##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	154597
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95563950141335	33.0	31.0	34.0	30.0	34.0
2	32.07479446561059	33.0	31.0	34.0	30.0	34.0
3	31.995148676882476	33.0	31.0	34.0	30.0	34.0
4	35.75320349036527	37.0	35.0	37.0	35.0	37.0
5	35.83296571084821	37.0	35.0	37.0	35.0	37.0
6	35.803081560444255	37.0	35.0	37.0	35.0	37.0
7	26.885224163470184	35.0	10.0	37.0	0.0	37.0
8	31.224752097388695	36.0	19.0	37.0	17.0	37.0
9	35.99262598886136	39.0	32.0	39.0	32.0	39.0
10	36.677529318162705	38.0	35.0	39.0	33.0	39.0
11	37.00679832079536	39.0	37.0	39.0	34.0	39.0
12	37.052129084005514	39.0	37.0	39.0	34.0	39.0
13	36.98873846193652	39.0	37.0	39.0	33.0	39.0
14	38.1104807984631	40.0	37.0	41.0	33.0	41.0
15	38.182442091373055	40.0	37.0	41.0	33.0	41.0
16	38.14470526594953	40.0	37.0	41.0	33.0	41.0
17	38.072375272482645	40.0	37.0	41.0	33.0	41.0
18	38.005103591919635	40.0	37.0	41.0	33.0	41.0
19	37.88087737795688	40.0	36.0	41.0	33.0	41.0
20	37.941700032989	40.0	36.0	41.0	33.0	41.0
21	37.8431793631183	40.0	36.0	41.0	33.0	41.0
22	37.83371604882372	40.0	36.0	41.0	33.0	41.0
23	37.770868774943885	39.0	36.0	41.0	33.0	41.0
24	37.74183198897779	40.0	36.0	41.0	33.0	41.0
25	37.57859466871932	39.0	36.0	41.0	33.0	41.0
26	37.39298951467364	39.0	35.0	41.0	32.0	41.0
27	37.24040569998124	39.0	35.0	41.0	32.0	41.0
28	37.18094141542203	39.0	35.0	41.0	32.0	41.0
29	37.11795830449491	39.0	35.0	41.0	32.0	41.0
30	37.02584138113935	39.0	35.0	41.0	31.0	41.0
31	36.82257094251506	39.0	35.0	41.0	31.0	41.0
32	36.68621642075849	39.0	35.0	41.0	30.0	41.0
33	36.56847804291157	39.0	35.0	41.0	30.0	41.0
34	36.46909060331054	39.0	35.0	41.0	30.0	41.0
35	36.43870191530237	39.0	35.0	41.0	30.0	41.0
36	36.317794006352	39.0	35.0	40.0	30.0	41.0
37	36.280425881485414	39.0	35.0	40.0	30.0	41.0
38	36.105377206543466	38.0	35.0	40.0	30.0	41.0
39	36.09698765176556	38.0	35.0	40.0	30.0	41.0
40	35.885049515837956	38.0	35.0	40.0	29.0	41.0
41	35.80050065654573	38.0	35.0	40.0	28.0	41.0
42	35.70481315937567	38.0	35.0	40.0	28.0	41.0
43	35.66742562921661	38.0	35.0	40.0	28.0	41.0
44	35.405551207332614	38.0	34.0	40.0	27.0	41.0
45	35.33065324682885	38.0	34.0	40.0	27.0	41.0
46	35.189512086262994	38.0	34.0	40.0	26.0	41.0
47	35.06969734212178	38.0	34.0	40.0	26.0	41.0
48	35.03315070796975	38.0	34.0	40.0	26.0	41.0
49	34.85521064444976	37.0	34.0	40.0	24.0	41.0
50	34.560204919888484	37.0	33.0	40.0	24.0	41.0
51	32.135714147105055	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	8.0
11	11.0
12	3.0
13	7.0
14	7.0
15	9.0
16	14.0
17	30.0
18	59.0
19	115.0
20	163.0
21	265.0
22	404.0
23	630.0
24	920.0
25	1211.0
26	1570.0
27	1953.0
28	2310.0
29	2754.0
30	3281.0
31	4271.0
32	5325.0
33	7558.0
34	11755.0
35	14806.0
36	15151.0
37	23149.0
38	28286.0
39	28556.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16444691682245	19.690550269410142	23.453883322444806	15.691119491322599
2	28.147376727879585	25.697781975070665	27.87570263329819	18.27913866375156
3	31.195301331849905	24.581977658039936	28.029004443811978	16.193716566298182
4	28.761877656099404	26.208788010116624	26.306461315549463	18.722873018234505
5	24.783792699728973	29.671339029864747	25.799336338997524	19.74553193140876
6	26.2100816962813	34.688900819550184	25.259222365246416	13.841795118922102
7	61.58981092776703	27.833657832946308	6.44126341390842	4.135267825378241
8	83.20148515171705	4.611991177060356	7.619811509925807	4.566712161296791
9	77.32232837635918	6.916046236343526	9.995019308266007	5.766606079031288
10	42.44066831827267	26.959125985627146	16.57276661254746	14.02743908355272
