##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474839
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90386215117124	33.0	31.0	34.0	30.0	34.0
2	31.949210153336182	33.0	31.0	34.0	30.0	34.0
3	32.008381788353525	33.0	31.0	34.0	30.0	34.0
4	35.66804959154577	37.0	35.0	37.0	33.0	37.0
5	34.710619810082996	37.0	35.0	37.0	33.0	37.0
6	35.22607452210118	37.0	35.0	37.0	33.0	37.0
7	35.82571566362493	37.0	35.0	37.0	35.0	37.0
8	35.80385351666565	37.0	35.0	37.0	35.0	37.0
9	37.67692839046498	39.0	37.0	39.0	35.0	39.0
10	37.28992984990702	39.0	37.0	39.0	34.0	39.0
11	37.22759924942981	39.0	37.0	39.0	34.0	39.0
12	37.107895518270404	39.0	37.0	39.0	34.0	39.0
13	36.91078028552836	39.0	37.0	39.0	33.0	39.0
14	37.94681776349457	40.0	37.0	41.0	33.0	41.0
15	38.10254001882743	40.0	37.0	41.0	33.0	41.0
16	38.17984622156141	40.0	37.0	41.0	34.0	41.0
17	38.16029011938784	40.0	37.0	41.0	34.0	41.0
18	38.159919467440545	40.0	37.0	41.0	34.0	41.0
19	38.103719365932456	40.0	37.0	41.0	34.0	41.0
20	38.056490726330395	40.0	36.0	41.0	34.0	41.0
21	38.00864503547518	40.0	36.0	41.0	34.0	41.0
22	38.06641619580532	40.0	36.0	41.0	34.0	41.0
23	38.05877992330032	40.0	36.0	41.0	34.0	41.0
24	38.017157394401046	40.0	36.0	41.0	34.0	41.0
25	37.89540033569273	40.0	36.0	41.0	34.0	41.0
26	37.9241279675848	40.0	36.0	41.0	34.0	41.0
27	37.92863686428453	40.0	36.0	41.0	34.0	41.0
28	37.83355621589634	40.0	36.0	41.0	33.0	41.0
29	37.81767083158713	40.0	36.0	41.0	34.0	41.0
30	37.73810702153783	40.0	36.0	41.0	33.0	41.0
31	37.666870244440744	40.0	36.0	41.0	33.0	41.0
32	37.61544649870798	40.0	36.0	41.0	33.0	41.0
33	37.49922184150839	40.0	36.0	41.0	33.0	41.0
34	37.430554356318666	40.0	36.0	41.0	33.0	41.0
35	37.41227447619088	40.0	35.0	41.0	33.0	41.0
36	37.350146049503095	40.0	35.0	41.0	33.0	41.0
37	37.27657584991966	40.0	35.0	41.0	33.0	41.0
38	37.22679476622603	40.0	35.0	41.0	33.0	41.0
39	37.1961422713804	39.0	35.0	41.0	33.0	41.0
40	37.069078150699504	39.0	35.0	41.0	32.0	41.0
41	36.99236162151803	39.0	35.0	41.0	32.0	41.0
42	37.08313344101896	39.0	35.0	41.0	32.0	41.0
43	36.961881816784214	39.0	35.0	41.0	32.0	41.0
44	36.83235791499856	39.0	35.0	41.0	31.0	41.0
45	36.799412011229066	39.0	35.0	41.0	31.0	41.0
46	36.69685514458585	39.0	35.0	41.0	31.0	41.0
47	36.557264672868065	39.0	35.0	41.0	31.0	41.0
48	36.559682334433354	39.0	35.0	41.0	31.0	41.0
49	36.58983360675934	39.0	35.0	41.0	31.0	41.0
50	36.47424284862869	39.0	35.0	40.0	31.0	41.0
51	35.32960013815209	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	4.0
12	6.0
13	5.0
14	9.0
15	17.0
16	29.0
17	44.0
18	85.0
19	171.0
20	359.0
21	530.0
22	828.0
23	1251.0
24	1659.0
25	2271.0
26	3037.0
27	3629.0
28	4370.0
29	5216.0
30	6666.0
31	8739.0
32	11645.0
33	16888.0
34	30352.0
35	42773.0
36	33939.0
37	51632.0
38	93326.0
39	155253.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7656068688545	22.55438159039169	25.086187107630163	11.593824433123649
2	28.54083173454581	27.242918125933212	26.19519458174244	18.02105555777853
3	29.56117757808436	26.420744715577282	27.656742601176397	16.361335105161963
4	27.107714404250704	25.254454667792665	29.87075619315178	17.76707473480485
