##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796927
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04880873655931	33.0	31.0	34.0	30.0	34.0
2	32.084647652796306	33.0	31.0	34.0	30.0	34.0
3	32.114809763002135	33.0	31.0	34.0	30.0	34.0
4	35.79415178554623	37.0	35.0	37.0	35.0	37.0
5	34.80511138410419	37.0	35.0	37.0	33.0	37.0
6	35.28832879297602	37.0	35.0	37.0	33.0	37.0
7	35.80924225179972	37.0	35.0	37.0	35.0	37.0
8	35.761752331142	37.0	35.0	37.0	35.0	37.0
9	37.65804521618668	39.0	37.0	39.0	35.0	39.0
10	37.382610954328314	39.0	37.0	39.0	34.0	39.0
11	37.294808683856864	39.0	37.0	39.0	34.0	39.0
12	37.16515816379668	39.0	37.0	39.0	34.0	39.0
13	37.06181118220364	39.0	37.0	39.0	33.0	39.0
14	38.1243840401944	40.0	37.0	41.0	33.0	41.0
15	38.18015326372428	40.0	37.0	41.0	33.0	41.0
16	38.30207911138661	40.0	37.0	41.0	34.0	41.0
17	38.213697114039306	40.0	37.0	41.0	33.0	41.0
18	38.16354697481702	40.0	37.0	41.0	34.0	41.0
19	38.07472453562246	40.0	37.0	41.0	34.0	41.0
20	37.93939093543072	40.0	36.0	41.0	33.0	41.0
21	37.9303976399344	40.0	36.0	41.0	33.0	41.0
22	37.963938980609264	40.0	36.0	41.0	33.0	41.0
23	37.95135062559055	40.0	36.0	41.0	34.0	41.0
24	37.84872642036222	40.0	36.0	41.0	33.0	41.0
25	37.76645916125317	40.0	36.0	41.0	33.0	41.0
26	37.707118719782365	40.0	36.0	41.0	33.0	41.0
27	37.71169002932515	40.0	36.0	41.0	33.0	41.0
28	37.59224119649604	40.0	35.0	41.0	33.0	41.0
29	37.50203218111571	40.0	35.0	41.0	33.0	41.0
30	37.40437455375461	40.0	35.0	41.0	33.0	41.0
31	37.333701832162795	40.0	35.0	41.0	33.0	41.0
32	37.20447669610893	40.0	35.0	41.0	32.0	41.0
33	37.03953812582583	40.0	35.0	41.0	32.0	41.0
34	36.86868558851689	40.0	35.0	41.0	31.0	41.0
35	36.7760798667883	39.0	35.0	41.0	31.0	41.0
36	36.731541282953145	39.0	35.0	41.0	31.0	41.0
37	36.67701433130011	39.0	35.0	41.0	31.0	41.0
38	36.53698393955783	39.0	35.0	41.0	31.0	41.0
39	36.46390572787721	39.0	35.0	41.0	30.0	41.0
40	36.237739466726566	39.0	35.0	41.0	30.0	41.0
41	36.174511592655286	39.0	35.0	41.0	30.0	41.0
42	36.21736495312619	39.0	35.0	41.0	30.0	41.0
43	36.15537935093177	39.0	35.0	41.0	30.0	41.0
44	36.039789089841356	39.0	35.0	41.0	30.0	41.0
45	36.013904661280144	38.0	35.0	41.0	30.0	41.0
46	35.870456139646414	38.0	35.0	40.0	29.0	41.0
47	35.683327331110625	38.0	35.0	40.0	29.0	41.0
48	35.6614646008982	38.0	35.0	40.0	29.0	41.0
49	35.684326167892415	38.0	35.0	40.0	29.0	41.0
50	35.544596932968766	38.0	35.0	40.0	28.0	41.0
51	34.37805344780639	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	16.0
10	20.0
11	23.0
12	24.0
13	28.0
14	26.0
15	39.0
16	64.0
17	128.0
18	277.0
19	492.0
20	963.0
21	1643.0
22	2521.0
23	3529.0
24	4571.0
25	6055.0
26	7707.0
27	8945.0
28	9309.0
29	10439.0
30	12499.0
31	15996.0
32	21303.0
33	29920.0
34	51660.0
35	61434.0
36	60578.0
37	89094.0
38	149753.0
39	247682.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.4752731429604	19.2007548997587	21.634352958301072	12.689618998979832
2	32.17659835844437	28.59007161258183	22.66405831399865	16.569271714975148
3	30.248567309176373	28.265826104524006	25.72883087158548	15.756775714714147
4	26.783130700804463	23.119558002175857	31.70528793728911	18.392023359730565
5	27.42610050857858	28.50612414938884	24.257052402541262	19.81072293949132
