##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388916
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90173199354102	33.0	31.0	34.0	30.0	34.0
2	31.975326291538533	33.0	31.0	34.0	30.0	34.0
3	32.026188174310136	33.0	31.0	34.0	30.0	34.0
4	35.68843143506567	37.0	35.0	37.0	33.0	37.0
5	34.675917164631954	37.0	35.0	37.0	33.0	37.0
6	35.15373756800955	37.0	35.0	37.0	33.0	37.0
7	35.68473912104413	37.0	35.0	37.0	33.0	37.0
8	35.60271626778019	37.0	35.0	37.0	33.0	37.0
9	37.491180614837134	39.0	37.0	39.0	35.0	39.0
10	37.24811784549877	39.0	37.0	39.0	34.0	39.0
11	37.11262586265415	39.0	37.0	39.0	34.0	39.0
12	36.77169620175051	39.0	35.0	39.0	33.0	39.0
13	36.47330271832478	39.0	35.0	39.0	32.0	39.0
14	37.27569192319164	40.0	36.0	41.0	31.0	41.0
15	37.34880539756657	40.0	35.0	41.0	32.0	41.0
16	37.68628701313394	40.0	36.0	41.0	33.0	41.0
17	37.583655596581266	39.0	35.0	41.0	33.0	41.0
18	37.66091649610713	39.0	36.0	41.0	33.0	41.0
19	37.60033271966183	39.0	36.0	41.0	33.0	41.0
20	37.362661860144605	39.0	35.0	41.0	32.0	41.0
21	37.44910726223657	39.0	35.0	41.0	33.0	41.0
22	37.53180892532063	39.0	35.0	41.0	33.0	41.0
23	37.5138924600685	39.0	35.0	41.0	33.0	41.0
24	37.50649240452951	39.0	35.0	41.0	33.0	41.0
25	37.43780147898261	39.0	35.0	41.0	33.0	41.0
26	37.34152361949624	39.0	35.0	41.0	33.0	41.0
27	37.29460346193008	39.0	35.0	41.0	33.0	41.0
28	37.1499064065248	39.0	35.0	41.0	32.0	41.0
29	37.10481440722418	39.0	35.0	41.0	32.0	41.0
30	37.0049162286972	39.0	35.0	41.0	32.0	41.0
31	36.91296063931543	39.0	35.0	41.0	31.0	41.0
32	36.749321190179884	39.0	35.0	41.0	31.0	41.0
33	36.60342079009349	39.0	35.0	41.0	31.0	41.0
34	36.48409939421366	39.0	35.0	41.0	31.0	41.0
35	36.44614004052289	39.0	35.0	41.0	31.0	41.0
36	36.31322959199416	39.0	35.0	41.0	30.0	41.0
37	36.254862232461505	39.0	35.0	41.0	30.0	41.0
38	36.04480916187557	38.0	35.0	41.0	30.0	41.0
39	36.0399237881702	38.0	35.0	41.0	30.0	41.0
40	35.848309146448074	38.0	35.0	40.0	29.0	41.0
41	35.7302939452221	38.0	35.0	40.0	28.0	41.0
42	35.85135864813996	38.0	35.0	40.0	29.0	41.0
43	35.855333799586546	38.0	35.0	40.0	30.0	41.0
44	35.83795215419268	38.0	35.0	40.0	29.0	41.0
45	35.80157669008218	38.0	35.0	40.0	29.0	41.0
46	35.71267831614025	38.0	35.0	40.0	29.0	41.0
47	35.43314494646659	37.0	34.0	40.0	28.0	41.0
48	35.39606238879347	37.0	34.0	40.0	28.0	41.0
49	35.46468389060877	37.0	34.0	40.0	28.0	41.0
50	35.31719188719415	37.0	34.0	40.0	28.0	41.0
51	34.099610198603294	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	11.0
10	10.0
11	7.0
12	5.0
13	12.0
14	2.0
15	12.0
16	16.0
17	67.0
18	135.0
19	328.0
20	688.0
21	1164.0
22	1693.0
23	1998.0
24	2569.0
25	3055.0
26	3625.0
27	3946.0
28	4594.0
29	5471.0
30	7108.0
31	9190.0
32	12735.0
33	18246.0
34	32342.0
35	37328.0
36	28279.0
37	40497.0
38	67428.0
39	106278.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.55774511719754	18.53227946394594	22.315358586429976	9.594616832426539
2	27.97339271205093	33.89189439364799	21.967468553620833	16.167244340680252
3	27.89574098262864	33.22568369519382	23.976128521325943	14.9024468008516
