##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337179
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80983394576768	33.0	31.0	34.0	30.0	34.0
2	31.900960617357548	33.0	31.0	34.0	30.0	34.0
3	31.84367057260387	33.0	31.0	34.0	30.0	34.0
4	35.5938418466156	37.0	35.0	37.0	33.0	37.0
5	34.66804575611174	37.0	35.0	37.0	33.0	37.0
6	35.18485730131473	37.0	35.0	37.0	33.0	37.0
7	35.773657315550494	37.0	35.0	37.0	35.0	37.0
8	35.7543115081307	37.0	35.0	37.0	35.0	37.0
9	37.62288280112344	39.0	37.0	39.0	35.0	39.0
10	37.36590060472331	39.0	37.0	39.0	34.0	39.0
11	37.30805595840785	39.0	37.0	39.0	34.0	39.0
12	37.19654842086844	39.0	37.0	39.0	34.0	39.0
13	37.08941838014823	39.0	37.0	39.0	33.0	39.0
14	37.92728194816403	40.0	37.0	41.0	33.0	41.0
15	38.074034859822234	40.0	37.0	41.0	33.0	41.0
16	38.1578093534888	40.0	37.0	41.0	33.0	41.0
17	38.16252494965582	40.0	37.0	41.0	33.0	41.0
18	38.05583977649854	39.0	37.0	41.0	33.0	41.0
19	37.84727993143108	39.0	37.0	41.0	33.0	41.0
20	37.6818277532112	39.0	35.0	41.0	33.0	41.0
21	37.58604480112937	39.0	35.0	41.0	33.0	41.0
22	37.66648278807399	39.0	35.0	41.0	33.0	41.0
23	37.66773434881769	39.0	35.0	41.0	33.0	41.0
24	37.61452225672417	39.0	35.0	41.0	33.0	41.0
25	37.48154244481418	39.0	35.0	41.0	33.0	41.0
26	37.52294182022012	39.0	35.0	41.0	33.0	41.0
27	37.522235963686946	39.0	35.0	41.0	33.0	41.0
28	37.42333300709712	39.0	35.0	41.0	33.0	41.0
29	37.36669246898531	39.0	35.0	41.0	33.0	41.0
30	37.143754504284075	39.0	35.0	41.0	33.0	41.0
31	37.022961097814516	39.0	35.0	41.0	32.0	41.0
32	36.835235883610785	39.0	35.0	41.0	32.0	41.0
33	36.42030197610171	39.0	35.0	41.0	31.0	41.0
34	36.10422060685867	39.0	35.0	41.0	30.0	41.0
35	35.87379107239775	39.0	35.0	41.0	29.0	41.0
36	35.682563267581905	39.0	35.0	41.0	27.0	41.0
37	35.579214601146575	39.0	35.0	41.0	26.0	41.0
38	35.46691519934515	39.0	35.0	41.0	25.0	41.0
39	35.35345321031262	39.0	35.0	41.0	24.0	41.0
40	35.167261306309115	38.0	35.0	40.0	23.0	41.0
41	34.992899913695695	38.0	34.0	40.0	23.0	41.0
42	35.000424107076654	38.0	34.0	40.0	23.0	41.0
43	34.868740342666655	38.0	34.0	40.0	23.0	41.0
44	34.72185397073958	38.0	34.0	40.0	22.0	41.0
45	34.66775510930396	38.0	34.0	40.0	23.0	41.0
46	34.5179177825428	38.0	34.0	40.0	22.0	41.0
47	34.37894708745207	38.0	34.0	40.0	21.0	41.0
48	34.30557656319047	37.0	34.0	40.0	21.0	41.0
49	34.2682670035797	37.0	34.0	40.0	21.0	41.0
50	34.11354503097761	37.0	34.0	40.0	20.0	41.0
51	32.90005605331293	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	6.0
12	5.0
13	5.0
14	6.0
15	11.0
16	27.0
17	54.0
18	101.0
19	218.0
20	414.0
21	644.0
22	965.0
23	1493.0
24	2274.0
25	3853.0
26	5760.0
27	6606.0
28	6644.0
29	6706.0
30	7132.0
31	8286.0
32	10258.0
33	14008.0
34	22763.0
35	24240.0
36	25944.0
37	36828.0
38	63365.0
39	88494.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.77103556271298	19.982264613157994	22.28104359998695	19.965656224142073
2	39.068565954581985	23.739616049635355	21.634799320242365	15.557018675540291
3	28.771068186334265	22.803614697237965	33.688337648548696	14.73697946787908
4	26.28188588257276	21.93641952790654	34.65488657360037	17.12680801592033
5	22.849880923782322	25.85392328703745	32.384875689173995	18.91132010000623
6	22.940930484994617	29.36422493690295	33.349051987223405	14.345792590879029
