##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936501.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227546
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09072451284575	33.0	31.0	34.0	30.0	34.0
2	32.212787744016595	33.0	31.0	34.0	30.0	34.0
3	32.0777952589806	33.0	31.0	34.0	30.0	34.0
4	35.8228929535127	37.0	35.0	37.0	35.0	37.0
5	35.896161655225754	37.0	35.0	37.0	35.0	37.0
6	35.86596995772283	37.0	35.0	37.0	35.0	37.0
7	26.72153762316191	36.0	10.0	37.0	0.0	37.0
8	31.100977384792525	36.0	19.0	37.0	17.0	37.0
9	35.99755214330289	39.0	32.0	39.0	32.0	39.0
10	36.747365367881656	38.0	35.0	39.0	33.0	39.0
11	37.094372126954546	39.0	37.0	39.0	34.0	39.0
12	37.14673516563684	39.0	37.0	39.0	34.0	39.0
13	37.13457498703559	39.0	37.0	39.0	34.0	39.0
14	38.37646453903826	40.0	38.0	41.0	34.0	41.0
15	38.44061860019512	40.0	38.0	41.0	34.0	41.0
16	38.38082849182143	40.0	38.0	41.0	34.0	41.0
17	38.28902287889042	40.0	37.0	41.0	34.0	41.0
18	38.16640591352957	40.0	37.0	41.0	34.0	41.0
19	38.03750889929948	40.0	37.0	41.0	34.0	41.0
20	38.09285155528992	40.0	37.0	41.0	34.0	41.0
21	38.05503063116908	40.0	37.0	41.0	34.0	41.0
22	38.073097307797106	40.0	37.0	41.0	34.0	41.0
23	38.01364998725533	40.0	37.0	41.0	34.0	41.0
24	37.9511483392369	40.0	36.0	41.0	33.0	41.0
25	37.841957230625894	40.0	36.0	41.0	33.0	41.0
26	37.73458992906929	40.0	36.0	41.0	33.0	41.0
27	37.61109841526549	40.0	36.0	41.0	33.0	41.0
28	37.575444086030956	40.0	36.0	41.0	33.0	41.0
29	37.532925210726624	40.0	36.0	41.0	33.0	41.0
30	37.41098503159801	40.0	36.0	41.0	33.0	41.0
31	37.28811756743691	40.0	36.0	41.0	32.0	41.0
32	37.118978140683645	39.0	35.0	41.0	32.0	41.0
33	37.01530679510956	39.0	35.0	41.0	31.0	41.0
34	36.88012094257864	39.0	35.0	41.0	31.0	41.0
35	36.65628927777241	39.0	35.0	41.0	30.0	41.0
36	36.524351120213055	39.0	35.0	41.0	30.0	41.0
37	36.4058739771299	39.0	35.0	41.0	30.0	41.0
38	36.29012155783885	39.0	35.0	41.0	30.0	41.0
39	36.21523999542949	39.0	35.0	41.0	29.0	41.0
40	36.00442987352008	39.0	35.0	41.0	28.0	41.0
41	35.93368373867262	39.0	35.0	40.0	28.0	41.0
42	35.82507273254639	39.0	35.0	40.0	28.0	41.0
43	35.745084510384714	39.0	35.0	40.0	27.0	41.0
44	35.56453640143092	38.0	35.0	40.0	27.0	41.0
45	35.435626203053445	38.0	35.0	40.0	26.0	41.0
46	35.268750054933946	38.0	34.0	40.0	26.0	41.0
47	35.11929456022079	38.0	34.0	40.0	24.0	41.0
48	35.05324637655683	38.0	34.0	40.0	24.0	41.0
49	34.9001960043244	38.0	34.0	40.0	24.0	41.0
50	34.74048763766447	38.0	34.0	40.0	24.0	41.0
51	32.67169715134522	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	21.0
10	17.0
11	16.0
12	8.0
13	9.0
14	17.0
15	22.0
16	27.0
17	50.0
18	93.0
19	158.0
20	283.0
21	431.0
22	658.0
23	1005.0
24	1449.0
25	2092.0
26	2517.0
27	2931.0
28	3288.0
29	3662.0
30	4455.0
31	5323.0
32	6960.0
33	9521.0
34	15338.0
35	18696.0
36	20972.0
37	33993.0
38	44116.0
39	49403.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.545243599096445	17.3687957599782	23.205857277209883	16.880103363715467
2	29.257380925175568	26.421910295061217	27.08683079465251	17.233877985110702
3	31.943870689882488	25.24720276339729	27.12154904942297	15.68737749729725
4	27.35007427069691	26.201735033795366	28.147715187258843	18.30047550824888
5	25.946841517759044	30.233007831383546	25.133379624339693	18.686771026517714
6	27.148796287344094	34.83998839795031	24.81520220087367	13.196013113831928
7	61.153788684485775	28.266811985268912	7.003858560466894	3.575540769778419
8	83.16516220895994	5.989118683694725	7.387077777680117	3.4586413296652103
9	77.16857250841588	7.469698434602234	9.302734392166858	6.058994664815026
10	41.084000597681346	27.85678500171394	16.79396693415837	14.265247466446345
