##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936495.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151848
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02983904957589	33.0	31.0	34.0	30.0	34.0
2	32.149583794320634	33.0	31.0	34.0	30.0	34.0
3	32.059638585954374	33.0	31.0	34.0	30.0	34.0
4	35.78377061271798	37.0	35.0	37.0	35.0	37.0
5	35.85242479321427	37.0	35.0	37.0	35.0	37.0
6	35.833208208208205	37.0	35.0	37.0	35.0	37.0
7	26.77847584426532	35.0	10.0	37.0	0.0	37.0
8	31.156505189399926	36.0	19.0	37.0	17.0	37.0
9	35.96395737843106	39.0	32.0	39.0	32.0	39.0
10	36.6309664928086	38.0	35.0	39.0	33.0	39.0
11	37.03120884041937	39.0	37.0	39.0	34.0	39.0
12	37.08922738528002	39.0	37.0	39.0	34.0	39.0
13	37.075720457299404	39.0	37.0	39.0	33.0	39.0
14	38.27001343448712	40.0	38.0	41.0	34.0	41.0
15	38.3545716769401	40.0	38.0	41.0	34.0	41.0
16	38.26873584110426	40.0	37.0	41.0	34.0	41.0
17	38.17420051630578	40.0	37.0	41.0	34.0	41.0
18	38.06711316579737	40.0	37.0	41.0	33.0	41.0
19	37.93714767398978	40.0	37.0	41.0	33.0	41.0
20	38.0358845687793	40.0	37.0	41.0	34.0	41.0
21	37.943799062220116	40.0	37.0	41.0	33.0	41.0
22	37.97714820083241	40.0	37.0	41.0	33.0	41.0
23	37.925563721616356	40.0	37.0	41.0	34.0	41.0
24	37.87063379168642	40.0	36.0	41.0	33.0	41.0
25	37.77863389705495	40.0	36.0	41.0	33.0	41.0
26	37.65684763711079	40.0	36.0	41.0	33.0	41.0
27	37.5273958168695	40.0	36.0	41.0	33.0	41.0
28	37.50077709288236	40.0	36.0	41.0	33.0	41.0
29	37.492815183604655	40.0	36.0	41.0	33.0	41.0
30	37.381960908276696	40.0	36.0	41.0	32.0	41.0
31	37.25294373320689	40.0	36.0	41.0	32.0	41.0
32	37.13075575575576	39.0	35.0	41.0	32.0	41.0
33	37.03770217586007	39.0	35.0	41.0	31.0	41.0
34	36.87804251620041	39.0	35.0	41.0	31.0	41.0
35	36.79005321110584	39.0	35.0	41.0	31.0	41.0
36	36.64258995837943	39.0	35.0	41.0	30.0	41.0
37	36.59379115958063	39.0	35.0	41.0	30.0	41.0
38	36.43355855855856	39.0	35.0	41.0	30.0	41.0
39	36.408632316527054	39.0	35.0	41.0	30.0	41.0
40	36.25777092882356	39.0	35.0	41.0	30.0	41.0
41	36.15711764395975	39.0	35.0	40.0	29.0	41.0
42	36.103346767820454	39.0	35.0	40.0	29.0	41.0
43	36.05807781465676	39.0	35.0	40.0	29.0	41.0
44	35.861486486486484	38.0	35.0	40.0	28.0	41.0
45	35.773325957536485	38.0	35.0	40.0	28.0	41.0
46	35.675109319846165	38.0	35.0	40.0	28.0	41.0
47	35.55097202465623	38.0	34.0	40.0	27.0	41.0
48	35.48474790580054	38.0	34.0	40.0	27.0	41.0
49	35.29964174701017	38.0	34.0	40.0	26.0	41.0
50	35.08755465992308	38.0	34.0	40.0	26.0	41.0
51	32.982179547969025	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	17.0
10	17.0
11	4.0
12	3.0
13	7.0
14	7.0
15	9.0
16	17.0
17	35.0
18	58.0
19	97.0
20	179.0
21	277.0
22	432.0
23	608.0
24	909.0
25	1176.0
26	1460.0
27	1641.0
28	1968.0
29	2380.0
30	2884.0
31	3614.0
32	4858.0
33	6545.0
34	10499.0
35	12767.0
36	14383.0
37	23036.0
38	29679.0
39	32268.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00813971866603	18.34729466308414	23.42210631684316	15.222459301406671
2	29.993151045782625	24.75304251620041	27.782387650808705	17.47141878720826
3	32.1703282229598	24.569964701543647	26.685237869448397	16.574469206048153
4	28.126152468257732	26.852510405141985	26.40074284811127	18.620594278489015
5	25.6210157525947	30.456114008745587	25.12841789157579	18.794452347083926
6	26.172224856435385	35.49931510457826	24.479742900795532	13.848717138190821
7	61.72422422422422	28.25259469996312	6.1146672988778255	3.908513776934829
8	84.15454928612823	5.09456825246299	7.02544649913071	3.7254359622780675
9	77.45376955903271	7.52594699963121	9.400189663347557	5.620093777988515