7	66.68208874218145	6.183362546303299	20.897505479285485	6.237043232229765
8	65.42222380397355	7.155546460485381	20.338158663499208	7.084071072041853
9	59.84506745675146	7.038398002248064	23.030793732705774	10.085740808294704
10	34.834316490647396	19.715047497026802	29.425320082211528	16.025315930114274
11	26.181345813351363	20.741208675510634	33.28884657704068	19.78859893409732
12	23.831851924348786	18.053615438683902	38.01482298719671	20.0997096497706
13	22.351629253304626	19.59463667666136	40.81570916338207	17.238024906651955
14	17.40084643468306	23.51866516004852	39.651639040391004	19.428849364877408
15	16.14543017210443	22.313370642892945	43.0370811942618	18.504117990740824
16	20.44196109484873	23.02426900844952	36.709878135945594	19.823891760756158
17	19.612431379178418	23.715000044486757	34.661411297856624	22.011157278478198
18	20.570082952971568	23.075280488998427	36.81249425379398	19.54214230423603
19	19.425587002749282	25.518493144590852	34.49621714282325	20.559702709836614
20	18.701639188680197	27.945097411167364	34.32420168515833	19.029061714994114
21	19.005928601721934	26.353361271016283	36.79974138365675	17.84096874360503
22	17.346276013630742	22.689728601128778	37.198342720038916	22.765652665201568
23	17.029530308827063	25.33639402216627	36.586204953452025	21.047870715554645
24	19.57565566064316	24.033821797917426	34.80139629099084	21.589126250448576
25	20.010142980434726	25.32986929791001	32.35225206789272	22.307735653762542
26	18.22948641522752	23.919639123432955	33.76337197749563	24.087502483843892
27	19.730469572541587	24.418780529036503	34.118672871086275	21.732077027335627
28	16.485012411805005	23.85735766462324	34.04719748264275	25.610432440929003
29	20.948220381459105	24.149190785903038	31.196782717784917	23.705806114852944
30	21.66030506051682	25.420325702371738	32.07584102212771	20.843528214983735
31	20.363071247023097	25.18335958051955	31.362570029568865	23.090999142888496
32	21.55205395353803	24.499449847113848	29.79693278644281	24.15156341290531
33	20.804379869446198	25.24237867720113	29.5110312326687	24.442210220683968
34	19.009190963850063	22.852550129159884	34.13795046547976	24.000308441510295
35	18.91962429451419	25.347070843676505	32.28730140370545	23.446003458103856
36	22.987789868289543	25.972554637151184	30.1172374317499	20.922418062809367
37	18.65062770813129	26.012592717814577	32.273362220067085	23.063417353987052
38	19.54570124473944	25.6540294620958	30.701200252684774	24.099069040479982
39	21.671575038777622	23.147052455817235	30.832287894560455	24.349084610844685
40	22.463735879162105	22.303880134883844	34.8399514797778	20.392432506176245
41	20.755741015899567	23.054816581103804	32.67018408619754	23.51925831679909
42	21.8557502098292	22.166860925502476	34.05876403927884	21.918624825389482
43	22.0906402830544	22.130974942093072	32.54117249294885	23.237212281903677
44	21.185779660061865	21.798807161774604	31.13183205359765	25.883581124565886
45	20.749512870018595	20.73409079450381	30.660272436895536	27.85612389858206
46	22.71108224414925	21.84388707481783	31.30651671664012	24.1385139643928
47	18.597836757330676	22.38276998270948	35.31269740998105	23.706695849978797
48	18.784087977009246	23.60200368350342	33.087766438597896	24.52614190088944
