##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85225
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97309474919331	33.0	31.0	34.0	30.0	34.0
2	32.07877970079202	33.0	31.0	34.0	30.0	34.0
3	31.984992666471104	33.0	31.0	34.0	30.0	34.0
4	35.7239542387797	37.0	35.0	37.0	35.0	37.0
5	35.790812555001466	37.0	35.0	37.0	35.0	37.0
6	35.738738633030216	37.0	35.0	37.0	35.0	37.0
7	26.599108242886476	35.0	10.0	37.0	0.0	37.0
8	30.97744793194485	35.0	19.0	37.0	17.0	37.0
9	35.837371663244355	39.0	32.0	39.0	32.0	39.0
10	36.603754766793784	37.0	35.0	39.0	32.0	39.0
11	36.93729539454385	39.0	37.0	39.0	33.0	39.0
12	36.863960105602814	39.0	35.0	39.0	33.0	39.0
13	36.69107656204165	39.0	35.0	39.0	33.0	39.0
14	37.82404224112643	40.0	36.0	41.0	33.0	41.0
15	37.9072924611323	40.0	36.0	41.0	33.0	41.0
16	37.922968612496334	40.0	36.0	41.0	33.0	41.0
17	37.8566617776474	40.0	36.0	41.0	33.0	41.0
18	37.73574655324142	40.0	36.0	41.0	33.0	41.0
19	37.575230272807275	39.0	36.0	41.0	33.0	41.0
20	37.647497799941334	39.0	36.0	41.0	33.0	41.0
21	37.55645643883837	39.0	35.0	41.0	33.0	41.0
22	37.62977999413318	39.0	35.0	41.0	33.0	41.0
23	37.57010266940452	39.0	35.0	41.0	33.0	41.0
24	37.55488413024347	39.0	35.0	41.0	33.0	41.0
25	37.4747785274274	39.0	35.0	41.0	33.0	41.0
26	37.3273335288941	39.0	35.0	41.0	33.0	41.0
27	37.191152830742155	39.0	35.0	41.0	32.0	41.0
28	37.15049574655324	39.0	35.0	41.0	32.0	41.0
29	37.14715165737753	39.0	35.0	41.0	32.0	41.0
30	37.04519800528014	39.0	35.0	41.0	32.0	41.0
31	36.9163156350836	39.0	35.0	41.0	31.0	41.0
32	36.7717453798768	39.0	35.0	41.0	31.0	41.0
33	36.65404517453799	39.0	35.0	41.0	31.0	41.0
34	36.49045467879144	39.0	35.0	41.0	30.0	41.0
35	36.40215899090642	39.0	35.0	40.0	30.0	41.0
36	36.24010560281607	39.0	35.0	40.0	30.0	41.0
37	36.05784687591669	38.0	35.0	40.0	30.0	41.0
38	35.89870343209152	38.0	35.0	40.0	29.0	41.0
39	35.878826635376946	38.0	35.0	40.0	29.0	41.0
40	35.6041067761807	38.0	35.0	40.0	27.0	41.0
41	35.52249926664711	38.0	35.0	40.0	27.0	41.0
42	35.47568201818715	38.0	34.0	40.0	27.0	41.0
43	35.44871809914931	38.0	34.0	40.0	27.0	41.0
44	35.23076562041654	38.0	34.0	40.0	26.0	41.0
45	35.155142270460544	38.0	34.0	40.0	26.0	41.0
46	35.0530126136697	38.0	34.0	40.0	26.0	41.0
47	34.82983866236433	37.0	34.0	40.0	25.0	41.0
48	34.75304194778528	37.0	34.0	40.0	24.0	41.0
49	34.57568788501027	37.0	34.0	40.0	24.0	41.0
50	34.33650924024641	37.0	33.0	40.0	23.0	41.0
51	32.20672337929012	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	8.0
10	7.0
11	4.0
12	2.0
13	1.0
14	2.0
15	8.0
16	13.0
17	21.0
18	52.0
19	71.0
20	115.0
21	220.0
22	297.0
23	409.0
24	535.0
25	768.0
26	956.0
27	1131.0
28	1306.0
29	1559.0
30	1812.0
31	2437.0
32	3097.0
33	4279.0
34	6747.0
35	8500.0
36	8004.0
37	12343.0
38	14998.0
39	15515.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46230566148431	18.514520387210325	22.158990906424172	14.864183044881196
2	29.287180991493106	27.77354062775007	25.738926371369903	17.20035200938692
3	32.56086828982106	27.049574655324143	25.217952478732762	15.17160457612203
4	26.96743913171018	25.602816075095337	27.936638310354944	19.493106482839544
5	27.238486359636255	29.2414197711939	24.5057201525374	19.014373716632445
6	25.95482546201232	35.933118216485774	24.299207978879437	13.81284834262247
7	60.572601936051626	28.621883250220005	7.212672337929011	3.5928424757993542
8	81.51246699911998	6.826635376943385	7.501320035200938	4.1595775887357
9	76.11968319155179	7.01906717512467	9.302434731592843	7.558814901730712
10	44.34496919917865	23.534174244646525	17.538281020827224	14.582575535347608
11	36.95394543854503	24.828395423877968	22.127310061601644	16.09034907597536
12	30.696391903784097	24.188911704312115	26.022880610149603	19.09181578175418
13	24.57494866529774	29.186271633910238	29.183924904664128	17.0548547961279
