FastQCFastQC Report
Sat 14 Jan 2017
SRR2936475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474839
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC40050.8434437777857338No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC36170.76173187122372Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG36060.7594152965531475No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC35470.7469900324109856No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC23830.5018543127249446Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT17180.3618068440039676Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC12140.2556656045522798Illumina PCR Primer Index 11 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC8640.18195641048860772Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT7950.16742516937319807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.1429958364835239No Hit
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT5870.12362084832964436Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT5850.12319965293499481No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA5630.1185665035938497No Hit
AGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG5010.1055094463597135Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC4940.10403526247844007No Hit
ACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG4780.10066569932124363Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA302.1628002E-645.00000410
ACACGCG351.209919E-745.00000436
TCTAGCG302.1628002E-645.0000041
CGTTAGT302.1628002E-645.00000412
TATTACG351.209919E-745.0000041
GCGGCTA302.1628002E-645.00000424
CGATACG302.1628002E-645.00000410
TAATACG253.8873353E-545.04
GTCGATG207.0291007E-445.01
GATCGTT207.0291007E-445.010
GAATCGG253.8873353E-545.02
TTCGTAA207.0291007E-445.013
CGCGACT253.8873353E-545.027
ACGGGCC453.8380676E-1045.05
ACTAGCG253.8873353E-545.01
GTCAACG253.8873353E-545.01
GTCCGGC207.0291007E-445.044
CATACGA406.8012014E-945.018
ACATCGC207.0291007E-445.015
CCAGTCG253.8873353E-545.028
Produced by FastQC (version 0.11.5)