11	31.710511281235444	25.831699963963327	24.768618213460137	17.689170541341092
12	27.280637761155983	24.710607964982906	28.390303499072715	19.618450774788396
13	24.016682341152997	26.156908932699324	30.35386251571111	19.47254621043657
14	22.124317720373025	28.265493570530793	28.894816872192873	20.71537183690331
15	21.18648536999112	26.32610549075791	33.257451240628264	19.229957898622697
16	24.72774735657845	25.4779253425681	29.87791479525019	19.91641250560326
17	25.144805885403393	25.43046241199582	28.03652887767748	21.388202824923315
18	25.387394197217265	24.77037609977763	29.076318634473907	20.7659110685312
19	25.737653045977517	26.23249804435147	27.23537218848057	20.794476721190442
20	26.3832367960764	26.55463071203185	27.786909020593637	19.275223471298112
21	25.859386673463824	27.071009817795083	27.565415344589667	19.504188164151422
22	25.40848883302717	24.642050398600723	27.78603007743489	22.163430690937215
23	23.408014203721443	27.17604352526522	28.073005018765436	21.3429372522479
24	23.150044386629517	25.234458087595474	29.634887011856943	21.980610513918066
25	22.442495143839047	27.80448788376856	28.428098054898786	21.324918917493605
26	22.714088579891538	27.484113102405665	27.68231478470287	22.11948353299992
27	22.45172404700588	27.743401334235717	28.54587643817074	21.258998180587664
28	21.98720258760866	27.067933516739473	29.906480447909434	21.038383447742433
29	23.475692826944883	25.725347841755074	29.033689891274733	21.765269440025314
30	23.305177854148173	26.124827507405097	29.313633287335307	21.256361351111426
31	24.573053360639168	26.617035676302812	27.53201550455732	21.277895458500698
32	24.538335105868704	26.925544725022633	27.658583319416735	20.87753684969193
33	23.93581957054837	26.97564448507115	27.16417779262215	21.924358151758323
34	24.191592029743436	26.662301248978228	28.34591686955605	20.80018985172229
35	22.652562558779323	26.849076670211737	28.721665069919926	21.77669570108901
36	24.73477890184842	27.7956984521811	26.816995244917514	20.652527401052975
37	22.851203712655902	27.572886361439007	28.28131454738822	21.29459537851687
38	22.882845666370756	28.070807660868574	27.25426946639361	21.792077206367065
39	23.65675511764654	26.9927838766667	27.249435279020506	22.101025726666258
40	24.096666168598876	26.88027915234722	28.02905786082814	20.993996818225767
41	22.09619153929315	27.481476272929427	27.680117426806007	22.742214760971407
42	23.042813321262514	26.18942982957292	28.40436658961265	22.363390259551917
43	23.33418297838679	26.3832367960764	27.319750731720177	22.962829493816635
44	23.012050310706407	26.83193727861619	27.543002294041646	22.613010116635756
45	22.243414518383094	26.912360577641444	27.54651806667663	23.297706837298833
46	23.313088342576886	27.627820308860624	27.380837281253022	21.678254067309467
47	21.805700825327627	27.042444165135844	29.276717674667978	21.875137334868555
48	22.453042461743998	26.58627266574671	28.53664753500391	22.424037337505386
49	22.653441501938072	25.776326544962338	29.063573958672094	22.5066579944275
50	21.719564395770526	26.816555773338134	29.10620270187127	22.357677129020068
51	21.830750705351885	26.83940829546553	27.48279468766755	23.847046311515037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	284.5
2	488.0
3	1142.5
4	1797.0
5	1230.5
6	664.0
7	609.0
8	554.0
9	555.5
10	557.0
11	517.5
12	478.0
13	497.0
14	516.0
15	515.5
16	515.0
17	498.0
18	481.0
19	579.0
20	677.0
21	764.0
22	851.0
23	988.0
24	1125.0
25	1344.0
26	1921.5
27	2280.0
28	2702.0
29	3124.0
30	3663.5
31	4203.0
32	4880.5
33	5558.0
34	6467.5
35	7377.0
36	7885.0
37	8393.0
38	9336.5
39	10280.0
40	11307.0
41	12334.0
42	13070.0
43	13806.0
44	14663.5
45	15521.0
46	17167.5