49	21.2059469895812	21.06388594782	33.999151785846685	23.73101527675211
50	19.43448435400781	20.037428190960885	34.16078700037665	26.367300454654654
51	18.48276434772035	19.938371013615914	32.42580350496324	29.153061133700497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	469.5
2	598.0
3	5816.0
4	11034.0
5	7101.5
6	3169.0
7	2970.0
8	2771.0
9	2716.0
10	2661.0
11	2643.0
12	2625.0
13	2487.0
14	2349.0
15	2220.0
16	2091.0
17	2014.5
18	1938.0
19	1802.5
20	1667.0
21	1738.0
22	1809.0
23	1917.0
24	2025.0
25	2034.5
26	2527.5
27	3011.0
28	3215.0
29	3419.0
30	3808.5
31	4198.0
32	4689.0
33	5180.0
34	5972.5
35	6765.0
36	7543.0
37	8321.0
38	8883.5
39	9446.0
40	10595.5
41	11745.0
42	13542.5
43	15340.0
44	17351.0
45	19362.0
46	27400.5
47	35439.0
48	37493.5
49	39548.0
50	39301.5
51	39055.0
52	32792.5
53	26530.0
54	22564.0
55	18598.0
56	16588.0
57	14578.0
58	13364.5
59	12151.0
60	10224.0
61	8297.0
62	7204.5
63	6112.0
64	5449.5
65	4787.0
66	3958.0
67	3129.0
68	2543.0
69	1957.0
70	1619.5
71	1282.0
72	1050.5
73	819.0
74	663.0
75	378.5
76	250.0
77	170.0
78	90.0
79	73.5
80	57.0
81	51.5
82	46.0
83	40.0
84	34.0
85	19.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.72718812040537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00668371588179	33.235585572525146
2	10.599141615917647	9.269413187080966
3	3.9554407365181663	5.188809035545338
4	2.0408286491479863	3.5695879305122706
5	1.2900097567215194	2.820424965466012
6	0.8443654956077833	2.2153037321292515
7	0.6693875462870547	2.0489304613365418
8	0.5725325133336091	2.0028189532469773
9	0.4680103324955666	1.84182982661944
>10	3.4493227252049805	26.13407561187646
>50	0.056201193307534175	1.695096731590685
>100	0.036564631790443926	2.8095673163430583
>500	0.004739859676539027	1.4123338706877848
>1k	0.00609410529840732	3.6717719770228965
>5k	6.771228109341467E-4	2.0844508280171916
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7040	2.0879117620017857	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCC	1583	0.4694835680751174	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	1570	0.46562804919642087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	1566	0.4644417356952835	No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1540	0.45673069793789056	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1460	0.43300442791514304	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	1422	0.4217344496543379	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1208	0.35826667734348816	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1043	0.3093312454215713	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT	1009	0.2992475806619036	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	851	0.2523881973669772	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	721	0.2138330085800124	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	696	0.20641854919790378	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC	672	0.1993006681910795	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	643	0.19069989530783352	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	604	0.17913333867174408	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	583	0.17290519279077288	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGT	481	0.14265419851176972	No Hit
