FastQCFastQC Report
Sat 14 Jan 2017
SRR2936484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796927
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC108901.3664990645316322No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC98461.2354958484277732No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG88051.104869078347201No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.578723019799806No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC30990.3888687420554204TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT25280.3172185156231374No Hit
GAATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT17130.21495067929684902No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTC14290.179313789092351No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTA13370.16776944437821784No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACGCACT12320.15459383356317455No Hit
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC10680.13401478429015456TruSeq Adapter, Index 16 (95% over 21bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9980.12523104374679236No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT9570.12008628142853738TruSeq Adapter, Index 13 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9300.11669826721895482No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9110.1143141090714708No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA9030.11331025300937225No Hit
GAATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT8760.1099222387997897No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8700.10916934675321578No Hit
CGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG8390.10527940451258395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG253.8897248E-545.01
GCGTAAG453.8380676E-1045.01
CGTAAGG1150.043.043482
TACGGCT11400.042.0394747
CGTTTTT24400.040.850411
ACAACGA3300.040.22727213
CACAACG3250.040.15384712
CGATGAA5200.039.37500419
GCTACGA1550.039.19354610
CGGTCTA700.038.57142631
ACGGCTG12500.038.348
CCGATGA5350.037.85046818
TCACGCG301.1396077E-437.5000041
TTAACGG601.546141E-1037.5000042
AACGAGC3700.037.09459315
AGGGATC5100.037.0588236
TGATACC10400.036.9951934
ACACGAC552.746674E-936.8181826
CTACGAA1650.036.8181811
CCCGCAC10700.036.79906533
Produced by FastQC (version 0.11.5)