SRR2936487.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337179
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7040	2.0879117620017857	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	1583	0.4694835680751174	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	1570	0.46562804919642087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	1566	0.4644417356952835	No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1540	0.45673069793789056	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1460	0.43300442791514304	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	1422	0.4217344496543379	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1208	0.35826667734348816	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1043	0.3093312454215713	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT	1009	0.2992475806619036	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	851	0.2523881973669772	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	721	0.2138330085800124	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	696	0.20641854919790378	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC	672	0.1993006681910795	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	643	0.19069989530783352	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	604	0.17913333867174408	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	583	0.17290519279077288	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	481	0.14265419851176972	No Hit
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	411	0.1218937122418656	TruSeq Adapter, Index 27 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	371	0.11003057723049181	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	367	0.10884426372935443	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	351	0.10409900972480493	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.026186E-4	45.000004	6
ACTATCC	20	7.026186E-4	45.000004	40
TAGAATT	30	2.1609212E-6	45.000004	42
GGCCGTG	20	7.026186E-4	45.000004	9
TTCACGG	20	7.026186E-4	45.000004	2
ACTTACG	20	7.026186E-4	45.000004	25
ACGGGTA	20	7.026186E-4	45.000004	5
CCATGAC	20	7.026186E-4	45.000004	20
TATCTAG	20	7.026186E-4	45.000004	1
CTACGAA	40	6.7902874E-9	45.000004	11
AGGCAAT	20	7.026186E-4	45.000004	11
CGCTACG	20	7.026186E-4	45.000004	27
GCTTACG	20	7.026186E-4	45.000004	1
TCGAGAG	20	7.026186E-4	45.000004	1
CTTACGG	20	7.026186E-4	45.000004	2
CTTACGC	20	7.026186E-4	45.000004	26
CGGGTAT	35	1.2085184E-7	45.0	6
TTCGTAG	50	2.1827873E-11	45.0	1
CGTTAGG	35	1.2085184E-7	45.0	2
TGCGTAG	25	3.884923E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content