##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936509.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224762 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.088991911444104 33.0 31.0 34.0 30.0 34.0 2 32.22452193876189 33.0 31.0 34.0 30.0 34.0 3 32.14035290663013 33.0 31.0 34.0 30.0 34.0 4 35.84991680088271 37.0 35.0 37.0 35.0 37.0 5 35.90726190370258 37.0 35.0 37.0 35.0 37.0 6 35.87583755261121 37.0 35.0 37.0 35.0 37.0 7 26.753045443624814 36.0 10.0 37.0 0.0 37.0 8 31.156725781048397 36.0 19.0 37.0 17.0 37.0 9 36.01983876278018 39.0 32.0 39.0 32.0 39.0 10 36.739177441026506 38.0 35.0 39.0 33.0 39.0 11 37.072178571110776 39.0 37.0 39.0 34.0 39.0 12 37.10710885291998 39.0 37.0 39.0 34.0 39.0 13 37.06848132691469 39.0 37.0 39.0 33.0 39.0 14 38.21504079871153 40.0 37.0 41.0 33.0 41.0 15 38.33170197809238 40.0 38.0 41.0 34.0 41.0 16 38.28204055845739 40.0 37.0 41.0 34.0 41.0 17 38.204803303049445 40.0 37.0 41.0 34.0 41.0 18 38.117995924578 40.0 37.0 41.0 33.0 41.0 19 38.010428809140336 40.0 37.0 41.0 33.0 41.0 20 38.05808811097961 40.0 37.0 41.0 34.0 41.0 21 37.9936466128616 40.0 37.0 41.0 33.0 41.0 22 37.99443411252792 40.0 37.0 41.0 34.0 41.0 23 37.95954832222529 40.0 36.0 41.0 34.0 41.0 24 37.9184604159066 40.0 36.0 41.0 33.0 41.0 25 37.80023758464509 40.0 36.0 41.0 33.0 41.0 26 37.68256644806507 40.0 36.0 41.0 33.0 41.0 27 37.54465612514571 40.0 36.0 41.0 33.0 41.0 28 37.51639512017156 40.0 36.0 41.0 33.0 41.0 29 37.494202756693745 40.0 36.0 41.0 33.0 41.0 30 37.38435767611963 40.0 36.0 41.0 33.0 41.0 31 37.279638017102535 40.0 35.0 41.0 32.0 41.0 32 37.16519696389959 39.0 35.0 41.0 32.0 41.0 33 37.060481754033155 39.0 35.0 41.0 31.0 41.0 34 36.94122227066853 39.0 35.0 41.0 31.0 41.0 35 36.843283117252916 39.0 35.0 41.0 31.0 41.0 36 36.69310203682117 39.0 35.0 41.0 31.0 41.0 37 36.63034231765156 39.0 35.0 41.0 30.0 41.0 38 36.52602753134427 39.0 35.0 41.0 30.0 41.0 39 36.484192167715186 39.0 35.0 41.0 30.0 41.0 40 36.32804477625221 39.0 35.0 41.0 30.0 41.0 41 36.25597298475721 39.0 35.0 40.0 30.0 41.0 42 36.157637856933114 39.0 35.0 40.0 30.0 41.0 43 36.094388731191216 39.0 35.0 40.0 29.0 41.0 44 35.92654897180128 38.0 35.0 40.0 29.0 41.0 45 35.82410282876999 38.0 35.0 40.0 29.0 41.0 46 35.72762299676992 38.0 35.0 40.0 28.0 41.0 47 35.59956309340547 38.0 34.0 40.0 28.0 41.0 48 35.53623388295174 38.0 34.0 40.0 28.0 41.0 49 35.394470595563305 38.0 34.0 40.0 27.0 41.0 50 35.20702342922736 38.0 34.0 40.0 26.0 41.0 51 33.117279611322196 36.0 31.0 39.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 7.0 9 30.0 10 20.0 11 14.0 12 9.0 13 6.0 14 8.0 15 12.0 16 32.0 17 62.0 18 100.0 19 172.0 20 261.0 21 390.0 22 633.0 23 891.0 24 1195.0 25 1607.0 26 1989.0 27 2320.0 28 2779.0 29 3255.0 30 4201.0 31 5295.0 32 6947.0 33 10022.0 34 15752.0 35 19523.0 36 20883.0 37 34072.0 38 43553.0 39 48711.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48165615184061 19.37382653651418 24.456981162296117 14.68753614934909 2 29.06719107322412 24.802680168355863 28.24454311671902 17.885585641701 3 32.55488027335582 24.244311760884845 27.090878351322733 16.109929614436606 4 28.80736067484717 25.740561126880877 26.599247203708813 18.85283099456314 5 25.665815395840934 29.970813571689163 25.192425765921282 19.170945266548618 6 26.52806079319458 35.471743444176504 24.04543472651071 13.954761036118205 7 62.8580453991333 28.251661757770442 5.267349462987516 3.6229433801087376 8 86.15246349471886 4.352604087879624 6.246162607558217 3.2487698098433007 9 79.80841957270357 6.866374209163471 8.42535659942517 4.899849618707789 10 43.4802146270277 27.167403742625535 15.31976045772862 14.032621172618148 11 31.67572810350504 26.06267963445778 24.599353983324583 17.662238278712593 12 28.362445609133218 