4	25.76494667228913	21.45938968826173	35.337707885507406	17.437955753941726
5	31.3453290684878	27.340608254738815	22.57325489308745	18.740807783685938
6	23.548786884571477	38.162482386942166	24.117804358781843	14.170926369704512
7	70.24344588548685	5.784282467165147	18.832858509292492	5.139413138055518
8	69.61297555256148	13.84052083226198	10.508181715331846	6.038321899844696
9	64.17092636970452	6.831809439570498	11.903084470682614	17.094179720042373
10	46.66534675868311	18.92310936037602	18.960392475495993	15.451151405444877
11	37.63743327608019	20.627590533688508	22.76841271637063	18.966563473860678
12	29.711043001573607	18.198274177457343	28.7164837651318	23.37419905583725
13	27.071912700943134	22.64884962305485	33.49103662487529	16.788201051126723
14	19.937467216571186	30.39910931923603	28.934011457486964	20.729412006705818
15	16.42411214761028	22.805695831490606	40.60799761388063	20.162194407018482
16	21.7861954766582	22.661962994579806	28.468615330816938	27.08322619794506
17	21.890073949130404	24.420440403583292	28.826790360900556	24.86269528638575
18	23.208867724650055	22.79746783367102	29.383208713449694	24.610455728229233
19	22.983626284339035	25.460510752964648	26.426786246901646	25.129076715794668
20	24.25948019623775	26.629914943072542	31.183854611278527	17.92675024941118
21	21.854847833465325	30.93932880107787	28.839646607493645	18.36617675796316
22	19.898641351860043	22.012465416696664	32.71529070544796	25.37360252599533
23	20.212848018595274	27.422116858139034	30.218607617068983	22.146427506196712
24	23.07670550967304	25.25789630665748	27.177848172870235	24.487550010799247
25	21.28480185952751	31.312417077209474	25.03651174032439	22.366269322938628
26	19.49084120992708	24.29367781217538	28.504098571413877	27.711382406483665
27	24.152773349515062	26.13674932376143	26.5134373489391	23.197039977784407
28	17.60971520842547	25.390572771498217	32.60755535899783	24.392156661078484
29	24.756502689526787	24.22476833043639	27.72860977691841	23.29011920311841
30	25.241440311018316	27.454771724485493	28.511298069506015	18.792489894990176
31	26.7577060342079	23.358514434993673	27.08374044780878	22.80003908298964
32	29.19396476359934	29.791265980314513	22.49020354009606	18.524565715990086
33	25.19670057287435	25.969875242983058	22.850435569634577	25.982988614508017
34	27.608789558670765	24.063550998158988	26.800903022760696	21.526756420409548
35	23.840880807166588	28.177549907949274	23.968414773370085	24.013154511514053
36	27.46608522148742	25.57698834709809	25.495993993561594	21.460932437852904
37	24.303705684517993	27.462999722305074	28.17523578356252	20.058058809614415
38	20.052659186045314	29.222505631036004	25.871396394079955	24.853438788838723
39	25.043196988552797	27.338037005420198	24.542317621285832	23.076448384741177
40	24.210883584115848	27.647872548313774	25.96036162050417	22.180882247066204
41	23.39708317477296	23.795112569295167	24.03552438058604	28.772279875345834
42	20.0696294315482	25.798115788499317	30.327114338314697	23.805140441637786
