##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936502.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183075
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08569711866721	33.0	31.0	34.0	30.0	34.0
2	32.21166734944695	33.0	31.0	34.0	30.0	34.0
3	32.14033046565615	33.0	31.0	34.0	30.0	34.0
4	35.85096272019664	37.0	35.0	37.0	35.0	37.0
5	35.90496244708453	37.0	35.0	37.0	35.0	37.0
6	35.86795029359552	37.0	35.0	37.0	35.0	37.0
7	26.672942783012427	36.0	10.0	37.0	0.0	37.0
8	31.090848013109383	36.0	19.0	37.0	17.0	37.0
9	35.979762392462106	39.0	32.0	39.0	32.0	39.0
10	36.71515499112385	38.0	35.0	39.0	33.0	39.0
11	37.05946196913833	39.0	37.0	39.0	34.0	39.0
12	37.09097364468114	39.0	37.0	39.0	34.0	39.0
13	37.04452273658337	39.0	37.0	39.0	33.0	39.0
14	38.234412126177794	40.0	37.0	41.0	33.0	41.0
15	38.34010651372388	40.0	37.0	41.0	34.0	41.0
16	38.26848013109382	40.0	37.0	41.0	34.0	41.0
17	38.19581592243616	40.0	37.0	41.0	34.0	41.0
18	38.10040147480541	40.0	37.0	41.0	33.0	41.0
19	37.99257135053939	40.0	37.0	41.0	34.0	41.0
20	38.051350539396424	40.0	37.0	41.0	34.0	41.0
21	37.96073740270381	40.0	36.0	41.0	33.0	41.0
22	37.949709135600166	40.0	36.0	41.0	33.0	41.0
23	37.90228048613956	40.0	36.0	41.0	33.0	41.0
24	37.86107879284446	40.0	36.0	41.0	33.0	41.0
25	37.74407756383996	40.0	36.0	41.0	33.0	41.0
26	37.60468660385088	40.0	36.0	41.0	33.0	41.0
27	37.477481906322545	40.0	36.0	41.0	33.0	41.0
28	37.44649460603578	40.0	36.0	41.0	33.0	41.0
29	37.44136556056261	40.0	36.0	41.0	33.0	41.0
30	37.34984569165643	40.0	36.0	41.0	32.0	41.0
31	37.216353953297826	39.0	35.0	41.0	32.0	41.0
32	37.08901816195548	39.0	35.0	41.0	32.0	41.0
33	36.989091902225866	39.0	35.0	41.0	31.0	41.0
34	36.86972552232692	39.0	35.0	41.0	31.0	41.0
35	36.784536392189	39.0	35.0	41.0	31.0	41.0
36	36.63891301379216	39.0	35.0	41.0	30.0	41.0
37	36.53663798989485	39.0	35.0	41.0	30.0	41.0
38	36.43387682643725	39.0	35.0	41.0	30.0	41.0
39	36.38942509900314	39.0	35.0	41.0	30.0	41.0
40	36.19729619008603	39.0	35.0	40.0	30.0	41.0
41	36.109993172197186	39.0	35.0	40.0	29.0	41.0
42	36.02474941963676	39.0	35.0	40.0	29.0	41.0
43	35.968908917110475	38.0	35.0	40.0	29.0	41.0
44	35.77279257135054	38.0	35.0	40.0	28.0	41.0
45	35.671549911238564	38.0	35.0	40.0	28.0	41.0
46	35.57272156220128	38.0	34.0	40.0	28.0	41.0
47	35.41917793254131	38.0	34.0	40.0	27.0	41.0
48	35.351325959306294	38.0	34.0	40.0	27.0	41.0
49	35.176452273658334	38.0	34.0	40.0	27.0	41.0
50	34.98435613819473	37.0	34.0	40.0	26.0	41.0
51	32.93273248668579	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	10.0
10	13.0
11	12.0
12	7.0
13	9.0
14	8.0
15	18.0
16	34.0
17	53.0
18	98.0
19	146.0
20	235.0
21	343.0
22	498.0
23	681.0
24	988.0
25	1325.0
26	1704.0
27	2032.0
28	2368.0
29	2821.0
30	3451.0
31	4403.0
32	5754.0
33	8286.0
34	13290.0
35	16138.0
36	17336.0
37	27838.0
38	35052.0
39	38112.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15048477399972	18.69343165369384	23.555373480813873	15.600710091492559
2	29.54253721152533	26.095316127270245	26.406663935545545	17.955482725658882
3	33.13396149119213	23.474532295507306	26.924484500887612	16.467021712412947
4	29.384678410487503	26.51645500477946	25.268332650553056	18.83053393417998
5	26.3815376211935	30.28376348491055	24.04642905912877	19.28826983476717
6	27.891301379216166	35.3685647958487	22.969274887341253	13.770858937593882
7	61.70913560016387	28.94223678820156	5.272702444353407	4.075925167281169
8	84.48340843916428	5.409258500614502	6.684145841868087	3.4231872183531338
9	78.0614502253175	8.22832172606855	8.491601802539943	5.218626246074013