5	25.1731639566253	29.781041574091425	25.976804769616653	19.068989699666623
6	23.90557641642746	34.40597760504087	25.82917578379198	15.859270194739691
7	76.95071382089507	5.77732663071062	11.694279534747567	5.577680013646731
8	76.48908366835917	6.769031187412996	9.77910407527604	6.962781068951792
9	71.49412748321011	6.191993496743107	12.237832191542818	10.076046828503978
10	39.808440334513385	23.678130903316703	19.863364213975686	16.65006454819423
11	29.046476805822607	26.563529954363478	25.46589475590674	18.924098483907176
12	27.556919292644455	21.746107628059193	30.749580384088084	19.947392695208272
13	22.096121001012975	26.75706923820495	33.62276476869002	17.524044992092055
14	17.223522078009598	30.80602056697112	30.266679864122363	21.703777490896915
15	14.991607681761607	25.9033061732503	40.256802832117835	18.848283312870258
16	17.448229821055136	24.884855708987676	34.07323324326772	23.593681226689466
17	17.755913056846637	25.14894522143295	29.968894720105133	27.126247001615283
18	19.85788867384524	25.78284429038053	32.35475603309753	22.004511002676697
19	21.050082238400805	27.00009898091774	28.73668759305786	23.213131187623596
20	22.106650885879215	27.81026832252616	30.512026181505732	19.571054610088893
21	20.8196883575275	29.080383035091895	29.953521088200425	20.146407519180183
22	19.05066769999937	25.700079395331894	28.299697371108945	26.949555533559792
23	17.21257099774871	28.414473116150944	29.503684406714697	24.86927147938564
24	22.1192867477187	23.706772190152872	30.490124020983956	23.683817041144472
25	17.846891262090942	29.459669487973816	28.099629558650406	24.593809691284836
26	17.832570618672854	27.904826688624983	28.79607614370345	25.466526548998715
27	20.200952322787302	28.24367838362055	28.808922603240255	22.74644669035189
28	15.900126148020696	28.335077784259504	31.807833813145088	23.95696225457471
29	17.293019318126778	26.76064939905947	29.851381204997907	26.09495007781585
30	18.432984653745795	27.340635457491906	30.228772278603905	23.997607610158393
31	19.576740747916663	30.95322835740114	24.778925067233317	24.691105827448883
32	20.001726901118065	30.909002840962934	24.244638709120355	24.844631548798645
33	18.734771154012204	27.51669513245542	25.205174806618665	28.543358906913713
34	17.457917315132075	28.845566602574767	26.541206598447054	27.155309483846104
35	17.147496309275354	25.820541278201663	26.731797514525972	30.300164897997007
36	19.15301818089921	26.663353262895424	26.715792089529295	27.467836466676072
37	16.90973150899568	30.102624257906363	30.14221662500342	22.845427608094532
38	16.44557418409187	30.354499103906797	25.012056718171845	28.18786999382949
39	19.101211147357315	28.245573762896477	26.997993003944494	25.655222085801714
40	20.238859908305763	24.5561127034637	28.1973468902091	27.007680498021436
41	16.49717061993644	26.772442870109657	25.99091481533741	30.739471694616494
42	18.70297090171616	24.260854731814362	28.49618502271296	28.539989343756517
43	21.081461295302198	24.371629120607196	27.761620254444136	26.78528932964647
44	19.348663441713928	26.706315193149678	27.064963071693775	26.880058293442623
45	18.18679594557313	26.135174238004883	26.238788305088672	29.439241511333314