10	40.037405826879514	27.703361255992835	17.273194246878457	14.986038670249197
11	31.382039934671514	26.666798377324692	23.823823823823822	18.127337864179967
12	27.62170064801644	24.99934144670987	27.488014330119594	19.8909435751541
13	24.1991991991992	26.71816553395501	29.646093461882934	19.436541804962857
14	21.94497128707655	28.999394130973076	28.92563616247827	20.129998419472102
15	22.23275907486434	26.77414256361625	32.19996312101575	18.793135240503663
16	24.895290026868974	26.226226226226224	30.396185659343555	18.482298087561244
17	25.35496022338128	25.672382909225018	28.24271640061114	20.729940466782573
18	25.989805595068756	24.921632158474264	29.5084558242453	19.580106422211685
19	25.729018492176387	27.581528897318368	26.629260839787154	20.060191770718085
20	27.399109635951742	26.7833623096781	26.599625941731205	19.217902112638953
21	24.86499657552289	28.21176439597492	27.84034034034034	19.082898688161844
22	24.729993151045782	25.644065117749328	28.41723302249618	21.20870870870871
23	23.320030556872663	27.408987935303724	27.782387650808705	21.48859385701491
24	23.219930456772563	26.249275591380854	29.532822295980193	20.997971655866394
25	22.61011011011011	27.329961540487858	28.533138401559455	21.526789947842577
26	22.326273642063114	28.488356777830464	28.24008218745061	20.945287392655814
27	22.53371792845477	27.670433591486223	28.091907697170853	21.70394078288815
28	20.81489384120963	28.062272799114908	29.837073916021282	21.285759443654182
29	22.316395342711132	26.716189874084613	29.1264949159686	21.840919867235655
30	22.375665138823035	26.504794267952164	29.210131183815395	21.90940940940941
31	24.135978083346505	26.45671987777251	27.967441125335863	21.439860913545125
32	24.61145355882198	26.852510405141985	27.84626731995153	20.689768716084505
33	23.504425478109688	26.25388546441178	27.85416995943312	22.387519098045413
34	22.023997681892418	26.372425056635585	30.18215584005058	21.42142142142142
35	22.176782045203097	26.699726041831305	29.133080448869926	21.990411464095676
36	23.23441862915547	27.7250935145672	28.17488541172752	20.865602444549815
37	22.40727569674938	27.767899478425797	28.695801064222117	21.12902376060271
38	21.990411464095676	28.298693430272376	28.089273484010324	21.62162162162162
39	22.190611664295876	26.6628470575839	28.913123649965755	22.23341762815447
40	23.738870449396764	25.563721616353195	28.826853168958433	21.870554765291608
41	21.395737843106264	26.401401401401404	28.533138401559455	23.669722353932883
42	22.878799852484065	26.063563563563562	28.45872187977451	22.59891470417786
43	22.46852115273168	25.99178125493915	28.841999894631478	22.6976976976977
44	21.832358674463936	26.708945787893157	28.340182287550707	23.1185132500922
45	21.723038828301984	26.963805911174333	28.14064064064064	23.172514619883042
46	22.033875981244403	26.843949212370266	28.24271640061114	22.879458405774194
47	21.166561298140245	26.61608977398451	29.538749275591382	22.67859965228386
48	22.073389178652334	25.491280754438648	29.72050998366788	22.714820083241136
49	22.217612349191295	25.170565302144247	30.028712923449763	22.58310942521469
50	20.959775565038726	25.846240977819924	29.885148306200936	23.308835150940414
51	20.54752120541594	26.122174806385335	28.139982087350507	25.190321900848218
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	151.0
2	247.0
3	486.0
4	725.0
5	540.5
6	356.0
7	312.0
8	268.0
9	290.5
10	313.0
11	300.5
12	288.0
13	288.5
14	289.0
15	320.5
16	352.0
17	328.5
18	305.0
19	380.0
20	455.0
21	495.0
22	535.0
23	632.0
24	729.0
25	870.5
26	1240.0
27	1468.0
28	1818.5
29	2169.0
30	2623.5
31	3078.0
32	3474.5
33	3871.0
34	4281.0
35	4691.0
36	5236.5