10	44.41567663525877	26.269015430834358	15.344530929946743	13.970777003960125
11	34.46674860030042	26.30670490236242	22.141745186399017	17.08480131093814
12	32.14802676498702	23.60234876416769	25.140516181892668	19.109108288952616
13	26.10624061177113	25.682916837361734	27.06868769629933	21.1421548545678
14	21.562201283626926	29.06349856616141	27.080158405025262	22.294141745186398
15	21.076061723337432	26.745869179298104	31.98470572169876	20.19336337566571
16	24.07210159770586	24.394920114707087	30.581455687559746	20.95152260002731
17	24.136556056261096	24.480131093814013	27.543356547862896	23.839956302061996
18	24.936774545951113	25.805271063771677	27.15499112385634	22.102963266420865
19	27.38713641950021	26.85402157585689	24.58009012699713	21.178751877645773
20	29.481906322545402	25.756657107742733	25.06622968728663	19.695206882425236
21	27.550457462788476	26.975283353816742	24.86139560289499	20.612863580499795
22	25.629386863307385	26.05489553461696	25.287450498429603	23.028267103646048
23	25.66106786835996	27.087805544175886	25.70312713368838	21.547999453775777
24	24.740133824935135	24.373617369930358	28.221767035368018	22.66448176976649
25	24.243616004369795	25.879557558377712	26.198552505803637	23.67827393144886
26	23.610542127543358	28.311347808275293	26.02102963266421	22.057080431517136
27	24.69588966270654	27.53953297828759	25.909053666530113	21.85552369247576
28	22.63717055851427	28.345759934453092	27.9371842141199	21.07988529291274
29	23.723064317902498	26.899904410760616	27.53407073603714	21.842960535299742
30	24.556602485320226	26.152123446674857	26.712003277345346	22.579270790659567
31	26.12754335654786	26.34166325276526	25.254677044926943	22.276116345759934
32	26.224225044380717	27.670626792298236	25.253038372251808	20.852109791069235
33	25.30055987983067	27.72907278437799	25.34753516318449	21.622832172606856
34	23.40898538850198	28.170968182438894	26.70544858664482	21.71459784241431
35	24.819336337566572	26.7606172333743	25.99426464563703	22.425781783422096
36	24.879967226546498	29.03236378533388	24.470299057763214	21.61736993035641
37	24.178615321589515	29.86262460740134	25.714051618189266	20.244708452819882
38	24.18571623651509	28.764167690837088	24.590468387272978	22.459647685374843
39	24.822067458691794	26.93650143383859	25.19568482862215	23.045746278847467
40	25.21862624607401	26.232418407756384	26.647548818790114	21.90140652737949
41	22.348764167690838	27.05503209067322	27.252219035914244	23.3439847057217
42	24.539123310118804	26.970913560016385	25.67690837088625	22.81305475897856
43	24.780008193363376	26.745869179298104	25.52341936364878	22.950703263689746
44	23.62856752696982	27.381127953024713	25.873549091902227	23.116755428103236
45	23.6160043697938	26.654103509490646	25.615185033456235	24.114707087259323
46	22.28321726068551	28.3015157722245	25.563839956302065	23.85142701078793
47	23.201420182985117	26.758432336474126	27.417724976102694	22.622422504438074
48	23.838863853611908	25.168373617369934	27.54718011743821	23.445582411579956
49	22.90645910146115	26.706541035094904	26.97856069916701	23.40843916427694
50	21.04328826983477	27.020073740270377	27.867813737539258	24.06882425235559
51	21.68073194046156	26.889526150484773	26.05216441349174	25.377577495561926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	255.5
2	459.0
3	647.0
4	835.0
5	594.5
6	354.0
7	338.5
8	323.0
9	316.5
10	310.0
11	316.0
12	322.0
13	339.5
14	357.0
15	333.0
16	309.0
17	347.5
18	386.0
19	396.0
20	406.0
21	498.0
22	590.0
23	641.0
24	692.0
25	900.0
26	1412.5
27	1717.0
28	2028.5
