##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936503.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74589
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21515236831168	33.0	31.0	34.0	30.0	34.0
2	32.32724664494765	33.0	31.0	34.0	31.0	34.0
3	32.26242475432034	34.0	31.0	34.0	30.0	34.0
4	35.958143962246446	37.0	35.0	37.0	35.0	37.0
5	36.005657670702114	37.0	35.0	37.0	35.0	37.0
6	35.96683157033879	37.0	35.0	37.0	35.0	37.0
7	26.60979500998807	36.0	10.0	37.0	0.0	37.0
8	31.066873131426885	36.0	19.0	37.0	17.0	37.0
9	36.05254125943504	39.0	32.0	39.0	32.0	39.0
10	36.85774041748783	38.0	37.0	39.0	34.0	39.0
11	37.203609111263056	39.0	37.0	39.0	34.0	39.0
12	37.17688935365805	39.0	37.0	39.0	34.0	39.0
13	37.093499041413615	39.0	37.0	39.0	33.0	39.0
14	38.31208355119388	40.0	38.0	41.0	34.0	41.0
15	38.44507903310139	40.0	38.0	41.0	34.0	41.0
16	38.403196181742615	40.0	37.0	41.0	34.0	41.0
17	38.301693279169854	40.0	37.0	41.0	34.0	41.0
18	38.24738232179008	40.0	37.0	41.0	34.0	41.0
19	38.12768638807331	40.0	37.0	41.0	34.0	41.0
20	38.1683224067892	40.0	37.0	41.0	34.0	41.0
21	38.1168536915631	40.0	37.0	41.0	34.0	41.0
22	38.0868090469104	40.0	37.0	41.0	34.0	41.0
23	38.05141508801566	40.0	36.0	41.0	34.0	41.0
24	37.98043947498961	40.0	36.0	41.0	34.0	41.0
25	37.89337569882959	40.0	36.0	41.0	33.0	41.0
26	37.711713523441794	40.0	36.0	41.0	33.0	41.0
27	37.606336054914266	40.0	36.0	41.0	33.0	41.0
28	37.55433106758369	40.0	36.0	41.0	33.0	41.0
29	37.53314831945729	40.0	36.0	41.0	33.0	41.0
30	37.50337181085683	40.0	36.0	41.0	33.0	41.0
31	37.389977074367536	40.0	35.0	41.0	33.0	41.0
32	37.254139350306346	40.0	35.0	41.0	33.0	41.0
33	37.178672458405394	39.0	35.0	41.0	32.0	41.0
34	37.06188580085536	39.0	35.0	41.0	32.0	41.0
35	36.97660512944268	39.0	35.0	41.0	32.0	41.0
36	36.82089852391103	39.0	35.0	41.0	31.0	41.0
37	36.71658019278982	39.0	35.0	41.0	31.0	41.0
38	36.581747978924504	39.0	35.0	41.0	31.0	41.0
39	36.53704969901728	39.0	35.0	41.0	31.0	41.0
40	36.30491091179665	39.0	35.0	41.0	30.0	41.0
41	36.249004544906086	39.0	35.0	40.0	30.0	41.0
42	36.162919465336714	39.0	35.0	40.0	30.0	41.0
43	36.131011275121	38.0	35.0	40.0	30.0	41.0
44	35.94579629704112	38.0	35.0	40.0	30.0	41.0
45	35.859885505905694	38.0	35.0	40.0	29.0	41.0
46	35.77713872018662	38.0	35.0	40.0	29.0	41.0
47	35.66126372521418	38.0	35.0	40.0	29.0	41.0
48	35.594712357049964	38.0	35.0	40.0	28.0	41.0
49	35.43105551756962	38.0	34.0	40.0	28.0	41.0
50	35.27915644397967	37.0	34.0	40.0	28.0	41.0
51	33.210687903042	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	8.0
11	5.0
12	2.0
13	1.0
14	5.0
15	4.0
16	7.0
17	14.0
18	37.0
19	43.0
20	98.0
21	125.0
22	194.0
23	264.0
24	346.0
25	419.0
26	582.0
27	778.0
28	833.0
29	1026.0
30	1240.0
31	1668.0
32	2216.0
33	3110.0
34	5386.0
35	7226.0
36	7064.0
37	11370.0
38	14609.0
39	15892.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33883012240411	17.821662711659897	23.42972824411106	15.409778921824934
2	28.737481398061377	26.415423185724435	27.34183324618912	17.50526217002507
3	33.44729115553232	24.345412862486427	26.41006046467978	15.797235517301477
4	28.835351057126385	26.43151134885841	25.954229175883842	18.77890841813136
5	26.45162155277588	30.36774859563743	23.95661558674872	19.22401426483798
6	26.759978012843717	36.88211398463581	22.46443845607261	13.893469546447868
7	61.444717049430885	29.253643298609717	5.327863357867782	3.973776294091622
8	84.93209454477201	5.949938999048117	6.0625561409859365	3.0554103151939294
9	79.30257812814222	7.974366193406535	7.494402659909638	5.228653018541609
10	46.66639853061444	26.049417474426523	13.441660298435426	13.842523696523616
