##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936507.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244677
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03338687330644	33.0	31.0	34.0	30.0	34.0
2	32.160485047634225	33.0	31.0	34.0	30.0	34.0
3	31.91745035291425	33.0	31.0	34.0	30.0	34.0
4	35.72455931697708	37.0	35.0	37.0	35.0	37.0
5	35.84784838787463	37.0	35.0	37.0	35.0	37.0
6	35.82885600199447	37.0	35.0	37.0	35.0	37.0
7	26.721919101509336	35.0	10.0	37.0	0.0	37.0
8	31.10726794917381	35.0	19.0	37.0	17.0	37.0
9	35.96669895413137	39.0	32.0	39.0	32.0	39.0
10	36.719438279854664	38.0	35.0	39.0	33.0	39.0
11	37.06278072724449	39.0	37.0	39.0	34.0	39.0
12	37.125471540030325	39.0	37.0	39.0	34.0	39.0
13	37.1026250934906	39.0	37.0	39.0	33.0	39.0
14	38.25476444455343	40.0	38.0	41.0	34.0	41.0
15	38.326847231247726	40.0	38.0	41.0	33.0	41.0
16	38.28535579559991	40.0	37.0	41.0	33.0	41.0
17	38.20551175631547	40.0	37.0	41.0	33.0	41.0
18	38.06156688205267	40.0	37.0	41.0	33.0	41.0
19	37.914303346861374	40.0	37.0	41.0	33.0	41.0
20	37.92639275452944	40.0	36.0	41.0	33.0	41.0
21	37.86531631497852	40.0	36.0	41.0	33.0	41.0
22	37.87876261356809	40.0	36.0	41.0	33.0	41.0
23	37.83519497132955	40.0	36.0	41.0	33.0	41.0
24	37.762531827674856	40.0	36.0	41.0	33.0	41.0
25	37.66291069450745	40.0	36.0	41.0	33.0	41.0
26	37.52762213040049	40.0	35.0	41.0	33.0	41.0
27	37.4037240933966	39.0	35.0	41.0	33.0	41.0
28	37.33751435566073	39.0	35.0	41.0	33.0	41.0
29	37.32123575162357	39.0	35.0	41.0	33.0	41.0
30	37.21594592054014	39.0	35.0	41.0	32.0	41.0
31	37.066050343922804	39.0	35.0	41.0	32.0	41.0
32	36.87555838922334	39.0	35.0	41.0	31.0	41.0
33	36.750054153026234	39.0	35.0	41.0	31.0	41.0
34	36.542122880368815	39.0	35.0	41.0	30.0	41.0
35	36.39127094087307	39.0	35.0	41.0	30.0	41.0
36	36.17700069888056	39.0	35.0	41.0	29.0	41.0
37	36.056413148763475	39.0	35.0	41.0	29.0	41.0
38	35.91041250301418	39.0	35.0	40.0	28.0	41.0
39	35.81480482431941	39.0	35.0	40.0	28.0	41.0
40	35.62748848481876	38.0	35.0	40.0	27.0	41.0
41	35.51718387915497	38.0	35.0	40.0	26.0	41.0
42	35.419025899451114	38.0	35.0	40.0	26.0	41.0
43	35.41052080906665	38.0	35.0	40.0	26.0	41.0
44	35.19132979397328	38.0	34.0	40.0	25.0	41.0
45	35.08456454836376	38.0	34.0	40.0	25.0	41.0
46	34.96987865635103	38.0	34.0	40.0	24.0	41.0
47	34.81969290125349	38.0	34.0	40.0	24.0	41.0
48	34.70622085443258	38.0	34.0	40.0	23.0	41.0
49	34.55481716712237	37.0	34.0	40.0	23.0	41.0
50	34.37148158592757	37.0	33.0	40.0	23.0	41.0
51	32.221038348516615	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	19.0
10	17.0
11	8.0
12	7.0
13	12.0
14	7.0
15	20.0
16	31.0
17	80.0
18	118.0
19	204.0
20	325.0
21	569.0
22	803.0
23	1146.0
24	1730.0
25	2452.0
26	3070.0
27	3673.0
28	3895.0
29	4279.0
30	4967.0
31	6332.0
32	7927.0
33	11032.0
34	17032.0
35	20920.0
36	23523.0
37	36554.0
38	45304.0
39	48599.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.339619171397395	18.066675658112533	22.957204804701707	19.636500365788365
2	30.12297845731311	25.167056977157642	27.076921819378203	17.633042746151048
3	31.03397540430854	23.960568422859524	28.80614034012188	16.199315832710063
4	26.948180662669564	25.005619653665857	29.216477233250366	18.82972245041422
5	25.397973655063616	28.68598192719381	26.66576752208013	19.250276895662445
6	25.728204939573395	33.77105326614271	26.269326499834477	14.231415294449418
7	59.34027309473306	28.16979119410488	8.474029026022063	4.015906685140001
8	81.80008746224614	4.904425017472014	9.460227156618727	3.835260363663115
9	75.99856136866154	6.286655468229544	11.405649080215959	6.309134082892958
10	42.797238808715164	22.45817955917393	19.085569955492343	15.659011676618562
11	30.80428483265693	24.261781859349266	25.825884737838045	19.108048570155756
12	27.121470346620235	22.194158012400024	30.561515794292067	20.122855846687674
13	23.65649407177626	23.235530924443246	32.925039950628786	20.182935053151706