37	5782.0
38	6383.5
39	6985.0
40	7689.5
41	8394.0
42	9124.0
43	9854.0
44	10328.5
45	10803.0
46	11603.0
47	12403.0
48	12892.5
49	13382.0
50	12998.0
51	12614.0
52	11567.5
53	10521.0
54	9686.5
55	8852.0
56	8198.0
57	7544.0
58	6891.0
59	6238.0
60	5653.0
61	5068.0
62	4578.0
63	4088.0
64	3468.5
65	2849.0
66	2412.0
67	1975.0
68	1553.0
69	1131.0
70	1006.0
71	881.0
72	687.0
73	493.0
74	418.5
75	276.0
76	208.0
77	159.0
78	110.0
79	80.5
80	51.0
81	43.5
82	36.0
83	24.0
84	12.0
85	10.0
86	8.0
87	6.5
88	5.0
89	5.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	151848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.66387440071651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.42148760330579	37.94847479058006
2	12.340071802038405	13.491122701649017
3	6.712767751728791	11.008376797850481
4	3.9900730067705945	8.724513987671882
5	2.592583668650459	7.086033401822875
6	1.733609618581789	5.685949107001739
7	1.152928703949112	4.411648490595859
8	0.7336819025130713	3.2084716295242615
9	0.48912126834204755	2.406353722143196
>10	0.8252415488037009	5.52460355091934
>50	0.0060236609401729995	0.2370791844476055
>100	0.0024094643760691997	0.2673726357936884
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	286	0.18834624097781993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0790263948158685	0.0
2	0.0	0.0	0.0	0.3259838786154576	0.0
3	0.0	0.0	0.0	0.43859649122807015	0.0
4	0.0	0.0	0.0	0.5894051946683526	0.0
5	0.0	0.0	0.0	1.081344502397134	0.0
6	0.0	0.0	0.0	1.4494757915810548	0.0
7	0.0	0.0	0.0	1.7932406090300828	0.0
8	0.0	0.0	0.0	2.4913070965702544	0.0
9	0.0	0.0	0.0	2.7823876508087033	0.0
10	0.0	0.0	0.0	3.4343554080396186	0.0
11	0.0	0.0	0.0	4.111348190295558	0.0
12	0.0	0.0	0.0	4.70931457773563	0.0
13	0.0	0.0	0.0	4.962857594436541	0.0
14	0.0	0.0	0.0	5.082055739950476	0.0
15	0.0	0.0	0.0	5.277646067119751	0.0
16	0.0	0.0	0.0	5.646435909593804	0.0
17	0.0	0.0	0.0	6.090300827142932	0.0
18	0.0	0.0	0.0	6.56643485590854	0.0
19	0.0	0.0	0.0	6.873979242400295	0.0
20	0.0	0.0	0.0	7.156498603867025	0.0
21	0.0	0.0	0.0	7.506848954217375	0.0
22	0.0	0.0	0.0	7.903956588167114	0.0
23	0.0	0.0	0.0	8.308308308308309	0.0
24	0.0	0.0	0.0	8.630999420473104	0.0
25	0.0	0.0	0.0	8.93590959380433	0.0
26	0.0	0.0	0.0	9.240161213845424	0.0
27	0.0	0.0	0.0	9.550339813497708	0.0
28	0.0	0.0	0.0	9.861835519730256	0.0
29	0.0	0.0	0.0	10.179916758864128	0.0
30	0.0	0.0	0.0	10.535535535535535	0.0
31	6.585532901322376E-4	0.0	0.0	10.885227332595754	0.0
32	6.585532901322376E-4	0.0	0.0	11.259285601390864	0.0
33	6.585532901322376E-4	0.0	0.0	11.578683947105	0.0
34	6.585532901322376E-4	0.0	0.0	11.882277013855962	0.0
35	6.585532901322376E-4	0.0	0.0	12.260286602391865	0.0
36	6.585532901322376E-4	0.0	0.0	12.598782993519835	0.0
37	6.585532901322376E-4	0.0	0.0	12.908303039881988	0.0
38	6.585532901322376E-4	0.0	0.0	13.270507349454718	0.0
39	6.585532901322376E-4	0.0	0.0	13.632053105737317	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGACG	20	7.0139323E-4	45.0	1
TACGGGA	25	3.8747727E-5	45.0	4
GCGATCT	40	6.7484507E-9	45.0	9
GCGATAC	20	7.0139323E-4	45.0	9
GTGAGCG	20	7.0139323E-4	45.0	1
AGGATAC	25	3.8747727E-5	45.0	7
CCCGGGA	25	3.8747727E-5	45.0	4
CGTTTTA	30	2.1530286E-6	44.999996	1
GGCGATC	60	3.6379788E-12	41.249996	8
TAGGGCG	55	6.002665E-11	40.909092	5
TAAGGAT	45	1.9093932E-8	39.999996	5
ACGGGAG	85	0.0	39.705883	5
TGGGCGA	85	0.0	39.705883	6
CGCATGG	40	3.431851E-7	39.375	2
GGGCGAT	255	0.0	38.82353	7
ACAACGA	35	6.212531E-6	38.57143	13
TAGGTAT	35	6.212531E-6	38.57143	6
GGGACGC	35	6.212531E-6	38.57143	7
GCCTCCC	35	6.212531E-6	38.57143	42
AACGAGC	35	6.212531E-6	38.57143	15
>>END_MODULE