20.0504066001142
33	25.84322759761356	24.687420009057437	26.04800441057751	23.42134798275149
34	22.1021127453876	27.73938212534704	27.87327465690039	22.285230472364976
35	21.00143737570638	24.606690688562033	29.885600645834565	24.506271289897022
36	27.028570303424104	24.813436509342942	26.729281115246028	21.428712071986926
37	22.854273731466716	25.45533305767224	31.655738673282535	20.034654537578515
38	22.31476558961939	26.581605528973952	27.987477110284125	23.11615177112253
39	22.210408175320456	25.522279323448917	29.830468426959655	22.436844074270976
40	24.069151554531672	24.6185047354638	28.83218146376041	22.480162246244117
41	19.28446255931636	25.091066611534448	27.568078445271425	28.056392383877764
42	22.363990785043416	24.224703172071592	29.15312973792506	24.25817630495993
43	25.349006635556343	23.472542185992477	25.794002402189538	25.384448776261642
44	22.340362691239886	25.91804989465808	28.04654734479296	23.695040069309076
45	20.245338373993345	26.494969185027667	27.01675625652234	26.24293618445665
46	25.24268021344045	27.369208655758364	26.034221355858783	21.353889774942406
47	20.664737039006045	24.669698938704787	31.95699686927757	22.708567153011597
48	22.779451434422196	22.480162246244117	29.127532636304565	25.612853683029122
49	21.789040502490796	20.42648709315376	32.75050701951287	25.033965384842578
50	20.934491109929706	23.53555043613523	30.75984011656526	24.770118337369798
51	19.83381574024849	24.716955126311852	26.56782247425522	28.88140665918444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	57.0
2	92.0
3	173.5
4	255.0
5	193.5
6	132.0
7	124.0
8	116.0
9	116.5
10	117.0
11	109.5
12	102.0
13	105.5
14	109.0
15	102.5
16	96.0
17	92.0
18	88.0
19	103.0
20	118.0
21	126.5
22	135.0
23	158.5
24	182.0
25	197.5
26	277.5
27	342.0
28	477.0
29	612.0
30	666.0
31	720.0
32	794.5
33	869.0
34	1003.5
35	1138.0
36	1256.0
37	1374.0
38	1611.0
39	1848.0
40	2167.0
41	2486.0
42	3009.0
43	3532.0
44	3989.5
45	4447.0
46	5396.5
47	6346.0
48	5829.5
49	5313.0
50	5114.0
51	4915.0
52	4265.5
53	3616.0
54	3162.5
55	2709.0
56	2374.0
57	2039.0
58	1796.5
59	1554.0
60	1480.0
61	1406.0
62	1333.0
63	1260.0
64	1039.0
65	818.0
66	711.0
67	604.0
68	502.0
69	400.0
70	303.0
71	206.0
72	201.5
73	197.0
74	159.5
75	94.0
76	66.0
77	50.5
78	35.0
79	27.0
80	19.0
81	15.0
82	11.0
83	6.0
84	1.0
85	1.5
86	2.0
87	2.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	50787.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.49768641581507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59395394193984	38.1101462972808
2	10.464331257970144	10.987063618642566
3	5.2771735053634385	8.311181995392522
4	3.2818243192558696	6.891527359363617
5	2.3029030080264046	6.044853998070372
6	1.8715775260670617	5.895209403981334
7	1.260220538594254	4.631106385492351
8	0.9301627784862351	3.90651150885069
9	0.6676168329457656	3.1543505227715753
>10	1.308979071337484	8.693169511882962
>50	0.018753281824319255	0.7974481658692184
>100	0.022503938189183108	2.5774312324019926
