##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284444
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.979029264108224	33.0	31.0	34.0	30.0	34.0
2	32.01732151143986	33.0	31.0	34.0	30.0	34.0
3	32.045249680077625	33.0	31.0	34.0	30.0	34.0
4	35.71888315450493	37.0	35.0	37.0	33.0	37.0
5	34.84258061340721	37.0	35.0	37.0	33.0	37.0
6	35.311692283894196	37.0	35.0	37.0	33.0	37.0
7	35.85189703421412	37.0	35.0	37.0	35.0	37.0
8	35.83586575916525	37.0	35.0	37.0	35.0	37.0
9	37.68791748112106	39.0	37.0	39.0	35.0	39.0
10	37.391592018112526	39.0	37.0	39.0	34.0	39.0
11	37.28045590696235	39.0	37.0	39.0	34.0	39.0
12	37.04410358453685	39.0	35.0	39.0	34.0	39.0
13	36.842584129037704	39.0	35.0	39.0	33.0	39.0
14	37.8612767363699	40.0	36.0	41.0	33.0	41.0
15	38.011569939953034	40.0	36.0	41.0	33.0	41.0
16	38.05932626457229	40.0	36.0	41.0	34.0	41.0
17	38.044982492160145	40.0	36.0	41.0	34.0	41.0
18	38.04384694351085	40.0	36.0	41.0	34.0	41.0
19	37.95430383484974	40.0	36.0	41.0	34.0	41.0
20	37.94770851204455	40.0	36.0	41.0	34.0	41.0
21	37.831260283219194	40.0	35.0	41.0	34.0	41.0
22	37.88617091589206	40.0	35.0	41.0	34.0	41.0
23	37.91911588924358	40.0	35.0	41.0	34.0	41.0
24	37.89089240764439	40.0	35.0	41.0	34.0	41.0
25	37.74204764382444	40.0	35.0	41.0	34.0	41.0
26	37.7670965110883	40.0	35.0	41.0	34.0	41.0
27	37.76078243872256	40.0	35.0	41.0	34.0	41.0
28	37.666774479335125	40.0	35.0	41.0	33.0	41.0
29	37.62439355373993	40.0	35.0	41.0	33.0	41.0
30	37.53934342084909	40.0	35.0	41.0	33.0	41.0
31	37.4879519342999	40.0	35.0	41.0	33.0	41.0
32	37.42694519835187	40.0	35.0	41.0	33.0	41.0
33	37.27138909592046	40.0	35.0	41.0	33.0	41.0
34	37.15225844102881	39.0	35.0	41.0	33.0	41.0
35	37.09430327234886	39.0	35.0	41.0	32.0	41.0
36	37.03467466355416	39.0	35.0	41.0	32.0	41.0
37	36.95960540563345	39.0	35.0	41.0	32.0	41.0
38	36.87970215578462	39.0	35.0	41.0	32.0	41.0
39	36.86043298505154	39.0	35.0	41.0	32.0	41.0
40	36.72719762062128	39.0	35.0	41.0	31.0	41.0
41	36.65510258609779	39.0	35.0	41.0	31.0	41.0
42	36.67325027070355	39.0	35.0	41.0	31.0	41.0
43	36.530564891507645	39.0	35.0	41.0	31.0	41.0
44	36.39854593522802	39.0	35.0	41.0	31.0	41.0
45	36.40398461560096	39.0	35.0	41.0	31.0	41.0
46	36.28000239062874	39.0	35.0	41.0	31.0	41.0
47	36.176625275976996	38.0	35.0	40.0	30.0	41.0
48	36.1682791691862	38.0	35.0	40.0	30.0	41.0
49	36.14617991590612	38.0	35.0	40.0	30.0	41.0
50	35.98000309375483	38.0	35.0	40.0	30.0	41.0
51	34.78078989185921	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	5.0
10	3.0
11	8.0
12	4.0
13	9.0
14	6.0
15	12.0
16	16.0
17	51.0
18	59.0
19	129.0
20	233.0
21	422.0
22	564.0
23	960.0
24	1279.0
25	1778.0
26	2181.0
27	2619.0
28	2860.0
29	3460.0
30	4041.0
31	5433.0
32	7070.0
33	10293.0
34	20707.0
35	28855.0
36	19320.0
37	28670.0
38	51572.0
39	91761.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28842232565989	22.166050259453527	25.78644654132272	11.759080873563866
2	31.065165726821448	26.390080297000463	25.415899087342325	17.128854888835765
3	29.41106157978372	26.037462558535246	27.898637341620848	16.652838520060186
4	27.950317109870483	26.08597825934103	28.046293822334096	17.917410808454388
5	25.29847702887036	29.289772327769263	26.35316617682215	19.05858446653823
6	25.58746185540915	33.461419470967925	26.1851190392485	14.76599963437443
7	74.7637496308588	7.031964112443926	12.44462881973253	5.759657436964745
8	74.2139050217266	7.348370856829464	11.503846099759532	6.933878021684409
9	68.56006806260635	8.333098957967122	14.348694294834836	8.758138684591694
10	41.73580739969906	22.790777798090307	20.835032554738365	14.638382247472261
11	33.002629691608895	24.359452124143942	24.527499261717598	18.11041892252957
12	28.84188100293907	21.19327530199266	30.684774507460165	19.280069187608106
13	23.05409852202894	27.644457256964465	30.668602607191573	18.63284161381502
14	18.04327038010997	31.458916342056785	30.453797584058723	20.044015693774522
15	16.82510441422565	25.963282755129303	38.8572091518893	18.354403678755748