11	37.297724865596805	25.46890292134229	20.271085548807466	16.962286664253444
12	33.67520679993029	23.617423480674095	24.437919800506776	18.269449918888846
13	26.310850125353603	27.276139913392054	26.140583732185714	20.27242622906863
14	19.882288273069758	30.854415530440143	26.73986780892625	22.523428387563847
15	20.559331804957836	25.761171218276154	34.08545495984663	19.594042016919385
16	23.743447425223557	23.668369330598345	32.16023810481438	20.42794513936371
17	22.118542948692166	23.873493410556517	28.497499631312927	25.510464009438387
18	24.00219871562831	25.82016114976739	27.61265065894435	22.56498947565995
19	27.502714877528856	26.17812277949832	25.097534488999717	21.221627853973104
20	30.775315395031438	24.171124428535038	24.942015578704634	20.111544597728887
21	27.559023448497765	27.00264114011449	24.34407218222526	21.094263229162475
22	25.9595918969285	24.960785102360937	24.78917802893188	24.29044497177868
23	25.889876523347947	27.262733110780413	24.491547010953358	22.355843354918285
24	24.730188097440642	24.423172317633966	28.34466208154017	22.501977503385216
25	23.86544932898953	25.972998699540145	26.90343080078832	23.258121170682003
26	23.74612878574589	29.236214455214576	25.176634624408422	21.84102213463111
27	24.467414766252396	29.106168469881617	24.81599163415517	21.610425129710816
28	21.70427274799233	28.44655378138868	28.94930887932537	20.899864591293625
29	23.42034348228291	26.987893657241685	28.195846572550913	21.395916287924493
30	23.211197361541245	27.00130045985333	27.310997600182336	22.476504578423093
31	26.136561691402214	26.533403048706916	24.537130139832954	22.79290512005792
32	27.710520318009358	27.593881135288044	24.281060209950528	20.414538336752067
33	25.466221560819964	29.04449717786805	24.9433562589658	20.54592500234619
34	24.0518038852914	27.59119977476572	25.830886591856704	22.526109748086178
35	24.55187762270576	26.95035460992908	26.031988631031382	22.465779136333776
36	25.11228197187253	29.010980171338936	25.109600611350196	20.767137245438335
37	24.990280068106557	28.98818860689914	25.636487953987853	20.385043371006446
38	23.756854227835202	29.77784928072504	23.58390647414498	22.881390017294777
39	24.73286945796297	26.806901821984475	25.761171218276154	22.6990575017764
40	25.782622102454784	25.561409859362637	27.56572684980359	21.090241188378982
41	22.311600906299855	26.444918151470056	26.944991888884417	24.29848905334567
42	23.649599806942042	27.146093928059095	26.32693828848758	22.87736797651128
43	25.013071632546353	26.962420732279558	25.16322782179678	22.86127981337731
44	23.805118717237125	28.11540575688104	25.806754347155746	22.272721178726083
45	22.6802879781201	28.17707704889461	25.215514351982193	23.927120621003098
46	22.829103487109357	29.41854697073295	24.37088578744855	23.381463754709138
47	23.864108648728365	26.98923433750285	26.69294399978549	22.453713013983297
48	24.459370684685407	25.0680395232541	27.166204131976563	23.306385660083926
49	22.5140436257357	26.577645497325342	27.66091514834627	23.247395728592686
50	21.149231119870223	28.110043035836384	26.281355159607983	24.459370684685407
51	21.542050436391424	28.276287388220783	25.206129590154042	24.975532585233747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	77.0
2	141.0
3	232.0
4	323.0
5	231.5
6	140.0
7	137.0
8	134.0
9	130.0
10	126.0
11	135.0
12	144.0
13	149.0
14	154.0
15	146.5
16	139.0
17	141.5
18	144.0
19	148.0
20	152.0
21	173.5
22	195.0
23	242.5
24	290.0
25	383.0
26	599.0
27	722.0
28	853.5
29	985.0
30	1133.0
31	1281.0
32	1518.5
33	1756.0
34	1911.0
35	2066.0
36	2151.5
37	2237.0
38	2558.5
39	2880.0
40	3251.5
41	3623.0
42	4140.0
43	4657.0