11	32.22766289125921	25.276687128469504	23.992056766948906	18.503593213322382
12	29.364735408837173	23.03990374974935	28.071696087246195	19.523664754167285
13	24.43967217992587	26.26441651519758	30.0199874512442	19.27592385363235
14	20.592896369269777	29.223076773805438	28.768346086922776	21.415680770002005
15	18.759743073927694	26.26765073060926	36.306008525391825	18.666597670071216
16	22.118152357419614	24.362051010045473	33.254202862927485	20.26559376960743
17	21.636254261078804	24.525702309876646	28.616984805656	25.221058623388554
18	23.330336293718506	24.746275800953445	30.283899428837557	21.639488476490488
19	24.630490889215185	27.01863554920212	26.832344741489162	21.518528820093533
20	27.22756586479686	26.075538335155272	26.908025382122553	19.788870417925317
21	24.49853490041851	26.96688810261519	27.97208225256635	20.562494744399956
22	22.779872830650014	25.336196692044478	26.79029994113728	25.09363053616823
23	21.578038383668506	27.63507700666895	27.598853794058098	23.188030815604442
24	22.510139265315626	24.666714101826038	29.505100357704222	23.31804627515411
25	21.62331739943207	26.804530488948686	27.99342807428346	23.578724037335782
26	20.07994980497681	28.508962010905776	28.578174220715795	22.83291396340162
27	21.912456257236556	28.021242326823938	27.97725699722504	22.089044418714465
28	19.7532940483968	27.216569532397134	30.319475798366074	22.710660620839988
29	21.005582255800565	24.84847700796264	30.41262120222255	23.733319534014242
30	21.737808625005663	24.950031371889494	29.195909364347305	24.116250638757545
31	22.62333680472454	25.970749755816737	26.832344741489162	24.57356869796956
32	23.181562384781078	25.664146134789164	28.380887080603117	22.773404399826646
33	22.521782440797686	26.048370925697135	27.389276635381023	24.040569998124155
34	20.221608440008538	24.838774361727587	31.148728629921667	23.790888568342204
35	19.475151522992036	25.098805280826923	29.71726488871065	25.708778307470393
36	21.451257139530522	25.673201937941876	28.812978259604005	24.0625626629236
37	20.87491995316856	26.171917954423435	28.742472363629307	24.210689728778696
38	19.984863871873323	26.877623757252728	28.113740887598077	25.02377148327587
39	21.41826814233135	24.49465384192449	28.460448779730523	25.62662923601364
40	22.70483903309896	23.019851614196913	30.543283504854557	23.73202584784957
41	18.75327464310433	24.570334482557875	29.456587126528976	27.219803747808818
42	21.568335737433454	24.327768326681632	29.588543115325656	24.51535282055926
43	22.41893439070616	24.111075894098853	28.032885502305994	25.437104212888993
44	20.87103889467454	25.86725486264287	28.554887869751678	24.70681837293091
45	20.526271531789103	25.23140811270594	27.619552772692874	26.622767582812084
46	21.922805746553944	25.49596693338163	27.90675110125035	24.67447621881408
47	19.70866187571557	25.094924222332903	30.175229791005	25.021184110946525
48	21.131069813773877	23.54444135397194	29.237954164699186	26.086534667555
49	21.131716656856213	23.055427983725426	30.162939772440605	25.649915586977755
50	19.22288272088074	24.485598038771776	29.849867720589664	26.44165151975782
51	19.12068151387155	24.897637082220225	27.487596783896194	28.494084620012032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	195.0
2	292.0
3	473.0
4	654.0
5	467.0
6	280.0
7	263.5
8	247.0
9	240.0
10	233.0
11	244.0
12	255.0
13	239.0
14	223.0
15	251.0
16	279.0
17	265.0
18	251.0
19	295.0
20	339.0
21	424.5
22	510.0
23	538.0
24	566.0
25	714.5
26	1082.0
27	1301.0
28	1622.0
29	1943.0
30	2309.5
31	2676.0
32	3045.0
33	3414.0
34	3755.5
35	4097.0
36	4585.0
37	5073.0