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	411	0.1218937122418656	TruSeq Adapter, Index 27 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	371	0.11003057723049181	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	367	0.10884426372935443	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	351	0.10409900972480493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9657837528434454E-4	0.0	0.0	0.38733135812135394	0.0
2	2.9657837528434454E-4	0.0	0.0	1.5763140646362912	0.0
3	2.9657837528434454E-4	0.0	0.0	2.349197310627293	0.0
4	2.9657837528434454E-4	0.0	0.0	3.366461137852595	0.0
5	2.9657837528434454E-4	0.0	0.0	6.149552611520884	0.0
6	2.9657837528434454E-4	0.0	0.0	8.195350244232293	0.0
7	2.9657837528434454E-4	0.0	0.0	10.110356813443305	0.0
8	2.9657837528434454E-4	0.0	0.0	12.43790390267484	0.0
9	2.9657837528434454E-4	0.0	0.0	13.597821928411912	0.0
10	2.9657837528434454E-4	0.0	0.0	15.93367321215141	0.0
11	2.9657837528434454E-4	0.0	0.0	18.59665044382954	0.0
12	2.9657837528434454E-4	0.0	0.0	20.924197533061076	0.0
13	2.9657837528434454E-4	0.0	0.0	21.761734864864064	0.0
14	2.9657837528434454E-4	0.0	0.0	22.27333256222956	0.0
15	2.9657837528434454E-4	0.0	0.0	22.908603442088623	0.0
16	2.9657837528434454E-4	0.0	0.0	23.882863404897694	0.0
17	5.931567505686891E-4	0.0	0.0	24.98494864745432	0.0
18	5.931567505686891E-4	0.0	0.0	26.190836321360464	0.0
19	8.897351258530335E-4	0.0	0.0	26.99130135625291	0.0
20	8.897351258530335E-4	0.0	0.0	27.76003250498993	0.0
21	8.897351258530335E-4	0.0	0.0	28.668452068485877	0.0
22	8.897351258530335E-4	0.0	0.0	29.580133994109953	0.0
23	8.897351258530335E-4	0.0	0.0	30.369922207492163	0.0
24	8.897351258530335E-4	0.0	0.0	31.06599165428452	0.0
25	8.897351258530335E-4	0.0	0.0	31.702152269269437	0.0
26	8.897351258530335E-4	0.0	0.0	32.28433561995261	0.0
27	8.897351258530335E-4	0.0	0.0	32.972100872237	0.0
28	8.897351258530335E-4	0.0	0.0	33.58334890369804	0.0
29	8.897351258530335E-4	0.0	0.0	34.2281102915662	0.0
30	8.897351258530335E-4	0.0	0.0	35.012856672568574	0.0
31	8.897351258530335E-4	0.0	0.0	35.621435498652055	0.0
32	8.897351258530335E-4	0.0	0.0	36.19620438995311	0.0
33	8.897351258530335E-4	0.0	0.0	36.786395356768956	0.0
34	8.897351258530335E-4	0.0	0.0	37.38518709646805	0.0
35	8.897351258530335E-4	0.0	0.0	38.05812342998823	0.0
36	8.897351258530335E-4	0.0	0.0	38.61598735389808	0.0
37	8.897351258530335E-4	0.0	0.0	39.18749388307101	0.0
38	8.897351258530335E-4	0.0	0.0	39.86517547059574	0.0
39	8.897351258530335E-4	0.0	0.0	40.82994492539571	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.026186E-4	45.000004	6
ACTATCC	20	7.026186E-4	45.000004	40
TAGAATT	30	2.1609212E-6	45.000004	42
GGCCGTG	20	7.026186E-4	45.000004	9
TTCACGG	20	7.026186E-4	45.000004	2
ACTTACG	20	7.026186E-4	45.000004	25
ACGGGTA	20	7.026186E-4	45.000004	5
CCATGAC	20	7.026186E-4	45.000004	20
TATCTAG	20	7.026186E-4	45.000004	1
CTACGAA	40	6.7902874E-9	45.000004	11
AGGCAAT	20	7.026186E-4	45.000004	11
CGCTACG	20	7.026186E-4	45.000004	27
GCTTACG	20	7.026186E-4	45.000004	1
TCGAGAG	20	7.026186E-4	45.000004	1
CTTACGG	20	7.026186E-4	45.000004	2
CTTACGC	20	7.026186E-4	45.000004	26
CGGGTAT	35	1.2085184E-7	45.0	6
TTCGTAG	50	2.1827873E-11	45.0	1
CGTTAGG	35	1.2085184E-7	45.0	2
TGCGTAG	25	3.884923E-5	45.0	1
>>END_MODULE