14	20.934129484994504	24.73138055477221	32.44971942601879	21.884770534214496
15	20.193561307356227	23.85961900791656	35.31472104039203	20.632098644335183
16	24.00143863133846	23.9192895122958	30.825128638981187	21.254143217384552
17	24.821295013425864	24.089718281652956	28.979430024072556	22.10955668084863
18	24.649640137814345	23.949533466570212	30.310572714231416	21.090253681384027
19	24.576073762552262	25.843458927483987	27.636434973454797	21.944032336508947
20	25.42045226972703	25.636655672580588	29.04727457014758	19.895617487544804
21	25.034228799601106	25.93419079030722	29.185415874806374	19.846164535285293
22	24.512724939409917	23.15256440123101	29.44943742158029	22.885273237778787
23	22.860342410606638	24.81148616339092	29.7249026267283	22.603268799274147
24	22.99439669441754	23.75989569922796	30.83575489318571	22.409952713168792
25	22.909795362866145	25.946043150766112	28.287088692439422	22.85707279392832
26	22.68705272665596	25.08899487896288	28.850280165279123	23.37367222910204
27	22.836637689688853	24.78737274038835	29.36728830253763	23.008701267385163
28	21.02853966658084	25.203840164788684	30.59543806732958	23.172182101300898
29	22.749992847713514	24.081135537872377	30.049003379966244	23.119868234447864
30	23.722703809512133	24.467359007998297	29.680354099486262	22.129583083003308
31	23.817522693183257	24.0611091357177	29.48172488627864	22.639643284820394
32	24.552777743719272	24.6819276025127	28.522092391193286	22.24320226257474
33	24.268321092705893	24.373357528496754	27.803594126133635	23.554727252663714
34	21.929319061456532	24.74486772357026	30.73766639283627	22.588146822136938
35	22.85053356057169	25.211605504399675	29.010082680431754	22.927778254596877
36	23.47135202736669	25.538158470146357	27.97974472467784	23.010744777809112
37	22.443874986206307	26.429946419156686	28.90586364881047	22.220314945826537
38	22.221132349996118	25.56799372233598	28.247035888130068	23.96383803953784
39	22.613486351393878	25.289258900509655	28.234366123501598	23.862888624594873
40	23.811800863996208	24.139579935997254	28.49143973483409	23.55717946517245
41	21.468711811898952	24.37989676185338	28.824531933937397	25.326859492310273
42	21.7695165463039	24.336165638780923	29.67054524945132	24.223772565463854
43	22.676017770366645	24.107292471298898	29.111440797459508	24.10524896087495
44	22.307368489886667	24.542560191599538	28.942238134356725	24.207833184157074
45	22.222767158335273	25.075916412249622	28.295262734135207	24.4060536952799
46	22.379708758894377	25.507914515871942	28.412968934554534	23.69940779067914
47	21.207959881803358	24.776746486183825	30.458114166840367	23.55717946517245
48	22.193340608230443	24.42934971411289	29.724493924643507	23.652815753013158
49	22.0915737891179	24.19679822786776	29.82339982916253	23.888228153851813
50	21.37716254490614	24.75140695692689	30.17284011165741	23.69859038650956
51	21.534512847550037	24.09830102543353	28.737478389877268	25.629707737139167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	217.0
2	371.0
3	1884.5
4	3398.0
5	2268.0
6	1138.0
7	1016.5
8	895.0
9	831.5
10	768.0
11	790.0
12	812.0
13	781.0
14	750.0
15	740.5
16	731.0
17	731.5
18	732.0
19	732.0
20	732.0
21	798.0
22	864.0
23	984.0
24	1104.0
25	1347.5
26	1793.0
27	1995.0
28	2507.0
29	3019.0
30	3642.0
31	4265.0
32	4760.0
33	5255.0
34	5970.0
35	6685.0
36	7167.0
37	7649.0
38	8340.0
39	9031.0
40	9868.5
41	10706.0
42	11791.5
43	12877.0
44	14149.5
45	15422.0
46	17693.5
47	19965.0
48	19576.5
49	19188.0
50	18818.0
51	18448.0
52	16935.0
53	15422.0
54	14708.5
55	13995.0
56	13343.5
57	12692.0
58	12289.0
59	11886.0
60	11326.5
61	10767.0
62	9843.0
63	8919.0
64	8022.5
65	7126.0
66	6108.5
67	5091.0
68	4382.5
69	3674.0
70	3077.0
71	2480.0
72	2075.5
73	1671.0
74	1374.0
75	857.5
76	638.0
77	497.5
78	357.0
79	289.0
80	221.0
81	159.5
82	98.0
83	71.0
84	44.0
85	32.5
86	21.0
87	22.5
88	24.0
89	16.0
90	8.0
91	5.5
92	3.0
