##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936477.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276496
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90516318500087	33.0	31.0	34.0	30.0	34.0
2	31.95289986111915	33.0	31.0	34.0	30.0	34.0
3	31.96866500781205	33.0	31.0	34.0	30.0	34.0
4	35.628681789248304	37.0	35.0	37.0	33.0	37.0
5	34.683250390602396	37.0	35.0	37.0	33.0	37.0
6	35.21672284590012	37.0	35.0	37.0	33.0	37.0
7	35.822348243735895	37.0	35.0	37.0	35.0	37.0
8	35.854811642844744	37.0	35.0	37.0	35.0	37.0
9	37.68625224234709	39.0	37.0	39.0	35.0	39.0
10	37.34102844164111	39.0	37.0	39.0	34.0	39.0
11	37.23653145072623	39.0	37.0	39.0	34.0	39.0
12	37.005696284937216	39.0	35.0	39.0	33.0	39.0
13	36.79440570568833	39.0	35.0	39.0	33.0	39.0
14	37.74139951391702	40.0	36.0	41.0	33.0	41.0
15	37.96504831896302	40.0	36.0	41.0	33.0	41.0
16	38.01729138938719	40.0	36.0	41.0	34.0	41.0
17	38.03508549852439	40.0	36.0	41.0	34.0	41.0
18	38.03507103176899	40.0	36.0	41.0	34.0	41.0
19	37.96565953937851	40.0	36.0	41.0	34.0	41.0
20	37.97132327411608	40.0	36.0	41.0	34.0	41.0
21	37.83771193796655	40.0	35.0	41.0	34.0	41.0
22	37.886341936230544	40.0	35.0	41.0	34.0	41.0
23	37.91901871998148	40.0	35.0	41.0	34.0	41.0
24	37.88989352468028	40.0	35.0	41.0	34.0	41.0
25	37.714386464903654	39.0	35.0	41.0	33.0	41.0
26	37.77263685550605	40.0	35.0	41.0	34.0	41.0
27	37.73748263989352	40.0	35.0	41.0	34.0	41.0
28	37.637705427926626	40.0	35.0	41.0	33.0	41.0
29	37.583820380765005	40.0	35.0	41.0	33.0	41.0
30	37.480654331346564	39.0	35.0	41.0	33.0	41.0
31	37.46056362479023	39.0	35.0	41.0	33.0	41.0
32	37.390294977142524	39.0	35.0	41.0	33.0	41.0
33	37.23319324691858	39.0	35.0	41.0	33.0	41.0
34	37.096363057693424	39.0	35.0	41.0	32.0	41.0
35	37.05640226260054	39.0	35.0	41.0	32.0	41.0
36	36.99731280018518	39.0	35.0	41.0	32.0	41.0
37	36.87658772640472	39.0	35.0	41.0	32.0	41.0
38	36.82249291128986	39.0	35.0	41.0	31.0	41.0
39	36.809548781899196	39.0	35.0	41.0	31.0	41.0
40	36.6665557548753	39.0	35.0	41.0	31.0	41.0
41	36.6196147503038	39.0	35.0	41.0	31.0	41.0
42	36.59442451247034	39.0	35.0	41.0	31.0	41.0
43	36.39217203865517	39.0	35.0	41.0	31.0	41.0
44	36.2429402233667	39.0	35.0	40.0	30.0	41.0
45	36.25282825067994	39.0	35.0	40.0	30.0	41.0
46	36.136515537295296	38.0	35.0	40.0	30.0	41.0
47	36.071444071523636	38.0	35.0	40.0	30.0	41.0
48	36.06602988831665	38.0	35.0	40.0	30.0	41.0
49	36.01143958682947	38.0	35.0	40.0	30.0	41.0
50	35.815425901278864	38.0	35.0	40.0	29.0	41.0
51	34.57170085643192	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	7.0
10	10.0
11	7.0
12	5.0
13	1.0
14	10.0
15	8.0
16	16.0
17	37.0
18	44.0
19	133.0
20	210.0
21	350.0
22	554.0
23	866.0
24	1173.0
25	1644.0
26	2195.0
27	2513.0
28	3007.0
29	3511.0
30	4210.0
31	5458.0
32	7377.0
33	10570.0
34	19800.0
35	28039.0
36	19377.0
37	29369.0
38	52610.0
39	83312.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.43563740524275	22.727272727272727	26.582663040333316	12.254426827151207
2	30.223945373531624	24.68787975232915	27.151929865169837	17.936245008969387
3	31.25614837104334	24.007580579827557	28.32626873444824	16.410002314680863
4	28.04778369307332	26.519370985475376	27.49153694809328	17.941308373358023
5	22.822029975117182	31.84169029570048	26.57181297378624	18.7644667553961
6	24.282810601238356	33.38710144088884	26.04703142179272	16.28305653608009
7	78.1736444650194	5.864822637578844	10.393640414327875	5.567892483073896
8	78.10926740350675	5.117976390255194	9.616775649557317	7.155980556680748
9	71.83395058156357	7.721630692668248	13.351730223945374	7.092688501822811
10	38.78645622359817	29.876019906255426	18.353249233261966	12.984274636884441
11	30.08542619061397	23.461098894739887	27.767128638388982	18.68634627625716
12	27.922646258897053	20.8089809617499	32.58925988079394	18.67911289855911
13	23.398530177651754	27.82354898443377	31.94223424570337	16.8356865922111
14	15.948874486430183	32.78890110526011	30.814188993692493	20.44803541461721
15	14.985750245934842	26.492607487992593	41.035675018806785	17.485967247265783