46	21.995244703994405	25.916784425879086	27.22375373547666	24.86421713464985
47	16.921314382348545	25.38776301019925	31.854375904253864	25.836546703198348
48	17.84583827360432	24.21178546833769	29.25054597453031	28.69183028352768
49	18.607780742525364	21.365557588993322	32.95769724053837	27.068964427942944
50	19.27432245455828	22.73949696634017	29.993955846086777	27.99222473301477
51	17.213623986235334	23.145108131387694	26.778971398726725	32.86229648365025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	365.5
2	460.0
3	936.0
4	1412.0
5	1011.0
6	610.0
7	594.5
8	579.0
9	646.5
10	714.0
11	730.5
12	747.0
13	790.0
14	833.0
15	780.5
16	728.0
17	801.5
18	875.0
19	966.0
20	1057.0
21	1144.5
22	1232.0
23	1305.5
24	1379.0
25	2004.5
26	3226.0
27	3822.0
28	4627.5
29	5433.0
30	6383.5
31	7334.0
32	8470.0
33	9606.0
34	11267.5
35	12929.0
36	13614.0
37	14299.0
38	16491.0
39	18683.0
40	20972.5
41	23262.0
42	27348.0
43	31434.0
44	35257.0
45	39080.0
46	51795.5
47	64511.0
48	62477.5
49	60444.0
50	56976.5
51	53509.0
52	42771.5
53	32034.0
54	27060.0
55	22086.0
56	19034.0
57	15982.0
58	14478.5
59	12975.0
60	12555.0
61	12135.0
62	10275.0
63	8415.0
64	7073.5
65	5732.0
66	4240.5
67	2749.0
68	2147.0
69	1545.0
70	1432.5
71	1320.0
72	1159.5
73	999.0
74	777.5
75	429.5
76	303.0
77	196.5
78	90.0
79	60.0
80	30.0
81	19.0
82	8.0
83	5.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.769907093849657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.61614014843374	21.72852071693573
2	12.332278042040542	7.589260992182193
3	4.964122491893532	4.582367636341603
4	2.4938293363828494	3.069395879536681
5	1.5399690055763375	2.3692351614495974
6	0.9888334822013952	1.8255788627174863
7	0.6372277448031777	1.372520695365198
8	0.4842295717187836	1.1919759147105264
9	0.3852813866432772	1.0669565224802886
>10	5.01530790896979	37.975260530996394
>50	0.47287728298262943	8.802564238384731
>100	0.06030898681517593	3.2253364642238096
>500	0.004797305769388994	0.9645432486014843
>1k	0.004797305769388994	4.236483136074294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	4005	0.8434437777857338	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3617	0.76173187122372	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	3606	0.7594152965531475	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	3547	0.7469900324109856	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	2383	0.5018543127249446	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1718	0.3618068440039676	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1214	0.2556656045522798	Illumina PCR Primer Index 11 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	864	0.18195641048860772	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	795	0.16742516937319807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.1429958364835239	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	587	0.12362084832964436	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	585	0.12319965293499481	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	563	0.1185665035938497	No Hit
AGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	501	0.1055094463597135	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	494	0.10403526247844007	No Hit
ACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	478	0.10066569932124363	Illumina PCR Primer Index 11 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.317930919743324E-4	0.0	0.0	0.3839196022230693	0.0
2	6.317930919743324E-4	0.0	0.0	2.181370948890045	0.0
3	6.317930919743324E-4	0.0	0.0	2.9104601770284244	0.0
4	6.317930919743324E-4	0.0	0.0	3.9259622735285014	0.0
5	6.317930919743324E-4	0.0	0.0	6.861062381143925	0.0
6	6.317930919743324E-4	0.0	0.0	8.140653990089273	0.0
7	6.317930919743324E-4	0.0	0.0	9.595041687814186	0.0
8	8.423907892991098E-4	0.0	0.0	11.732397717963352	0.0
9	8.423907892991098E-4	0.0	0.0	12.456853796760587	0.0
10	8.423907892991098E-4	0.0	0.0	14.777851018976959	0.0
11	8.423907892991098E-4	0.0	0.0	16.9387939912265	0.0
12	8.423907892991098E-4	0.0	0.0	19.469335922281026	0.0
13	8.423907892991098E-4	0.0	0.0	20.19147542640769	0.0
14	8.423907892991098E-4	0.0	0.0	20.479573076347982	0.0
15	8.423907892991098E-4	0.0	0.0	21.087989823919266	0.0
16	8.423907892991098E-4	0.0	0.0	22.212160332238927	0.0
17	8.423907892991098E-4	0.0	0.0	23.49996525137994	0.0
18	8.423907892991098E-4	0.0	0.0	25.024903177708655	0.0
19	8.423907892991098E-4	0.0	0.0	25.931526264691822	0.0
20	8.423907892991098E-4	0.0	0.0	26.729480939855403	0.0
21	8.423907892991098E-4	0.0	0.0	27.727082232082875	0.0
22	8.423907892991098E-4	0.0	0.0	28.738793570031106	0.0
23	8.423907892991098E-4	0.0	0.0	29.727128563576287	0.0
24	8.423907892991098E-4	0.0	0.0	30.484016687761535	0.0
25	8.423907892991098E-4	0.0	0.0	31.17098637643496	0.0
26	8.423907892991098E-4	0.0	0.0	31.783404480255413	0.0
27	8.423907892991098E-4	0.0	0.0	32.410143227493954	0.0
28	8.423907892991098E-4	0.0	0.0	33.04004094019236	0.0
29	8.423907892991098E-4	0.0	0.0	33.681732123940954	0.0
30	8.423907892991098E-4	0.0	0.0	34.42619498398405	0.0
31	8.423907892991098E-4	0.0	0.0	35.05082775424933	0.0
32	0.0010529884866238872	0.0	0.0	35.6234428932754	0.0
33	0.0010529884866238872	0.0	0.0	36.19184607835498	0.0
34	0.0010529884866238872	0.0	0.0	36.740874275280675	0.0
35	0.0010529884866238872	0.0	0.0	37.38677741297577	0.0
36	0.0010529884866238872	0.0	0.0	37.99666834442832	0.0
37	0.0010529884866238872	0.0	0.0	38.55812180549618	0.0
38	0.0010529884866238872	0.0	0.0	39.13515949616607	0.0
39	0.0010529884866238872	0.0	0.0	39.74905178386779	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	30	2.1628002E-6	45.000004	10
ACACGCG	35	1.209919E-7	45.000004	36
TCTAGCG	30	2.1628002E-6	45.000004	1
CGTTAGT	30	2.1628002E-6	45.000004	12
TATTACG	35	1.209919E-7	45.000004	1
GCGGCTA	30	2.1628002E-6	45.000004	24
CGATACG	30	2.1628002E-6	45.000004	10
TAATACG	25	3.8873353E-5	45.0	4
GTCGATG	20	7.0291007E-4	45.0	1
GATCGTT	20	7.0291007E-4	45.0	10
GAATCGG	25	3.8873353E-5	45.0	2
TTCGTAA	20	7.0291007E-4	45.0	13
CGCGACT	25	3.8873353E-5	45.0	27
ACGGGCC	45	3.8380676E-10	45.0	5
ACTAGCG	25	3.8873353E-5	45.0	1
GTCAACG	25	3.8873353E-5	45.0	1
GTCCGGC	20	7.0291007E-4	45.0	44
CATACGA	40	6.8012014E-9	45.0	18
ACATCGC	20	7.0291007E-4	45.0	15
CCAGTCG	25	3.8873353E-5	45.0	28
>>END_MODULE