16	18.559013373458395	25.370899017029714	34.15610805641884	21.913979553093053
17	17.983153098676716	25.655313523927383	29.740124593944678	26.621408783451223
18	20.700383906849854	26.281798877810747	31.284189506546106	21.733627708793296
19	21.73538552403988	28.13453614771273	28.515630493172644	21.614447835074742
20	24.138318966123386	27.473246051946955	29.370280266062913	19.018154715866746
21	21.895346711479235	27.871566986823414	30.822938785841853	19.410147515855495
22	18.78331059892281	26.847463824162226	29.490163265880103	24.87906231103486
23	19.55217898777967	26.919534249272264	29.996062493847646	23.53222426910042
24	22.327066136040838	24.60941345220852	29.683522943004597	23.379997468746044
25	18.441239751937115	27.477464808538766	29.05106101728284	25.030234422241282
26	16.80963564005569	28.818677841684128	31.029657858840405	23.342028659419782
27	19.786671541674284	27.69051201642502	31.99821405970947	20.524602382191222
28	17.456863213848774	26.8246122259566	33.07751262111347	22.641011939081153
29	18.435966306197354	24.269803546568042	33.30040359438062	23.99382655285399
30	20.31928956138994	24.66179634655679	33.90403735005836	21.11487674199491
31	22.311597361870877	25.762540253969146	30.04176569025889	21.884096693901085
32	22.47191011235955	25.694688585450915	28.49453671021361	23.338864591975923
33	23.174684647944762	24.6579291530143	30.316336431775674	21.851049767265263
34	20.116789244983195	23.91648268200419	32.76813713771428	23.198590935298334
35	18.541083657943215	23.038278184809663	31.88360450563204	26.537033651615083
36	22.99644218194091	23.890467016354712	32.237276933245205	20.87581386845917
37	21.47452574144647	24.512382050596955	33.41958346809917	20.593508739857405
38	20.79354811491893	25.59976656213525	27.43387099042342	26.172814332522393
39	22.246909760796502	22.7844496632026	31.500752344925537	23.467888231075364
40	21.21120501750784	21.74065896977964	33.64774788710607	23.400388125606447
41	18.278114497053902	24.096834526303947	30.23301598908748	27.39203498755467
42	22.588277481683566	22.121753315239555	30.99872031050048	24.291248892576395
43	24.62242128503326	21.81413564708695	30.01117970496829	23.552263362911503
44	20.629368170887766	23.79730280828564	29.995359367749014	25.57796965307758
45	19.72936676463557	23.018942217097212	29.381881846690383	27.869809171576833
46	22.474371053704772	23.02456722588629	31.18856435713181	23.312497363277128
47	18.596279056686026	22.686012009393764	34.58360872438863	24.134100209531578
48	20.2064378225591	20.819563780568405	32.2288394200616	26.745158976810902
49	21.649955703055788	19.236475369492766	34.38708497982028	24.726483947631166
50	20.029250045703193	20.278508247669137	33.22446597572809	26.46777573089958
51	18.285497321089565	21.710072984489038	29.497897652965083	30.506532041456314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	520.0
2	601.0
3	1165.0
4	1729.0
5	1223.0
6	717.0
7	701.5
8	686.0
9	757.5
10	829.0
11	819.5
12	810.0
13	824.5
14	839.0
15	837.0
16	835.0
17	839.5
18	844.0
19	808.5
20	773.0
21	972.5
22	1172.0
23	1183.5
24	1195.0
25	1408.5
26	2115.0
27	2608.0
28	3020.0
29	3432.0
30	3976.5
31	4521.0
32	5261.5
33	6002.0
34	6602.5
35	7203.0
36	7520.0
37	7837.0
38	8900.0
39	9963.0
40	11846.5
41	13730.0
42	15921.0
43	18112.0
44	20612.0
45	23112.0
46	27795.5
47	32479.0
48	33183.5
49	33888.0
50	32574.0
51	31260.0
52	26000.0
53	20740.0
54	17454.5
55	14169.0
56	11936.5
57	9704.0
58	9208.5
59	8713.0
60	8349.5
61	7986.0
62	7213.5
63	6441.0
64	5087.0
65	3733.0
66	2943.0
67	2153.0
68	1828.0
69	1503.0
70	1264.0
71	1025.0
72	697.0
73	369.0
74	368.5
75	241.5
76	115.0
77	116.5
78	118.0
79	77.5
80	37.0
81	24.5
82	12.0
83	10.5
84	9.0
85	7.0
86	5.0
87	3.0
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	284444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.200756563682134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.731851730022	24.874843554443054
2	11.856252955325754	8.109856421650658