29	2340.0
30	2760.5
31	3181.0
32	3659.5
33	4138.0
34	4636.0
35	5134.0
36	5346.5
37	5559.0
38	6327.5
39	7096.0
40	7838.0
41	8580.0
42	9440.0
43	10300.0
44	11232.5
45	12165.0
46	12653.0
47	13141.0
48	13176.5
49	13212.0
50	13386.0
51	13560.0
52	12649.5
53	11739.0
54	11217.0
55	10695.0
56	10458.0
57	10221.0
58	10111.5
59	10002.0
60	9627.0
61	9252.0
62	8407.0
63	7562.0
64	6632.5
65	5703.0
66	4600.5
67	3498.0
68	3007.0
69	2516.0
70	2169.0
71	1822.0
72	1525.5
73	1229.0
74	1052.5
75	726.0
76	576.0
77	452.0
78	328.0
79	260.0
80	192.0
81	153.5
82	115.0
83	93.5
84	72.0
85	45.5
86	19.0
87	16.5
88	14.0
89	11.5
90	9.0
91	8.0
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	183075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.89867540625427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.45478346761237	30.042878601665983
2	11.623368122909948	10.669944012016932
3	6.740530055100025	9.281442031954116
4	4.434183436670673	8.14092585006145
5	3.204845946042438	7.354909190222586
6	2.275404919730093	6.266284309709135
7	1.8124695045758012	5.823296463198143
8	1.2412381439741043	4.557694933770313
9	0.9818039010341667	4.055714870954527
>10	2.2063811303240546	12.734671582684692
>50	0.0190410453533899	0.606855114024307
>100	0.005950326672934344	0.46538303973781237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	357	0.19500204834084392	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05134507715417179	0.0
2	0.0	0.0	0.0	0.21357367199235286	0.0
3	0.0	0.0	0.0	0.28458282124812234	0.0
4	0.0	0.0	0.0	0.39273521780690973	0.0
5	0.0	0.0	0.0	0.730301788884337	0.0
6	0.0	0.0	0.0	0.9750102417042196	0.0
7	0.0	0.0	0.0	1.2033319677727707	0.0
8	0.0	0.0	0.0	1.6796394920114708	0.0
9	0.0	0.0	0.0	1.8877509217533797	0.0
10	0.0	0.0	0.0	2.371705585142701	0.0
11	0.0	0.0	0.0	2.904274204560972	0.0
12	0.0	0.0	0.0	3.3570940871227637	0.0
13	0.0	0.0	0.0	3.513314215485457	0.0
14	0.0	0.0	0.0	3.583777140516182	0.0
15	0.0	0.0	0.0	3.7208794210023215	0.0
16	0.0	0.0	0.0	4.007647139150621	0.0
17	0.0	0.0	0.0	4.313532705175475	0.0
18	0.0	0.0	0.0	4.666939778779189	0.0
19	0.0	0.0	0.0	4.893622832172607	0.0
20	0.0	0.0	0.0	5.095725795439027	0.0
21	0.0	0.0	0.0	5.338249351358733	0.0
22	0.0	0.0	0.0	5.644134917383586	0.0
23	0.0	0.0	0.0	5.938003550457463	0.0
24	0.0	0.0	0.0	6.188174245527789	0.0
25	0.0	0.0	0.0	6.415403523146251	0.0
26	0.0	0.0	0.0	6.6235149528881605	0.0
27	0.0	0.0	0.0	6.833265055305203	0.0
28	0.0	0.0	0.0	7.039737812371978	0.0
29	0.0	0.0	0.0	7.239109654513178	0.0
30	0.0	0.0	0.0	7.513314215485456	0.0
31	0.0	0.0	0.0	7.783695206882426	0.0
32	0.0	0.0	0.0	8.025126314352041	0.0
33	0.0	0.0	0.0	8.257817834220948	0.0
34	0.0	0.0	0.0	8.510173426191452	0.0
35	0.0	0.0	0.0	8.763621466612044	0.0
36	0.0	0.0	0.0	9.0449269425099	0.0
37	0.0	0.0	0.0	9.321862624607402	0.0
38	0.0	0.0	0.0	9.594974737129592	0.0
39	0.0	0.0	0.0	9.875187764577358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.0177356E-4	45.000004	9
TCAGACG	20	7.0177356E-4	45.000004	22
GCGATAC	20	7.0177356E-4	45.000004	9
AATAGGC	20	7.0177356E-4	45.000004	4
CATATGC	25	3.877922E-5	45.0	33
TTACGGG	25	3.877922E-5	45.0	3
AGGCGAT	85	0.0	42.35294	7
GGCACCG	50	1.0713848E-9	40.5	8
AAGGGCG	50	1.0713848E-9	40.5	5
AAACACG	45	1.9130312E-8	40.0	40
TCAAGCG	45	1.9130312E-8	40.0	17
TGGGCGA	40	3.4370169E-7	39.375004	6
GGTAATT	35	6.2195522E-6	38.57143	8
CGAAAAA	35	6.2195522E-6	38.57143	22
TTGCGAG	35	6.2195522E-6	38.57143	2
AATTATC	105	0.0	38.57143	45
TAAGGCG	35	6.2195522E-6	38.57143	5
ACATACG	35	6.2195522E-6	38.57143	17
GGCGATT	65	9.094947E-12	38.07692	8
TGCGGGA	95	0.0	37.894737	4
>>END_MODULE