6	24.154408120191686	36.129156120949595	25.63346454568612	14.082971213172598
7	73.74263891171964	5.965163685005026	15.316584831483937	4.975612571791394
8	73.75932801875203	9.627230599540484	11.270292009180263	5.34314937252722
9	68.03170177444106	7.203169173588045	12.336638111144433	12.42849094082645
10	41.64848223237511	22.8260555860198	19.579083153162085	15.946379028443006
11	31.484063157604147	23.546573274591022	25.451139188407467	19.51822437939736
12	25.976783318923818	20.529483879953872	30.63743605123179	22.856296749890518
13	24.970794062693322	21.941783877318752	33.76971792899475	19.317704130993178
14	20.275006368211894	27.8119576824477	30.18218732707011	21.730848622270297
15	18.082710210596453	24.080499217619682	35.39834890774186	22.438441664042003
16	22.38536277475854	24.520439136834366	29.07380475250556	24.02039333590153
17	22.85855542603024	25.311603195775774	28.985967347071938	22.84387403112205
18	23.039876927246787	24.98171099736864	29.116970563175798	22.86144151220877
19	23.094461600623394	26.75615206913557	26.2608745844977	23.888511745743337
20	24.97556237898829	26.883892753037607	28.734375921508494	19.40616894646561
21	24.31979340642242	28.223538667908105	28.530718622910257	18.925949302759225
22	22.80560201875454	22.299658563456877	30.44431924128559	24.450420176502995
23	22.982280685683882	26.117699613640898	29.870991947819565	21.029027752855654
24	23.644198276630103	25.139065435102587	27.938694510287643	23.278041777979663
25	22.44923311670956	29.291641517980942	26.33704216320943	21.92208320210007
26	20.834656122831827	25.130156212551462	28.622571452592272	25.412616212024442
27	23.061334350574143	25.07996340944654	28.747175086300253	23.11152715367907
28	18.89909615309809	26.49176147878036	30.537677855060753	24.071464513060796
29	21.831610674503434	27.220435497856137	27.778203022359637	23.169750805280785
30	22.357505769035306	25.922198645547205	31.222809617443005	20.497485967974484
31	24.19518977271444	26.41120202979696	26.093356104135008	23.300252093353592
32	24.240990705547684	27.6321419653243	25.446119908096975	22.680747421031036
33	24.494966289258617	23.83744056858407	25.178843231563242	26.488749910594066
34	21.85407195389289	26.00639707275572	27.243775151299932	24.895755822051456
35	21.100050569249127	27.627875577060383	26.74510965245248	24.526964201238005
36	22.615120330971344	29.379102477391278	24.870910384514517	23.13486680712286
37	20.613556825154625	25.829969369841905	28.13833638463749	25.418137420365984
38	22.42150159299409	28.474502683432735	24.783700389119705	24.32029533445347
39	22.54020757233724	24.253413424316154	25.088119739951086	28.11825926339552
40	23.553098338994662	24.241618115586498	28.577397929797836	23.627885615621004
41	19.905210891336345	25.506100307807365	26.745235134460245	27.843453666396044
42	20.569763604445576	26.436172949341657	29.227771176029925	23.76629227018284
43	22.13013237096999	24.0944277204813	27.140754422927067	26.634685485621645
44	22.780631099209838	23.240020729627684	28.458064540415872	25.521283630746606
45	22.176435231834283	23.222327766533198	27.068476786455975	27.53276021517655
46	25.028515786263988	24.488441224854974	26.163124100450858	24.319918888430184
47	20.14337574206922	24.920601259588395	31.44968108747727	23.48634191086511