24.551303156227476 27.744903497922245 19.34134773671706 13 24.69901495804451 26.045327946894936 29.632233206680848 19.62342388837971 14 20.869630987444495 27.80674669205649 30.348546462480314 20.975075858018705 15 20.274334629519224 26.334077824543296 33.89362970608911 19.49795783984837 16 24.69812512791308 25.138146127904182 30.179923652574725 19.983805091608012 17 25.272510477749798 24.557976882213186 27.512657833619564 22.656854806417453 18 25.588845089472418 24.78043441507016 28.72193698223009 20.908783513227323 19 25.96168391454071 25.813082282592255 26.658420907448765 21.566812895418263 20 27.01613262028279 26.707806479743017 26.718929356385868 19.55713154358833 21 25.811302622329396 26.18280670220055 28.46522098931314 19.540669686156914 22 24.895667417090078 24.22562532812486 28.33352612986181 22.545181124923253 23 23.554693409028214 25.78327297318942 28.434521849778875 22.227511768003488 24 23.358040949982648 24.66208700759025 30.03221185075769 21.94766019166941 25 22.876197933814435 27.04149277902848 27.869034801256444 22.21327448590064 26 22.37611339995195 27.210560503999787 27.90952207223641 22.503804023811856 27 22.06378302382075 26.63973447468878 28.953292816401348 22.343189685089115 28 21.402639236169815 26.73005223302872 29.811533978163567 22.055774552637903 29 22.9197996102544 26.003950845783542 29.23358930780114 21.842660236160917 30 23.39185449497691 25.65958658492094 29.060962262304123 21.887596657798024 31 24.48323115117324 25.9834847527607 27.22123846557692 22.312045630489138 32 26.235306679954796 26.547192141020282 26.518717576814588 20.698783602210337 33 24.160667728530623 26.121408423132024 27.31333588417971 22.404587964157642 34 23.350922308931224 26.272234630409052 28.300157499933263 22.076685560726457 35 22.257321077406324 27.233696087416913 29.279415559569678 21.229567275607085 36 24.693675977255943 27.25104777497976 27.493971400859575 20.561304846904726 37 23.48751123410541 27.80630177699077 27.536683247168114 21.169503741735703 38 22.486897251314726 28.01852626333633 27.24170455859976 22.252871926749187 39 23.128019861008532 27.170073233019814 27.267954547476887 22.433952358494764 40 23.929756809425083 25.89450173961791 27.68528487911658 22.490456571840436 41 22.318274441409137 25.77081535134943 27.780941618245077 24.129968588996363 42 23.501748516208256 25.609311182495258 28.360665948870363 22.528274352426124 43 23.3438036678798 25.09632411172707 28.18848381843906 23.371388401954064 44 22.5211557113747 25.952340698160718 28.823822532278587 22.70268105818599 45 22.13674909459784 26.556090442334558 27.500200211779568 23.80696025128803 46 23.25259607940844 26.638399729491642 27.504204447370995 22.604799743728922 47 22.024185582972212 26.35142951210614 29.423123125795286 22.201261779126366 48 22.50647351420614 25.951895783095008 28.768653064130056 22.772977638568797 49 22.90289283775727 25.17507407835844 29.336364687981064 22.585668395903223 50 21.714524697235298 26.40882355558324 28.75174629163293 23.124905455548536 51 21.72386791361529 26.0186330429521 28.152445698116228 24.105053345316378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 220.0 2 384.0 3 615.0 4 846.0 5 613.0 6 380.0 7 360.5 8 341.0 9 343.5 10 346.0 11 345.0 12 344.0 13 348.5 14 353.0 15 375.5 16 398.0 17 446.0 18 494.0 19 513.0 20 532.0 21 595.0 22 658.0 23 782.5 24 907.0 25 1138.5 26 1921.5 27 2473.0 28 2935.5 29 3398.0 30 3899.5 31 4401.0 32 5070.0 33 5739.0 34 6451.5 35 7164.0 36 7992.0 37 8820.0 38 9403.5 39 9987.0 40 10780.5 41 11574.0 42 12661.5 43 13749.0 44 14929.0 45 16109.0 46 16829.0 47 17549.0 48 17470.5 49 17392.0 50 17168.5 51 16945.0 52 15869.0 53 14793.0 54 13851.0 55 12909.0 56 12267.5 57 11626.0 58 10898.0 59 10170.0 60 9466.5 61 8763.0 62 8086.0 63 7409.0 64 6517.5 65 5626.0 66 4693.0 67 3760.0 68 3166.5 69 2573.0 70 2150.5 71 1728.0 72 1371.0 73 1014.0 74 877.0 75 567.0 76 394.0 77 322.0 78 250.0 79 184.0 80 118.0 81 98.0 82 78.0 83 65.0 84 52.0 85 35.0 86 18.0 87 15.5 88 13.0 89 12.0 90 11.0 91 7.5 92 4.0 93 3.0 94 2.