43	23.38114142899752	22.44494955208837	26.000987359738346	28.17292165917576
44	23.799226568204958	22.184739121044135	28.326168118565448	25.689866192185455
45	20.601363790638597	22.465005296773597	26.811702269898895	30.12192864268891
46	26.68159705437678	25.196443447942485	25.055538985282165	23.066420512398565
47	18.837229633134147	23.71360396589495	35.24514291003713	22.204023490933775
48	22.515916033282252	25.77291754517685	26.409301751535036	25.301864670005862
49	21.392794330909503	21.12204177765893	33.4761233788273	24.009040512604265
50	23.14998611525368	22.026864412880933	28.699256394697052	26.123893077168336
51	21.667403758138004	23.332287691943762	24.920291271122814	30.080017278795424
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184.0
1	305.0
2	426.0
3	1016.0
4	1606.0
5	1092.5
6	579.0
7	587.5
8	596.0
9	640.0
10	684.0
11	653.5
12	623.0
13	656.5
14	690.0
15	692.5
16	695.0
17	614.0
18	533.0
19	579.5
20	626.0
21	684.5
22	743.0
23	823.5
24	904.0
25	994.5
26	1718.0
27	2351.0
28	2806.5
29	3262.0
30	3719.0
31	4176.0
32	4863.5
33	5551.0
34	6572.0
35	7593.0
36	7900.0
37	8207.0
38	9035.5
39	9864.0
40	11904.0
41	13944.0
42	17000.5
43	20057.0
44	22444.5
45	24832.0
46	45666.5
47	66501.0
48	53366.5
49	40232.0
50	39924.0
51	39616.0
52	33878.5
53	28141.0
54	25116.5
55	22092.0
56	21427.5
57	20763.0
58	19293.0
59	17823.0
60	15310.0
61	12797.0
62	11337.0
63	9877.0
64	8509.0
65	7141.0
66	6204.0
67	5267.0
68	4413.5
69	3560.0
70	3089.5
71	2619.0
72	1990.0
73	1361.0
74	1024.0
75	521.5
76	356.0
77	250.0
78	144.0
79	109.0
80	74.0
81	56.5
82	39.0
83	22.5
84	6.0
85	3.5
86	1.0
87	2.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.155162589651052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91832822792497	23.34092696885847
2	10.361686297620686	6.45640042610664
3	3.7154544268806275	3.4726676029171415
4	1.8399779385044481	2.2929924734190816
5	1.136284225334689	1.7700559894178969
6	0.7246587086363433	1.3546115933743108
7	0.6087871635194188	1.327680414335798
8	0.4491363301150148	1.119433231172198
9	0.3680749723008658	1.0320692046493303
>10	5.649322826940095	41.07243281074776
>50	0.17700659708583466	3.410333349083016
>100	0.03970241429962647	2.3274996833094908
>500	0.003308534524968872	0.89110871400401
>1k	0.005789935418695526	3.056518350723413
>5k	0.001654267262484436	4.455285875192403
>10k+	8.27133631242218E-4	2.61998331268906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	10167	2.614189182239867	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	8947	2.300496765368357	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	8342	2.1449361815919117	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	2914	0.7492620514455564	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	1761	0.45279700500879366	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	1705	0.4383980088245277	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	1556	0.40008639397710555	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	1425	0.36640302790319756	