##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116132
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.073382013570765	33.0	31.0	34.0	30.0	34.0
2	32.20094375365963	33.0	31.0	34.0	30.0	34.0
3	32.10783418868184	33.0	31.0	34.0	30.0	34.0
4	35.83208762442737	37.0	35.0	37.0	35.0	37.0
5	35.89081390142252	37.0	35.0	37.0	35.0	37.0
6	35.85315847483898	37.0	35.0	37.0	35.0	37.0
7	26.781050873144352	36.0	10.0	37.0	0.0	37.0
8	31.185857472531257	36.0	19.0	37.0	17.0	37.0
9	36.022439982089345	39.0	32.0	39.0	32.0	39.0
10	36.70904660214239	38.0	35.0	39.0	33.0	39.0
11	37.1054317500775	39.0	37.0	39.0	34.0	39.0
12	37.086229463024836	39.0	37.0	39.0	34.0	39.0
13	37.01085833362036	39.0	37.0	39.0	33.0	39.0
14	38.17217476664485	40.0	37.0	41.0	33.0	41.0
15	38.321392897737056	40.0	37.0	41.0	34.0	41.0
16	38.260401956394446	40.0	37.0	41.0	34.0	41.0
17	38.19989322495092	40.0	37.0	41.0	34.0	41.0
18	38.127888953948954	40.0	37.0	41.0	34.0	41.0
19	38.01047945441394	40.0	37.0	41.0	34.0	41.0
20	38.05921709778528	40.0	37.0	41.0	34.0	41.0
21	38.007164261357765	40.0	36.0	41.0	34.0	41.0
22	37.98965831984294	40.0	36.0	41.0	33.0	41.0
23	37.926187441876486	40.0	36.0	41.0	34.0	41.0
24	37.875047359900805	40.0	36.0	41.0	33.0	41.0
25	37.779888402851924	40.0	36.0	41.0	33.0	41.0
26	37.63659456480556	40.0	36.0	41.0	33.0	41.0
27	37.518160369235005	40.0	36.0	41.0	33.0	41.0
28	37.49975889505046	40.0	36.0	41.0	33.0	41.0
29	37.50829228808597	40.0	36.0	41.0	33.0	41.0
30	37.448601591292665	40.0	36.0	41.0	33.0	41.0
31	37.29844487307547	40.0	35.0	41.0	32.0	41.0
32	37.178279888402855	39.0	35.0	41.0	32.0	41.0
33	37.114387076774705	39.0	35.0	41.0	32.0	41.0
34	37.00176523266628	39.0	35.0	41.0	31.0	41.0
35	36.90452243998209	39.0	35.0	41.0	31.0	41.0
36	36.7621327454965	39.0	35.0	41.0	31.0	41.0
37	36.70608445561947	39.0	35.0	41.0	31.0	41.0
38	36.604131505528194	39.0	35.0	41.0	30.0	41.0
39	36.569507112596014	39.0	35.0	41.0	30.0	41.0
40	36.43767437054387	39.0	35.0	41.0	30.0	41.0
41	36.33150208383564	39.0	35.0	40.0	30.0	41.0
42	36.27091585437261	39.0	35.0	40.0	30.0	41.0
43	36.21494506251507	39.0	35.0	40.0	30.0	41.0
44	36.003409912857784	39.0	35.0	40.0	29.0	41.0
45	35.9163279716185	38.0	35.0	40.0	29.0	41.0
46	35.84319567388833	38.0	35.0	40.0	29.0	41.0
47	35.72012020803913	38.0	35.0	40.0	28.0	41.0
48	35.66538077360245	38.0	35.0	40.0	28.0	41.0
49	35.53197223848724	38.0	34.0	40.0	28.0	41.0
50	35.31401336410292	38.0	34.0	40.0	27.0	41.0
51	33.207281369476114	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	13.0
10	5.0
11	5.0
12	2.0
13	2.0
14	4.0
15	2.0
16	17.0
17	30.0
18	48.0
19	67.0
20	118.0
21	172.0
22	255.0
23	447.0
24	541.0
25	821.0
26	1087.0
27	1268.0
28	1429.0
29	1700.0
30	2201.0
31	2604.0
32	3544.0
33	4951.0
34	8334.0
35	10685.0
36	10698.0
37	17015.0
38	22629.0
39	25431.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46946578031895	19.211759032824716	24.252574656425445	16.066200530430887
2	28.403024144938517	24.711535149657287	28.525298797919607	18.360141907484586
3	32.39675541625047	24.56428891261668	26.724761478317777	16.314194192815073
4	28.18689077945786	26.357076430269004	26.897840388523402	18.558192401749732
5	25.77153583852857	30.900182550890364	24.78042227809734	18.547859332483725
6	25.820617917542105	36.64709124100162	23.902111390486688	13.630179450969587
7	61.778837874143214	28.47191127337857	5.786518788964282	3.9627320635139323
8	84.61319877380912	4.9650397823166745	6.908517893431612	3.5132435504425996
9	78.1033651362243	8.103709571866496	8.768470361312989	5.024454930596218