16	17.505135698165617	26.93203518314912	34.79363173427464	20.769197384410624
17	17.991218679474567	24.970704820322897	29.899528383774086	27.138548116428446
18	20.326876338174873	25.001085006654705	32.85761819339159	21.814420461778834
19	20.93375672704126	26.66476187720618	29.96969214744517	22.43178924830739
20	22.479529541114516	26.787367629188125	29.88867831722701	20.844424512470344
21	21.399947919680574	28.114330767895378	30.894117817255946	19.5916034951681
22	18.897199236155316	26.56783461605231	29.68108037729298	24.853885770499392
23	17.2414790810717	27.619567733348767	28.966784329610558	26.172168855968987
24	19.49829292286326	24.876309241363348	32.29305306405879	23.3323447717146
25	17.406400092587234	27.602569295758343	30.06372605751982	24.9273045541346
26	18.555783808807362	28.405835889126784	29.895188357155256	23.143191944910598
27	19.175322608645335	31.10641745269371	29.057563219721082	20.660696718939874
28	15.46532318731555	27.209435217869338	32.60951333834847	24.71572825646664
29	18.111654418147097	26.9284184943001	31.919087437069614	23.04083965048319
30	20.147126902378336	27.743620160870318	30.39863144493953	21.710621491811814
31	22.425279208379145	29.098793472599965	25.09548058561426	23.380446733406632
32	22.993822695445864	31.812033447138475	24.85786412823332	20.336279729182337
33	23.840127886117703	27.747236849719343	27.205095191250507	21.207540072912447
34	21.5048318963023	26.79243099357676	28.289740177073085	23.412996933047854
35	19.490697876280308	28.89155720155084	27.520470458885484	24.097274463283373
36	24.898009374457498	26.721543892135873	28.61668884902494	19.76375788438169
37	21.1565447601412	28.530973323303048	29.731352352294426	20.581129564261328
38	18.689962965106186	31.20551472715699	26.7106938255888	23.393828482148024
39	22.846985128175454	25.520441525374686	30.729920143510213	20.902653202939643
40	24.94647300503443	24.25894045483479	30.120146403564608	20.674440136566172
41	21.845162316995545	25.667279092645103	26.648848446270467	25.838710144088882
42	24.520788727504193	25.284995081303162	27.416671488918464	22.777544702274174
43	24.47485677912158	25.98844106243852	26.939630229732074	22.59707192870783
44	20.450205427926623	25.665470748220585	28.058633759620395	25.825690064232393
45	20.399210115155373	26.282477865864244	26.903463341241828	26.414848677738554
46	22.558735026908163	26.76385915166946	28.83115849777212	21.846247323650253
47	20.204993923962732	25.862941959377352	30.52955558127423	23.40250853538568
48	20.592341299693302	24.396374631097736	29.741117412186796	25.270166657022163
49	22.429619234997975	21.738108327064406	33.37733638099647	22.45493605694115
50	21.945344598113536	23.00467276199294	30.675308141889936	24.374674498003586
51	20.0552630056131	23.534879347259995	27.13312308315491	29.27673456397199
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	258.0
1	334.0
2	410.0
3	833.0
4	1256.0
5	863.0
6	470.0
7	507.5
8	545.0
9	543.5
10	542.0
11	563.5
12	585.0
13	588.5
14	592.0
15	568.0
16	544.0
17	606.5
18	669.0
19	720.0
20	771.0
21	777.0
22	783.0
23	1055.5
24	1328.0
25	1513.5
26	2264.5
27	2830.0
28	2963.0
29	3096.0
30	3639.0
31	4182.0
32	5121.5
33	6061.0
34	6216.0
35	6371.0
36	8001.5
37	9632.0
38	10191.0
39	10750.0
40	11919.0
41	13088.0
42	16711.0
43	20334.0
44	22428.5
45	24523.0
46	28263.0
47	32003.0
48	34769.5
49	37536.0
50	35041.0
51	32546.0
52	26446.0
53	20346.0
54	16490.5
55	12635.0
56	10937.0
57	9239.0
58	7968.0
59	6697.0
60	5841.5
61	4986.0
62	4236.0
63	3486.0
64	2900.5
65	2315.0
66	1861.0
67	1407.0
68	1101.0
69	795.0
70	690.0
71	585.0
72	475.0
73	365.0
74	244.0
75	83.0
76	43.0
77	38.0
78	33.0
79	18.5
80	4.0
81	4.0
82	4.0
83	15.5
84	27.0
85	13.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	276496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.37555697008275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99717826033616	25.823158382038073
2	11.84006052427105	8.376974712111569
3	4.4830900094057995	4.757754180892309
4	2.18275876170613	3.0886522770672995
5	1.2442236126446653	2.2007551646316763