47	18814.0
48	18159.0
49	17504.0
50	17296.0
51	17088.0
52	16073.0
53	15058.0
54	14158.0
55	13258.0
56	12276.0
57	11294.0
58	10721.0
59	10148.0
60	9367.0
61	8586.0
62	7666.0
63	6746.0
64	5838.5
65	4931.0
66	4209.5
67	3488.0
68	3072.0
69	2656.0
70	2167.5
71	1679.0
72	1426.0
73	1173.0
74	985.5
75	655.5
76	513.0
77	381.0
78	249.0
79	187.5
80	126.0
81	107.5
82	89.0
83	76.0
84	63.0
85	47.0
86	31.0
87	21.5
88	12.0
89	11.5
90	11.0
91	6.5
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	227546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.68570882996745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.44493005276362	28.79767379905414
2	11.972849946580439	10.700305731555696
3	6.879435735132576	9.222373865238222
4	4.618045504589966	8.254425471265892
5	3.2066056586077605	7.164472339653619
6	2.3189900298192603	6.21754279512606
7	1.7812241659054342	5.571668510698631
8	1.275444461399547	4.559531186471586
9	0.9675785567855729	3.891324029277622
>10	2.511905902046157	13.906391451613425
>50	0.013993904746772924	0.42522273597784416
>100	0.007996516998155956	0.9214648154645929
>500	9.995646247694945E-4	0.36760326860269504
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.3616851098239477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	490	0.21534107389275134	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	439	0.1929280233447303	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	436	0.19160960860661141	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03735508424670177	0.0
2	0.0	0.0	0.0	0.22017526126585393	0.0
3	0.0	0.0	0.0	0.29049071396552784	0.0
4	0.0	0.0	0.0	0.45441361307164263	0.0
5	0.0	0.0	0.0	1.0375923988995632	0.0
6	0.0	0.0	0.0	1.305230590737697	0.0
7	0.0	0.0	0.0	1.5438636583372154	0.0
8	0.0	0.0	0.0	2.0123403619487927	0.0
9	0.0	0.0	0.0	2.207026271611015	0.0
10	0.0	0.0	0.0	3.0103803187047893	0.0
11	0.0	0.0	0.0	3.4015100243467256	0.0
12	0.0	0.0	0.0	4.13059337452647	0.0
13	0.0	0.0	0.0	4.286605785203871	0.0
14	0.0	0.0	0.0	4.373621157919717	0.0
15	0.0	0.0	0.0	4.527875682279627	0.0
16	0.0	0.0	0.0	4.723001063521222	0.0
17	0.0	0.0	0.0	4.97789457955754	0.0
18	0.0	0.0	0.0	5.230151266117621	0.0
19	0.0	0.0	0.0	5.407697784184297	0.0
20	0.0	0.0	0.0	5.572499626449158	0.0
21	0.0	0.0	0.0	5.789159115080028	0.0
22	0.0	0.0	0.0	6.041415801640108	0.0
23	0.0	0.0	0.0	6.289277772406458	0.0
24	0.0	0.0	0.0	6.482645267330562	0.0
25	0.0	0.0	0.0	6.672057518040308	0.0
26	0.0	0.0	0.0	6.839056718202034	0.0
27	0.0	0.0	0.0	7.017042707848083	0.0
28	0.0	0.0	0.0	7.196347112232252	0.0
29	0.0	0.0	0.0	7.38004623241015	0.0
30	0.0	0.0	0.0	7.617800356850922	0.0
31	0.0	0.0	0.0	7.830504601267436	0.0
32	0.0	0.0	0.0	8.064742953073225	0.0
33	0.0	0.0	0.0	8.274810368013501	0.0
34	0.0	0.0	0.0	8.473451521890079	0.0
35	0.0	0.0	0.0	8.714721418965835	0.0
36	0.0	0.0	0.0	8.937533509707928	0.0
37	0.0	0.0	0.0	9.158587714132526	0.0
38	0.0	0.0	0.0	9.406010213319504	0.0
39	0.0	0.0	0.0	9.695621984126287	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	40	6.7739165E-9	45.000004	11
TACGAAT	45	3.8198777E-10	45.000004	12
CGCTACG	20	7.0213474E-4	45.000004	22
GCGATTC	20	7.0213474E-4	45.000004	9
CGTTTTA	85	0.0	42.35294	1
ATAACGT	50	1.0732037E-9	40.500004	31
GGCGATT	95	0.0	40.263157	8
CGAATAT	45	1.9166691E-8	40.000004	14
AAGGGCG	75	0.0	39.0	5
CGTTTTT	375	0.0	39.0	1
GACACGA	35	6.2262206E-6	38.57143	25
CGGGATC	70	0.0	38.57143	6
TACAGTG	35	6.2262206E-6	38.57143	29
TGACGGG	65	9.094947E-12	38.07692	3
CAACGGA	30	1.13704584E-4	37.500004	37
CGACGCG	30	1.13704584E-4	37.500004	15
TTCGGGC	30	1.13704584E-4	37.500004	4
CGCAATC	30	1.13704584E-4	37.500004	20
GTAAGCG	30	1.13704584E-4	37.500004	1
TAAGCGG	30	1.13704584E-4	37.500004	2
>>END_MODULE