10	42.953707849688286	27.212137912031135	16.055867461164883	13.778286777115694
11	33.48431095649778	26.251162470292428	23.13660317569662	17.127923397513175
12	30.633244928185167	24.372266042090036	27.271553060310683	17.722935969414113
13	24.506595942548135	28.03275582957324	28.393552164778015	19.06709606310061
14	19.666414080529055	31.360004133227708	28.06978266110977	20.903799125133467
15	19.51055695243344	26.305411083938967	34.024213825646676	20.159818137980917
16	21.981882685220267	25.428822374539315	32.60169462335962	19.98760031688079
17	22.412427237970586	24.614232080735714	28.23166741294389	24.74167326834981
18	23.9184720834912	25.21096683084766	29.41910928942927	21.451451796231876
19	25.77584128405607	27.12258464505907	26.329521578892983	20.77205249199187
20	27.092446526366547	26.722178211001275	25.89381049150966	20.291564771122516
21	25.354768711466264	27.611683256983433	26.54565494437364	20.487893087176662
22	24.00199772672476	26.28043949987945	25.854200392656633	23.863362380739158
23	22.610477732235733	28.093893156063785	26.43199118244756	22.86363792925292
24	22.174766644852408	26.25977336134743	30.182034236902833	21.383425756897324
25	21.466090311025386	26.7557606861158	29.128061171770053	22.65008783108876
26	21.873385457927185	29.261529983122653	27.805428305721076	21.059656253229083
27	22.074880308614336	28.346192263975478	27.991423552509215	21.587503874900975
28	19.67674714979506	28.682017015120724	30.723659284262734	20.91757655082148
29	20.975269520890024	27.00203217028898	30.60913443323115	21.413563875589848
30	22.571728722488203	27.470464643681332	28.01467295835773	21.943133675472737
31	24.380876933145043	26.977921675334965	26.029862570178764	22.61133882134123
32	24.196603864567905	28.124031274756312	26.281300588984948	21.398064271690835
33	24.14924396376537	28.596769193676163	25.92997623394069	21.32401060861778
34	20.619639720318258	28.636379292529192	27.537629593910378	23.206351393242173
35	21.82688664623015	26.915923259738918	28.151586126132333	23.105603967898595
36	22.90841456273895	28.723349292184757	26.739399993111284	21.628836151965007
37	22.247098129714463	29.01956394447697	27.087279991733542	21.64605793407502
38	20.693693383391313	28.83356869768884	26.78331553749182	23.68942238142803
39	21.90696793304171	28.08097681948128	26.559432370061653	23.452622877415354
40	22.992801295078014	27.091585437261045	27.46185375262632	22.453759515034616
41	19.687080219061066	27.51438018806186	27.61082216787793	25.18771742499914
42	21.924189715151723	26.40529742017704	27.470464643681332	24.20004822098991
43	23.21668446250818	26.130609995522335	27.584128405607412	23.06857713636207
44	21.78038783453312	28.407329590466023	26.847036131298868	22.965246443701997
45	21.072572589811596	27.764957117762545	27.02097613061	24.141494161815867
46	21.154376054834152	29.177143250783594	26.24169049013192	23.426790204250338
47	21.28612268797575	27.185444149760617	28.332414838287466	23.196018323976165
48	22.220404367443944	25.743119898047052	28.390968897461512	23.6455068370475
49	21.11907140150863	25.784452175111078	28.961870974408434	24.13460544897186
50	20.0005166534633	27.279302862260185	28.927427410188404	23.792753074088107
51	20.114180415389384	27.515241277167362	26.86684118072538	25.503737126717873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	135.0
2	236.0
3	397.5
4	559.0
5	394.0
6	229.0
7	227.5
8	226.0
9	215.5
10	205.0
11	210.5
12	216.0
13	224.5
14	233.0
15	262.5
16	292.0
17	275.5
18	259.0
19	284.5
20	310.0
21	355.0
22	400.0
23	551.0
24	702.0
25	830.0
26	1113.0
27	1268.0
28	1534.0
29	1800.0
30	2018.0