14	21.537107656204167	31.98005280140804	27.686711645643886	18.796127896743915
15	19.789967732472867	25.518333822235263	36.503373423291286	18.188325022000587
16	23.108242886476972	25.71545907890877	30.959225579348782	20.217072455265473
17	24.590202405397477	24.200645350542683	26.867703138750365	24.341449105309476
18	26.708125550014667	23.862716339102377	28.56556174831329	20.86359636256967
19	26.746846582575536	26.562628336755644	25.371663244353183	21.318861836315637
20	29.349369316515105	25.791727779407452	26.212965679084775	18.645937224992668
21	24.90583748899971	28.605456145497214	27.359342915811087	19.12936344969199
22	25.30712819008507	24.801408037547667	26.587268993839835	23.304194778527425
23	22.14491053094749	29.52068055148137	27.36520973892637	20.969199178644764
24	23.90730419477853	24.31446171897917	29.79642123789968	21.98181284834262
25	21.520680551481373	29.093575828688767	27.727779407450864	21.657964212378996
26	21.943091815781752	28.220592549134643	27.862716339102377	21.973599295981224
27	23.138750366676444	28.157230859489584	28.197125256673512	20.506893517160456
28	20.538574361982988	26.61308301554708	31.155177471399238	21.693165151070694
29	23.1129363449692	26.313875036667646	29.61806981519507	20.955118803168084
30	23.353476092695804	25.39982399530654	30.327955412144327	20.91874449985333
31	26.3232619536521	25.513640363743033	26.225872689938402	21.937224992666472
32	27.748899970665885	26.72572601936052	25.607509533587564	19.917864476386036
33	24.941038427691407	26.405397477266064	26.60956292167791	22.04400117336462
34	22.66236432971546	27.734819595189204	28.120856556174832	21.481959518920505
35	22.704605456145497	28.57494866529774	27.758286887650335	20.962158990906424
36	27.481372836608976	26.90525080668818	26.399530654150777	19.213845702552067
37	23.190378410090936	28.133763567028453	27.663244353182755	21.012613669697856
38	22.80786154297448	29.11938985039601	25.691991786447637	22.380756820181873
39	24.186564975066002	27.660897623936638	26.08741566441772	22.065121736579645
40	24.960985626283367	26.610736286300966	26.94749193311822	21.480786154297448
41	21.189791727779408	27.83338222352596	26.5450278674098	24.431798181284833
42	24.70401877383397	26.055734819595187	26.97799941331769	22.262246993253154
43	25.86564975066002	24.708712232326196	26.69052508066882	22.73511293634497
44	22.886476972719272	26.553241419771194	27.537694338515696	23.022587268993842
45	21.93253153417424	28.0704018773834	26.08976239366383	23.90730419477853
46	24.667644470519214	27.40158404224113	25.68143150484013	22.24933998239953
47	21.74009973599296	27.27603402757407	29.160457612202993	21.823408624229977
48	23.856849515987093	25.837488999706657	27.67497799941332	22.63068348489293
49	24.156057494866527	24.254620123203285	29.42329128776767	22.166031094162513
50	21.943091815781752	27.943678498093284	28.484599589322386	21.62863009680258
51	22.23173951305368	27.06130830155471	25.446758580228806	25.260193605162804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	99.0
2	172.0
3	248.5
4	325.0
5	249.0
6	173.0
7	161.0
8	149.0
9	146.5
10	144.0
11	145.5
12	147.0
13	166.0
14	185.0
15	174.0
16	163.0
17	180.0
18	197.0
19	215.0
20	233.0
21	257.5
22	282.0
23	315.0
24	348.0
25	415.0
26	557.0
27	632.0
28	740.5
29	849.0
30	1042.0
31	1235.0
32	1470.5
33	1706.0
34	1936.0
35	2166.0
36	2481.5
37	2797.0
38	3172.5
39	3548.0
40	4015.5
41	4483.0
42	5020.0
43	5557.0
44	6201.0
45	6845.0
46	8059.0
47	9273.0
48	8294.5
49	7316.0
50	7114.0
51	6912.0
52	6306.5
53	5701.0
54	5279.5
55	4858.0
56	4541.5
57	4225.0
58	3870.5
59	3516.0
60	3194.5
61	2873.0
62	2598.0
63	2323.0
64	2036.0
65	1749.0
66	1477.0
67	1205.0
68	1018.5
69	832.0
70	721.0
71	610.0
72	485.0
73	360.0
74	303.0
75	212.0
76	178.0
77	134.0
78	90.0
79	71.5
80	53.0
81	40.5
82	28.0
83	23.5
84	19.0
85	13.0