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 224762.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.11299063008872 #Duplication Level Percentage of deduplicated Percentage of total 1 66.02045344095336 27.803187371530775 2 10.043949542544425 8.459615059485143 3 6.0208760327086015 7.606712878511492 4 4.1572463921228895 7.002963134337654 5 3.00885329727217 6.3355905357667215 6 2.360174952986667 5.9636415408298555 7 1.8340482177192725 5.406607878555984 8 1.4220212563652883 4.79084542760787 9 1.152619012403068 4.368621030245326 >10 3.967080102267205 21.641113711392496 >50 0.007395355716609969 0.2304660040398288 >100 0.005282396940435692 0.3906354276968527 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.16239399898559367 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0338135449942606 0.0 2 0.0 0.0 0.0 0.23002108897411483 0.0 3 0.0 0.0 0.0 0.3096608857369128 0.0 4 0.0 0.0 0.0 0.41688541657397604 0.0 5 0.0 0.0 0.0 0.8012920333508333 0.0 6 0.0 0.0 0.0 1.0873724206049065 0.0 7 0.0 0.0 0.0 1.3436434984561447 0.0 8 0.0 0.0 0.0 1.8824356430357445 0.0 9 4.449150657139552E-4 0.0 0.0 2.12268977852128 0.0 10 4.449150657139552E-4 0.0 0.0 2.6276683781066192 0.0 11 4.449150657139552E-4 0.0 0.0 3.1326469776919588 0.0 12 4.449150657139552E-4 0.0 0.0 3.606036607611607 0.0 13 4.449150657139552E-4 0.0 0.0 3.7991297461314635 0.0 14 4.449150657139552E-4 0.0 0.0 3.894786485259964 0.0 15 4.449150657139552E-4 0.0 0.0 4.0416084569455695 0.0 16 4.449150657139552E-4 0.0 0.0 4.300549025191091 0.0 17 4.449150657139552E-4 0.0 0.0 4.633790409410843 0.0 18 4.449150657139552E-4 0.0 0.0 4.997286018099145 0.0 19 4.449150657139552E-4 0.0 0.0 5.233091002927541 0.0 20 4.449150657139552E-4 0.0 0.0 5.46578158229594 0.0 21 4.449150657139552E-4 0.0 0.0 5.745188243564304 0.0 22 4.449150657139552E-4 0.0 0.0 6.058853364892642 0.0 23 4.449150657139552E-4 0.0 0.0 6.354721883592422 0.0 24 4.449150657139552E-4 0.0 0.0 6.618556517560798 0.0 25 4.449150657139552E-4 0.0 0.0 6.846353031206343 0.0 26 8.898301314279104E-4 0.0 0.0 7.054128366894759 0.0 27 8.898301314279104E-4 0.0 0.0 7.293492672248868 0.0 28 8.898301314279104E-4 0.0 0.0 7.523513761222983 0.0 29 8.898301314279104E-4 0.0 0.0 7.782899244534218 0.0 30 8.898301314279104E-4 0.0 0.0 8.083216913891139 0.0 31 8.898301314279104E-4 0.0 0.0 8.357284594370935 0.0 32 8.898301314279104E-4 0.0 0.0 8.604657370907894 0.0 33 8.898301314279104E-4 0.0 0.0 8.846246251590571 0.0 34 8.898301314279104E-4 0.0 0.0 9.113640206084659 0.0 35 8.898301314279104E-4 0.0 0.0 9.405949404258727 0.0 36 8.898301314279104E-4 0.0 0.0 9.661775567044252 0.0 37 8.898301314279104E-4 0.0 0.0 9.942516973509758 0.0 38 8.898301314279104E-4 0.0 0.0 10.257516840035237 0.0 39 8.898301314279104E-4 0.0 0.0 10.683300557923493 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCGT 20 7.021165E-4 45.000004 28 TAGACGG 20 7.021165E-4 45.000004 2 ACCGATA 30 2.1576852E-6 45.000004 10 CACGACC 20 7.021165E-4 45.000004 27 ACCTCGA 20 7.021165E-4 45.000004 28 ATCGTGA 20 7.021165E-4 45.000004 30 GTTGTAG 30 2.1576852E-6 45.000004 1 GTATACG 20 7.021165E-4 45.000004 1 TCAACGG 20 7.021165E-4 45.000004 2 ACGGGTA 25 3.880763E-5 45.0 5 ATGGGAC 130 0.0 39.80769 5 GACCGAT 130 0.0 39.80769 9 TATACGG 40 3.4416917E-7 39.375004 2 TCGCGGG 40 3.4416917E-7 39.375004 3 GGACCGA 150 0.0 39.0 8 CATGCGG 35 6.225886E-6 38.571426 2 GCGATAA 35 6.225886E-6 38.571426 9 TCGTACG 35 6.225886E-6 38.571426 30 ACCGGCG 30 1.1370021E-4 37.500004 20 CACGCCA 30 1.1370021E-4 37.500004 27 >>END_MODULE