##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538876
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9344246171661	33.0	31.0	34.0	30.0	34.0
2	31.972708007036868	33.0	31.0	34.0	30.0	34.0
3	32.000393411471286	33.0	31.0	34.0	30.0	34.0
4	35.69240047803205	37.0	35.0	37.0	33.0	37.0
5	34.76423147440227	37.0	35.0	37.0	33.0	37.0
6	35.26867034345564	37.0	35.0	37.0	33.0	37.0
7	35.85526911571493	37.0	35.0	37.0	35.0	37.0
8	35.86121668064638	37.0	35.0	37.0	35.0	37.0
9	37.70038932889941	39.0	37.0	39.0	35.0	39.0
10	37.36843541000156	39.0	37.0	39.0	34.0	39.0
11	37.26298072283791	39.0	37.0	39.0	34.0	39.0
12	37.10553819431558	39.0	37.0	39.0	34.0	39.0
13	36.98354166821309	39.0	37.0	39.0	33.0	39.0
14	37.95225246624455	40.0	37.0	41.0	33.0	41.0
15	38.10927003614932	40.0	37.0	41.0	33.0	41.0
16	38.15142073501139	40.0	37.0	41.0	34.0	41.0
17	38.15038895775652	40.0	37.0	41.0	34.0	41.0
18	38.15603775265553	40.0	37.0	41.0	34.0	41.0
19	38.10319999406171	40.0	37.0	41.0	34.0	41.0
20	38.10516890713262	40.0	36.0	41.0	34.0	41.0
21	38.03000690325789	40.0	36.0	41.0	34.0	41.0
22	38.07295927077843	40.0	36.0	41.0	34.0	41.0
23	38.075759543939604	40.0	36.0	41.0	34.0	41.0
24	38.04424208908914	40.0	36.0	41.0	34.0	41.0
25	37.91179789042377	40.0	36.0	41.0	34.0	41.0
26	37.95756166539241	40.0	36.0	41.0	34.0	41.0
27	37.95764146111536	40.0	36.0	41.0	34.0	41.0
28	37.891919476836975	40.0	36.0	41.0	34.0	41.0
29	37.88867197648439	40.0	36.0	41.0	34.0	41.0
30	37.780209176137	40.0	36.0	41.0	34.0	41.0
31	37.747082816826136	40.0	36.0	41.0	33.0	41.0
32	37.71242363734885	40.0	36.0	41.0	33.0	41.0
33	37.57944684862566	40.0	36.0	41.0	33.0	41.0
34	37.51251122707265	40.0	36.0	41.0	33.0	41.0
35	37.456579992428686	40.0	35.0	41.0	33.0	41.0
36	37.36940223724939	40.0	35.0	41.0	33.0	41.0
37	37.324599722385116	40.0	35.0	41.0	33.0	41.0
38	37.25671026358569	40.0	35.0	41.0	33.0	41.0
39	37.217651185059275	39.0	35.0	41.0	33.0	41.0
40	37.110535633429585	39.0	35.0	41.0	32.0	41.0
41	37.04160697451733	39.0	35.0	41.0	32.0	41.0
42	37.058950482114625	39.0	35.0	41.0	32.0	41.0
43	36.9482608243826	39.0	35.0	41.0	32.0	41.0
44	36.83839139245392	39.0	35.0	41.0	31.0	41.0
45	36.844708244568324	39.0	35.0	41.0	32.0	41.0
46	36.73292371528886	39.0	35.0	41.0	31.0	41.0
47	36.63794082497643	39.0	35.0	41.0	31.0	41.0
48	36.60255791684914	39.0	35.0	41.0	31.0	41.0
49	36.60632687297263	39.0	35.0	41.0	31.0	41.0
50	36.47998983068461	39.0	35.0	40.0	31.0	41.0
51	35.28062485618213	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	8.0
11	11.0
12	7.0
13	8.0
14	11.0
15	23.0
16	25.0
17	39.0
18	97.0
19	188.0
20	323.0
21	548.0
22	827.0
23	1179.0
24	1786.0
25	2434.0
26	3337.0
27	4049.0
28	4797.0
29	6017.0
30	7490.0
31	9761.0
32	12968.0
33	19220.0
34	35685.0
35	48168.0
36	38783.0
37	57318.0
38	103786.0
39	179871.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22242593843481	22.073723825147155	25.73393507968438	11.969915156733645
2	30.38899487080516	25.49900162560589	26.493293447843293	17.618710055745662
3	31.389596122299007	23.861704733556515	28.568353387421226	16.180345756723252
4	29.200780884656208	25.557085489054998	26.9837587868081	18.258374839480695
5	24.693992681062063	30.469718450998002	26.32665028689346	18.509638581046474
6	25.65395378528641	33.22118632115737	25.53036320043943	15.594496693116783
7	77.06336893830863	6.3432032601192105	11.242103934856999	5.351323866715163
8	76.81822905455059	5.59368017874242	10.376969840928155	7.211120925778844
9	70.31005277652001	7.881590570001262	13.488260750154025	8.320095903324699
10	40.14504264431892	25.483228052464764	19.631417988553952	14.740311314662371
11	29.703679510685205	24.472605942740074	26.5320778806256	19.291636665949124
12	26.20677113102087	21.67641535343938	32.09383977018832	20.022973745351436
13	23.1400173694876	25.87738180954431	32.89383828561673	18.08876253535136
14	18.965958773446953	29.44258048233731	30.79391177191042	20.797548972305314
