##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936493.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 180728 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10280642733832 33.0 31.0 34.0 30.0 34.0 2 32.23081647558762 33.0 31.0 34.0 30.0 34.0 3 32.149877163472176 33.0 31.0 34.0 30.0 34.0 4 35.86870324465495 37.0 35.0 37.0 35.0 37.0 5 35.915281528042144 37.0 35.0 37.0 35.0 37.0 6 35.8895688548537 37.0 35.0 37.0 35.0 37.0 7 26.72562082245142 36.0 10.0 37.0 0.0 37.0 8 31.135706697357353 36.0 19.0 37.0 17.0 37.0 9 36.02140786153778 39.0 32.0 39.0 32.0 39.0 10 36.749004028152804 38.0 35.0 39.0 33.0 39.0 11 37.062685361427114 39.0 37.0 39.0 34.0 39.0 12 37.04782324819618 39.0 37.0 39.0 34.0 39.0 13 36.994937143110086 39.0 37.0 39.0 33.0 39.0 14 38.089261210216456 40.0 37.0 41.0 33.0 41.0 15 38.21698353326546 40.0 37.0 41.0 33.0 41.0 16 38.17025585410119 40.0 37.0 41.0 33.0 41.0 17 38.13077663671373 40.0 37.0 41.0 33.0 41.0 18 38.02477756628746 40.0 37.0 41.0 33.0 41.0 19 37.927111460316056 40.0 36.0 41.0 33.0 41.0 20 37.95660882652383 40.0 36.0 41.0 33.0 41.0 21 37.86120025673941 40.0 36.0 41.0 33.0 41.0 22 37.8828017794697 40.0 36.0 41.0 33.0 41.0 23 37.83661635164446 40.0 36.0 41.0 33.0 41.0 24 37.77840733035279 40.0 36.0 41.0 33.0 41.0 25 37.664772475764686 40.0 36.0 41.0 33.0 41.0 26 37.52463923686424 40.0 35.0 41.0 33.0 41.0 27 37.395406356513654 39.0 35.0 41.0 33.0 41.0 28 37.34369328493648 39.0 35.0 41.0 33.0 41.0 29 37.3212894515515 39.0 35.0 41.0 32.0 41.0 30 37.21977778761454 39.0 35.0 41.0 32.0 41.0 31 37.06735536275508 39.0 35.0 41.0 32.0 41.0 32 36.925877561860915 39.0 35.0 41.0 31.0 41.0 33 36.83153136204683 39.0 35.0 41.0 31.0 41.0 34 36.70200522331902 39.0 35.0 41.0 31.0 41.0 35 36.5935826213979 39.0 35.0 41.0 30.0 41.0 36 36.43202492142889 39.0 35.0 41.0 30.0 41.0 37 36.30097715904564 39.0 35.0 41.0 30.0 41.0 38 36.14006130760037 39.0 35.0 41.0 30.0 41.0 39 36.02784294630605 39.0 35.0 40.0 29.0 41.0 40 35.795737240494 38.0 35.0 40.0 28.0 41.0 41 35.69700323137533 38.0 35.0 40.0 27.0 41.0 42 35.58695940861405 38.0 35.0 40.0 27.0 41.0 43 35.49659709618875 38.0 35.0 40.0 27.0 41.0 44 35.30270904342437 38.0 34.0 40.0 26.0 41.0 45 35.137045726174144 38.0 34.0 40.0 26.0 41.0 46 34.99150104023726 38.0 34.0 40.0 25.0 41.0 47 34.82737041299632 38.0 34.0 40.0 24.0 41.0 48 34.75344163604976 37.0 34.0 40.0 24.0 41.0 49 34.599630383781154 37.0 34.0 40.0 24.0 41.0 50 34.388976804922315 37.0 33.0 40.0 23.0 41.0 51 32.32355805409234 35.0 30.0 39.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 12.0 9 24.0 10 26.0 11 9.0 12 13.0 13 8.0 14 12.0 15 11.0 16 24.0 17 49.0 18 78.0 19 143.0 20 235.0 21 390.0 22 562.0 23 840.0 24 1174.0 25 1567.0 26 1949.0 27 2200.0 28 2601.0 29 3048.0 30 3769.0 31 4669.0 32 6068.0 33 8751.0 34 13553.0 35 15828.0 36 17145.0 37 27014.0 38 33008.0 39 35943.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.3909300163782 19.118786242308882 23.99296179894648 15.49732194236643 2 29.57427736709309 24.915895710681244 27.465583639502455 18.044243282723208 3 31.855606214864324 24.14291089371874 27.137466247620733 16.8640166437962 4 28.281173918817228 26.166393696604846 26.419259882253993 19.133172502323934 5 25.903014474790847 30.34394227789828 25.003319906157316 18.749723341153558 6 27.59063343809482 34.60338187773892 23.65543800628569 14.150546677880572 7 61.79341330618387 28.70833517772564 5.5342835642512505 3.9639679518392277 8 84.94090567039972 4.724226461865345 6.706763755477844 3.6281041122570934 9 79.90073480589615 6.488756584480545 8.722500110663539 4.888008498959763 