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	329	0.64780357178018	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	323	0.6359895248784138	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	288	0.5670742512847776	Illumina PCR Primer Index 5 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	143	0.2815681178254278	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	119	0.23431193021836294	Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.21068383641483054	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	96	0.1890247504282592	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	85	0.16736566444168782	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	81	0.1594896331738437	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	75	0.1476755862720775	Illumina PCR Primer Index 5 (96% over 26bp)
GAATCTATCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	68	0.13389253155335026	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18508673479433713	0.0
2	0.0	0.0	0.0	1.1361175103865162	0.0
3	0.0	0.0	0.0	1.4472207454663595	0.0
4	0.0	0.0	0.0	1.8725264339299428	0.0
5	0.0	0.0	0.0	3.5146789532754443	0.0
6	0.0	0.0	0.0	4.176265579774352	0.0
7	0.0	0.0	0.0	4.971744737826609	0.0
8	0.0	0.0	0.0	6.334298147163644	0.0
9	0.0	0.0	0.0	6.795045976332526	0.0
10	0.0	0.0	0.0	8.742394707306987	0.0
11	0.0	0.0	0.0	10.045877882135192	0.0
12	0.0	0.0	0.0	12.095615019591628	0.0
13	0.0	0.0	0.0	12.658751255242484	0.0
14	0.0	0.0	0.0	12.904877232362612	0.0
15	0.0	0.0	0.0	13.403036210053754	0.0
16	0.0	0.0	0.0	14.19457735247209	0.0
17	0.0	0.0	0.0	15.114104002992892	0.0
18	0.0	0.0	0.0	16.27384960718294	0.0
19	0.0	0.0	0.0	16.80745072557938	0.0
20	0.0	0.0	0.0	17.3016716876366	0.0
21	0.0	0.0	0.0	17.941599228148934	0.0
22	0.0	0.0	0.0	18.585464784295194	0.0
23	0.0	0.0	0.0	19.26674148896371	0.0
24	0.0	0.0	0.0	19.7826215370075	0.0
25	0.0	0.0	0.0	20.27093547561384	0.0
26	0.0	0.0	0.0	20.63323291393467	0.0
27	0.0	0.0	0.0	21.00340638352334	0.0
28	0.0	0.0	0.0	21.39917695473251	0.0
29	0.0	0.0	0.0	21.789040502490796	0.0
30	0.0	0.0	0.0	22.23600527694095	0.0
31	0.0	0.0	0.0	22.73416425463209	0.0
32	0.0	0.0	0.0	23.15356291964479	0.0
33	0.0	0.0	0.0	23.559178529938762	0.0
34	0.0	0.0	0.0	23.956918108964892	0.0
35	0.0	0.0	0.0	24.455077086656033	0.0
36	0.0	0.0	0.0	24.86659972040089	0.0
37	0.0	0.0	0.0	25.297812432315357	0.0
38	0.0	0.0	0.0	25.738870183314628	0.0
39	0.0	0.0	0.0	26.14251678579164	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGACC	20	6.969669E-4	45.0	8
ACACGCG	20	6.969669E-4	45.0	36
GTCACTT	20	6.969669E-4	45.0	4
GCAAGAC	20	6.969669E-4	45.0	45
GGCGATT	25	3.838173E-5	45.0	8
TTACACG	20	6.969669E-4	45.0	34
TACACGC	20	6.969669E-4	45.0	35
TAGTAGG	20	6.969669E-4	45.0	2
GAGTACC	20	6.969669E-4	45.0	30
CGGGAAT	20	6.969669E-4	45.0	6
GTCTTAC	20	6.969669E-4	45.0	31
ACAAGCG	25	3.838173E-5	45.0	14
CGAGATA	20	6.969669E-4	45.0	19
CTGTCAC	20	6.969669E-4	45.0	2
TGACGGG	25	3.838173E-5	45.0	3
CAAGCGA	20	6.969669E-4	45.0	15
GCGAGAT	20	6.969669E-4	45.0	18
GTCGGGA	25	3.838173E-5	45.0	4
GGGCGAT	65	0.0	41.53846	7
AGAGCTA	100	0.0	40.5	45
>>END_MODULE