3	4.474620176394399	4.591061861034158
4	2.1545609670853807	2.947504605475946
5	1.2653933924055838	2.1638705685477633
6	0.8017927263008573	1.645315070804798
7	0.5920930901914023	1.4175022148472107
8	0.4019243025431221	1.0996892182644036
9	0.35361115108653196	1.088439200686251
>10	5.065685327193108	40.150609610327514
>50	0.23745399971217698	4.992546863354474
>100	0.054480787812750564	3.1412158456497585
>500	0.004111757570773627	0.928829576296213
>1k	0.00616763635616044	2.8487153886177947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	1877	0.6598838435685056	No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	1704	0.5990634360366188	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC	1227	0.43136786151228357	No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	1131	0.39761780877782626	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG	1095	0.38496153900240465	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC	1069	0.3758208997201558	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	916	0.32203175317461435	No Hit
TCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	675	0.23730505828915358	No Hit
ACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	546	0.19195342492722645	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC	505	0.17753933990521858	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	389	0.1367580261844159	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	347	0.1219923781130908	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	291	0.10230484735132399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4176569025889103	0.0
2	0.0	0.0	0.0	2.19129248639451	0.0
3	0.0	0.0	0.0	2.9478561685252633	0.0
4	0.0	0.0	0.0	3.8598107184542476	0.0
5	0.0	0.0	0.0	6.369970890579516	0.0
6	0.0	0.0	0.0	7.567043073504802	0.0
7	0.0	0.0	0.0	8.843920068625108	0.0
8	0.0	0.0	0.0	10.671344798976248	0.0
9	0.0	0.0	0.0	11.318572372769333	0.0
10	0.0	0.0	0.0	13.017676590119672	0.0
11	0.0	0.0	0.0	15.194203428442856	0.0
12	0.0	0.0	0.0	17.278972310894236	0.0
13	0.0	0.0	0.0	18.04959851499768	0.0
14	0.0	0.0	0.0	18.36987245292571	0.0
15	0.0	0.0	0.0	18.96084993882803	0.0
16	0.0	0.0	0.0	20.021515658618217	0.0
17	0.0	0.0	0.0	21.27905668602607	0.0
18	0.0	0.0	0.0	22.784098100153283	0.0
19	0.0	0.0	0.0	23.67495886712323	0.0
20	0.0	0.0	0.0	24.521171126829884	0.0
21	0.0	0.0	0.0	25.452110081422003	0.0
22	0.0	0.0	0.0	26.405549071170423	0.0
23	0.0	0.0	0.0	27.287269198858123	0.0
24	0.0	0.0	0.0	28.048051637580684	0.0
25	0.0	0.0	0.0	28.690708891732644	0.0
26	0.0	0.0	0.0	29.289069201670628	0.0
27	3.5156304931726457E-4	0.0	0.0	29.92891395142805	0.0
28	3.5156304931726457E-4	0.0	0.0	30.534657085401697	0.0
29	3.5156304931726457E-4	0.0	0.0	31.12422831910675	0.0
30	3.5156304931726457E-4	0.0	0.0	31.839307561418064	0.0
31	3.5156304931726457E-4	0.0	0.0	32.496027337542714	0.0
32	7.031260986345291E-4	0.0	0.0	33.12778613716584	0.0
33	7.031260986345291E-4	0.0	0.0	33.759896499838284	0.0
34	7.031260986345291E-4	0.0	0.0	34.34770991829675	0.0
35	7.031260986345291E-4	0.0	0.0	34.956968682763566	0.0
36	7.031260986345291E-4	0.0	0.0	35.5563836818495	0.0
37	7.031260986345291E-4	0.0	0.0	36.17372839645062	0.0
38	0.0010546891479517938	0.0	0.0	36.767518386747476	0.0
39	0.004921882690441704	0.0	0.0	37.381347470855424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	2.1597207E-6	45.000004	4
AACGGGC	30	2.1597207E-6	45.000004	4
TGCGACG	30	2.1597207E-6	45.000004	1
CGTAAGG	30	2.1597207E-6	45.000004	2
TAAGCGG	30	2.1597207E-6	45.000004	2
CTTCGTT	20	7.0243253E-4	45.0	23
GTGTTCA	50	2.1827873E-11	45.0	11
CTTCGAT	35	1.207627E-7	45.0	42
CGGGTAT	55	1.8189894E-12	45.0	6
TACTTCG	20	7.0243253E-4	45.0	21
TTGTGCG	40	6.7848305E-9	45.0	1
GAGTCGG	20	7.0243253E-4	45.0	2
GCGAACG	20	7.0243253E-4	45.0	1
TAGCGGA	25	3.8833787E-5	45.0	1
GGCCGGT	35	1.207627E-7	45.0	9
TTGTCGC	20	7.0243253E-4	45.0	38
CGCGCCG	20	7.0243253E-4	45.0	15
ACTTCGT	20	7.0243253E-4	45.0	22
ACTTCGC	20	7.0243253E-4	45.0	43
AGGCGTT	20	7.0243253E-4	45.0	20
>>END_MODULE