48	20.58005312908209	26.807348728302593	27.716716838556106	24.895881304059216
49	22.121223148418864	23.446062186373407	30.428759472323065	24.003955192884668
50	21.820819221835876	23.17753068976205	29.63056842094696	25.371081667455115
51	20.772668010997243	23.36073442109503	27.640298295828853	28.226299272078876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	720.5
2	1101.0
3	4448.5
4	7796.0
5	5113.5
6	2431.0
7	2381.0
8	2331.0
9	2340.0
10	2349.0
11	2328.0
12	2307.0
13	2293.5
14	2280.0
15	2170.0
16	2060.0
17	2111.5
18	2163.0
19	2238.5
20	2314.0
21	2397.0
22	2480.0
23	2661.0
24	2842.0
25	3665.5
26	5524.0
27	6559.0
28	7294.0
29	8029.0
30	9683.5
31	11338.0
32	13175.5
33	15013.0
34	16808.5
35	18604.0
36	19942.5
37	21281.0
38	23064.0
39	24847.0
40	27635.0
41	30423.0
42	33454.5
43	36486.0
44	41138.0
45	45790.0
46	70648.0
47	95506.0
48	84260.5
49	73015.0
50	71312.0
51	69609.0
52	61426.0
53	53243.0
54	49523.5
55	45804.0
56	44146.5
57	42489.0
58	40315.0
59	38141.0
60	35544.5
61	32948.0
62	29164.0
63	25380.0
64	22587.0
65	19794.0
66	17461.0
67	15128.0
68	13150.0
69	11172.0
70	9281.0
71	7390.0
72	6389.0
73	5388.0
74	4465.5
75	2525.0
76	1507.0
77	1029.5
78	552.0
79	455.0
80	358.0
81	262.5
82	167.0
83	129.5
84	92.0
85	57.5
86	23.0
87	16.5
88	10.0
89	6.0
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	796927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.319908752673637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10407504163905	23.522527772580887
2	8.997536878335403	5.636040680565617
3	3.1696610505696374	2.978204846422341
4	1.6231164295960716	2.0334343387965754
5	1.0835838621247196	1.6968873843807957
6	0.845803517283426	1.589429339040439
7	0.706331736475101	1.5485571874862378
8	0.6088207656348485	1.5254568661133086
9	0.5308103508059648	1.4962438576995751
>10	7.181369915607973	46.51063137245024
>50	0.10047079293192514	2.062818183461222
>100	0.03913922435501227	2.2657763315126185
>500	0.004841965899589147	1.2501009242747962
>1k	0.0032279772663927645	2.150648759533814
>5k	8.069943165981911E-4	2.35701903949143
>10k+	4.0349715829909557E-4	1.3762231161901064
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	10890	1.3664990645316322	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	9846	1.2354958484277732	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	8805	1.104869078347201	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4612	0.578723019799806	No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	3099	0.3888687420554204	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	2528	0.3172185156231374	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	1713	0.21495067929684902	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTC	1429	0.179313789092351	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTA	1337	0.16776944437821784	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACGCACT	1232	0.15459383356317455	No Hit