10 46.51907839405073 23.43079102297375 15.695409676419814 14.354720906555707 11 34.11037581337701 25.43214111814439 22.693218538355982 17.764264530122613 12 30.224425656234782 23.263135762029126 26.583041919348418 19.929396662387678 13 26.095569031915367 25.112323491655967 28.29998672037537 20.492120756053296 14 22.36676109955292 26.21951219512195 29.857022708158116 21.556703997167013 15 21.181554601389934 24.191049532999866 33.05851887919968 21.56887698641052 16 25.88752157939002 24.160063742198222 29.859789296622548 20.092625381789208 17 26.64612013633748 23.8690186357399 26.92609888893807 22.55876233898455 18 26.96538444513302 23.607852684697445 28.55008631756009 20.876676552609446 19 27.227657031561243 25.539484750564384 25.525098490549336 21.70775972732504 20 27.337767252445666 26.253817892080917 26.335708910628124 20.072705944845293 21 26.91226594661591 25.245673055641625 27.7887211721482 20.053339825594264 22 26.6782125625249 23.191204461953877 27.619959275817802 22.510623699703423 23 24.191049532999866 25.1233898455137 27.834093222964896 22.851467398521535 24 23.92269045194989 24.946328183790005 28.86603072019831 22.264950644061795 25 24.355938205480058 25.20085432251782 27.36266654862556 23.080540923376567 26 23.465650037625604 26.286463635961223 26.9537647735824 23.294121552830774 27 22.768469744588554 26.259351069009785 28.380771103536805 22.59140808286486 28 21.524611570979594 26.179120003541236 29.12111017661901 23.175158248860164 29 22.69985835067062 25.87811517861095 28.95898809260325 22.46303837811518 30 24.84949758753486 26.010358107210834 27.775441547518927 21.364702757735383 31 24.935261829932273 26.30140321366916 27.2171439953964 21.546190961002168 32 27.03233588597229 25.95391970253641 25.8266566331725 21.1870877783188 33 25.956132973307955 25.863728918595903 25.853769200123942 22.326368907972203 34 23.918263910406797 26.29033685981143 27.50652914877606 22.28487008100571 35 23.497742463813022 26.93495197202426 27.23097693771856 22.33632862644416 36 25.177061661723695 27.89661812226108 25.600349696781905 21.325970519233323 37 23.818113407994336 28.037160816254257 26.386614138373687 21.758111637377716 38 24.420123057854898 27.041742286751365 26.109401974237528 22.428732681156212 39 24.52580673719623 26.275950599796378 26.318556062148645 22.879686600858747 40 25.310411225709352 25.276658846443272 26.566995706254705 22.84593422159267 41 22.740803859944226 25.814483643928998 26.6411402771015 24.803572219025273 42 23.52651498384312 25.141649329378957 27.073281395245896 24.258554291532025 43 23.185671285025013 25.744765614625294 27.32227435704484 23.747288743304857 44 22.989796821743173 26.05241025187021 27.281882165464122 23.67591076092249 45 22.878579965472976 26.170820238147936 27.051702005223323 23.898897791155772 46 23.88561816652649 26.109955291930415 26.73907750874242 23.26534903280067 47 22.659466159089902 27.090434243725376 27.54249479881369 22.707604798371033 48 23.11982647957151 25.539484750564384 27.94752335000664 23.393165419857464 49 23.56690717542384 25.3209242618742 27.603913062724097 23.508255499977867 50 22.710371386835465 25.807843831614356 28.34314550042052 23.138639281129652 51 22.73969722455845 25.87092204860343 26.140387765039176 25.248992961798944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 64.0 1 204.0 2 344.0 3 580.5 4 817.0 5 591.0 6 365.0 7 341.0 8 317.0 9 328.0 10 339.0 11 355.0 12 371.0 13 399.0 14 427.0 15 398.0 16 369.0 17 438.5 18 508.0 19 500.5 20 493.0 21 548.0 22 603.0 23 673.0 24 743.0 25 875.0 26 1174.0 27 1341.0 28 1779.0 29 2217.0 30 