44	4957.0
45	5257.0
46	5382.5
47	5508.0
48	5243.5
49	4979.0
50	4835.0
51	4691.0
52	4494.5
53	4298.0
54	4261.5
55	4225.0
56	4325.5
57	4426.0
58	4298.0
59	4170.0
60	4174.5
61	4179.0
62	3752.5
63	3326.0
64	2790.5
65	2255.0
66	1868.0
67	1481.0
68	1250.5
69	1020.0
70	873.0
71	726.0
72	608.0
73	490.0
74	386.5
75	252.0
76	221.0
77	175.0
78	129.0
79	94.0
80	59.0
81	49.5
82	40.0
83	29.5
84	19.0
85	19.0
86	19.0
87	11.0
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	74589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.47468125326791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.850137025818555	29.209400849991283
2	13.35352661185634	12.412017857861079
3	7.488821577960479	10.441217873949242
4	5.042550122602049	9.374036386062288
5	3.3953555459397085	7.889903336953171
6	2.3799221116399827	6.636367292764349
7	1.745276215202654	5.677780906031721
8	1.350064906966681	5.0195068977999435
9	0.8135006490696668	3.402646502835539
>10	1.563536708495601	9.18500046923809
>50	0.008654262224145393	0.27349877327756106
>100	0.008654262224145393	0.4786228532357318
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.16758503264556435	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	122	0.16356299186207082	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	110	0.14747482872809664	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	97	0.13004598533295794	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02815428548445481	0.0
2	0.0	0.0	0.0	0.18099183525720952	0.0
3	0.0	0.0	0.0	0.24132244700961267	0.0
4	0.0	0.0	0.0	0.3311480245076352	0.0
5	0.0	0.0	0.0	0.6971537358055477	0.0
6	0.0	0.0	0.0	0.856694686884125	0.0
7	0.0	0.0	0.0	1.0631594471034602	0.0
8	0.0	0.0	0.0	1.3809006689994503	0.0
9	0.0	0.0	0.0	1.5243534569440533	0.0
10	0.0	0.0	0.0	2.0351526364477337	0.0
11	0.0	0.0	0.0	2.3783667833058493	0.0
12	0.0	0.0	0.0	2.9025727654211746	0.0
13	0.0	0.0	0.0	3.0272560297094744	0.0
14	0.0	0.0	0.0	3.092949362506536	0.0
15	0.0	0.0	0.0	3.180093579482229	0.0
16	0.0	0.0	0.0	3.3892397002238934	0.0
17	0.0	0.0	0.0	3.6158146643606965	0.0
18	0.0	0.0	0.0	3.874565954765448	0.0
19	0.0	0.0	0.0	4.000589899314912	0.0
20	0.0	0.0	0.0	4.149405408304173	0.0
21	0.0	0.0	0.0	4.334419284344877	0.0
22	0.0	0.0	0.0	4.563675609004009	0.0
23	0.0	0.0	0.0	4.79561329418547	0.0
24	0.0	0.0	0.0	4.955154245264047	0.0
25	0.0	0.0	0.0	5.06911206746303	0.0
26	0.0	0.0	0.0	5.195136012012495	0.0
27	0.0	0.0	0.0	5.346632881524085	0.0
28	0.0	0.0	0.0	5.500811111558004	0.0
29	0.0	0.0	0.0	5.659011382375417	0.0
30	0.0	0.0	0.0	5.870838863639411	0.0
31	0.0	0.0	0.0	6.058534100202443	0.0
32	0.0	0.0	0.0	6.220756411803349	0.0
33	0.0	0.0	0.0	6.370912601053774	0.0
34	0.0	0.0	0.0	6.518387429781871	0.0
35	0.0	0.0	0.0	6.714126747911891	0.0
36	0.0	0.0	0.0	6.897799943691429	0.0
37	0.0	0.0	0.0	7.110968105216587	0.0
38	0.0	0.0	0.0	7.3241362667417444	0.0
39	0.0	0.0	0.0	7.51585354408827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGTAC	20	6.990873E-4	45.000004	8
CTGTACA	20	6.990873E-4	45.000004	16
CGACGTG	20	6.990873E-4	45.000004	15
TGTATAG	20	6.990873E-4	45.000004	2
AGGGTAC	20	6.990873E-4	45.000004	6
TAACGGG	35	1.19161996E-7	45.000004	3
CCTGTAC	20	6.990873E-4	45.000004	15
CAAATTG	20	6.990873E-4	45.000004	41
TATACGG	20	6.990873E-4	45.000004	2
CGTTTTA	35	1.19161996E-7	45.000004	1
GAGATTT	20	6.990873E-4	45.000004	9
CGCGGGC	25	3.8556936E-5	45.0	4
CGGGACC	25	3.8556936E-5	45.0	6
GGCGATA	50	1.0531949E-9	40.5	8
TACGGGG	40	3.4005825E-7	39.375004	4
AGGCGAT	40	3.4005825E-7	39.375004	7
GAGCTAG	35	6.1700484E-6	38.57143	45
CGGGCGA	35	6.1700484E-6	38.57143	6
GACGGGA	35	6.1700484E-6	38.57143	4
AACTTAA	130	0.0	38.07692	45
>>END_MODULE