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	1353	0.3478900328091413	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	1147	0.2949222968455913	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	996	0.2560964321344455	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.23089818881198	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	889	0.22858406442522292	TruSeq Adapter, Index 15 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	675	0.1735593290067778	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	461	0.1185345935883327	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATG	431	0.1108208456324759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24683993458741735	0.0
2	0.0	0.0	0.0	1.5602340865379671	0.0
3	0.0	0.0	0.0	1.9778049758816814	0.0
4	0.0	0.0	0.0	2.8067757562044244	0.0
5	0.0	0.0	0.0	6.229879974081807	0.0
6	0.0	0.0	0.0	7.343230929043804	0.0
7	0.0	0.0	0.0	8.51340649394728	0.0
8	0.0	0.0	0.0	9.764576412387251	0.0
9	0.0	0.0	0.0	10.134836314268377	0.0
10	0.0	0.0	0.0	13.382067078752224	0.0
11	0.0	0.0	0.0	14.666663238334241	0.0
12	0.0	0.0	0.0	18.49422497403038	0.0
13	0.0	0.0	0.0	19.120581308045953	0.0
14	0.0	0.0	0.0	19.454586594534554	0.0
15	0.0	0.0	0.0	20.212590893663414	0.0
16	0.0	0.0	0.0	20.925855454648303	0.0
17	0.0	0.0	0.0	21.69928724968888	0.0
18	0.0	0.0	0.0	22.527486655216038	0.0
19	0.0	0.0	0.0	23.566271379938083	0.0
20	0.0	0.0	0.0	24.183371216406627	0.0
21	0.0	0.0	0.0	24.78530068189532	0.0
22	0.0	0.0	0.0	25.520935111952195	0.0
23	0.0	0.0	0.0	26.12029332812227	0.0
24	0.0	0.0	0.0	26.619372820866204	0.0
25	0.0	0.0	0.0	27.069598576556377	0.0
26	0.0	0.0	0.0	27.555050447911633	0.0
27	0.0	0.0	0.0	28.223575270752555	0.0
28	2.5712493186189306E-4	0.0	0.0	28.663773154100113	0.0
29	2.5712493186189306E-4	0.0	0.0	29.18779376523465	0.0
30	2.5712493186189306E-4	0.0	0.0	29.78792335620031	0.0
31	2.5712493186189306E-4	0.0	0.0	30.285717224284937	0.0
32	5.142498637237861E-4	0.0	0.0	30.721801108722705	0.0
33	5.142498637237861E-4	0.0	0.0	31.168684240298678	0.0
34	5.142498637237861E-4	0.0	0.0	31.717388844891957	0.0
35	5.142498637237861E-4	0.0	0.0	32.23138158368388	0.0
36	5.142498637237861E-4	0.0	0.0	32.7366320747925	0.0
37	5.142498637237861E-4	0.0	0.0	33.19739995268901	0.0
38	7.713747955856792E-4	0.0	0.0	33.75330405537443	0.0
39	7.713747955856792E-4	0.0	0.0	34.70363780353598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAG	30	2.1617834E-6	45.000004	1
CGGGATA	35	1.209155E-7	45.000004	6
GCGTGAA	30	2.1617834E-6	45.000004	36
CGGTAGT	60	0.0	45.000004	12
TCGACGT	30	2.1617834E-6	45.000004	26
TCACGAC	25	3.8860293E-5	45.0	25
AACGTTA	20	7.027524E-4	45.0	45
GAATCCC	25	3.8860293E-5	45.0	8
CGTCTCG	20	7.027524E-4	45.0	42
TTGTACG	20	7.027524E-4	45.0	1
ACGGGTA	40	6.7957444E-9	45.0	5
CGTTCAT	25	3.8860293E-5	45.0	17
ACGGGCT	40	6.7957444E-9	45.0	5
CGCCTTA	20	7.027524E-4	45.0	11
CGGGGTA	20	7.027524E-4	45.0	6
ACTAGGA	20	7.027524E-4	45.0	32
AGTGCCG	20	7.027524E-4	45.0	38
ATGACCG	20	7.027524E-4	45.0	1
CCGACTA	20	7.027524E-4	45.0	42
CGGTCTA	20	7.027524E-4	45.0	31
>>END_MODULE