2598.0 31 2979.0 32 3462.5 33 3946.0 34 4530.5 35 5115.0 36 5559.5 37 6004.0 38 6586.5 39 7169.0 40 7820.0 41 8471.0 42 9425.5 43 10380.0 44 10931.5 45 11483.0 46 12002.5 47 12522.0 48 12966.0 49 13410.0 50 13234.5 51 13059.0 52 12409.0 53 11759.0 54 11231.0 55 10703.0 56 10372.5 57 10042.0 58 9726.5 59 9411.0 60 8754.5 61 8098.0 62 7569.0 63 7040.0 64 6320.5 65 5601.0 66 4822.5 67 4044.0 68 3568.5 69 3093.0 70 2697.5 71 2302.0 72 1875.5 73 1449.0 74 1234.5 75 802.5 76 585.0 77 442.5 78 300.0 79 238.0 80 176.0 81 147.0 82 118.0 83 85.0 84 52.0 85 44.5 86 37.0 87 29.5 88 22.0 89 14.0 90 6.0 91 5.0 92 4.0 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 180728.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.9985613739985 #Duplication Level Percentage of deduplicated Percentage of total 1 66.72855825391946 30.02689123987429 2 10.49738702735936 9.447346288344916 3 6.070703965570242 8.195188349342658 4 4.2311712265600985 7.615864724890444 5 3.04826314171534 6.858372803328759 6 2.2440823854903167 6.058828737107698 7 1.733784199200738 5.461245628790226 8 1.4312941899784815 5.152494356159532 9 1.0316630802336306 4.178101898986323 >10 2.9794036274208424 16.72624053826745 >50 0.0024592683676606213 0.07027134699659157 >100 0.0012296341838303106 0.20915408791111503 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.20915408791111503 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03651896773051215 0.0 2 0.0 0.0 0.0 0.18148820326678766 0.0 3 0.0 0.0 0.0 0.2534195033420389 0.0 4 0.0 0.0 0.0 0.35080341729007125 0.0 5 0.0 0.0 0.0 0.6341020760479837 0.0 6 0.0 0.0 0.0 0.8930547563188881 0.0 7 0.0 0.0 0.0 1.1149351511663936 0.0 8 0.0 0.0 0.0 1.5963215439776903 0.0 9 0.0 0.0 0.0 1.8276083396042673 0.0 10 0.0 0.0 0.0 2.292948519321854 0.0 11 0.0 0.0 0.0 2.840733035279536 0.0 12 0.0 0.0 0.0 3.276194059581249 0.0 13 0.0 0.0 0.0 3.4554689920764905 0.0 14 0.0 0.0 0.0 3.5390199637023594 0.0 15 0.0 0.0 0.0 3.6856491523172945 0.0 16 0.0 0.0 0.0 3.949028374131291 0.0 17 0.0 0.0 0.0 4.257226329069098 0.0 18 0.0 0.0 0.0 4.621862688681333 0.0 19 0.0 0.0 0.0 4.849829578150591 0.0 20 0.0 0.0 0.0 5.086649550706033 0.0 21 0.0 0.0 0.0 5.387654375636315 0.0 22 0.0 0.0 0.0 5.673166305165774 0.0 23 0.0 0.0 0.0 5.966424682395644 0.0 24 0.0 0.0 0.0 6.2286972688238675 0.0 25 0.0 0.0 0.0 6.474370324465495 0.0 26 0.0 0.0 0.0 6.677991235447744 0.0 27 0.0 0.0 0.0 6.900978265681023 0.0 28 0.0 0.0 0.0 7.1145588951352305 0.0 29 0.0 0.0 0.0 7.362998539241291 0.0 30 0.0 0.0 0.0 7.648510468770749 0.0 31 0.0 0.0 0.0 7.890310300562171 0.0 32 0.0 0.0 0.0 8.153136204683282 0.0 33 0.0 0.0 0.0 8.403789119560887 0.0 34 0.0 0.0 0.0 8.700367402948077 0.0 35 0.0 0.0 0.0 9.011885264043203 0.0 36 0.0 0.0 0.0 9.268624673542561 0.0 37 0.0 0.0 0.0 9.560776415386659 0.0 38 0.0 0.0 0.0 9.86012128723828 0.0 39 0.0 0.0 0.0 10.349807445442876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGCG 35 1.2043347E-7 45.000004 1 ACGGGAC 20 7.017495E-4 45.000004 5 CACTAGG 20 7.017495E-4 45.000004 3 CGGGCCC 20 7.017495E-4 45.000004 6 GGACGAG 20 7.017495E-4 45.000004 8 TTACGGG 20 7.017495E-4 45.000004 3 CACCGCG 20 7.017495E-4 45.000004 12 TGCGTAG 30 2.1553224E-6 45.000004 1 TATGGGC 25 3.877723E-5 45.0 4 AGGCGAT 45 3.8016879E-10 45.0 7 CGTTTTA 50 2.1827873E-11 45.0 1 ATTGGGC 55 6.002665E-11 40.909092 4 TTGATCC 45 1.9128493E-8 40.0 17 GGACCGA 75 0.0 39.000004 8 TAACACT 35 6.2191084E-6 38.57143 29 GCGGGAT 35 6.2191084E-6 38.57143 5 GCGCGAC 30 1.1361185E-4 37.500004 9 GCGATCA 30 1.1361185E-4 37.500004 9 AAGGCGA 30 1.1361185E-4 37.500004 6 GGATGAC 90 0.0 37.5 8 >>END_MODULE