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	85225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.77676738046348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.82367091652634	33.56996186564975
2	11.176329083473659	10.902904077442065
3	6.194370940582151	9.064241713112349
4	4.005292278085157	7.814608389557056
5	2.7399566995429394	6.682311528307422
6	1.9148424344479191	5.603989439718393
7	1.4120760163579504	4.82135523613963
8	1.1210007216742843	4.3743033147550605
9	0.8371421698340148	3.6749779994133176
>10	1.7416406062063987	10.671751246699912
>50	0.016839066634592254	0.7274860662951012
>100	0.016839066634592254	2.092109122909944
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	481	0.5643883836902317	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	443	0.5198005280140804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	397	0.46582575535347615	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.14197711938985041	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTT	119	0.13963039014373715	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	117	0.13728366089762392	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTC	105	0.12320328542094457	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	98	0.11498973305954827	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	97	0.11381636843649165	No Hit
GAATCTATCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	92	0.10794954532120857	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCT	91	0.10677618069815195	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTT	88	0.1032560868289821	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCT	86	0.10090935758286887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06922851276034027	0.0
2	0.0	0.0	0.0	0.42945145203872104	0.0
3	0.0	0.0	0.0	0.5585215605749486	0.0
4	0.0	0.0	0.0	0.8542094455852156	0.0
5	0.0	0.0	0.0	2.013493693165151	0.0
6	0.0	0.0	0.0	2.459372249926665	0.0
7	0.0	0.0	0.0	2.8407157524200644	0.0
8	0.0	0.0	0.0	3.5447345262540333	0.0
9	0.0	0.0	0.0	3.7970079202112057	0.0
10	0.0	0.0	0.0	4.966852449398651	0.0
11	0.0	0.0	0.0	5.589909064241713	0.0
12	0.0	0.0	0.0	6.758580228806101	0.0
13	0.0	0.0	0.0	7.092989146377237	0.0
14	0.0	0.0	0.0	7.2373129950132	0.0
15	0.0	0.0	0.0	7.525960692285127	0.0
16	0.0	0.0	0.0	7.872103256086829	0.0
17	0.0	0.0	0.0	8.253446758580228	0.0
18	0.0	0.0	0.0	8.822528600762688	0.0
19	0.0	0.0	0.0	9.088882370196538	0.0
20	0.0	0.0	0.0	9.36931651510707	0.0
21	0.0	0.0	0.0	9.714285714285714	0.0
22	0.0	0.0	0.0	10.04517453798768	0.0
23	0.0	0.0	0.0	10.356116163097683	0.0
24	0.0	0.0	0.0	10.594309181578176	0.0
25	0.0	0.0	0.0	10.824288647697271	0.0
26	0.0	0.0	0.0	11.042534467585803	0.0
27	0.0	0.0	0.0	11.299501320035201	0.0
28	0.0	0.0	0.0	11.501320035200939	0.0
29	0.0	0.0	0.0	11.7782340862423	0.0
30	0.0	0.0	0.0	12.117336462305662	0.0
31	0.0	0.0	0.0	12.423584628923438	0.0
32	0.0	0.0	0.0	12.719272513933705	0.0
33	0.0	0.0	0.0	12.978586095629217	0.0
34	0.0	0.0	0.0	13.291874449985333	0.0
35	0.0	0.0	0.0	13.608682898210619	0.0
36	0.0	0.0	0.0	13.846875916691111	0.0
37	0.0	0.0	0.0	14.101496039894398	0.0
38	0.0	0.0	0.0	14.424171311234966	0.0
39	0.0	0.0	0.0	14.814901730712819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTATC	20	6.9965323E-4	45.000004	3
GGGCTAT	20	6.9965323E-4	45.000004	7
CTAAGGG	25	3.8603725E-5	45.000004	3
AGATTAT	20	6.9965323E-4	45.000004	16
TCGAGGG	20	6.9965323E-4	45.000004	3
GGCGATC	20	6.9965323E-4	45.000004	8
GGGGCGA	25	3.8603725E-5	45.000004	6
TGAAACG	35	1.194312E-7	45.0	4
GGCGATA	35	1.194312E-7	45.0	8
GTCTTAC	100	0.0	42.750004	31
TTACACG	100	0.0	42.750004	34
ATGTCTT	105	0.0	40.714287	29
TACACGC	105	0.0	40.714287	35
ACACGCG	110	0.0	38.863632	36
CATGGGA	35	6.180464E-6	38.571426	4
GGACTGC	35	6.180464E-6	38.571426	8
GTGCTTG	35	6.180464E-6	38.571426	1
GGGCGAT	140	0.0	38.571426	7
CGAGGGC	35	6.180464E-6	38.571426	4
GGCGACT	35	6.180464E-6	38.571426	10
>>END_MODULE