15	16.722956672778153	25.472093765541608	39.133121534453196	18.671828027227043
16	19.329864384385274	26.031962826327394	32.39131080248517	22.24686198680216
17	19.57741669697667	25.735048508376696	29.466704770670805	25.220830023975832
18	20.19945219308338	25.477660909003184	32.89810642893727	21.424780468976167
19	21.82078994054291	26.799115195332508	29.538892064222566	21.841202799902018
20	21.87757480385098	27.91978117414767	30.38880929935644	19.813834722644913
21	21.741550931939816	27.406490546990405	31.088784803925208	19.763173717144575
22	19.210913085756278	25.525723914221455	29.33643361366994	25.92692938635233
23	18.90991619593376	26.43372501280443	30.000408257187182	24.65595053407463
24	21.376531892309178	24.165299623661102	30.87834678107765	23.57982170295207
25	18.498689865572043	27.782829444992913	29.316763040105702	24.401717649329342
26	18.329263132891427	27.302941678605098	30.90154321216755	23.46625197633593
27	21.21378573178245	26.871488060332986	29.54575820782518	22.368968000059382
28	16.762854534252778	25.309718747912317	32.205182639419824	25.722244078415073
29	18.170970687133963	23.640132423785808	32.392609802626204	25.79628708645403
30	22.146282261596358	24.376294360854818	30.683682331371227	22.793741046177598
31	20.989986564627113	25.347018609104875	28.588580675331617	25.07441415093639
32	22.79077190299809	24.707910539716003	28.19108663217512	24.310230925110787
33	22.07724968267282	22.729904467818198	31.191776957964358	24.001068891544623
34	18.867605905625783	22.712275180189874	32.88938457084747	25.53073434333687
35	20.982006992332188	21.878131518197137	31.6738915817368	25.465969907733875
36	22.646211744445846	21.762334934196364	33.8743978206489	21.71705550070888
37	20.581358234547466	23.213318091731676	33.17145317290063	23.033870500820225
38	20.129677328364966	24.825933981101404	29.046199867873128	25.998188822660502
39	19.787854719824228	21.96664909923619	34.367832302793225	23.877663878146365
40	22.537095732598967	20.974769705832138	34.37024473162657	22.117889829942325
41	20.784930113792413	22.239067985955952	31.176560099169382	25.799441801082253
42	22.533013160727144	21.032296854935087	32.02183804808527	24.412851936252498
43	23.227607093283055	20.578389091367956	31.00212293737335	25.191880877975635
44	19.383865675962557	22.053310965788047	30.949791788834535	27.613031569414858
45	20.40692107275143	21.331066887372977	30.324044863753443	27.937967176122154
46	23.700072001722102	21.333479316206326	31.660530437429017	23.30591824464255
47	19.315946525731338	20.79439425767709	35.849434749367205	24.040224467224373
48	20.209844194211655	20.60139995100914	33.020583585091934	26.168172269687272
49	21.75194293306809	18.436894573148553	35.50260171171104	24.308560782072313
50	20.517336084739345	19.179737082371453	33.58305064615978	26.71987618672941
51	19.02144463661399	19.901610017889087	30.263734142919706	30.813211202577218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	462.5
2	560.0
3	1276.5
4	1993.0
5	1352.0
6	711.0
7	769.0
8	827.0
9	887.5
10	948.0
11	975.0
12	1002.0
13	1091.5
14	1181.0
15	1146.0
16	1111.0
17	1156.5
18	1202.0
19	1273.5
20	1345.0
21	1469.5
22	1594.0
23	1897.0
24	2200.0
25	2525.0
26	3717.5
27	4585.0
28	5109.5
29	5634.0
30	6877.0
31	8120.0
32	9156.0
33	10192.0
34	11771.0
35	13350.0
36	14437.5
37	15525.0
38	17079.0
39	18633.0
40	21060.5
41	23488.0
42	28143.5
43	32799.0
44	37894.0
45	42989.0
46	52890.5
47	62792.0
48	67461.0
49	72130.0
50	69497.5
51	66865.0
52	54104.5
53	41344.0
54	34633.5
55	27923.0
56	24576.0
57	21229.0
58	17965.5
59	14702.0
60	13408.0
61	12114.0
62	10567.5
63	9021.0
64	7667.5
65	6314.0
66	5185.0
67	4056.0
68	3463.0
69	2870.0
70	2317.5
71	1765.0
72	1406.0
73	1047.0
74	835.5
75	460.0
76	296.0
77	350.5
78	405.0
79	225.0
80	45.0
81	60.5
82	76.0
83	56.0
84	36.0
85	22.5
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.145234938619616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5576143175415	22.359458540072634