31	2236.0
32	2533.5
33	2831.0
34	3203.0
35	3575.0
36	3872.0
37	4169.0
38	4593.0
39	5017.0
40	5514.5
41	6012.0
42	6830.0
43	7648.0
44	8338.0
45	9028.0
46	9216.0
47	9404.0
48	9745.5
49	10087.0
50	9596.5
51	9106.0
52	8363.5
53	7621.0
54	7013.0
55	6405.0
56	5976.5
57	5548.0
58	5344.0
59	5140.0
60	4715.5
61	4291.0
62	3757.0
63	3223.0
64	2643.5
65	2064.0
66	1815.5
67	1567.0
68	1322.0
69	1077.0
70	959.0
71	841.0
72	699.0
73	557.0
74	451.0
75	255.0
76	165.0
77	133.5
78	102.0
79	85.5
80	69.0
81	51.5
82	34.0
83	25.0
84	16.0
85	14.0
86	12.0
87	11.0
88	10.0
89	5.5
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	116132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.99855337030276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03150976222695	33.82444115317053
2	10.783262745373706	10.56728550270382
3	6.061191852802137	8.909688974615094
4	3.9013760258685832	7.646471256845658
5	2.6729697906964485	6.548582647332346
6	2.013953570110539	5.920848689422382
7	1.5078291127005605	5.171701167636827
8	1.0790292251726623	4.22966968621913
9	0.8822030472910039	3.8904005786518785
>10	2.049101101875121	12.51420797024076
>50	0.014059012705832733	0.4658492060758447
>100	0.0035147531764581834	0.31085316708573
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.2169944545861606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0809423759170599	0.0
2	0.0	0.0	0.0	0.34357455309475426	0.0
3	0.0	0.0	0.0	0.4537939585988358	0.0
4	0.0	0.0	0.0	0.6062067302724486	0.0
5	0.0	0.0	0.0	1.066028312609789	0.0
6	0.0	0.0	0.0	1.4147694003375468	0.0
7	0.0	0.0	0.0	1.7092618744187649	0.0
8	0.0	0.0	0.0	2.5014638514793512	0.0
9	0.0	0.0	0.0	2.8777597905831294	0.0
10	0.0	0.0	0.0	3.6492956291117005	0.0
11	0.0	0.0	0.0	4.455275031860297	0.0
12	0.0	0.0	0.0	5.058898494816243	0.0
13	0.0	0.0	0.0	5.321530671993938	0.0
14	0.0	0.0	0.0	5.44552750318603	0.0
15	0.0	0.0	0.0	5.60827334412565	0.0
16	0.0	0.0	0.0	6.02762373850446	0.0
17	0.0	0.0	0.0	6.5132779940068195	0.0
18	0.0	0.0	0.0	7.103985120380257	0.0
19	0.0	0.0	0.0	7.435504425998002	0.0
20	0.0	0.0	0.0	7.7764957117762545	0.0
21	0.0	0.0	0.0	8.170013432990046	0.0
22	0.0	0.0	0.0	8.641029173698895	0.0
23	0.0	0.0	0.0	9.097406399614233	0.0
24	0.0	0.0	0.0	9.462508180346502	0.0
25	0.0	0.0	0.0	9.820721248234767	0.0
26	0.0	0.0	0.0	10.110908276788482	0.0
27	0.0	0.0	0.0	10.389901146970688	0.0
28	0.0	0.0	0.0	10.722281541693935	0.0
29	0.0	0.0	0.0	11.028829263252161	0.0
30	0.0	0.0	0.0	11.387903420245927	0.0
31	0.0	0.0	0.0	11.740088864395688	0.0
32	0.0	0.0	0.0	12.087107773912445	0.0
33	0.0	0.0	0.0	12.427237970585196	0.0
34	0.0	0.0	0.0	12.752729652464437	0.0
35	0.0	0.0	0.0	13.111803809458202	0.0
36	0.0	0.0	0.0	13.469155788240966	0.0
37	0.0	0.0	0.0	13.79636964833121	0.0
38	0.0	0.0	0.0	14.127888953948954	0.0
39	0.0	0.0	0.0	14.474046774360211	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATA	35	1.1993507E-7	45.000004	19
CGGGTAC	20	7.0070836E-4	45.000004	6
GGCGTGC	20	7.0070836E-4	45.000004	15
TGACGGG	35	1.1993507E-7	45.000004	3
ACGGGTA	20	7.0070836E-4	45.000004	5
AACGGGA	20	7.0070836E-4	45.000004	4
ACTAGAT	35	1.1993507E-7	45.000004	18
CGGGATC	20	7.0070836E-4	45.000004	6
GACGGGC	20	7.0070836E-4	45.000004	4
ACCACGC	20	7.0070836E-4	45.000004	25
GATTGAG	20	7.0070836E-4	45.000004	1
CAACTAG	35	1.1993507E-7	45.000004	16
GATTCCG	20	7.0070836E-4	45.000004	12
ACGGTTA	20	7.0070836E-4	45.000004	34
CGAAAGG	30	2.1486212E-6	44.999996	3
TAAGGAT	30	2.1486212E-6	44.999996	5
AGGCGAT	60	3.6379788E-12	41.249996	7
CGTTTTT	115	0.0	41.08696	1
TGCATGG	50	1.0641088E-9	40.5	2
AAGGCGA	50	1.0641088E-9	40.5	6
>>END_MODULE