38	5385.5
39	5698.0
40	6851.5
41	8005.0
42	8860.5
43	9716.0
44	10700.5
45	11685.0
46	13099.0
47	14513.0
48	15011.0
49	15509.0
50	14804.0
51	14099.0
52	12416.5
53	10734.0
54	9572.5
55	8411.0
56	7629.0
57	6847.0
58	6699.5
59	6552.0
60	6305.0
61	6058.0
62	5301.0
63	4544.0
64	3774.5
65	3005.0
66	2469.5
67	1934.0
68	1601.0
69	1268.0
70	1143.0
71	1018.0
72	816.0
73	614.0
74	486.5
75	266.5
76	174.0
77	144.5
78	115.0
79	92.5
80	70.0
81	50.5
82	31.0
83	25.5
84	20.0
85	18.0
86	16.0
87	10.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	154597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.06567397814964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06318242849544	36.799549797214695
2	10.201910166442884	10.419348370278854
3	5.184556532313227	7.942586208011798
4	3.292123730144656	6.724580683971876
5	2.3446406404377678	5.98653272702575
6	1.7264965926076052	5.289882727349172
7	1.308489347148684	4.677322328376359
8	1.0564183112507284	4.315737045350168
9	0.7448129100904416	3.423093591725583
>10	2.0634357662199476	13.447867681779076
>50	0.005066754490411167	0.1778818476425804
>100	0.008866820358219542	0.7956169912740868
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.180469219971927	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	251	0.16235761366650064	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	199	0.12872177338499455	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12807493030265787	0.0
2	0.0	0.0	0.0	0.701177901252935	0.0
3	0.0	0.0	0.0	0.9450377432938544	0.0
4	0.0	0.0	0.0	1.2341766010983395	0.0
5	0.0	0.0	0.0	2.2438986526258593	0.0
6	0.0	0.0	0.0	2.8687490701630693	0.0
7	0.0	0.0	0.0	3.492952644617942	0.0
8	0.0	0.0	0.0	4.871375253077356	0.0
9	0.0	0.0	0.0	5.375266014217611	0.0
10	0.0	0.0	0.0	6.526646700776858	0.0
11	0.0	0.0	0.0	7.763410674204545	0.0
12	0.0	0.0	0.0	8.853988111024147	0.0
13	0.0	0.0	0.0	9.260205566731567	0.0
14	0.0	0.0	0.0	9.41997580806872	0.0
15	0.0	0.0	0.0	9.71817046902592	0.0
16	0.0	0.0	0.0	10.306150830869939	0.0
17	0.0	0.0	0.0	11.012503476781568	0.0
18	0.0	0.0	0.0	11.877332677865677	0.0
19	0.0	0.0	0.0	12.332063364748345	0.0
20	0.0	0.0	0.0	12.803611971771767	0.0
21	0.0	0.0	0.0	13.432343447802998	0.0
22	0.0	0.0	0.0	14.072718099316287	0.0
23	0.0	0.0	0.0	14.727323298640982	0.0
24	0.0	0.0	0.0	15.232507745945911	0.0
25	0.0	0.0	0.0	15.660717866452778	0.0
26	0.0	0.0	0.0	16.118035925664792	0.0
27	0.0	0.0	0.0	16.53848392918362	0.0
28	0.0	0.0	0.0	16.932411366326644	0.0
29	0.0	0.0	0.0	17.402019444103054	0.0
30	0.0	0.0	0.0	17.92272812538406	0.0
31	0.0	0.0	0.0	18.409800966383564	0.0
32	0.0	0.0	0.0	18.9026953951241	0.0
33	0.0	0.0	0.0	19.3315523587133	0.0
34	0.0	0.0	0.0	19.719658208115295	0.0
35	0.0	0.0	0.0	20.20543736295012	0.0
36	0.0	0.0	0.0	20.65111224668008	0.0
37	0.0	0.0	0.0	21.123307696785837	0.0
38	0.0	0.0	0.0	21.55927993428074	0.0
39	0.0	0.0	0.0	22.106509181937554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAGGA	25	3.8750997E-5	45.000004	4
GGCATAT	25	3.8750997E-5	45.000004	8
GGAATCC	40	6.7484507E-9	45.0	8
TGAACCG	20	7.0143293E-4	45.0	1
TAGGGCG	35	1.202825E-7	45.0	5
AAACACG	20	7.0143293E-4	45.0	40
CGAGACA	20	7.0143293E-4	45.0	22
CACCGGG	20	7.0143293E-4	45.0	3
GGCGATT	70	0.0	41.785713	8
AGGCGAT	55	6.002665E-11	40.909092	7
GTAAGGA	45	1.909757E-8	40.000004	4
GTAGGGT	40	3.4323966E-7	39.375	4
CGGGCAC	40	3.4323966E-7	39.375	6
CGGTAGG	40	3.4323966E-7	39.375	31
GTGTAAG	35	6.213264E-6	38.571426	1
CGTTTTA	35	6.213264E-6	38.571426	1
GACCGAT	90	0.0	37.500004	9
GGGACCG	90	0.0	37.500004	7
ATCCTGC	30	1.13535636E-4	37.499996	20
GGCACCG	30	1.13535636E-4	37.499996	8
>>END_MODULE