6	0.8311863575021471	1.7642208205543661
7	0.5878624299676931	1.4557172617325385
8	0.44370833844518054	1.2557143683814593
9	0.3476056107635055	1.106706787801632
>10	4.716190242506032	38.735460910826916
>50	0.2699055330634278	5.909307910421851
>100	0.050096102727681675	2.9848533071002836
>500	0.003067108330266225	0.7284011341936231
>1k	0.003067108330266225	1.8123227822463979
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1954	0.7067010010994734	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1947	0.704169318905156	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	1110	0.40145246224176845	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.26546496151843063	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	718	0.2596782593599907	No Hit
ACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	562	0.20325791331520168	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC	399	0.14430588507609513	No Hit
ATGAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	385	0.1392425206874602	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	318	0.11501070539899311	No Hit
GCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	308	0.11139401654996818	No Hit
AGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	305	0.11030900989526068	No Hit
ACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	280	0.10126728777269833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.424599270875528	0.0
2	0.0	0.0	0.0	2.42607487992593	0.0
3	0.0	0.0	0.0	3.20908801573983	0.0
4	0.0	0.0	0.0	4.183062322782247	0.0
5	0.0	0.0	0.0	6.520889994791968	0.0
6	0.0	0.0	0.0	7.774434349864013	0.0
7	0.0	0.0	0.0	9.20664313407789	0.0
8	0.0	0.0	0.0	11.322406110757479	0.0
9	0.0	0.0	0.0	12.100355882182743	0.0
10	0.0	0.0	0.0	13.722802499855332	0.0
11	0.0	0.0	0.0	16.0523117875123	0.0
12	0.0	0.0	0.0	18.14384294890342	0.0
13	0.0	0.0	0.0	19.00063653723743	0.0
14	0.0	0.0	0.0	19.44476592789769	0.0
15	0.0	0.0	0.0	19.950017360106475	0.0
16	3.6166888490249405E-4	0.0	0.0	21.043704068051618	0.0
17	3.6166888490249405E-4	0.0	0.0	22.406834095249117	0.0
18	3.6166888490249405E-4	0.0	0.0	24.194925062207048	0.0
19	3.6166888490249405E-4	0.0	0.0	25.06799375036167	0.0
20	7.233377698049881E-4	0.0	0.0	25.933467391933338	0.0
21	7.233377698049881E-4	0.0	0.0	27.017389039986114	0.0
22	7.233377698049881E-4	0.0	0.0	28.002213413575603	0.0
23	7.233377698049881E-4	0.0	0.0	28.964252647416238	0.0
24	0.0010850066547074821	0.0	0.0	29.70712053700596	0.0
25	0.0010850066547074821	0.0	0.0	30.379824662924598	0.0
26	0.0010850066547074821	0.0	0.0	31.01925525143221	0.0
27	0.0010850066547074821	0.0	0.0	31.617455587060935	0.0
28	0.0010850066547074821	0.0	0.0	32.233739366934785	0.0
29	0.0010850066547074821	0.0	0.0	32.91150685724206	0.0
30	0.0010850066547074821	0.0	0.0	33.65256640240727	0.0
31	0.0010850066547074821	0.0	0.0	34.31984549505237	0.0
32	0.0010850066547074821	0.0	0.0	34.934320930501706	0.0
33	0.0010850066547074821	0.0	0.0	35.52347954400787	0.0
34	0.0010850066547074821	0.0	0.0	36.201608703200044	0.0
35	0.0010850066547074821	0.0	0.0	36.880099531277125	0.0
36	0.0010850066547074821	0.0	0.0	37.448281349458945	0.0
37	0.0014466755396099762	0.0	0.0	38.04105665181413	0.0
38	0.0018083444245124702	0.0	0.0	38.63347028528442	0.0
39	0.0018083444245124702	0.0	0.0	39.309429431167175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAG	20	7.023982E-4	45.000004	1
TTAGGCG	20	7.023982E-4	45.000004	1
CCGGGTT	20	7.023982E-4	45.000004	6
GTACCCC	20	7.023982E-4	45.000004	33
ACCGGGC	25	3.883095E-5	45.000004	4
TCCGCAG	30	2.1595006E-6	45.000004	1
CAAGTCG	25	3.883095E-5	45.000004	1
GTCTGCG	20	7.023982E-4	45.000004	1
CGTGAGG	30	2.1595006E-6	45.000004	2
CGGCAGG	30	2.1595006E-6	45.000004	2
GGCCGGA	20	7.023982E-4	45.000004	27
CAACCTA	20	7.023982E-4	45.000004	37
TAAACGG	40	6.7830115E-9	45.000004	2
TCGGGCG	20	7.023982E-4	45.000004	5
GTCTATC	20	7.023982E-4	45.000004	43
GTTAGCT	20	7.023982E-4	45.000004	17
AGGAATA	20	7.023982E-4	45.000004	29
TAACGGG	30	2.1595006E-6	45.000004	3
TACGGAT	20	7.023982E-4	45.000004	4
GGCCCGA	20	7.023982E-4	45.000004	11
>>END_MODULE