93	4.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	244677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.10187999695376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.77782093859675	37.05333473930292
2	12.16581712972531	12.920555225751157
3	6.458452894969362	10.28867971783925
4	3.8334723056647273	8.142583453882159
5	2.5335151754039624	6.726720940738123
6	1.7591765356130913	5.604934877254994
7	1.174320423532423	4.3651035545873444
8	0.7860433143004038	3.3392302198710277
9	0.4984345389834794	2.3821029967893925
>10	0.9930099453158927	6.531679391366491
>50	0.00613439935218925	0.24064643713465816
>100	0.011501998785354843	1.0953281148768705
>500	0.0015335998380473124	0.5729095381530694
>1k	7.667999190236562E-4	0.7361907924525584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1808	0.7389333692991168	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	776	0.31715281779652355	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	631	0.2578910155020701	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	399	0.16307213183094446	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.13160207130216572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07765339610997356	0.0
2	0.0	0.0	0.0	0.3600665366994037	0.0
3	0.0	0.0	0.0	0.5055644788844068	0.0
4	0.0	0.0	0.0	0.7193156692292287	0.0
5	0.0	0.0	0.0	1.4897190990571243	0.0
6	4.087020847893345E-4	0.0	0.0	2.072119569881926	0.0
7	4.087020847893345E-4	0.0	0.0	2.570327411240125	0.0
8	4.087020847893345E-4	0.0	0.0	3.429419193467306	0.0
9	4.087020847893345E-4	0.0	0.0	3.8589650845808965	0.0
10	4.087020847893345E-4	0.0	0.0	4.856198171466873	0.0
11	4.087020847893345E-4	0.0	0.0	5.506851890451493	0.0
12	4.087020847893345E-4	0.0	0.0	6.216358709645778	0.0
13	4.087020847893345E-4	0.0	0.0	6.454223322993171	0.0
14	4.087020847893345E-4	0.0	0.0	6.594816840160702	0.0
15	4.087020847893345E-4	0.0	0.0	6.774645757468009	0.0
16	4.087020847893345E-4	0.0	0.0	7.036623793817972	0.0
17	4.087020847893345E-4	0.0	0.0	7.362359355395072	0.0
18	4.087020847893345E-4	0.0	0.0	7.708938723296428	0.0
19	4.087020847893345E-4	0.0	0.0	7.932907465760983	0.0
20	4.087020847893345E-4	0.0	0.0	8.161371931158222	0.0
21	4.087020847893345E-4	0.0	0.0	8.451141709273859	0.0
22	4.087020847893345E-4	0.0	0.0	8.767068420816015	0.0
23	4.087020847893345E-4	0.0	0.0	9.08340383444296	0.0
24	4.087020847893345E-4	0.0	0.0	9.348242785386448	0.0
25	4.087020847893345E-4	0.0	0.0	9.587333504988209	0.0
26	4.087020847893345E-4	0.0	0.0	9.817841480809394	0.0
27	4.087020847893345E-4	0.0	0.0	10.048758158715367	0.0
28	4.087020847893345E-4	0.0	0.0	10.269048582416819	0.0
29	4.087020847893345E-4	0.0	0.0	10.535931043784254	0.0
30	4.087020847893345E-4	0.0	0.0	10.837553182358784	0.0
31	4.087020847893345E-4	0.0	0.0	11.083183135317173	0.0
32	4.087020847893345E-4	0.0	0.0	11.357422234210818	0.0
33	4.087020847893345E-4	0.0	0.0	11.603052187169206	0.0
34	4.087020847893345E-4	0.0	0.0	11.874839073554114	0.0
35	4.087020847893345E-4	0.0	0.0	12.163791447500174	0.0
36	4.087020847893345E-4	0.0	0.0	12.404925677525881	0.0
37	4.087020847893345E-4	0.0	0.0	12.667721118045423	0.0
38	4.087020847893345E-4	0.0	0.0	12.98896095668984	0.0
39	4.087020847893345E-4	0.0	0.0	13.409515401938066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCC	55	6.002665E-11	40.909092	37
TACTGAC	55	6.002665E-11	40.909092	20
CGTTTTA	150	0.0	40.500004	1
CGTTTTT	590	0.0	40.042374	1
CGGGTAT	40	3.443365E-7	39.375	6
GTTTGCG	35	6.228149E-6	38.571426	1
CACAACG	35	6.228149E-6	38.571426	12
ATTGCGG	35	6.228149E-6	38.571426	2
CGCGGGT	30	1.1372972E-4	37.499996	4
ATTCGGG	30	1.1372972E-4	37.499996	3
TACGAAT	60	1.546141E-10	37.499996	12
TCGTCCC	60	1.546141E-10	37.499996	38
GTCGTAG	30	1.1372972E-4	37.499996	1
CGAATAT	60	1.546141E-10	37.499996	14
GCGATCT	30	1.1372972E-4	37.499996	9
ACGTAAG	30	1.1372972E-4	37.499996	2
GCTACGA	60	1.546141E-10	37.499996	10
TAAGCGG	30	1.1372972E-4	37.499996	2
TTACACG	105	0.0	36.42857	34
CACGGGT	25	0.0021038747	36.000004	4
>>END_MODULE