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	1068	0.13401478429015456	TruSeq Adapter, Index 16 (95% over 21bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	998	0.12523104374679236	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	957	0.12008628142853738	TruSeq Adapter, Index 13 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	930	0.11669826721895482	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	911	0.1143141090714708	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	903	0.11331025300937225	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	876	0.1099222387997897	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	870	0.10916934675321578	No Hit
CGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG	839	0.10527940451258395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.76446023286951E-4	0.0	0.0	0.1269877918554648	0.0
2	3.76446023286951E-4	0.0	0.0	0.7761062180099306	0.0
3	3.76446023286951E-4	0.0	0.0	1.100853654098807	0.0
4	3.76446023286951E-4	0.0	0.0	1.7817190282171391	0.0
5	3.76446023286951E-4	0.0	0.0	4.073020489957048	0.0
6	3.76446023286951E-4	0.0	0.0	4.976239981830205	0.0
7	5.01928031049268E-4	0.0	0.0	5.814961721713532	0.0
8	5.01928031049268E-4	0.0	0.0	6.842533883279146	0.0
9	5.01928031049268E-4	0.0	0.0	7.253738422716259	0.0
10	5.01928031049268E-4	0.0	0.0	9.117648166017716	0.0
11	5.01928031049268E-4	0.0	0.0	10.060645454351528	0.0
12	5.01928031049268E-4	0.0	0.0	12.137372682817874	0.0
13	5.01928031049268E-4	0.0	0.0	12.544185351983305	0.0
14	5.01928031049268E-4	0.0	0.0	12.75662639112491	0.0
15	5.01928031049268E-4	0.0	0.0	13.193931188176583	0.0
16	5.01928031049268E-4	0.0	0.0	13.683060054434096	0.0
17	5.01928031049268E-4	0.0	0.0	14.191764113902527	0.0
18	5.01928031049268E-4	0.0	0.0	14.754299954700995	0.0
19	5.01928031049268E-4	0.0	0.0	15.302781810630083	0.0
20	5.01928031049268E-4	0.0	0.0	15.697171761027045	0.0
21	5.01928031049268E-4	0.0	0.0	16.142758370591032	0.0
22	5.01928031049268E-4	0.0	0.0	16.67542949354207	0.0
23	5.01928031049268E-4	0.0	0.0	17.142724490448938	0.0
24	5.01928031049268E-4	0.0	0.0	17.563967590507033	0.0
25	6.27410038811585E-4	0.0	0.0	17.915066248225997	0.0
26	6.27410038811585E-4	0.0	0.0	18.26440815783629	0.0
27	6.27410038811585E-4	0.0	0.0	18.668083776807663	0.0
28	6.27410038811585E-4	0.0	0.0	19.025582016922503	0.0
29	6.27410038811585E-4	0.0	0.0	19.404286716349176	0.0
30	6.27410038811585E-4	0.0	0.0	19.910104689639077	0.0
31	7.52892046573902E-4	0.0	0.0	20.31202356050178	0.0
32	7.52892046573902E-4	0.0	0.0	20.720091049744834	0.0
33	7.52892046573902E-4	0.0	0.0	21.14447119999699	0.0
34	7.52892046573902E-4	0.0	0.0	21.574247076582925	0.0
35	7.52892046573902E-4	0.0	0.0	22.014437959813133	0.0
36	7.52892046573902E-4	0.0	0.0	22.39452296132519	0.0
37	7.52892046573902E-4	0.0	0.0	22.79054417782306	0.0
38	7.52892046573902E-4	0.0	0.0	23.229103794952362	0.0
39	7.52892046573902E-4	0.0	0.0	23.821880799621546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	25	3.8897248E-5	45.0	1
GCGTAAG	45	3.8380676E-10	45.0	1
CGTAAGG	115	0.0	43.04348	2
TACGGCT	1140	0.0	42.039474	7
CGTTTTT	2440	0.0	40.85041	1
ACAACGA	330	0.0	40.227272	13
CACAACG	325	0.0	40.153847	12
CGATGAA	520	0.0	39.375004	19
GCTACGA	155	0.0	39.193546	10
CGGTCTA	70	0.0	38.571426	31
ACGGCTG	1250	0.0	38.34	8
CCGATGA	535	0.0	37.850468	18
TCACGCG	30	1.1396077E-4	37.500004	1
TTAACGG	60	1.546141E-10	37.500004	2
AACGAGC	370	0.0	37.094593	15
AGGGATC	510	0.0	37.058823	6
TGATACC	1040	0.0	36.995193	4
ACACGAC	55	2.746674E-9	36.81818	26
CTACGAA	165	0.0	36.81818	11
CCCGCAC	1070	0.0	36.799065	33
>>END_MODULE