2	13.039622933574504	8.383234854215296
3	5.114012590889261	4.9317340863958234
4	2.565190435616512	3.2983459686077112
5	1.5541130008097863	2.497866376609686
6	0.987836700623173	1.9052545693513647
7	0.7208535743827493	1.622040525341403
8	0.5215740857758315	1.3412897220127877
9	0.4058330363887484	1.1741038470512712
>10	5.148682584150867	38.842612132861575
>50	0.3156239919976113	6.174768209052096
>100	0.06092007198483292	3.630483205189315
>500	0.0029009558088015676	0.559511436122878
>1k	0.005221720455842822	3.2792965271161885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3748	0.6955217897995086	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3238	0.6008803509527239	No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	2467	0.4578047639902315	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	1561	0.28967703145064916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	1540	0.28578003102754623	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	1413	0.2622124570402096	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	1406	0.26091345689917533	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1135	0.21062359429627597	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	1075	0.19948930737312479	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	676	0.12544629933416965	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCC	618	0.11468315530845687	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	583	0.10818815460328535	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	573	0.1063324401160935	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	550	0.10206429679555222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48452705260579426	0.0
2	0.0	0.0	0.0	2.3756856865030174	0.0
3	0.0	0.0	0.0	3.1903443463802432	0.0
4	0.0	0.0	0.0	4.195213741194634	0.0
5	0.0	0.0	0.0	6.856679458725198	0.0
6	0.0	0.0	0.0	8.138978169374772	0.0
7	0.0	0.0	0.0	9.58921904111521	0.0
8	0.0	0.0	0.0	11.706403699552402	0.0
9	0.0	0.0	0.0	12.48636049851914	0.0
10	0.0	0.0	0.0	14.39384941990365	0.0
11	0.0	0.0	0.0	16.80553596745819	0.0
12	0.0	0.0	0.0	18.890802336715684	0.0
13	0.0	0.0	0.0	19.63420156028474	0.0
14	0.0	0.0	0.0	19.921837305799478	0.0
15	0.0	0.0	0.0	20.505459512021318	0.0
16	0.0	0.0	0.0	21.702580927708787	0.0
17	0.0	0.0	0.0	23.13352236878243	0.0
18	0.0	0.0	0.0	24.75615911638299	0.0
19	0.0	0.0	0.0	25.72094507827404	0.0
20	0.0	0.0	0.0	26.60426517417736	0.0
21	0.0	0.0	0.0	27.65942443159465	0.0
22	0.0	0.0	0.0	28.777863553025185	0.0
23	0.0	0.0	0.0	29.860116241955478	0.0
24	0.0	0.0	0.0	30.69574447553797	0.0
25	0.0	0.0	0.0	31.464752559030277	0.0
26	0.0	0.0	0.0	32.1255724879193	0.0
27	0.0	0.0	0.0	32.862476710783184	0.0
28	0.0	0.0	0.0	33.51346135289009	0.0
29	0.0	0.0	0.0	34.19283842665103	0.0
30	0.0	0.0	0.0	35.027909945887366	0.0
31	0.0	0.0	0.0	35.76370074005894	0.0
32	0.0	0.0	0.0	36.444376813960915	0.0
33	0.0	0.0	0.0	37.08478388349082	0.0
34	0.0	0.0	0.0	37.70385023641803	0.0
35	0.0	0.0	0.0	38.43203260119211	0.0
36	0.0	0.0	0.0	39.055923811786016	0.0
37	0.0	0.0	0.0	39.67684587920041	0.0
38	0.0	0.0	0.0	40.30073708979431	0.0
39	0.0	0.0	0.0	41.01407373867087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	30	2.1633477E-6	45.000004	21
AGATCGT	25	3.888037E-5	45.000004	23
TAGACGG	60	0.0	45.000004	2
TTGTCGC	30	2.1633477E-6	45.000004	38
AGGCGAT	25	3.888037E-5	45.000004	6
CGTTCCA	25	3.888037E-5	45.000004	14
CGTTAGG	55	1.8189894E-12	45.000004	2
CTACGAA	30	2.1633477E-6	45.000004	11
TGCGACG	25	3.888037E-5	45.000004	1
ATAAGCG	25	3.888037E-5	45.000004	1
CGAATAT	30	2.1633477E-6	45.000004	14
GATTCGA	25	3.888037E-5	45.000004	22
CCACGAC	30	2.1633477E-6	45.000004	11
CGATAGT	25	3.888037E-5	45.000004	10
GCGTAAG	60	0.0	45.000004	1
AATCGCG	30	2.1633477E-6	45.000004	1
GACTACG	25	3.888037E-5	45.000004	1
TGTCCGA	25	3.888037E-5	45.000004	4
CGTATGG	45	3.8380676E-10	45.0	2
CGAGTAT	20	7.029949E-4	45.0	43
>>END_MODULE